Indian Muntjac Research Articles

Predicting the Impact of Climate Change on the Selection of Reintroduction Sites for the South China Tiger (Panthera tigris amoyensis) in China.

The South China tiger (Panthera tigris amoyensis) is a tiger subspecies unique to China and one of the top ten endangered species in the world. It used to play an important role in the overall function of the ecosystem. This study rationally screened out key prey species of the South China tiger-the Chinese serow, Chinese goral, tufted deer, water deer, Chinese muntjac, red muntjac, sambar deer, and wild boar. Candidate sites for the rewilding and reintroduction of the South China tiger were derived by exploring changes in suitable habitats for the prey using the MaxEnt model. The results show that: (1) by 2070, except for the high-suitability areas of water deer and Chinese muntjac, the areas of suitable habitats for the other six prey species would all have decreased significantly; (2) the location of the high-suitability area of the South China tiger obtained by superimposing the suitable areas of the eight prey species would be almost stable in 2050 and 2070, but the habitat index of some high- and medium-suitability areas would decrease and turn into low-suitability areas; (3) the core candidate sites were 83,415 km2 in total, of which 25,630 km2 overlapped with existing protected areas, accounting for 30.7% of the core candidate sites, and the remaining 69.3% of the core candidate sites were mostly distributed around the protected areas; (4) the maximum core candidate site area was projected to be 10,000 km2 by 2070, which could support a small population of 23 male tigers and 66 female tigers to survive and reproduce in the wild. This study revealed the core candidate sites for the rewilding of South China tigers and estimated the number of tigers that could be reintroduced to these areas, providing a preliminary research basis for promoting the rewilding of South China tigers in China.

Landscape-level habitat connectivity of large mammals in Chitwan Annapurna Landscape, Nepal.

The populations of many species of large mammals occur in small isolated and fragmented habitat patches in the human-dominated landscape. Maintenance of habitat connectivity in the fragmented landscapes is important for maintaining a healthy population of large mammal. This study evaluated the landscape patches and their linkages on two carnivores (leopard and Himalayan black bear) and seven prey species (northern red muntjac, chital, sambar, wild pig, Himalayan goral, rhesus macaque, and langur) between Chitwan National Park (CNP) and Annapurna Conservation Area (ACA) by using the least-cost path (LCP) approach and the Linkage Mapper tool in ArcGIS. A total of 15 habitat patches (average area 26.67 ± 12.70 km2) were identified that had more than 50% of the total studied mammals. A weak relation among the habitat patches was found for chital and sambar (Cost-weighted distance [CWD]: Euclidean distance EucD >100), showed poor connectivity between the habitat patches, while ratio of CWD and EucD was low (i.e., low LCP) between majority of the patches for muntjac, wild pig and leopard hence had potential functional connectivity along the landscape. Similarly, low LCP between the habitat patches located in the mid-hills was observed for Himalayan goral and Himalayan black bear. Furthermore, the multi-species connectivity analysis identified the potential structural connectivity between the isolated populations and habitat patches. Therefore, these sites need to be considered connectivity hotspots and be prioritized for the conservation of large mammals in the landscape.

Fine-scale habitat suitability modelling of Northern red muntjac (Muntiacus vaginalis) in the Chitwan Annapurna Landscape, Nepal

Abstract Factors associated with the habitat suitability of northern red muntjac (Muntiacus vaginalis) especially outside protected areas in the human-dominated landscape are still lacking. Fine-scale environmental variables can influence the habitat suitability of the animals. This study aimed to explore the different eco-geographic fine-scale variables for the distribution of the northern red muntjac; and predict suitable habitats using the maximum entropy (Maxent) model in the Chitwan Annapurna landscape (CHAL). The presence points of the northern red muntjac (n = 265) were collected between 2018 to 2021 using 150 transects of various lengths in four blocks. Density-based occurrence points rarify and performance-based variable selection were applied to improve the output of the model. The model was evaluated based on the area under the curve (AUC) value of operator characteristic (ROC) and analyzed on the basis of the response curve, the relative importance of variables, Jackknife test and suitability map. Results indicated the model was statistically satisfactory (mean AUC > 0.75). The distance to the nearest cropland was the most contributed variable followed by Normalized Difference Built-up Index (NDBI), distance to developed/settlement area and distance to grassland that explained suitability of Northern red muntjac. The species distribution model predicted 6.52% highly suitable and 23.77% suitable area for northern red muntjac. Therefore, this area is important for the muntjac and provides a possible alternative habitat for other wild animals outside the protected areas. Our research suggests that human dominated landscape should be prioritized in management plans for the conservation of muntjac.

Exploring temporal activity of dholes, their prey, and competitors in East Java, Indonesia.

Dholes (Cuon alpinus) are endangered large carnivores found in scattered populations in Asia. One of the main threats to dholes is the decreasing prey availability throughout their distribution range. In the present study, we used camera trap data collected over 6 years to investigate the temporal activity patterns of dholes and their putative prey species in Baluran National Park in Java, Indonesia. We also explored the overlap in activity between dholes and the park's other remaining large carnivore the Javan leopard (Panthera pardus melas), as well as humans. Furthermore, we investigated potential differences in activity patterns between dholes in packs and dholes roaming in pairs or alone. We found a high temporal overlap between dholes and their wild ungulate prey species (ranging from Δ = 0.66-0.90), with the lowest overlap observed between dholes and bantengs (Bos javanicus) (Δ = 0.66), and the highest between dholes and muntjacs (Muntiacus muntjak) (Δ = 0.90). A very low overlap was found between dholes and domestic cattle (Bos indicus) (Δ = 0.27) whereas a moderately high overlap was found between dholes and leopards (Δ = 0.70) and dholes and humans (Δ = 0.62). We found a significant difference in activity patterns between dholes in packs and dholes roaming alone or in pairs (Δ = 0.78, p = .01). Single/pairs of dholes were more active both during the day and at night, whereas packs were predominantly active around sunrise and sunset. The high overlap with humans potentially has a negative effect on dhole activity, particularly for dispersing individuals, and the low overlap with domestic species questions the extent to which dholes are considered to predate on them.

Using feces to indicate plastic pollution in terrestrial vertebrate species in western Thailand.

Plastic pollution is a widespread and growing concern due to its transformation into microplastics (MPs), which can harm organisms and ecosystems. This study, aimed to identify plastic pollution in the feces of terrestrial vertebrates using convenience sampling both inside and outside protected areas in Western Thailand. We hypothesized that MPs are likely to be detectable in the feces of all vertebrate species, primarily in the form of small black fragments. We predicted varying quantities of MPs in the feces of the same species across different protected areas. Furthermore, we expected that factors indicating human presence, landscape characteristics, scat weight, and the MP abundance in water, soils, and sediments would influence the presence of plastics in feces. Among 12 terrestrial species studied, potential MPs were found in 41.11% of 90 samples, totaling 83 pieces across eight species including the Asian elephant (Elephas maximus), Eld's deer (Rucervus eldii), Dhole (Cuon alpinus), Gaur (Bos gaurus), Sambar deer (Rusa unicolor), Wild boar (Sus scrofa), Northern red muntjac (Muntiacus vaginalis), and Butterfly lizard (Leiolepis belliana). Specifically, 3.61% of all potential MPs (three pieces) were macroplastics, and the remaining 96.39% were considered potential MPs with the abundance of 0.92 ± 1.89 items.scat-1 or 8.69 ± 32.56 items.100 g-1 dw. There was an association between the numbers of feces with and without potential plastics and species (χ2 = 20.88, p = 0.012). Most potential plastics were fibers (95.18%), predominantly black (56.63%) or blue (26.51%), with 74.70% smaller than two millimeters. Although there were no significant associations between species and plastic morphologies, colors, and sizes, the abundance classified by these characteristics varied significantly. FTIR identified 52.38% as natural fibers, 38.10% as synthetic fibers (rayon, polyurethane (PUR), polyethylene terephthalate (PET), polypropylene (PP), and PUR blended with cotton), and 9.52% as fragments of PET and Polyvinyl Chloride (PVC). Human-related factors were linked to the occurrence of potential plastics found in the feces of land-dwelling wildlife. This study enhances the understanding of plastic pollution in tropical protected areas, revealing the widespread of MPs even in small numbers from the areas distant from human settlements. Monitoring plastics in feces offers a non-invasive method for assessing plastic pollution in threatened species, as it allows for easy collection and taxonomic identification without harming live animals. However, stringent measures to assure the quality are necessitated to prevent exogenous MP contamination. These findings underscore the importance of raising awareness about plastic pollution in terrestrial ecosystems, especially regarding plastic products from clothing and plastic materials used in agriculture and irrigation systems.

Range-wide camera traps reveal potential prey species for Javan leopards

Prey depletion poses a threat to large carnivores worldwide. The Javan leopard (Panthera pardus melas), the last remaining apex predator on the Indonesian island of Java, is facing numerous threats from human activities including poaching, habitat loss and prey depletion. Despite the fact that the Javan leopard is an adaptable and opportunistic predator capable of surviving in various environments, our understanding of its prey and predator-prey interactions is still limited, which is crucial for their conservation. Using camera trap data collected from four national parks (i.e., Ujung Kulon, Gunung Gede Pangrango, Meru Betiri, Alas Purwo) that represent four distinct terrestrial ecoregions across the island of Java, we investigated the species richness, relative abundance, and spatial-temporal overlap in activity patterns between the Javan leopard and other animals that coexist in the same location. Our analysis of 7461 independent photos, covering 12,983 camera trap days, revealed that areas with the presence of Javan leopards exhibited higher species richness and abundance compared to those without the presence of Javan leopards. In addition, we found that the activities of banteng (Bos javanicus), barking deer (Muntiacus muntjak), Javan deer (Rusa timorensis), Javan mousedeer (Tragulus javanicus), wild boar (Sus scrofa), junglefowl (Gallus spp.), spangled ebony langur (Trachypithecus auratus), Javan rhino (Rhinoceros sondaicus), long-tailed macaque (Macaca fascicularis) and dhole (Cuon alpinus) were highly overlapping with those of Javan leopards in space and time, suggesting that these species are potential candidate prey for Javan leopards. To our knowledge, this is the first comprehensive investigation of potential prey for the Javan leopard that utilizes camera traps from all four types of terrestrial ecoregions on Java Island. The findings of this study may serve as essential information for the conservation of this critically endangered species.

First record of mutual allogrooming in wild northern red muntjac Muntiacus Vaginalis

First record of mutual allogrooming in wild northern red muntjac Muntiacus Vaginalis

A tapestry of habitats: exploring abundance and habitat preferences of the Northern Red Muntjac (Muntiacus vaginalis) across the Central Himalayan landscape

Abstract Understanding the occupancy and density estimate of Northern Red Muntjac (Muntiacus vaginalis) at a landscape scale is crucial due to their significant ecological impact on ecosystems, particularly as prey for large predators. This study employed a single-season site occupancy model to assess the occupancy and density of Northern Red Muntjac using camera traps in the east Sikkim and Darjeeling hills Central Himalayan landscape from 2018 to 2020. A total of 513 photo captures of Northern Red Muntjac were obtained from 3150 trap nights with a naive occupancy estimate of 0.64 for the Central Himalayan landscape. The top model revealed positive influences of broadleaved forest (β = 0.93 ± 0.26), mixed forest (β = 0.6 ± 0.47), and slope (β = 0.2 ± 0.24) on the occupancy probability, while elevation (β = −0.08 ± 0.24) and ruggedness (β = −0.53 ± 0.23) had negative influences. Similarly, broadleaved forest (β = 1.3 ± 0.24), mixed forest (β = 0.74 ± 0.39), and ruggedness (β = 0.62 ± 0.25) positively influenced the detection probability, whereas elevation (β = −0.3 ± 0.22) and slope (β = −1.13 ± 1.21) had negative effects. The study estimated a total abundance of 169.23 ± 20.77 individuals with a density of 0.33 per square kilometre in the landscape. This study is a pioneering effort and provides a foundation for future investigations into the occupancy and density estimate of Northern Red Muntjac in the Central Himalayas.

Variation in water utilization by mammal diversity in Khao Phaeng Ma Non-hunting area, Thailand

Access to suitable water sources is important for mammals. This study aimed to compared mammal diversity and water use among water springs, standard artificial ponds, and water pans within the Khao Phaeng Ma Non-Hunting Area in 2020 and 2021. Two camera traps were installed at each water sources for 749 nights with a total of 12 camera traps of 6 water sources. A total of 19,467 photographs were recorded comprising 13,777 photographs of gaur (Bos gaurus, vulnerable and the most important species in the area), and 5690 photographs of other mammals. In the wet season, relative use was highest at standard artificial pond number 2, which is established in the forest plantation area (4 × 4 m spacing, 12–20 m height, and 60%–80 % crown cover) and has a high volume of water, and at water pan number 1, which mimics a natural water spring in the man-made grassland and can supply water to mammals throughout the year. In the dry seasons, relative use was highest at water pan numbers 1 and 2; at the same time, other water sources dried up. During the study period, the number of mammal species was highest at water pan number 1 (10 species, diversity index [H′] = 1.38), and water pan number 2 (11 species, H′ = 1.75). Grazers and browsers, including gaur, sambar deer (Rusa unicolor), northern red muntjac (Muntiacus vaginalis), omnivores (e.g. wild boar, Sus scrofa), and Asian black bear (Ursus thibetanus), used the water pan in the artificial grassland and standard artificial pond in the forest plantation rather than the water spring in the dry evergreen forest. Beside forest types, the use of water springs was associated with water period (months), while the use of standard artificial pond and water pans was associated with water surface area, water depth, altitude, species diversity, and species richness, and number of mammals photographed. The results show that water pans were more suitable for utilization by mammals than are other water sources.

Site occupancy of select mammals in the tropical forest of Eastern Himalaya

IntroductionTerrestrial mammals play a pivotal role in tropical forest ecosystems, representing a rich and functionally diverse component of these biomes. However, they confront formidable threats globally, such as hunting, habitat loss, and fragmentation, amidst the expansion of human-altered landscapes in tropical regions. Understanding the impacts of these changes on mammal communities is crucial for designing effective conservation interventions. Northeast India's tropical forests, a biodiversity hotspot, host a diverse array of mammalian fauna. The primary challenge faced by these species involves anthropogenic activities leading to the shrinkage and fragmentation of forested habitats.MethodologyIn this study, we conducted systematic camera trapping in Mehao Wildlife Sanctuary (WLS) to assess mammal status and distribution. Employing single-season occupancy models, we evaluated factors influencing mammalian species' detection probability and spatial utilization within the landscape. ResultsThe study documented 27 mammalian species, with 12 identified as "threatened" on the IUCN Red List. Species like clouded leopard, marbled cat, Gongshan muntjac, red goral, Mishmi takin, and sambar were recorded but observed infrequently. Notably, we reported the Asiatic brush-tailed porcupine's first sighting in Mehao. Our findings revealed that undisturbed forest habitats were preferred by all species. Mainland serow dominated most of the sanctuary's area, followed by northern red muntjac, yellow-throated marten, and masked palm civet.DiscussionThe response of mammalian species to ecological and anthropogenic covariates varied significantly, underscoring the necessity for species-specific management strategies alongside a landscape-scale conservation approach. These outcomes emphasize the urgency of managing and safeguarding rare and elusive mammalian species within and around Mehao WLS.

Correlates and impacts of human-mammal conflict in the central part of Chitwan Annapurna Landscape, Nepal

Crop damage, predation on domestic animals and human attacks are often associated with human-wildlife conflict. The abundance and encounter rate of wild mammals are often associated with human wildlife conflict. Crop damage, livestock depredation and human injury and their relation with environmental factors and encounter rate of the mammals was evaluated in the central region of Chitwan Annapurna Landscape. The abundance and the encounter rate of large mammals were determined by dividing the study area into four different study blocks (A, B, C and D) based on river course and topography. A total of 150 transects (average length = 3.18 ± 0.11 km), were administrated for data collection. Similarly, information on human-wildlife conflict was collected using 600 semi-structured questionnaires (150 from each block). The chital was the most abundant mammal (encounter rate (ER) = 1.49 and relative abundance (RA) = 55.45%) in block A whereas muntjac had the highest encounter rate in blocks B, C and D (ER = 0.34, 0.31, 0.79 respectively) but the relative abundance of rhesus was comparatively higher in blocks B, C and D. The signs of tiger were reported from block A only whereas signs of leopard were reported from all blocks. But signs of Himalayan black bear were reported from B, C and D. The encounter rate of the mammals correlated with the monetary loss caused by them. Greater one-horned rhino, wild pig and chital in lowland and monkeys, northern red muntjac, and Himalayan black bear in mid-hills (blocks B, C and D) were the principal crop raiders with a total average annual loss of US$ 11.45 per household. Similarly, the total annual monetary loss by livestock depredation was US$ 76.60 per household. This study evaluated the encounter rate of mammals and their effects on the conflict with people. However, the negative effects arrived from human-wildlife conflicts always threaten large mammals. Hence, this study suggests future intensive studies related to mitigation and prevention methods to mitigate the issues of human-wildlife conflicts.

Prey selection by leopards (Panthera pardus fusca) in the mid-hill region of Nepal.

Information on prey selection and the diet of the leopard (Panthera pardus fusca) is essential for leopard conservation. We conducted an investigation into the prey species and the proportion of each species in the leopard's diet in a human-dominated mid-hill region of Nepal. The analysis of 96 leopard scats collected between August 2020 and March 2021 revealed that leopards consumed 15 prey species, including small- and medium-sized mammals and livestock. In addition to these prey species, we also found plastic materials, bird feathers, and some unidentified items in the leopard scats. Wild ungulates (such as barking deer, Muntiacus muntjak and wild boar, Sus scrofa) constituted only 10% of the biomass in the scats, while livestock contributed 27%, and other wild prey contributed 50%. Among all species, domestic goats had the highest relative biomass in the scats, followed by the jungle cat (Felis chaus), domestic dog (Canis familiaris), and large Indian civet (Viverra zibetha). Similarly, the Indian hare (Lepus nigricollis) had the highest proportion of relative individuals present in the scat samples, followed by the jungle cat and the large Indian civet. A lower proportion of biomass from wild ungulates in the leopard's diet and a higher dependency of the leopard on domestic prey and other wild prey indicate a shortage of medium-sized wild prey, such as barking deer and wild boar, in leopard habitats. Therefore, the conservation of wild prey species, especially medium-sized prey, is crucial for reducing the leopard's dependence on livestock and mitigating human-leopard conflicts in the future.

Animal Density Estimation for Large Unmarked Populations Using a Spatially Explicit Model

AbstractObtaining abundance and density estimates is a particularly important aspect within wildlife conservation and management. To monitor wildlife populations, the use of motion-sensor camera traps is becoming increasing popular due to its non-invasive nature. However, animal identification is not always feasible in practice due to poor quality images and/or individuals not having uniquely identifiable physical characteristics. Spatially explicit models for unmarked individuals permit the estimation of animal density when individuals cannot be uniquely identified. Due to the structure of these models, a Bayesian super-population (data augmentation) approach is often used to fit the models to data, which involves specifying some reasonably large upper limit for the population. However, this approach presents substantial computational challenges for larger populations, as demonstrated by the motivating dataset relating to barking deer (Muntiacus muntjak) collected in Ujung Kulon National Park, Indonesia (with a population size in the low thousands). We develop a new and computationally efficient Bayesian algorithm for fitting the models to data that does not require specifying an upper population limit a priori. We apply the new algorithm to the large barking deer dataset, where the standard super-population approach is computationally expensive, and demonstrate a substantial improvement in computational efficiency.Supplementary material to this paper is provided online.

Systematic camera trapping survey for terrestrial vertebrates in Xuan Lien Nature Reserve, Vietnam.

Xuan Lien Nature Reserve was established in 1999 to protect important habitats and wildlife in the northern part of the Annamites in Vietnam. While Xuan Lien is home to many threatened species, it has experienced a high level of human disturbance over the last decades. To document and provide baseline data on the status and distribution of the terrestrial vertebrate fauna community in the region, we conducted a systematic camera trapping survey in Xuan Lien Nature Reserve in 2023. The data collected during the survey will help design proper conservation measures to better conserve the remaining species. Our study investigates and updates the species richness of mid- to large-sized terrestrial vertebrates, thus providing essential information for developing conservation strategies in Xuan Lien Nature Reserve, Vietnam. As camera traps were set up in a grid-based design, our survey also generated the first-ever systematic data for terrestrial vertebrate fauna in the area. The study covers approximately 21,000 hectares (about 77% of the area), using 35 camera trap stations; each station consists of two cameras. In total, the final dataset consists of 6,276 trap nights, recording at least 46 species in 39 genera. The results suggest that Xuan Lien is a key stronghold for small carnivores, based on the diversity of such species groups in the Reserve. We failed to detect the previously documented Roosevelt's muntjac (Muntiacusrooseveltorum Osgood, 1932) and we only documented a single record of the northern red muntjac (Muntiacusvaginalis Boddaert, 1785). Our survey confirms the severity of overhunting and other anthropogenic threats to the mammal fauna, especially ungulates, in the Reserve.

Hi-C sequencing unravels dynamic three-dimensional chromatin interactions in muntjac lineage: insights from chromosome fusions in Fea's muntjac genome.

Eukaryotes have varying numbers and structures of characteristic chromosomes across lineages or species. The evolutionary trajectory of species may have been affected by spontaneous genome rearrangements. Chromosome fusion drastically alters karyotypes. However, the mechanisms and consequences of chromosome fusions, particularly in muntjac species, are poorly understood. Recent research-based advancements in three-dimensional (3D) genomics, particularly high-throughput chromatin conformation capture (Hi-C) sequencing, have allowed for the identification of chromosome fusions and provided mechanistic insights into three muntjac species: Muntiacus muntjak, M. reevesi, and M. crinifrons. This study aimed to uncover potential genome rearrangement patterns in the threatened species Fea's muntjac (Muntiacus feae), which have not been previously examined for such characteristics. Deep Hi-C sequencing (31.42 × coverage) was performed to reveal the 3D chromatin architecture of the Fea's muntjac genome. Patterns of repeated chromosome fusions that were potentially mediated by high-abundance transposable elements were identified. Comparative Hi-C maps demonstrated linkage homology between the sex chromosomes in Fea's muntjac and autosomes in M. reevesi, indicating that fusions may have played a crucial role in the evolution of the sex chromosomes of the lineage. The species-level dynamics of topologically associated domains (TADs) suggest that TAD organization could be altered by differential chromosome interactions owing to repeated chromosome fusions. However, research on the effect of TADs on muntjac genome evolution is insufficient. This study generated Hi-C data for the Fea's muntjac, providing a genomic resource for future investigations of the evolutionary patterns of chromatin conformation at the chromosomal level.

Inventory of Large Mammals in Ujung Kulon National Park, Banten Province

The National Park has a diverse ecosystem for the preservation of flora and fauna, making Ujung Kulon National Park a habitat for endemic and protected fauna. Large mammals are one of the many animals found in TNUK, especially those with protected status. This study aims to determine the diversity of large mammal species in Ujung Kulon National Park. The research was conducted on September 27–29, 2022, in Ujung Kulon National Park, which is located at the western tip of Java Island, precisely in Sumur and Cimanggu Districts, Pandeglang Regency, Banten Province. The method used was the cruising method using exploratory descriptive data analysis. The results showed that there were two types of large mammals, namely ungulate mammals and primate mammals. The number obtained was 10 types of mammal species belonging to 7 different families. Large mammals found include Rhinoceros sondaicus, Muntiacus muntjak, Cervus timorensis, Hyolobates moloch, Presbytis comata, Trachypitecus auratus, Macaca fascicularis, Sus scrofa, Tragulus javanicus, and Bubalus bubalis. Most of the large mammals found are endemic mammals with protected status. Of the large mammals found, the most common were hoofed mammals. With this study, the public has an awareness of the importance of maintaining the diversity of fauna and flora in Ujung Kulon National Park

The extremely small body size of Williamson’s mouse deer (Tragulus williamsoni) allows coexistence with sympatric larger ungulates through temporal avoidance

Research on the coexistence of congeneric species is essential for understanding community assemblages. Smaller competitors are expected to avoid larger ones, either spatially or temporally, to reduce interspecific competition. According to the spatial scaling law, the greater the difference in body size, the weaker the competitive interactions of the competitors. However, this is not confirmed in the guild of tropical forest ungulates. In this study, we assessed the competitive interactions of Williamson’s mouse deer (Tragulus williamsoni), an endangered species and one of the smallest ungulates in the world, with sympatric larger ungulates. We hypothesized that: 1) because of its extremely small body size, competition with the larger ungulates would be relatively weak, allowing spatial co-existence but still requiring temporal avoidance, and 2) the strength of avoidance would increases with decreasing differences in body size. We set up 238 camera traps from January 2017 to January 2021 to survey Williamson’s mouse deer and the sympatric larger ungulate species, that is, northern red muntjac (Muntiacus vaginalis), wild boar (Sus scrofa), Chinese serow (Capricornis milneedwardsii), and sambar (Rusa unicolor), in the protected areas of Mengla County, southwestern China. We then performed spatio-temporal analyses, including occupancy models, daily activity patterns, and a time interval analysis. Spatially, there was no significant avoidance. Temporally, Williamson’s mouse deer had different daily activity patterns and direct temporal avoidance of all larger ungulate species. The lack of spatial avoidance and strong temporal avoidance supported our first hypothesis, but the stronger avoidance of much larger species ran counter to our second hypothesis. Our results revealed the coexistence mechanism between Williamson’s mouse deer and sympatric larger ungulates and suggested that the difference in body size is limited in explaining the competitive interactions of tropical forest ungulates due to the effects of multiple ecological processes. This deepens our understanding of the relationship between species trait differences and community assembly in tropical forest ecosystems.

Pleistocene Lang Trang fauna in North Vietnam: taxonomic composition and habitats

New data on the taxonomic composition and ecological characteristics of Late Pleistocene vertebrates and invertebrates from the Lang Trang cave in northern Vietnam are presented. Based on the materials collected in progress of the work of the Joint Vietnam - Russia Tropical Science and Technology Research Center in 2020-2022, various representatives of the Stegodon-Ailuropoda Fauna of the second half of the Middle - early Late Pleistocene of Southeast Asia were identified, including primates Gigantopithecus blacki, Pongo sp., Trachypithecus sp., Presbytis sp., Macaca nemestrina, M. cf. fascicularis, M. cf. mulatta, and Macaca sp., carnivorans Panthera tigris, Neofelis nebulosa, and Arctonyx collaris rostratus, a lipotyphlan Chodsigoa hoffmanni, a bat Ia io, rodents Hystrix kiangsenensis, Leopoldamys neilli, and Rattus rattus, proboscideans Stegodon sp. and Elephas maximus, perissodactyls Tapirus indicus, Dicerorhinus sumatrensis, Rhinoceros sondaicus, and Rh. unicornis, artiodactyls Sus scrofa, S. barbatus, Tragulus kanchil, Hydropotes inermis, Muntiacus muntjak, Axis porcinus, Rusa unicolor, Capricornis sumatraensis, Bubalus arnee, and Bos sp., a crocodile Crocodylus sp., a turtle Cuora sp. and an abundant gastropod association, presented by six terrestrial forms, two freshwater species and a coastal-marine species Ellobium aurismidae. Gigantopithecus blacki, Macaca cf. fascicularis, M. cf. mulatta, Neofelis nebulosa, Chodsigoa hoffmanni, Ia io, Leopoldamys neilli, Rattus rattus, Rhinoceros unicornis, Sus barbatus, Tragulus kanchil, Hydropotes inermis, Axis porcinus, crocodiles, turtles, and most of gastropod species were identified for the first time in the Lang Trang Fauna based on these studies. Of particular interest is the finding of the giant ape G. blacki, one of the latest in the fossil record. The habitats of the fauna are characterized as mosaic biotopes with a predominance of wet stations and the participation of mangrove swamps and coastal lowlands; these conditions could have existed in the area of the cave during a major marine transgression of the early Late Pleistocene.

Microtubule search-and-capture model evaluates the effect of chromosomal volume conservation on spindle assembly during mitosis.

Variation in the chromosome numbers can arise from the erroneous mitosis or fusion and fission of chromosomes. While the mitotic errors lead to an increase or decrease in the overall chromosomal substance in the daughter cells, fission and fusion keep this conserved. Variations in chromosome numbers are assumed to be a crucial driver of speciation. For example, the members of the muntjac species are known to have very different karyotypes with the chromosome numbers varying from 2n=70+3B in the brown brocket deer to 2n=46 in the Chinese muntjac and 2n=6/7 in the Indian muntjac. The chromosomal content in the nucleus of these closely related mammals is roughly the same and various chromosome fusion and fission pathways have been suggested as the evolution process of these karyotypes. Similar trends can also be found in lepidoptera and yeast species which show a wide variation of chromosome numbers. The effect of chromosome number variation on the spindle assembly time and accuracy is still not properly addressed. We computationally investigate the effect of conservation of the total chromosomal substance on the spindle assembly during prometaphase. Our results suggest that chromosomal fusion pathways aid the microtubule-driven search and capture of the kinetochore in cells with monocentric chromosomes. We further report a comparative analysis of the site and percentage of amphitelic captures, dependence on cell shape, and position of the kinetochore in respect to chromosomal volume partitioning.

The comparative anatomy of the petrosal bone and bony labyrinth of four small-sized deer.

Here we provide complete 3D reconstructions of the petrosal bone and bony labyrinth of four kinds of small-sized deer (Elaphodus cephalophus, Muntiacus reevesi, Muntiacus muntjak, Hydropotes inermis) based on high-resolution CT scanning, and select one musk deer (Moschus moschiferus) as a comparative object. The petrosal bone and bony labyrinth of E. cephalophus are illustrated for the first time, as well as the petrosal bones of M. reevesi and H. inermis. Some morphological characters of petrosal bone and bony labyrinth can be used to distinguish the above-mentioned species. For example, M. moschiferus shows a prominent transpromontorial sulcus and a ventral basicapsular groove on the petrosal bone; there is a bifurcate cochlear aqueduct on the bony labyrinth of E. cephalophus; there is a distinct fusion between the lateral and posterior semicircular canals on the bony labyrinth of H. inermis. Meanwhile, there are some intraspecific variations on the subarcuate fossa, the tegmen tympani, the cochlear aqueduct, as well as the endolymphatic sac. Our results further confirm that the petrosal bone and bony labyrinth have enormous potential for taxonomy. This work will provide new anatomical data for the phylogenetic study of ruminants in the future, and it will be very practical to identify the isolated ruminants' petrosal bones that are frequently unearthed from paleontological or archeological sites.