Municipal Wastewater Effluent Research Articles

Modelling and optimization of psychoactive pharmaceutical caffeine removal by electrochemical oxidation process: A comparative study between response surface methodology (RSM) and adaptive neuro fuzzy inference system (ANFIS)

In this study, the modelling and optimization of psychoactive pharmaceutical caffeine removal in synthetic solution and real municipal wastewater effluent by electrochemical oxidation (EO) process was investigated using central composite design (CCD) based on response surface methodology (RSM) and adaptive neuro fuzzy inference system (ANFIS). The influence of independent variables including electrolysis time, current intensity, initial concentration of caffeine, and type of anode were studied. Results showed that anode type followed by electrolysis time are the most important variables affecting caffeine degradation. Both CCD and ANFIS models were able to successfully predict the electrochemical process behaviour, while ANFIS models performed slightly better (R2 = 0.993, RMSE = 2.694 for caffeine removal efficiency, and R2 = 0.976, RMSE = 0.261 for energy consumption). Identification of intermediates by liquid chromatography-tandem mass spectrometry (LC-MS/MS) suggests that the degradation of caffeine by the EO process follows an oxidation pathway similar to the mechanism proposed for other advanced oxidation processes. The optimal conditions determined using CCD were applied on real municipal wastewater effluent in which caffeine removal efficiency varied between 78.0 ± 4.3% and 92.5 ± 1.0% for different initial caffeine concentrations showing the effectiveness of the process. Finally, toxicity assessment with Daphnia magna showed that the EO of real municipal wastewater effluent in optimal conditions may increase the toxicity levels of the samples. Toxicity could be reduced by extending the electrolysis time or could be completely eliminated using granular activated carbon.

Sulfite-activated ferrate for water reuse applications

Ferrate is a promising, emerging water treatment technology. However, there has been limited research on the application of ferrate in a water reuse paradigm. Recent literature has shown that ferrate oxidation of target contaminants could be improved by “activation” with the addition of reductants or acid. This study examined the impact of sulfite-activated ferrate in laboratory water matrix and spiked municipal wastewater effluents with the goal of transforming organic contaminants of concern (e.g., 1,4-dioxane) and inactivating pathogenic organisms. Additionally, the formation of brominated disinfection byproducts by activated ferrate were examined and a proposed reaction pathway for byproduct formation is presented. In particular, the relative importance of reaction intermediates is discussed. This represents the first activated ferrate study to examine 1,4-dioxane transformation, disinfection, and brominated byproduct formation. Results presented show that the sub-stoichiometric ([Sulfite]:[Ferrate] = 0.5) activated ferrate treatment approach can oxidize recalcitrant contaminants by >50%, achieve >4-log inactivation of pathogens, and have relatively limited generation of brominated byproducts. However, stoichiometrically excessive ([Sulfite]:[Ferrate] = 4.0) activation showed decreased performance with decreased disinfection and increased risk of by-product formation. In general, our results indicate that sub-stoichiometric sulfite-activated ferrate seems a viable alternative technology for various modes of water reuse treatment.

Fish Exhibit Distinct Fluorochemical and δ15N Isotopic Signatures in the St. Lawrence River Impacted by Municipal Wastewater Effluents

We examined the influence of Montreal wastewater treatment plant (WWTP) effluents on two top predators, Walleye (Sander vitreus) and Sauger (Sander canadensis), with a focus on δ15N isotopic signatures and per- and polyfluoroalkyl substances (PFAS). These two fish species were collected in the summer 2013 in the St. Lawrence River upstream and downstream from a major WWTP, as well as in background sites (semi-remote lakes). Most of the δ15N variations for Sauger and Walleye are attributable to 1) δ15N values of the primary producers and sewage-derived particulate organic matter (SDPOM) at the base of the trophic food chain, 2) agricultural activities combined with biogeochemical processes, and 3) food web length. δ15N was significantly lower in fish collected in the effluent-mixed water masses than other sites of the St. Lawrence River, attributed to the SDPOM of the WWTP effluent. Relative to the background sites, certain PFAS were present at much higher levels in the St. Lawrence River, with profiles dominated by perfluoroalkyl sulfonates (PFSA). However, PFSA profiles generally remained consistent along the St. Lawrence River. PFOS levels in fish from the St. Lawrence exceeded the current Federal Environmental Quality Guidelines for protecting piscivorous mammals or birds. However, the human chronic daily intake of PFOS remained below current thresholds suggested by national agencies.

Enhanced biological wastewater treatment using sodium alginate-immobilized microorganisms in a fluidized bed reactor

In this study, a microbial consortium isolated from an activated sludge tank of a conventional wastewater treatment plant was immobilized using sodium alginate (SA) as a support material for contaminant biodegradation in wastewater. A volume of 500 mL of activated sludge was immobilized in the SA beads (with a mass concentration of 25 g/L). The resulting SA beads were characterized, introduced into a fluidized bed reactor, fed with 1000 mL of the sample, and characterized again after the treatment process. The SA-immobilized microorganisms were tested first for degradation of organic matter (expressed as chemical oxygen demand) and total phosphorous in domestic wastewater, achieving removal efficiencies of 71% and 93%, respectively, after 12 h. Subsequently, the SA-immobilized microorganisms were tested for degradation of a basic blue 9 (BB9) textile dye in a condition that simulated textile wastewater. The efficiency of the BB9 degradation was found to be as high as 99.5% after 2 h. According to these results, SA-immobilized microorganisms were found to be an environmentally friendly and cost-effective alternative for treatment of municipal and industrial wastewater effluents.

Non-target and suspect-screening analyses of hydroponic soybeans and passive samplers exposed to different watershed irrigation sources

Water scarcity increases the likelihood of irrigating food crops with municipal wastewater that may pose potential dietary risks of regulated and non-regulated organic chemical uptake to edible plant tissues. Only a few studies have used high resolution mass spectrometry (HRMS) to assess the uptake of chemicals of concern into food crops. This study used non-target and suspect-screening analyses to compare total chemical features, tentatively identified chemicals (TICs), and EPA ToxCast chemicals in soybean plants and passive samplers exposed to five different irrigation sources that were collected from an agricultural watershed during mild drought conditions. Secondary-treated municipal wastewater effluent, two surface waters, two ground waters, and deionized municipal tap water were used for two hydroponic experiments: soybean roots and shoots and Composite Integrative Passive Samplers (CIPS) harvested after fourteen days of exposure and soybeans after fifty-six days. CIPS were sealed in separate glass amber jars to evaluate their efficacy to mimic chemical features, TICs, and ToxCast chemical uptake in plant roots, shoots, and beans. Total soybean biomass and water use were greatest for tap water, municipal wastewater, and surface water downstream of the municipal wastewater facility relative to groundwater samples and surface water collected upstream of the wastewater facility. ToxCast chemicals were ubiquitous across watershed irrigation sources in abundance, chemical use category, and number. Wastewater-exposed soybeans had the fewest extractable TICs in plant tissues of all irrigation sources. More ToxCast chemicals were identified in CIPS than extracted from irrigation sources by solid phase extraction. ToxCast chemicals in beans and CIPS were similar in number, chemical use category, and log Kow range. CIPS appear to serve as a useful surrogate for ToxCast chemical uptake in beans, the edible food product.

Sex-Specific and Long-Term Impacts of Early-Life Venlafaxine Exposure in Zebrafish.

Simple SummaryExcessive use of antidepressants, combined with our inability to completely clear them from municipal wastewater effluents, has led to their increased presence in aquatic habitats. Venlafaxine, one of the more commonly prescribed antidepressants, has been shown to be detrimental to the early life stages of non-target animals such as fish. Exposure to venlafaxine at the embryonic stage appears to lead to behavioural disruptions when zebrafish become free swimming and reduces growth in juveniles. Here we tested whether early-life exposure also led to behavioural and metabolic perturbations in adults using zebrafish, a widely utilized model in developmental toxicology. Zygotic exposure to venlafaxine compromised activity and anxiety responses and reduced the active metabolic rate as well as the aerobic scope in a sex-specific manner. This study raises the possibility that early developmental exposure to venlafaxine may have long-term consequences on fish performance, and that this may be sex dependent.Venlafaxine, a selective serotonin and norepinephrine reuptake inhibitor, is a widely prescribed antidepressant that is detected in municipal wastewater effluents at µg/L concentrations. It has been shown to impact the early life stages of fish, including neurodevelopment and behaviour in larvae, but whether such early exposures have longer-term consequences are far from clear. Here, we sought to determine whether zygotic deposition of venlafaxine, mimicking a maternal transfer scenario, disturbs the metabolic rate and behavioural performance using zebrafish (Danio rerio). This was tested using freshly fertilized embryos (1–4 cell stage) microinjected with either 0, 1 or 10 ng of venlafaxine and raised to either juvenile (60 days post-fertilization) or adult (10–12 months post-fertilization). Zygotic venlafaxine exposure led to a reduction in the active metabolic rate and aerobic scope, but this was only observed in female fish. On the other hand, the total distance travelled in an open field assessment was greater at the highest concentration of venlafaxine only in the adult males. At the juvenile stage, behavioural assessments demonstrated that venlafaxine exposure may increase boldness—including hyperactivity, lower thigmotaxis, and a reduction in the distance to a novel object. Taken together, these results demonstrate that zygotic venlafaxine exposure may impact developmental programming in a sex-specific manner in fish.

Fate of Dissolved Nitrogen in a Horizontal Levee: Seasonal Fluctuations in Nitrate Removal Processes.

Horizontal levees are a nature-based approach for removing nitrogen from municipal wastewater effluent while simultaneously providing additional benefits, such as flood control. To assess nitrogen removal mechanisms and the efficacy of a horizontal levee, we monitored an experimental system receiving nitrified municipal wastewater effluent for 2 years. Based on mass balances and microbial gene abundance data, we determined that much of the applied nitrogen was most likely removed by heterotrophic denitrifiers that consumed labile organic carbon from decaying plants and added wood chips. Fe(III) and sulfate reduction driven by decay of labile organic carbon also produced Fe(II) sulfide minerals. During winter months, when heterotrophic activity was lower, strong correlations between sulfate release and nitrogen removal suggested that autotrophic denitrifiers oxidized Fe(II) sulfides using nitrate as an electron acceptor. These trends were seasonal, with Fe(II) sulfide minerals formed during summer fueling denitrification during the subsequent winter. Overall, around 30% of gaseous nitrogen losses in the winter were attributable to autotrophic denitrifiers. To predict long-term nitrogen removal, we developed an electron-transfer model that accounted for the production and consumption of electron donors. The model indicated that the labile organic carbon released from wood chips may be capable of supporting nitrogen removal from wastewater effluent for several decades with sulfide minerals, decaying vegetation, and root exudates likely sustaining nitrogen removal over a longer timescale.

Toxicological effects of atenolol and venlafaxine on zebrafish tissues: Bioaccumulation, DNA hypomethylation, and molecular mechanism

The beta-blocker atenolol (ATE), and the selective serotonin and norepinephrine reuptake inhibitor, venlafaxine (VEN) are frequently detected in municipal wastewater effluents, but little is known about their ecotoxicological effect on aquatic animals. Herein, ATE and VEN were selected to explore their accumulation and global DNA methylation (GDM) in zebrafish tissues after a 30-day exposure. Molecular dynamics (MD) stimulation was used to investigate the toxic mechanism of ATE and VEN exposure. The results demonstrated that ATE and VEN could reduce the condition factor of zebrafish. The bioaccumulation capacity for ATE and VEN was in the order of liver > gut > gill > brain and liver > gut > brain > gill, respectively. After a 30-day recovery, ATE and VEN could still be detected in zebrafish tissues when exposure concentrations were ≥10 μg/L. Moreover, ATE and VEN induced global DNA hypomethylation in different tissues with a dose-dependent manner and their main target tissues were liver and brain. When the exposure concentrations of ATE and VEN were increased to 100 μg/L, the global DNA hypomethylation of liver and brain were reduced to 27% and 18%, respectively. In the same tissue exposed to the same concentration, DNA hypomethylation induced by VEN was more serious than that of ATE. After a 30-day recovery, the global DNA hypomethylations caused by the two drugs were still persistent, and the recovery of VEN was slower than that of ATE. The MD simulation results showed that both ATE and VEN could reduce the catalytic activity of DNA Methyltransferase 1 (DNMT1), while the effect of VEN on the 3D conformational changes of the DNMT1 domain was more significant, resulting in a lower DNA methylation rate. The current study shed new light on the toxic mechanism and potential adverse impacts of ATE and VEN on zebrafish, providing essential information to the further ecotoxicological risk assessment of these drugs in the aquatic environment.

COVID-19 and urban rivers: Effects of lockdown period on surface water pollution and quality- A case study of the Zarjoub River, north of Iran.

Due to the spreading of the coronavirus (COVID-19) in Iran, restrictions and lockdown were announced to control the infection. In order to determine the effects of the lockdown period on the status of the water quality and pollution, the concentrations of Al, As, Ba, Cr, Cu, Mo, Ni, Pb, Se, and Zn, together with Na+, Mg2+, Ca2+ and electrical conductivity (EC), were measured in the Zarjoub River, north of Iran, in both pre-lockdown and post-lockdown periods. The results indicated that water pollution and associated human health risk reduced by an average of 30% and 39%, respectively, during the lockdown period. In addition, the multi-purpose water quality index also improved by an average of 34%. However, the water salinity and alkalinity increased during the lockdown period due to the increase of municipal wastewater and the use of disinfectants. The major sources of pollution were identified as weathering, municipal wastewater, industrial and agricultural effluents, solid waste, and vehicular pollution. PCA-MLR receptor model showed that the contribution of mixed sources of weathering and municipal wastewater in water pollution increased from 23 to 50% during the lockdown period. However, the contribution of mixed sources of industrial effluents and solid wastes reduced from 64 to 45%. Likewise, the contribution of traffic-related sources exhibited a reduction from 13% in the pre-lockdown period to 5% together with agricultural effluent in the post-lockdown period. Overall, although the lockdown period resulted in positive impacts on diminishing the level of water pollution caused by industrial and vehicular contaminants, the increase of municipal waste and wastewater is a negative consequence of the lockdown period.Supplementary InformationThe online version contains supplementary material available at 10.1007/s11356-021-18286-5.

The antidepressant venlafaxine perturbs cardiac development and function in larval zebrafish.

Venlafaxine, a selective serotonin and norepinephrine reuptake inhibitor, is a highly prescribed antidepressant and is detected at µg/L concentrations in waterways receiving municipal wastewater effluents. We previously showed that early-life venlafaxine exposure disrupted the normal development of the nervous system and reduces larval activity in zebrafish (Danio rerio). However, it is unclear whether the reduced swimming activity may be associated with impaired cardiac function. Here we tested the hypothesis that zygotic exposure to venlafaxine impacts the development and function of the larval zebrafish heart. Venlafaxine (0, 1 or 10 ng) was administered by microinjection into freshly fertilized zebrafish embryos (1-4 cell stage) to assess heart development and function during early-life stages. Venlafaxine deposition in the zygote led to precocious development of the embryo heart, including the timing of the first heartbeat, increased heart size, and a higher heart rate at 24- and 48-hours post-fertilization (hpf). Also, waterborne exposure to environmental levels of this antidepressant during early development increased the heart rate at 48 hpf of zebrafish larvae mimicking the zygotic deposition. The venlafaxine-induced higher heart rate in the embryos was abolished in the presence of NAN-190, an antagonist of the 5HT1A receptor. Also, heart rate dropped below control levels in the 10 ng, but not 1 ng venlafaxine group at 72 and 96 hpf. An acute stressor reduced the venlafaxine-induced heart rate at 48 hpf but did not affect the already reduced heart rate at 72 and 96 hpf in the 10 ng venlafaxine group. Our results suggest that the higher heart rate in the venlafaxine group may be due to an enhanced serotonin stimulation of the 5HT1A receptor. Taken together, early-life venlafaxine exposure disrupts cardiac development and has the potential to compromise the cardiovascular performance of larval zebrafish.

下水処理水の再利用を目的とした統合的膜処理システムにおけるバイオポリマーの挙動

下水処理水の再利用を目的とした統合的膜処理システムにおけるバイオポリマーの挙動

Genome characterization of the novel lytic genome sequence of the phage YUEEL01 of the Myoviridae family

Antimicrobial resistance is a global concern because of its rapid emergence in the environment and the associated high risk to human and animal health. Municipal wastewater, including urban, hospital, and pharmaceutical effluent, is the primary source of contamination by antibiotics and antibiotic-resistant bacteria (ARB). Biological processes are commonly used for wastewater treatment. Biologically based strategies are a promising approach to effective integrated ARB control because they focus on antibiotic resistance. An effective bacteriophage against multi-drug resistance (MDR) microbes in municipal wastewater was

Impacts of seasonality and operating conditions on algal-dual osmosis membrane system for potable water reuse: Part 2

This study investigated the impact of seasonal variation and operating conditions on recovery of potable quality water from municipal wastewater effluent using an integrated algal treatment process with a dual forward osmosis (FO)-reverse osmosis (RO) membrane system. Pilot study of the algal process treating primary effluent validated the technical viability and seasonal performance during warm weather (May to October, 25–55 °C) using an extremophilic algal strain Galdieria sulphuraria, and during cold weather (November to April, 4–17 °C) using polyculture strains of algae and bacteria. Algal effluents from both seasons were used as the feed solution for the laboratory FO-RO study. In addition, pilot-scale FO-RO experiments were conducted to compare the system performance during treatment of algal effluent and secondary effluent from the conventional treatment facility. At 90% water recovery, the FO-RO achieved over 90% overall rejection of major ions and organic matter using the bench-scale system and over 99% rejection of all contaminants in pilot-scale studies. Detailed water quality analysis indicated that the product water from the integrated system met both the primary and secondary drinking water standards. This study demonstrated that the FO-RO system can be engineered as a viable alternative to treat algal effluent and secondary effluent for potable water reuse independent of seasonal variations and operating conditions.

Mine drainage precipitates attenuate and conceal wastewater-derived phosphate pollution in stream water

Hydrous ferric-oxide (HFO) coatings on streambed sediments may attenuate dissolved phosphate (PO4) concentrations at acidic to neutral pH conditions, limiting phosphorus (P) transport and availability in aquatic ecosystems. Mesh-covered tiles on which “natural” HFO from abandoned mine drainage (AMD) had precipitated were exposed to treated municipal wastewater (MWW) effluent or a mixture of stream water and effluent. Between 42 and 99% of the dissolved P in effluent was removed from the water to a thin coating (~2 μm) of HFO on the mesh. Geochemical equilibrium model results predicted the removal of 76 to 99% of PO4 from the water by adsorption to the HFO, depending on the HFO quantity, initial PO4 concentration, and pH. The measurements and model results indicated the capacity for P removal decreased as the concentration of P associated with the HFO increased. Continuing accumulation of HFO from upstream AMD sources replenish the in-stream capacity for P attenuation below the MWW discharge. This indicates AMD pollution may conceal P inputs and limit the amount of dissolved P transported to downstream ecosystems. However, HFO-rich sediments also represent a potential source of “legacy” P that could confound management practices intended to decrease nutrient and metal loadings.

Statistical modeling of methylene blue degradation by yeast-bacteria consortium; optimization via agro-industrial waste, immobilization and application in real effluents

The progress in industrialization everyday life has led to the continuous entry of several anthropogenic compounds, including dyes, into surrounding ecosystem causing arduous concerns for human health and biosphere. Therefore, microbial degradation of dyes is considered an eco-efficient and cost-competitive alternative to physicochemical approaches. These degradative biosystems mainly depend on the utilization of nutritive co-substrates such as yeast extract peptone in conjunction with glucose. Herein, a synergestic interaction between strains of mixed-culture consortium consisting of Rhodotorula sp., Raoultella planticola; and Staphylococcus xylosus was recruited in methylene blue (MB) degradation using agro-industrial waste as an economic and nutritive co-substrate. Via statistical means such as Plackett–Burman design and central composite design, the impact of significant nutritional parameters on MB degradation was screened and optimized. Predictive modeling denoted that complete degradation of MB was achieved within 72 h at MB (200 mg/L), NaNO3 (0.525 gm/L), molasses (385 μL/L), pH (7.5) and inoculum size (18%). Assessment of degradative enzymes revealed that intracellular NADH-reductase and DCIP-reductase were key enzymes controlling degradation process by 104.52 ± 1.75 and 274.04 ± 3.37 IU/min/mg protein after 72 h of incubation. In addition, azoreductase, tyrosinase, laccase, nitrate reductase, MnP and LiP also contributed significantly to MB degradation process. Physicochemical monitoring analysis, namely UV−Visible spectrophotometry and FTIR of MB before treatment and degradation byproducts indicated deterioration of azo bond and demethylation. Moreover, the non-toxic nature of degradation byproducts was confirmed by phytotoxicity and cytotoxicity assays. Chlorella vulgaris retained its photosynthetic capability (˃ 85%) as estimated from Chlorophyll-a/b contents compared to ˃ 30% of MB-solution. However, the viability of Wi-38 and Vero cells was estimated to be 90.67% and 99.67%, respectively, upon exposure to MB-metabolites. Furthermore, an eminent employment of consortium either freely-suspended or immobilized in plain distilled water and optimized slurry in a bioaugmentation process was implemented to treat MB in artificially-contaminated municipal wastewater and industrial effluent. The results showed a corporative interaction between the consortium examined and co-existing microbiota; reflecting its compatibility and adaptability with different microbial niches in different effluents with various physicochemical contents.

Nanocomposite cation-exchange membranes for wastewater electrodialysis: organic fouling, desalination performance, and toxicity testing

Organic fouling hinders the application of membrane treatment of alternative water. In this study, polyethyleneimine (PEI), titanium dioxide (TiO2) nanoparticles, and graphene oxide (GO) nanosheets were used for modifying cation-exchange membranes to enhance anti-organic fouling and desalination performance during electrodialysis of synthetical water and municipal wastewater. Fouling propensity of the unmodified and modified membranes was quantitatively evaluated by measuring organic accumulation on membrane surface using humic acid (HA, simulating natural organic matter) and bovine serum albumin (BSA, simulating organic matter in municipal wastewater effluent) as model foulants. Significant surface deposition of HA was observed on the unmodified, PEI-TiO2, and PEI-modified membrane surfaces while trivial HA fouling was detected on the PEI-GO membrane. BSA fouling was minor for all the modified membranes compared to the unmodified membrane. Desalination efficiency of electrodialysis with the modified membranes, except the PEI-modified membrane, was significantly improved in treating synthetical water with HA foulant compared to the electrodialysis with the unmodified membrane (20%~40% reduction of normalized salt removal). Relatively stable desalination behaviors were observed for each membrane system in treating BSA-containing synthetical water, and enhanced desalination was demonstrated with the modified membranes. Subsequent long-term fouling experiments with wastewater effluent revealed the superior antifouling capacity of the nanocomposite membranes (especially for the PEI-GO membrane) over 100-hour operation, while the unmodified membranes exhibited severe fouling (with total more than 60% decline of normalized salt removal) after approximately 50-hour treating wastewater. In a standard Microtox® acute toxicity assay, the nanocomposite membranes did not show any acute toxicity towards Vibrio fischeri due to potential leaching of PEI and nanomaterials, indicating safe utilization of the modified membranes for water treatment.

Removal of Specific Pharmaceuticals from Water using Activated Carbon

Many types of pharmaceutical substances have been detected with significant concentrations through various advanced instrumental techniques in surface water, ground water, partially treated water (with concentration typically less than 0,1 ug/L), drinking water (concentration bellow 0,05 ug/L) domestic wastewater, municipal wastewater and industrial effluents (concentration approximately 1 ug/L). Pharmaceutical compounds are found at much lower concentrations in drinking water sources then the normally prescribed doses, but there is concern that chronic exposure to numerous compounds could cause serious health problems and that compounds can act synergistically to cause adverse health effects. The effectiveness of removal the specific pharmaceuticals (paracetamol, carbamazepine, metronidazole and caffeine) from drinking water with adsorption using two types of granular activated carbon (Filtrasorb 400 and WG12) was monitored. Paracetamol is a medication used to treat fever and mild to moderate pain. Paracetamol significantly relieves pain in acute migraine and headache. Carbamazepine is an anticonvulsant medication used primarily in the treatment of epilepsy and neuropathic pain. It is used in schizophrenia along with other medications and as a second-line agent in bipolar disorder. Metronidazole is an antibiotic and antiprotozoal medication. It is used either alone or with other antibiotics to treat inflammatory disease. Caffeine is a central nervous system (CNS) stimulant of the methylxanthine class. It is the world’s most widely consumed psychoactive drug. The concentrations of caffeine in surface waters were detected in the range 0.1 - 6.9 μg/L. The experiments were performed in laboratory conditions with varying values of pH (7,8 or 6,5), stable temperature, and dose of adsorbent. The experiments were performed in the glass bottles with the volume of 400 mL stock model water (drinking water spiked with pharmaceutical standard) with concentration approximately 0.05 μg/L. On the analytical scales weighed out 400 mg GAC was used and then it was added to the bottles. Subsequently these bottles were regularly stirred at 400 rpm. Samples were taken at 30, 60, 120 and 240 minutes, after which they were analyzed. Analyses of target pharmaceutical were performed in laboratories of ALS Czech Republic in Prague. LC-MS methodology (method was validated according to ISO 17025 system) was used to determine pharmaceuticals in water samples. The adsorption efficiency of pharmaceutical removal and the adsorption capacity of granular activated carbon depends on the time of contact of water with the material. Adsorption efficiency for two types of granular activated carbon varies from 13 to more than 90%.

Altered microbiomes of aquatic macroinvertebrates and riparian spiders downstream of municipal wastewater effluents

Municipal wastewater treatment plants (WWTPs) contain numerous contaminants, including antimicrobials, that could affect the composition of the beneficial bacterial communities associated with host aquatic organisms. There is also potential for these effects to transfer to terrestrial predators. Riparian spiders and five families of aquatic macroinvertebrates were collected from sites upstream and downstream of two WWTPs, Waterloo and Kitchener, discharging to the Grand River, Ontario, Canada. Whole-body microbiota were analyzed following the extraction, PCR amplification, and sequencing of bacterial DNA using the V3-V4 hypervariable regions of the 16S rRNA genetic barcode. Changes in the relative abundance of major microbiome phyla were observed in all downstream aquatic insects except Hydropsychidae caddisflies, which exhibited little site variation. Shannon alpha diversity differed among sites for Tetragnathidae spiders, Perlidae, Hydropsychidae, and Heptageniidae. Downstream of the Waterloo WWTP alpha diversity decreased in spiders, while downstream of the Kitchener WWTP this measure decreased in Perlidae and increased in spiders. Bray-Curtis beta diversity was dissimilar among sites in all invertebrate taxa; upstream sites differed from those downstream of Waterloo in spiders, Perlidae, and Hydropsychidae, and from those downstream of Kitchener in spiders, Perlidae, and Hydropsychidae. Finally, effluent-derived bacteria were found in the microbiomes of downstream spiders and aquatic insects and not upstream. Overall, results indicated that the microbiomes of invertebrates collected downstream differed from those collected upstream of WWTPs, which has implications for altered host health and transport of WWTP-derived bacteria through aquatic ecosystems.

Fouling and mitigation mechanisms during direct microfiltration and ultrafiltration of primary wastewater

Direct membrane filtration (DMF) has recently gained attention as an alternative secondary biological wastewater treatment process. This study evaluated direct microfiltration (MF) and ultrafiltration (UF) performance and cleaning protocols during crossflow DMF of primary municipal wastewater effluent. Several types of MF and UF membranes were examined by threshold flux determination, and two types of membranes (MF, 0.08 μm; UF, 100 kDa) were chosen for exploring membrane fouling mechanisms at different feed pressures via both fouling resistance analysis and optical coherence tomography (OCT) observation. The results revealed that both MF and UF displayed three-stage fouling behaviors, i.e., initial intermediate pore blocking followed by two-stage cake filtration. Increasing feed pressure from 8 kPa to 50 kPa could accelerate physically reversible fouling rate (consistent with simulated cake filtration constant). During physical flushing, the cake layer was more readily removed from the UF membrane; while residual porous cake layer was present on the MF membrane, regardless of the feed pressure. With extending filtration-cleaning cycle, shortening filtration duration and elevating cleaning solution temperature to from 25 °C to 50 °C benefited for irreversible fouling alleviation. At 50 °C, the geothermal water performed similar cleaning behaviors as clean water, facilitating lower reversible and irreversible fouling than the geothermal brine. This study shed light on the feasibility of using high temperature geothermal water for periodic physical cleaning during DMF of wastewater under Icelandic scenario.

Dissolved rubidium to strontium ratio as a conservative tracer for wastewater effluent-sourced contaminant inputs near a major urban wastewater treatment plant

Municipal wastewater (MWW) effluent discharges can introduce contaminants to receiving waters which may have adverse impacts on local ecosystems and human health. Conservative chemical constituents specific to the MWW effluent stream can be used to quantify and trace wastewater effluent-sourced contaminant inputs. Gadolinium (Gd), a rare earth element used as a contrasting agent in medical magnetic resonance imaging, can be found in urban MWW streams. Dissolved anthropogenic Gd has been shown to be an indicator and potential conservative tracer for MWW effluent in receiving waters. Like other known MWW tracers, it can be difficult and expensive to measure. Dissolved rubidium (Rb) to strontium (Sr) ratio enrichment in biological materials such as blood and urine can lead to enriched Rb/Sr values in MWW effluent relative to natural waters. This ratio is relatively easy and inexpensive to measure and represents a promising additional indicator for MWW effluent in receiving waters in urbanized freshwater systems. In July 2015 and 2016 surface water samples were collected from sites in the tidal-fresh Potomac River in the vicinity of the Blue Plains Advanced Wastewater Treatment Plant (BPAWWTP) outfall near Washington, DC USA along with treated MWW effluent samples from the BPAWWTP. Dissolved Rb/Sr ratios were measured in these waters and compared to dissolved Gd concentrations in order to demonstrate the potential of the dissolved Rb/Sr ratio as a conservative indicator for MWW effluent. Results suggest the dissolved Rb/Sr ratio represents a simple and cost-effective indicator and conservative tracer for MWW effluent. It can be used with, or in place of, other proven tracers to investigate wastewater impacts in highly-urbanized, anthropogenically-impacted freshwater systems like the tidal fresh Potomac River and perhaps in a wider range of geologic settings than previously thought. A case study is presented as an example to demonstrate the potential of using dissolved Rb/Sr ratios to trace MWW-sourced nutrient inputs from a major WWTP like BPAWWTP to the receiving waters of tidal-fresh Potomac River.