Municipal Abattoir Research Articles

Cystic echinococcosis in cattle slaughtered at a slaughterhouse in Gessa, southern Ethiopia

Cystic echinococcosis is caused by the metacestode of the zoonotic flatworm Echinococcus granulosus. A cross-sectional study was conducted from October 2020 to August 2021 at the Gessa municipality abattoir in southern Ethiopia, through regular meat examinations and cyst characterization to determine the prevalence, organ distribution, and fertility of Cystic echinococcosis. The overall prevalence of Echinococcus granulosus sensu lato was 17.9% (69/384) and has shown a statistically significant association (p < 0.05) with the body condition of cattle but not with other putative risk factors (p > 0.05). The odds of poor body condition cattle infected with CE was 1.29 times higher than medium body condition (OR = 0.506; CI = 0.566–2.96). Based on organ distribution of cysts, the lungs and liver were the most affected organs having a statistically significant difference (p < 0.05) with the prevalence of CE. Based on the size of cysts, small (47.9%) size cysts revealed the highest prevalence as compared with large (33.3%) and medium (18.8%) sized cysts. On cyst characterization, the majority of Cystic echinococcosis were sterile cysts (55.4%) followed by fertile (38.8%) and calcified (8.7%) cysts and revealed a statistically significant difference (p < 0.05). Cyst number, organ localization, fertility of cyst, and cyst size have shown a strong positive correlation (r = 0.8686, r = 0.8393, r = 0.9506, and r = 0.9189) with the prevalence of CE, respectively. The presence of an overall high prevalence of Echinococcus granulosus sensu lato in the present study required urgent action to interrupt the life cycle of Cystic echinococcosis and further studies using molecular techniques to characterize the circulating genotypes to prevent the risk of zoonosis in humans in the study area.

In Vitro Anthelmintic Activity and Phytochemical Screening of Crude Extracts of Three Medicinal Plants against Haemonchus Contortus in Sheep at Haramaya Municipal Abattoir, Eastern Hararghe.

The current study was carried out to evaluate the anthelmintic efficacy of crude methanolic extracts of leaves of Croton macrostachyus and Nicotiana tabacum and rhizome parts of Zingiber officinale on nematode parasite, Haemonchus contortus. For these objectives, adult worm mortality assay (AWMA) was conducted on adult H. contortus to investigate the in vitro adulticidal effect of crude extracts from March to October 2021 G.C. The percentage yield of crude 99.9% methanolic extracts was 53%, 78%, and 44% for C. macrostachyus, N. tabacum, and Z. officinale, respectively. Phytochemical analysis using standard techniques was also used to detect secondary metabolites contained in the plants. The study revealed the presence of secondary metabolites such as tannins, flavonoids, steroids, and terpenoids in all extracts, which are considered to be the chemical components that are responsible for the wide therapeutic activities of several medicinal plants. In in vitro study, four graded concentrations of the crude extracts (500 mg/ml, 250 mg/ml, 125 mg/ml, and 62.5 mg/ml) were tested at regular time intervals, and parasite viability for 8 hours was recorded in triplicate. Albendazole (1.25 mg/ml) and distilled water were used as the positive and negative controls, respectively. At 4 hr posttreatment, the 62.5 mg/ml, 125 mg/ml, and 250 mg/ml concentrations of C. macrostachyus, N. tabacum, and Z. officinale extracts have caused significantly higher mortality (P < 0.05) compared to the albendazole. Methanolic extracts of C. macrostachyus, N. tabacum, and Z. officinale produced mortality of adult H. contortus significantly (P < 0.05) to the level of 93%, 83%, and 50% at concentration of 125 mg/ml at 4 hr posttreatment and meanwhile at 6 hr produced 100%, 100%, and 90%, respectively, at the same concentration. On the other hand, albendazole (1.25 mg/ml) killed 60% and 80% of the parasites at 4 hr and 6 hr posttreatment, respectively. Concentrations of all the extracts had showed a comparable and strong nematocidal effect on H. contortus having no significant difference with that of the positive control (P > 0.05) at 8 hr posttreatment period. Hence, the current study revealed that the extracts from three plants have potential anthelmintic effect, and we recommend further study on fractionating each component separately and validating the materials using other parasite developmental stages are warranted.

Prevalence and Public Health importance of Bovine Cysticercosis in Haramaya Municipal Abattoir, East Hararghe Zone of Oromia Regional State, Eastern Ethiopia

Aim This study was conducted by using the protocols of post-mortem examinations of meat (visual inspection) to determine the prevalence of bovine cysticercosis in the cattle slaughtered at Haramaya Municipal Abattoir, Eastern Hararghe, Ethiopia and to determine zoonotic significance of taeniasis. Method A cross-section study design was conducted from November 2020 to March 2021, based on routine meat inspection on simple randomly selected cattle slaughtered at the abattoir. Visual inspection of all exposed surface was made in all active organs. They are shoulder muscles, hearts, masseters (cheek muscle), diaphragms, tongues and livers. This is followed by incision of all those organs to be examined for Cysticercus bovis cysts. Results Twenty-one (21) of the 384 cattle examined utilizing the post-mortem examinations meat inspection methodology were positive for C. bovis, resulting in a prevalence of 5.5 %. The masseter muscle (11.5 %) had the highest prevalence of cysts, followed by the triceps (8.3%), heart (5.8%), liver (4.8 %), and tongue (2.9 %). The sex-based prevalence rates were 10 (3.4%) and 11 (12.1%), respectively. The predominance of bodily condition was found to be good (1.3%), medium (11.1%), and bad (50 %). The prevalence male and female e differed substantially by organ, sex, and bodily condition (p&gt;0.05), but not statistically significant by age of the animals (young 2.8% vs. adult 6.1%) (p&gt;0.05). Eight (20%) of the total 40 interviewees had contracted Taenia saginata infection at least once in their lives. Religion showed a significant difference (p&gt;0.05) (Christian 66.7% and Muslim 6.5%). However, there was no statistically significant difference in meat consumption habits (raw 31.6% vs. cooked 9.5%), sex (male 26.5% vs. female 11.8%), age (young 33.3% vs. adult 17.7%), educational status (illiterate 22.2% vs. elementary 14.3% vs. high school 27.3% vs. college 16.7%) or latrine use (proper users 19.4% and non-proper users 25%). Conclusion This study to increasing public awareness of the disease, as well as strict routine meat inspections, should be prioritized in order to decrease the parasite's impact.

Prevalence of Cryptosporidium infection in slaughtered cattle at Lafenwa Municipal Abattoir, Ogun State

The prevalence of Cryptosporidium in slaughtered cattle at a municipal abattoir in Ogun State was determined by acid-fast staining of stools. Using formalin ethyl acetate sedimentation followed by modified kinyoun’s acid fast staining of 280 faecal samples collected, 105 (37.5%) were positive for Cryptosporidium oocysts. Infection was higher in females and nondiarrhoeic cattle than males and diarrhoeic animals respectively. However, there were no statistical differences (p&gt;0.05) between the sexes and stool consistencies in Cryptosporidium infection. The highest infection rate, 51.2%, was recorded in the Red Bororo breed while the lowest rate, 17.0%, was recorded in the Sokoto Gudali breed. This study showed that cattle slaughtered at the abattoir were mostly infected with Cryptosporidium which may have serious economic, veterinary, and public health implications to beef consumers, abattoir, and farm workers within the state.

Study on prevalence of indigestible foreign bodies in rumen and reticulum of cattle slaughtered at Bedeno Woreda Municipal Abattoir, Eastern Ethiopia

From October 2020 to April 2021, a cross-sectional study was undertaken at Bedeno Woreda Municipal Abattoir in Eastern Ethiopia with the goal of identifying and characterizing the prevalence of cattle indigestible rumen and reticulum foreign body, as well as its risk variables. This study included 384 cattle that were chosen at random. Prior to postmortem examination, an ante mortem examination was used to characterize a specific animal. A total of 154 (40.1%) of the 384 cattle evaluated were found to have indigestible foreign materials in their rumen and reticulum. Female cattle (54.4 percent) had a substantially greater prevalence of foreign bodies (2 = 25.817, p 0.05) than male cattle (29.0 percent). In terms of breed, the prevalence of foreign bodies was higher in cross breed (56.2 percent) animals than in local breed animals (38.6 percent). Poor (71.2 percent) had substantially more foreign bodies collected (2 = 69.138, p 0.05) than medium (52.9) and good (52.9). Score for bodily condition (18). The results showed that age has a significant connection (2 = 40.626, p 0.05) among different age groups of animals for foreign bodies (21.4 percent in young, 40.8 percent in adults, and 70.3 percent in old). The rumen was shown to have a higher percentage of foreign bodies (78.0%) than the reticulum (10.1%), with the remaining percent present in both the rumen and the reticulum. Plastic (53.4%) was the most commonly found foreign material in the rumen, while metallic material (35.3%) was found in the reticulum. The findings of this study revealed that improper solid waste garbage disposal in habitats poses a considerable danger of animal and environmental damage. As a result, the responsible government and community should focus their efforts on preventing the problem from worsening.

Status of small ruminant fasciolosis and diagnostic test evaluation at Haramaya town municipal abattoir, East Harrarghe Zone, Ethiopia

A cross-sectional study to estimate the prevalence of fasciolosis and associated risk factors at Haramaya Municipal Abattoir was conducted from November 2017 to April 2018. Moreover, the direct financial loss due to liver condemnationand evaluation of the sensitivity of direct sedimentation technique for detection of Fasciola eggs were performed. A total of 384 small ruminants comprising of 235 sheep and 149 goats were subjected to coprological and postmortem examination. An overall prevalence of 12.8% was found. Forty-four animals were positive for eggs of Fasciola with an overall prevalence of 11.5%. However, it was 14.1% (54/384) in the examination of the same animals exposed to postmortem examinations of fluke-infected livers. The prevalence of fasciolosis based on body condition score was 62.2%, 12.4%, and 4.1% in that order, in poor, medium, and good body condition groups and the difference was statistically significant (p&lt;0.05). Other factors considered like, the monthly prevalence, species, age, and sex of the animals did not show significant differences (p&gt;0.05). The prevalence of fasciolosis was highest in Haramaya (19.0%)followed by Awaday (14.9%), Kersa (10.3%), Midhaga (8.1%), and the lowest prevalence was observed in Babile (5.8%), however, the difference in prevalence across the origin of the animals was not significant (p &gt; 0.05). F. hepatica was a dominant 63.0 % (34/54) species identified followed by F. gigantica 20.4% (11/54) and 5.6% (3/54) and 11.1% (6/54) were due to mixed and immature flukes, respectively. Taking liver examination as the gold standard for diagnosisof fasciolosis, the sensitivity of the direct sedimentation technique was found to be 81.5% and the specificity 100% with almost perfect agreement (k =0.89) between the two methods. The direct financial loss due to liver condemnation was estimated to be 46,318.5 (965 USD) Ethiopian birr per annum. In conclusion, Fasciola spp are important parasites affecting small ruminants in the study area. Control strategies targeted to the parasite and the intermediate hosts (snail) with particular emphasis on mature poorly conditionedanimals is recommended.

Prevalence of Bovine Cysticercosis in Jigjiga City Municipal Abattoir and Treatment Costs of Human Taeniasis

Combination of Cross-sectional and retrospective study was conducted from Jan 2021 up to Jun 2021 in Jigjiga city Municipal abattoir and inventory of pharmaceutical drug shops, with the objectives of determining the prevalence of bovine cysticercosis and treatment costs of human taeniasis. Systematic random sampling technique was employed for selecting the study animals, while the Sampling methodology was based on Active abattoir survey and Inventory of pharmaceutical drug shops. From the 520 animals examined at abattoir, the overall prevalence of cysticercus bovis was 2.69% (14/520) with 95% CI of (1.43, 4.29). There was no statistically significant difference in sex, age, body condition score and origin of the animal with the occurrence of C. bovis (P&gt;0.05); From 14 infected cattle with cysticercus bovis, 7 (50%) of cysticercus bovis were in the triceps muscle, 5 (35.71%) in tongue, 1 (7.14%) in biceps muscle and 1 (7.14%) in masseter muscle. Descriptive statistics revealed that a total of 23,063 doses of taenicidal drugs concern a 4-year period (2017-2020) were used for the treatment of human taeniasis which worth 56,933 Ethiopian Birr in the town. Albendazole was employed at a rather high dose, followed by Mebendazole, Praziquantel, and Niclosomide, in that order. The most commonly prescribed medicines for the treatment of taeniasis were albendazole and mebendazole. The discovery highlights the importance of cysticercosis and taeniasis in terms of both economics and public health. To reduce the impact of this parasite, special focus should be paid to raising public awareness and conducting thorough routine meat inspections.

Occupational safety and health hazards associated with the slaughtering and meat processing industry in urban areas of Zimbabwe: A case study of the Gweru city Municipal Abattoir

This study examined occupational safety and health hazards associated with the slaughtering and meat processing industry in the Gweru City Municipal abattoir in Zimbabwe. Qualitative and quantitative research designs were employed. The study population involved all the 23 workers including flayers and labourers who are directly involved in slaughtering and meat processing. Data was collected with questionnaire administration, interviews and field observations. Safety and health hazards associated with slaughtering and meat processing at the abattoir included physical, biological, ergonomic, mechanical, environmental and food safety hazards, all with the risk of causing significant harm to the workers. The lack of experience on job operations was one of the key risk factors increasing the likelihood of workers getting injured and increased frequency of injuries. Workers who had more working years at the abattoir encountered injuries less frequently than those who had fewer working years. The abattoir’s occupational safety and health management system was not effective in solving safety and health risks and required improvement. One effective way that can be implemented is to establish a stand-alone safety, health and environment department within the Gweru City Council to give priority to occupational safety and health issues. Training programmes for workers and management on safety and health are recommended to improve workers’ skills on job tasks, hazard identification, and risk assessment and to improve workers’ behaviour as well as to enhance awareness on safety and health among management, which ultimately improves management commitments.

Epidemiological status of bovine cysticercosis and human taeniasis in Eastern Ethiopia

Bovine cysticercosis and human taeniasis are among the leading cause of economic loss in Ethiopia due to organ condemnation and treatment costs. A cross-sectional study was conducted from September 2017 to July 2018 on randomly selected carcasses from Jigjiga, Babile and Dire Dawa town municipal abattoirs to estimate the prevalence of bovine cysticercosis. Besides, a questionnaire was administered to the human population of these towns to understand risk of human taeniasis. The overall prevalence of Cysticercus bovis was 27.3% (302/1108). Among the examined predilection sites, the highest prevalence was observed in the liver (9.6%), and the tongue (8.5%). From the total of 686C. bovis cysts collected, 289 (42.0%) were viable, while the other 397 (58.0%) were non-viable cysts. Three predictors, namely study location, age and body condition, were significantly associated with C. bovis (p ≤ 0.001). Among the 900 respondents interviewed, 432 had contracted Taenia saginata infection. Risk factors like occupation, sex, marital status, educational status and raw beef consumption habit were significantly associated with T. saginata infection (p ≤ 0.001). The findings of this study indicated the importance of bovine cysticercosis and taeniasis in the study areas. Therefore, attention should be given to public awareness and detailed meat inspection for the safety of the public and promotion of the country's meat industry.

Prevalence, Risk Factors, and Diagnostic Efficacy of Bovine Tuberculosis in Slaughtered Animals at the Chiang Mai Municipal Abattoir, Thailand.

This study aimed to (1) investigate the prevalence of bovine tuberculosis (bTB) in slaughtered animals at the Chiang Mai Municipal abattoir in Chiang Mai, Thailand; (2) identify animal-level risk factors for bTB at the abattoir; and (3) evaluate the performance of techniques for bTB detection at the abattoir. From April 2020 to March 2021, 161 animals registered for slaughter were randomly selected for the study. Animal data including age, sex, species, body condition scores, and origins of the animals were collected. Meat inspection was performed by a trained meat inspector. Tissue samples of the lung, liver, and lymph nodes were collected for histopathological diagnosis and polymerase chain reaction (PCR) detection of Mycobacteria and specifically Mycobacterium bovis. The prevalence of bTB during meat inspection and PCR was calculated separately. Animal-level factors affecting bTB were determined using multivariate logistic regression analysis. The performance of meat inspection and PCR was evaluated using a Bayesian approach. The prevalence of bTB was 12.4% (20/161) and 34.8% (56/161) when the disease was diagnosed using meat inspection and PCR, respectively. Buffaloes had a significantly higher risk of being identified as bTB-positive using PCR compared to beef cattle (odds ratio = 2.19; confidence interval = 1.11–4.30). The median of posterior estimates of sensitivity (Se) and specificity (Sp) to detect bTB using meat inspection were 20.8% [95% posterior probability interval (PPI) = 9.1–36.5%] and 87.8% (95% PPI = 79.6–95.4%), respectively. The medians of the posterior estimates of Se and Sp for PCR were 88.6% (95% PPI = 70.5–98.3%) and 94.4% (95% PPI = 84.7–98.8%), respectively. These findings demonstrate that bTB is highly prevalent among slaughtered animals. PCR can be used as an ancillary test for bTB surveillance at abattoirs in Thailand.

Bovine Trypanosomiasis Prevalence and Associated Risk Factors at the Ngaoundere Municipal Abattoir

Bovine Trypanosomiasis Prevalence and Associated Risk Factors at the Ngaoundere Municipal Abattoir

Prevalence of Foreign in Rumen and Reticulum of Cattle Slaughtered at Bedele Municipal Abattoir

A Cross sectional study was conducted on 384 slaugtered cattle at Bedele municipal abattoir to determine the prevalence of foreign bodies in their rumen and reticulum and to identify the common risk factors associated with their occurrence. Rumen and reticulum were thoroughly examined by visual inspection and palpation and all the contents were inspected. The types of foreign bodies were identified after washing. From a total of 384 animals examined, 52 (13.5) were found positive for the presence of foreign bodies in rumen and no foreign body was found in reticulum. The types of foreign bodies detected were piece of cloth, plastics, rope and shoelace. Piece of cloth were the most common with a prevalence of 10.2%. Prevalence of foreign bodies were significantly associated with ade and body condition of the animals (p&lt;0.05). Foreign bodies were more frequent in older cattle (&gt;8years) than in younger (4-8years). The prevalence of ruminal foreign bodies were highest in poor body conditioned animals compared to cattle with better body condition. The prevalence of foreign body ingestion found in cattle by this study show that littering the environment with piece of cloth and other indigestible materials could pose health problem for free grazing cattle.

Laboratory analysis as support to slaughterhouse inspection in Songea cattle abattoir (Tanzania): A public health perspective.

Livestock breeding is one of the main agricultural activities in Tanzania, recognized as one of the African countries with the greatest livestock resources. Cattle is the most widely bred animal species, especially with traditional farming methods such as pastoralism and agro-pastoralism. Slaughtering takes place mainly in rural slaughter slabs and municipal slaughterhouses, and according to local legislation it must be supervised by Official Veterinarians, who report any organs to be discarded and, after stamping the carcass, authorize its free consumption. Since the Ruvuma region lacks data on cattle diseases, the aim of this study was to collect information at Songea slaughterhouse, with particular attention to conditions of parasitic aetiology and potential zoonotic interest, deepening inspective diagnoses by means of parasitological and histological investigations on tissue samples. Overall, the slaughter data of 614 animals are reported. In most cases slaughtered cattle belonged to local breeds and came from the Mbeya region. Fasciolosis (diagnosed in 44.6% of animals) was the most frequent condition, followed by respiratory diseases (24.4%), aortic onchocerciasis (6.5%), hydatidosis (5.5%) and bovine cysticercosis (3.4%). Laboratory analysis identified Fasciola gigantica as the species involved in hepatic distomatosis in this area. Cases macroscopically ascribed to cysticercosis turned out to be a muscular form of onchocerciasis (by Onchocerca dukei), a neglected and unrecognised parasitic disease in Tanzania, questioning the real frequence of a zoonosis that causes primarily major economic losses. Pulmonary cysts, referred as hydatidosis at visual inspection, were histologically identified as haemal nodes, lymphoid organs common in ruminants, but described in cattle lungs for the first time in the present paper. Slaughterhouse is an important epidemiological observatory, especially for neglected parasitoses. The possibility of having basic laboratory diagnostics as an aid to visual inspection can ensure greater efficiency of veterinary services in the control of important livestock diseases and zoonoses, in the frame of a One Health perspective.

Determination of Tetracycline Residues in Kidney and Muscle of Beef Cattle Slaughtered in Dire Dawa and Harar Municipal Abattoirs, Eastern Ethiopia.

Background:Antibiotics are among the most widely used drugs in farm animals for therapeutic and prophylactic purposes. However, the antibiotic residues in meat are a serious public health concern due to their harmful effects on consumer health. Besides this problem, there is limited information on the level of the antibiotic residues, including tetracycline residues. Therefore, this study was aimed to determine the tetracycline residues in the kidney and muscle samples of beef cattle in Harar town and Dire Dawa city.Methods:A study was conducted on 500 randomly selected carcass (250 kidney and 250 muscle samples) slaughtered at Dire Dawa and Harar municipal slaughterhouses between December 2018 and December 2019. The samples were collected aseptically and screened for tetracycline residues by thin layer chromatography. Then, presumptive positive samples were analyzed using high-performance liquid chromatography to get a quantitative outcome. Descriptive statistics were used to determine the frequency, mean, or standard deviation to determine the summary values and distribution of the outcomes. Finally, the data was analyzed using SPSS version 21 software.Results:Out of 500 samples, oxytetracycline residues were detected in 84% of the samples. However, tetracycline and doxycycline were not detected in all samples. Among the kidney and muscle samples collected from Dire Dawa and Harar abattoirs, 109 (87.2%) and 101 (80.8%) were positive for oxytetracycline, respectively. Oxytetracycline residue levels in Dire Dawa ranged from 57 to 607 µg/kg for the kidney and 10.14 to 435 µg/kg for muscle samples. Among the samples collected from Harar, the concentration of oxytetracycline residues ranged from 16 to 433 µg/kg and 6 to 435 µg/kg for kidney and muscle samples, respectively, at Harar slaughterhouses. About 22.0% of muscle samples collected from Dire Dawa and 17.8% from Harar town had oxytetracycline residues above maximum residue limits.Conclusions:In general, the study revealed that oxytetracycline residues were prevalent among tetracycline residues analyzed from kidney and muscle samples in the study areas. Thus, there is a risk of consumer exposure to these antibiotic residues that may have human health effects. Therefore, awareness creation and strict regulation is needed by the regulatory authorities for the use of antimicrobial drugs in the livestock industry.

Prevalence, Related Risk Factors and Distribution of Cyst of Cysticercus bovis in Cattle Subjected to Slaughter at Nekemte Municipal Abattoir, Western Ethiopia

Prevalence, Related Risk Factors and Distribution of Cyst of Cysticercus bovis in Cattle Subjected to Slaughter at Nekemte Municipal Abattoir, Western Ethiopia

Prevalence, Associated Risk Factors and Cyst Distribution of Hydatidosis in Cattle Slaughtered at Nekemte Municipal Abattoir, Western Ethiopia

Prevalence, Associated Risk Factors and Cyst Distribution of Hydatidosis in Cattle Slaughtered at Nekemte Municipal Abattoir, Western Ethiopia

Assessment of Hygienic Status of Some Local Abattoirs and its Impact on Meat Quality

The current study was conducted to evaluate the hygienic status of some local abattoirs and its impact on meat quality through determination of aerobic plate, Enterobacteriaceae, coliforms, Staphylococcus aureus, Enterococci, molds, and yeasts counts. This study was conducted in 5 municipal slaughterhouses located in different provinces, Egypt. A total of 300 samples were collected as following: slaughtered meat, water, and air samples beside swabs from floor, wall, and workers hand (50 samples/each). Statistical analytical results of aerobic plate count clarified that the highest mean value was recorded in the floor swabs (8.9×105 CFU/g) followed by hand swabs of workers (7.41×105 CFU/g) then wall swabs and air samples (7.2×105 CFU/g) then water samples (5.2×105 CFU/g) and lastly meat samples (1.3×105 CFU/g). The highest mean value of Enterobacteriaceae count was recorded in the floor swabs (3.2×104 CFU/g) followed by wall swabs (9.3×103 CFU/g) then air samples (6.63×103 CFU/g) then hand swabs of workers (5.33×103 CFU/g) then water samples (3.19×103 CFU/g) and lastly meat samples (9.02×102 CFU/g). The highest mean value of coliforms count was recorded in the floor swabs (1.65×103 CFU/g) followed by hand swabs of workers (7.23×102 CFU/g) then meat samples (6.10×102 CFU/g) then water samples (4.10×102 CFU/g) then wall swabs (1.72×102 CFU/g) and lastly air samples (1.32×102 CFU/g). On comparison of various microbiological counts on the investigated meat samples with the Egyptian standards, it was clear that most of samples failed to comply with standards confirming the role of contaminated abattoir environment in the obtained result. Results of microbiological examination of the collected samples reflected a clear state of contamination in abattoirs environment that would affect the microbiological quality of the produced meat from these abattoirs that in turn would be harmful for human health.

Copper and Zinc Levels in the Liver Tissue of Healthy Cattle Affected by Fasciola hepatica in a Slaughterhouse

Fasciolosis is considered the most important liver disease in animals, the most notorious damages are caused by death, it causes a reduction in the production of meat, wool and milk, seizure of affected organs, secondary infections by bacteria, interference with fertility and expenses derived from its treatment. The objective of this research was to determine the relationship of Cu and Zn levels in the liver with the affectation by Fasciola hepatica in slaughterhouse cattle. Determinations of Cu, Zn, ash and dry matter were carried out using the atomic absorption spectrophotometry technique in healthy liver tissue affected by fasciolosis in 40 cattle slaughtered in the slaughterhouse. The association between possible risk factors for hepatic Cu and Zn values below the critical limit and the event of livers affected by F. hepatica was carried out in a retrospective case-control observational study. The levels of Cu and Zn in healthy livers affected by F. hepatica in cattle slaughtered in the municipal slaughterhouse of Sagua la Grande, Villa Clara, Cuba are deficient and show low reserves of these microelements with values below the critical value. No association was found between Cu and Zn levels in liver tissue with liver involvement by F. hepatica in slaughterhouse cattle, although it was observed that slaughtered cattle with hepatic Zinc values lower than the critical limit are affected approximately two times more due to fasciolosis than bovines with normal hepatic zinc values.

Seroprevalence of leptospirosis among slaughtered cattle in Thrissur, Kerala#

Leptospirosis is anunderdiagnosed bacterial anthropozoonotic disease that is considered as a major public health concern in India. The present study was conducted to detect the presence of antibodies against different leptospiral serovars in slaughtered cattle in Thrissur. A total of 165 blood samples were collected from slaughtered cattle of two slaughterhouses viz., Meat Technology Unit (MTU), KVASU, Mannuthy (n=85) and Thrissur Municipal Slaughterhouse (TMS), Thrissur (n=80). The serum samples from the slaughtered cattle were subjected to Microscopic Agglutination Test (MAT). The overall occurrence of leptospirosis from both the slaughterhouses was 44.42 per cent. A seropositivity of 37.65 and 51.25 per cent in slaughtered cattle respectively were found by MAT from samples collected from MTU, Mannuthy and TMS, Thrissur. The predominant serovars detected in slaughtered cattle were Sejroe (28.76 per cent), Grippotyphosa (21.91 per cent) and Australis (15.06 per cent).The results revealed that the apparently healthy slaughtered cattle had been exposed to leptospirosis and could act as a source of infection to the slaughterhouse workers.

Study on the Prevalence and Estimation of Monetary Loss of Hydatidosis in Cattle Slaughtered at Bedeno Municipal Abattoir, East Hararghe, Ethiopia

Aim This study was conducted by using the protocols of standard meat inspection (visual, palpation and incision) to assess the prevalence and associated risk factors and to estimate the economic loss in cattle slaughtered at Bedeno Municipal Abattoir, Eastern Hararghe, Ethiopia. Method A cross-section study design was conducted from February 2020 to November 2020, to determine the prevalence and its associated risk factors of bovine hydatidosis is taking animal origin, sex and age and body condition as risk factors. Results From A total of 355 cattle examined using the protocol of standard meat inspection, 99(27.8%) of them are found positive to hydatid cysts. The prevalence of cattle hydatidosis based on animal origin was highest in older cattle 89(29.47%) than young 10(18.89%). Regarding body condition, score to significance variation was observed, as the prevalence was 41.89% for poor cattle followed by medium 24.07% and good 24.37%. Of the total 99 infected, 41(11.55%) of animals were infected in their lungs with only hydatid cysts and 24(6.76%) were infected in their liver, but the rest animals had multiple organ infections, 34(9.58%). Considering the current result, the total annual economic loss from organ condemnation and carcass weight loss due to bovine hydatidosis at Bedeno Municipal Abattoir was estimated at 6,671,803.8 Ethiopian birr (ETB). Conclusion This study reveals that cattle hydatidosis was a high prevalence diseases in the study area and needs paucity of information on control and prevention. The cattle owners to be educated and awareness programmes to be conducted about the diseases.