Sarcophagidae and Calliphoridae are families of Diptera that cover, respectively, about 3,100 and 1,000 species described worldwide. The species of these families have medical and veterinary importance, due to their role as vectors of pathogenic agents. The Diptera fauna is little known in many Brazilian ecosystems, including some phytophysiognomies of the Atlantic Forest. In the present study, two expeditions were carried out, one in the rainy season and the other in the dry season, using four traps baited with fish and exposed for 48 hours in the field in the Parque Natural Municipal Atalaia in the municipality of Macaé. The collected material was sorted and assembled at Instituto de Biodiversidade e Sustentabilidade - NUPEM/UFRJ and transported to Laboratório de Biodiversidade e Sistemática de Diptera - Museu Nacional, UFRJ (MNRJ), for later identification. A total number of 712 specimens of Calliphoridae and 27 of Sarcophagidae were collected. The sampling effort for Sarcophagidae collection was average, since the species accumulation curve continues to rise, unlike the Calliphoridae curve, which remains stable. The study included species considered asynanthropic, as Hemilucilia semidiaphana (Rondani, 1850) (Calliphoridae), but also invasive species, like those of the genus Chrysomya Robineau-Desvoidy, 1830 (Calliphoridae). Therefore, the present study contributed to expand knowledge about both families in the Atlantic Forest, in Rio de Janeiro, and emphasized the importance of continuing studies in the region, as many species are asynanthropic, while others are invasive, which can result in the inhibition of the native ones.