Objective: To investigate the effect of different arterial occlusion pressure and intermittent time on KAATSU-loaded deep-squat exercise on the characteristics of thigh muscle activation, find out the suitable relative value of personal blood pressure limit causing the maximum activation, and to provide some theoretical basis and practical reference for athletes to carry on the KAATSU Training scientifically. Methods: Ten elite male players were recruited, four kinds of external pressure condition with 0% arterial occlusion pressure (AOP), 40%AOP, 50%AOP, 60%AOP, respectively, were performed with 30%1RM(One repetition maximum)intensity of intermittent and continuous KAASTU-loaded deep-squat exercise. Wave plus wireless surface electromyography instrument was used to collect the surface EMG signals of the lower thigh muscle group. Root mean square (RMS) calculation was used to estimate muscle activity level. The normalized RMS values of the anterior thigh group and posterior thigh group were calculated by RMS of MVC, two-factor ANOVA analysis of variance were used to explore the effect of the activation of various muscle on external express and intermittent mode, to analyze the discrepancy during different external pressure. Results: ①A two-factor analysis of variance showed that different %AOP had significant effect on the normalized values of RMS of muscle measured by KAATSU deep-squat exercise(P<0.05), but intermittent mode had no significant effect on the standard value of RMS of muscle(P>0.05). The interaction of external pressure and intermittent mode on muscle RMS was not significant(P>0.05); ②The normalized RMS values of Rectus Femoris, Vastus Medialis, Vastus Lateralis, Biceps Femoris, Semitendinosus were significantly increased (P<0.05)in 50% AOP pressure condition during intermittent and continuous exercise; ③ The normalized RMS values of RF and VL were significantly increased(P<0.05)in 50% AOP pressure condition during intermittent and continuous exercise. The %MVC values of Vastus Medialis and Semitendinosus were significantly increased(P<0.05)in 60% AOP pressure condition during intermittent exercise. Conclusion: This study further verified that 50% AOP pressure can significantly improve the optimal activation quadriceps and posterior thigh groups and produce the best training effect for the high level handball players. It can not only promote the coordinated development of the agonist and antagonist, but also avoid overloading and prevent hamstring injury. At the same time, the intermittent mode is recommended to adopt the decompression intermittent mode.
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