β‐caryophyllene is a dietary cannabinoid and it is a major plant volatile found in large amounts in the essential oils of many different spice and food plants, such as oregano (Origanum vulgare L.), cinnamon (Cinna‐momum spp.) and black pepper (Piper nigrum L.). The analgesic activity of β‐caryophyllene‐complexed in β‐cyclodextrin (CD‐CAR) was assessed in an animal model of fibromyagia (chronic non‐inflammatory muscle pain). Male Swiss mice were pretreated with CD‐CAR (10 or 20 mg/kg, p.o.) or vehicle (neutral saline). They were screened for mechanical hyperalgesia of the paw and muscle (von Frey and tweezer), motor coordination (Rota Rod) and muscle strength (Grip Strength Meter) beginning 60 min after treatment and continuing through 15 daysCD‐CAR, all doses, induced a significant reduction (p<0.05, p<0.01 or p<0.001) of mechanical hyperalgesia of the paw nd muscle in the chronic non‐inflammatory muscle pain model that lasted for 15 days. CD‐CAR produced no significant change in motor coordination or muscle strength. Therefore, we show that CD‐CAR reduces pain behaviors in an animal model for fibromyagia.Financial Support: CNPq/Brazil, FAPITEC/Brazil and FINEP/Brazil