Muscle Disease In Lambs Research Articles

Determination of local expressions of IGF-1, LC3B and NF-kB in white muscle disease in lambs by immunohistochemical method

White muscle disease (WMD) is also known as Stiff Lamb Disease or Nutritional Muscular Dystrophy. Selenium and/or Vitamin E deficiency constitutes the etiology of the disease. This study aimed to immunohistochemically evaluate local protein expressions of Nuclear factor kappa B (NF-kB), Insulin-like growth factor-1 (IGF-1) and Microtubule-related protein 1A/1B-light chain 3 beta (LC3B) in WMD. The material of the study consisted of 15 WMD, and 6 healthy lamb heart samples. The heart tissues of the autopsied lambs were subjected to routine tissue processing and paraffin blocks were obtained. Then, it was stained with Hematoxylin-Eosin and immunohistochemical methods. Control group lambs had normal macroscopic appearance. Macroscopically, hyaline degeneration and zenker’s necrosis, calcification areas were observed in WMD tissues. Microscopically, degenerative and necrotic muscle fibers, calcification areas, fibrosis, mononuclear cell infiltrates and macrophage infiltrates were detected in WMD heart tissues. Immunohistochemically, significant increases were detected in IGF-1 (p

Pharmacological therapy of white muscle disease in lambs with selenium preparations in a comparative aspect

In the course of the experiment on the comparative assessment of selenium-containing preparations selephlan and plexomin Se-2000 in the treatment of white muscle disease in young sheep, it was determined that selephlan has a more pronounced effect on the clinical and physiological state and intestinal biocenosis of lambs, their morphological and biochemical blood parameters, as well as the level of antioxidant protection of the body of animals. Under the influence of the preparation, the erythro- and hematopoiesis is activated in lambs, as well as the normalization of biochemical blood homeostasis. The level of enzymatic activity (AST, ALT, ALP, LDH) significantly decreases in 1.36 (p≤0.05); 1.71 (p≤0.001); 1.21 (p≤0.05) and 1.3 (p≤0.01) times, while the concentration of carotene increases in 3.1 times, vitamin E increases in 1.73 times and vitamin A increases by 26.8%. Selephlan helps to reduce the concentration of DC by 21.8%, KD – by 28.7%, MDA – in 1.61 (p <0.05) times.

Expression of ADAMTS-7 in myocardial dystrophy associated with white muscle disease in lambs.

The aim of the present study was to investigate the role of ADAMTS-7 gene in the pathogenesis of myocardial dystrophy associated with white muscle disease (WMD) in lambs. A total of 217 cardiac tissue samples from lambs with WMD were used in the study. Histopathological sections of the samples were stained with hematoxylin-eosin (HE) and examined using Western-blot, real-time PCR (RT-PCR) and immunohistochemistry for ADAMTS-7 gene expression, and the findings were statistically evaluated. Histopathological examinations revealed fibrosis associated with hyalinization, necrosis and granular calcifications in cardiomyocytes. Western blot and RT-PCR showed a statistically significant upregulation of ADAMTS-7 (p<0.05) (p<0.05). Immunohistochemical analyses showed that immunopositive cell numbers significantly high for ADAMTS-7 (p<0.05). The study has revealed that ADAMTS-7 gene is significantly expressed in myocardial dystrophy associated with WMD in addition to its role in the pathogenesis of this disease.

Effect of selenium on the reproductive performance of ewes and white muscle disease in lambs

A study using 60 Awassi ewes fed either a control (unsupplemented) diet or one containing selenium-enriched yeast (Selplex™, Alltech Incorporated, USA) at a dose rate of 0.2 mg/kg diet was conducted to investigate the effects on white muscle disease occurrence in progeny. The effects of organic selenium on oestrus, fertility, lamb birth weights, weaning weights and rates of white muscle disease were recorded. There was no effect of organic selenium supplementation on pregnancy rates as recorded by ultrasound at 40 and 80 days after mating (P>0.05). Likewise, there were no significant differences in lambing, single or twinning rates between the control ewes and those receiving organic selenium. Birth and weaning weights were not significantly different in either single or twin lambs from either control or Se-supplemented ewes. However, the Se-supplemented ewes gave birth to lambs which had significantly less incidence of white muscle disease (37.5% versus 3.3%; P

Omental fat tissue influence on bone healing: Radiological and histomorphometric study

Reid found selenium (Se) deficient soils were a common factor in two populations with the highest incidence of SIDS world-wide (Indian population of King County Washington State, USA and the population of Canterbury, New Zeland) (1).Reid compared a map of the selenium responsive livestock areas in New Zealand with areas of low soil iodine (I). She found that the 1989 report of areas of the highest SIDS incidence coincided with Se responsive livestock areas combined with low soil iodine (2–4).Foster found the 1983–1984 incidence of SIDS in USA has the strongest positive correlation with the incidence of goitre in World War I troops (1916). Sodium and strontium were positively related (5).Emery found a hypernatraemic SIDS victim in a very cold environment (6). Robertson and Parker associated increased sodium (Na) (used in Scunthorpe, England, as a water softener) with increased incidence of SIDS (7).Godwin's study of White Muscle Disease in lambs describes Se protection (8). Skeletal muscle mitochondria from dystrophic animals showed lowered respiratory rates with palmitoyl-dl-carnitine and acetyl-dl-carnitine as substrate. Dystrophic organelles failed to respond to ADP.Rognun found elevated hypoxanthine and an enhanced immune response in most SIDS victims (9).A syndrome associated with potassium deficiency has been described as a killer of healthy, young Asian men, most often during sleep (19).This paper describes the interactions of sodium (Na), potassium (K), selenium (Se), and iodine (I) to some factors affecting the utilization of oxygen and the production of energy.

SELENIUM, TRANSAMINASE AND WHITE MUSCLE DISEASE IN LAMBS

Journal Article Selenium, Transaminase and White Muscle Disease in Lambs Get access Nutrition Reviews, Volume 19, Issue 6, June 1961, Pages 169–170, https://doi.org/10.1111/j.1753-4887.1961.tb01952.x Published: 01 June 1961

The Importance of Serum Creatine Kinase(CK),Aspartate Aminotransferase(AST) and Lactate Dehydrogenase(LDH) Activities in the Diagnosis and Prognosis of Subclinic and Clinic White Muscle Disease in Lambs

In this study ; the importance of serum CK, AST and LDH activities in the diagnosis and prognosis of subclinic and clinic White Muscle Disease in lambs was searched in consideration of the effect of vitamin E+selenium application on these enzyme values. Akkaraman 26 lambs (8 healthy, 8 subclinically and 10 clinically affected with White Muscle Disease) were used in investigation. Serum CK, AST and LDH activities were measured according to kinetic procedure with commercial test kits. Serum CK, AST and LDH activities were significantly higher in lambs with subclinical and clinical White Muscle Disease compared to healthy lambs (p

Sudden infant death syndrome (SIDS): Oxygen utilization and energy production

Reid found selenium (Se) deficient soils were a common factor in two populations with the highest incidence of SIDS world-wide (Indian population of King County Washington State, USA and the population of Canterbury, New Zeland) (1). Reid compared a map of the selenium responsive livestock areas in New Zealand with areas of low soil iodine (I). She found that the 1989 report of areas of the highest SIDS incidence coincided with Se responsive livestock areas combined with low soil iodine (2–4). Foster found the 1983–1984 incidence of SIDS in USA has the strongest positive correlation with the incidence of goitre in World War I troops (1916). Sodium and strontium were positively related (5). Emery found a hypernatraemic SIDS victim in a very cold environment (6). Robertson and Parker associated increased sodium (Na) (used in Scunthorpe, England, as a water softener) with increased incidence of SIDS (7). Godwin's study of White Muscle Disease in lambs describes Se protection (8). Skeletal muscle mitochondria from dystrophic animals showed lowered respiratory rates with palmitoyl-dl-carnitine and acetyl-dl-carnitine as substrate. Dystrophic organelles failed to respond to ADP. Rognun found elevated hypoxanthine and an enhanced immune response in most SIDS victims (9). A syndrome associated with potassium deficiency has been described as a killer of healthy, young Asian men, most often during sleep (19). This paper describes the interactions of sodium (Na), potassium (K), selenium (Se), and iodine (I) to some factors affecting the utilization of oxygen and the production of energy.

子羊の白筋症に関する臨床病理学的観察

子羊の白筋症に関する臨床病理学的観察

Selenium in soils, pastures and animal tissues in relation to the growth of young sheep on a marginally selenium-deficient area

Abstract Extract Selenium-responsive diseases of farm animals in New Zealand have been reviewed by Andrews et al. (1968). In sheep these may be manifested as infertility in ewes, as “congenital” or “delayed” white muscle disease in lambs, or as unthriftiness, a condition usually more apparent in young animals. As evidenced by weight responses of lambs to dosing with selenium salts, selenium-responsive unthriftiness has occurred widely in New Zealand (Robertson and During, 1961). Most investigations into selenium-responsive conditions, e.g., Cousins and Cairney, 1961; Hartley, 1967; A. B. Grant, pers. comm.) have related mainly to areas that could be described as severely selenium-deficient.

Some biochemical features of white muscle disease in lambs, and the influence of selenium.

Typical white muscle disease has been induced in lambs that were normal at weaning but thereafter fed natural dystrophogenic fodder for 2-4 months. Protection from the disease was given by selenium when it was administered regularly as an oral drench, or incorporated into an intraruminal heavy pellet placed in the rumen at weaning.

Selenium binding proteins in tissues of normal and selenium responsive myopathic lambs

Normal and selenium-responsive myopathic (white muscle disease) lambs were injected with selenium-75 either as sodium selenite or seleno-methionine. Selected tissues were removed at autopsy 16 hr after injection. The soluble fraction from homogenates of these tissues was subjected to gel chromatography and the effluent monitored for radioactivity and protein content. The elution curves were similar for both sodium selenite and selenomethionine injections. Selenium was bound to two different molecular weight proteins in the kidney and three different molecular weight proteins in the liver, pancreas, and plasma. Four different molecular weight selenium-binding proteins were found in heart and semitendinosus muscle of 2-week-old normal lambs, but only three were found in these tissues of myopathic lambs. This fourth protein, which was absent in myopathic lambs, corresponded to a molecular weight of approximately 10,000. However, by six weeks of age rudimentary amounts of this protein were present in the tissues of the myopathic lamb. This protein may be implicated in the prevention of white muscle disease in lambs.

Influence of Sulfur on Incidence of White Muscle Disease in Lambs ,

Two trials were conducted with sheep to investigate the effect of sulfur on the incidence of white muscle disease (WMD). Neither methionine nor sulfate increased the incidence of WMD in either trial, but sulfate significantly increased the number of lambs with degenerative lesions of the heart. There was a fivefold increase of plasma glutamic-oxaloacetic transaminase (GOT), lactic dehydrogenase (LDH) and malic dehydrogenase (MDH) of the lambs at 2 weeks of age with the nonsupplemented ewes as compared with those from ewes receiving selenium. The GOT, LDH and MDH levels of the lambs from ewes fed sulfate or methionine were twofold greater than comparable values of the control lot. At 6 weeks of age, however, the levels of these enzymes of the sulfate and methionine lots had approached the levels of the nonsupplemented group. White muscle disease had no effect on blood glucose, lactate or choleserol. Based on the enzyme levels, the sulfur sources appeared to delay the onset of WMD. A significant “carryover” of selenium was noted.

Selenium and vit. E as related to growth and white muscle disease in lambs.

Summary1) Selenium, given pre-natally per os to ewes or post-natally by injection to lambs, prevented WMD and resulted in increased growth of lambs as compared to untreated controls. 2) Growth response to selenium fed pre-natally was greater than that to selenium injected post-natally. 3) Massive doses of Vit. E at birth protected lambs from WMD, but did not result in improved growth. 4) Evidence suggests an essential role for selenium in supporting growth of lambs.

Further observations on white muscle disease in lambs

Abstract Extract This paper describes further observations on the incidence of, and factors connected with, white muscle disease in lambs. It supplements previous papers by Hartley (1953) Hartley, W. J. 1953. N.Z. vet. J., 1: 137–137. [Taylor & Francis Online] , [Google Scholar] and Hartley and Dodd (1957) Hartley, W. J. and Dodd, D. C. 1957. N.Z. vet. J., 5: 61–61. [Taylor & Francis Online] , [Google Scholar].

Selenium and animal health

Abstract Extract The value of α-tocopherol in the prevention, control, and cure of white muscle disease in lambs has been investigated in New Zealand with inconelusive results (Hartley, pers comm.).