To characterize the 22:6 n-3 (docosahexaenoic acid, DHA) levels of the oriental bonito Sarda orientalis, a coastal migratory tuna species, total lipids (TL) of white muscle, dark muscle, liver, pyloric cecum, gonad, and other viscera were separated into lipid classes, the constituents of TL were quantified, and the fatty acid composition of TL, triacylglycerols (TAG), phosphatidylethanolamine, and phosphatidylcholine was analyzed. The crude lipid contents of muscle and other organs were 0.8–3.2% and 2.3–9.1%, respectively. DHA was found in TL of various organs at levels ranging from 19.2% to 27.6% in muscle and 16.3% to 28.5% in other organs. The levels of DHA in muscle TAG (8.2–16.0%) were lower than or comparable to those in visceral TAG (6.9–24.0%). These findings did not coincide with those observed in active-migratory tuna species, which accumulate DHA in their muscle TAG during migration. These findings suggest that the DHA distribution of S. orientalis is different from that observed in active-migratory tuna species, and that the differences may be due to migration type of the fish.