Mung Bean Seeds Research Articles

Optimization of The Priming Time of Mannitol Solution for Mungbean Seed Treatment

Abstract not available Bangladesh Agron. J. 2024, 26(2): 100-103

Effects of Purified Vitexin and Iso-Vitexin from Mung Bean Seed Coat on Antihyperglycemic Activity and Gut Microbiota in Overweight Individuals' Modulation.

Exceeding a healthy weight significantly elevates the likelihood of developing type 2 diabetes (T2DM). A commercially available singular constituent, available as either purified vitexin or iso-vitexin, has been associated with a decreased risk of T2DM, but its synergistic effect has not been reported yet. Vitexin and iso-vitexin were extracted using an ethanol-based solvent from mung bean seed coat (MBCE) and subsequently purified using preparative liquid chromatography (Prep-LC). Eleven mixture ratios of vitexin and/or iso-vitexin were determined for their antioxidant and antihyperglycemic activities. The 1:1.5 ratio of vitexin to iso-vitexin from MBCE demonstrated the most synergistic effects for enzyme inhibition and glucose uptake in HepG2 cells within an insulin-resistant system, while these ratios exhibited a significantly lower antioxidant capacity than that of each individual component. In a gut model system, the ratio of 1:1.5 (vitexin and iso-vitexin) regulated the gut microbiota composition in overweight individuals by decreasing the growth of Enterobacteriaceae and Enterococcaceae, while increasing in Ruminococcaceae and Lachnospiraceae. The application of vitexin/iso-vitexin for 24 h fermentation enhanced a high variety of abundances of 21 genera resulting in five genera of Parabacteroides, Ruminococcus, Roseburia, Enterocloster, and Peptacetobacter, which belonged to the phylum Firmicutes, exhibiting high abundant changes of more than 5%. Only two genera of Proteus and Butyricicoccus belonging to Proteobacteria and Firmicutes decreased. The findings suggest that these phytochemicals interactions could have synergistic effects in regulating glycemia, through changes in antihyperglycemic activity and in the gut microbiota in overweight individuals. This optimal ratio can be utilized by industries to formulate more potent functional ingredients for functional foods and to create nutraceutical supplements aimed at reducing the risk of T2DM in overweight individuals.

Regenerated cellulose films with controllable microporous structure for enhanced seed germination

In this work, the preparation of high-performance and porous regenerated cellulose (RCNH) films for seed germination application were investigated. The films were prepared from bamboo-based cellulose carbamate-NaOH/ZnO/urea and coagulated using environmentally friendly aqueous solution of (NH4)2SO4. The results showed that the pore size of the films could be efficiently controlled by changing the concentration and temperature of the coagulation bath. In a mild environment, the system remains undisturbed, resulting in slow diffusion between the solvent and coagulation bath. This allows for the cellulose molecular chains to align in parallel and self-aggregate, forming a three-dimensional network structure. Therefore, the best mechanical properties were demonstrated by a film coagulated using 5 wt% (NH4)2SO4 solution at 10 °C. This film showed excellent tensile strength of 108 MPa and high elongation at break (35 %). As compared to a plastic wrap, the film demonstrated higher permeability for oxygen, and a moisture retaining ability. Due to these properties, it could be used as an agricultural film to encase and promote the growth of mung bean seeds. Moreover, the film was biodegradable with a short decomposition time, losing 90.75 % of its original mass after 63 days. In a summary, this work provides a route for robust, biodegradable, and permeable regenerated cellulose films with potential applications as biodegradable agricultural mulches.

Evaluation Of Mung Bean (Vigna Radiata) Varieties For Resistance To Bruchid (Callosobruchus Spp) Infestation Using The “No Choice Test"

Mung beans (Vigna radiata) commonly known as green grams, are an important legume crop cultivated for food and as a source of income. Mung bean seeds are highly susceptible to infestation by bruchids (Callosobruchus spp), which can cause significant post-harvest losses, hence impacting on the global food security. Bruchid infestation control remains a priority, and overreliance on chemical pesticides contributes to environmental concerns due to the potential harm to non-target species such as soil and water quality. Investigating natural resistance mechanisms in mung beans can contribute to sustainable and eco-friendly pest management practices. This study aimed at identifying mung bean varieties resistant to storage bruchids using the 'No Choice' test by determining the seed damage. Twenty-three mung bean varieties, both wild and local, were obtained from KALRO Katumani and evaluated against pulse beetle (Callosobruchus maculatus) under laboratory conditions. Fifty seeds of each test sample were placed in separate petri dishes. Five male and female pairs of 0–24-hour-old adults of the beetle were released into each petri dish, covered to prevent insect escape and allow air circulation. The observations were made after 72 hours on oviposition preference by determining the number of eggs laid and percentage seed damage by counting the seeds with one or more holes from the total. The mung bean variety V100-35226 exhibited the highest resistance to bruchid infestations, with minimal seed damage. Other varieties with slight resistance included AMVU-1612, AMVU-1601, AMVU-1603, and V100-1802. The results highlighted significant differences in resistance levels among the mung bean varieties, indicating that some varieties possess natural resistance mechanisms to bruchid infestation

Inactivation of Salmonella Typhimurium on mung bean seeds by combined application of microbubbles and disinfections and its effect on sprouting

Microbial contamination is the major safety concern of sprouts, and its main source is the seeds. Achieving effective reduction requires very high concentrations of chlorine-based disinfectants (>10,000 mg/kg). Thus, the objective of this study was to combine microbubble (MB) with chlorine dioxide (ClO2, 500 mg/L) and slightly acidic electrolyzed water (SAEW, containing 250 mg/L of available chlorine) to inactivate Salmonella Typhimurium on mung bean seeds. MB water containing disinfectants (10 L) was generated for 10 min, then the seeds were treated for 10, 20, or 30 min. Significant reductions were obtained by combining MB with disinfectants. After 20-min treatment, ClO2-MB (ClMB) achieved additional reductions of 3.7 and 2.3 log CFU/g, respectively, compared with water washing and ClO2 only. SAEW-MB (SMB) achieved additional reductions of 3.6 and 2.0 CFU/g, respectively when compared with water washing and SAEW only after 20-min treatment. No significant difference for the reduction in S. Typhimurium was observed (p ≥ 0.05) between 20-min and 30-min treatments, but increased damage to seeds was evident after 30-min treatment. More surface cracks were observed on the seeds treated with ClMB than on those subjected to water washing and ClO2 only when examined under a scanning electron microscope. However, the germination rate of seeds and the weight increase of sprouts were not significantly different between treatments of ClMB, water washing, and ClO2 only. A large-volume MB device (100 L) was established, resulting in a reduction of S. Typhimurium and natural microflora by 3.1 and 3.3 log CFU/g, respectively, after a 20-min ClMB treatment. This study's findings demonstrated how MB enhances the effectiveness of disinfectants and its potential capacity for large-scale operation.

Transformative impact of atmospheric cold plasma on mung bean seeds: Unveiling surface characteristics, physicochemical alterations, and enhanced germination potential

This work investigates the impact of a new geometry Atmospheric Cold-Plasma (ACP) system on mung bean (Vigna radiata) seed germination and seedling growth. Plasma is produced using a bipolar pulse power source, providing superior control of the generated species. A substantial increase in germination rates (86.67%–91.67%) and optimal seedling growth after 40 s of treatment is reported. The functional and morphological features have shown improvements. Plasma treatment significantly increases the specific surface area (7.695 m2/g) and total pore volume (0.007 cc/g) of the seeds compared to the control (2.214 m2/g and 0.001 cc/g, respectively), suggesting potential enhancements in water absorption and nutrient exchange. Radicle growth stimulation is also observed without alterations in the seed’s chemical structure. These findings highlight the potential of the ACP system for enhancing mung bean sprout germination and seedling development, which is helpful for agriculture applications.

Potential of Aqueous Extracts From Root Organs of Ratoon Plants Sorghum Cultivated on Inceptisols Soil as Bioherbicide

Purpose: This study aims to determine the best inhibitory effect of water extracts from the ratoon root organs of sorghum plants grown on Inceptisol soil on the germination of test plants. Methodology: The study used a single-factor completely randomized design with four treatments: control, Numbu roots, Super 1 roots, and Suri 4 roots. The experiment units were petri dishes, and the experiment was repeated four times. The bioassay method in petri dishes was applied, with 10 ml of water extract added to each dish, and 25 Vima 1 mung bean seeds were incubated for three days. Results: The water extracts from the ratoon roots of Numbu and Suri 4, as well as all other sorghum ratoon root extracts, significantly inhibited the germination of test plants compared to the control. Findings: Sorghum ratoon root extracts exhibit potent bioherbicidal properties. Novelty: This research provides insights into the allelopathic potential of different sorghum varieties. Originality: The study offers a detailed analysis of the bioherbicidal effects of sorghum ratoon root extracts on seed germination. Conclusions: Water extracts from sorghum ratoon roots, especially Numbu and Suri 4, effectively inhibit test plant germination. Type of Paper: Empirical Research Article

Mechanistic insights into the plant biostimulant activity of a novel formulation based on rice husk nanobiosilica embedded in a seed coating alginate film.

Seed coating ensures the targeted delivery of various compounds from the early stages of development to increase crop quality and yield. Silicon and alginate are known to have plant biostimulant effects. Rice husk (RH) is a significant source of biosilica. In this study, we coated mung bean seeds with an alginate-glycerol-sorbitol (AGS) film with embedded biogenic nanosilica (SiNPs) from RH, with significant plant biostimulant activity. After dilute acid hydrolysis of ground RH in a temperature-controlled hermetic reactor, the resulting RH substrate was neutralized and calcined at 650°C. The structural and compositional characteristics of the native RH, the intermediate substrate, and SiNPs, as well as the release of soluble Si from SiNPs, were investigated. The film for seed coating was optimized using a mixture design with three factors. The physiological properties were assessed in the absence and the presence of 50 mM salt added from the beginning. The main parameters investigated were the growth, development, metabolic activity, reactive oxygen species (ROS) metabolism, and the Si content of seedlings. The results evidenced a homogeneous AGS film formation embedding 50-nm amorphous SiNPs having Si-O-Si and Si-OH bonds, 0.347 cm3/g CPV (cumulative pore volume), and 240 m2/g SSA (specific surface area). The coating film has remarkable properties of enhancing the metabolic, proton pump activities and ROS scavenging of mung seedlings under salt stress. The study shows that the RH biogenic SiNPs can be efficiently applied, together with the optimized, beneficial alginate-based film, as plant biostimulants that alleviate saline stress from the first stages of plant development.

Insights from Morpho-Physiological Traits Imparting Tolerance for Preharvest Sprouting of Mungbean Genotypes

Due to the absence of fresh seed dormancy (FSD) in mung bean seeds makes them susceptible to pre-harvest sprouting (PHS). Mung bean yield has been proved to be 60–70% reduced in response to PHS, an abiotic stress. By understanding this problem present study was carried out at at experimental field, Department of Agricultural Botany (Plant Physiology) (20.70060N, 77.03710E), PGI, Dr. Panjabrao Deshmukh Krishi Vidyapeeth, Akola during kharif (rainy) season 2012-13. The experiment entitled “Insights from morpho-physiological traits imparting tolerance for preharvest sprouting of munbean genotypes”. The design of experiment is Randomized block design in three replications with sixteen genotypes. It was observed that on the basis of morpho-physiological traits viz., high hard seed %, pod pubescence, lower germination in pod and seed, less seed and pod moisture genotype AKM-9801, AKM-9907, BM-2002-1 and AKM-10-05, genotypes were found to be tolerant to preharvest sprouting. And more interesting fact is the seed yield per hector found efficient in AKM-8802 and AKM-9907. So, it was found that AKM 9907 is best suitable variety of mungbean in kharif season to prevent crop from whether damage and improve yield also.

Transcriptomics and starch biosynthesis analysis in leaves and developing seeds of mung bean provide a basis for genetic engineering of starch composition and seed quality.

Mung bean starch is distinguished by its exceptional high amylose content and regulation of starch biosynthesis in leaves and storage tissues, such as seeds, share considerable similarities. Genetic engineering of starch composition and content, requires detailed knowledge of starch biosynthetic gene expression and enzymatic regulation. In this study we applied detailed transcriptomic analyses to unravel the global differential gene expression patterns in mung bean leaves and in seeds during various stages of development. The objective was to identify candidate genes and regulatory mechanisms that may enable generation of desirable seed qualities through the use of genetic engineering. Notable differences in gene expression, in particular low expression of the Protein Targeting to Starch (PTST), starch synthase (SS) 3, and starch branching enzyme1 (SBE1) encoding genes in developing seeds as compared to leaves were evident. These differences were related to starch molecular structures and granule morphologies. Specifically, the starch molecular size distribution at different stages of seed development correlated with the starch biosynthesis gene expression of the SBE1, SS1, granule-bound starch synthases (GBSS) and isoamylase 1 (ISA1) encoding genes. Furthermore, putative hormonal and redox controlled regulation were observed, which may be explained by abscisic acid (ABA) and indole-3-acetic acid (IAA) induced signal transduction, and redox regulation of ferredoxins and thioredoxins, respectively. The morphology of starch granules in leaves and developing seeds were clearly distinguishable and could be correlated to differential expression of SS1. Here, we present a first comprehensive transcriptomic dataset of developing mung bean seeds, and combined these findings may enable generation of genetic engineering strategies of for example starch biosynthetic genes for increasing starch levels in seeds and constitute a valuable toolkit for improving mung bean seed quality.

Composites of sodium alginate based - Functional materials towards sustainable adsorption of benzene phenol derivatives - Bisphenol A/Triclosan

The present study evaluates the adsorption efficiency of low-cost carbonaceous adsorbents as fly ash (FA), saw dust biochar (SDB) (untreated and alkali - treated), live/dead pulverized white rot fungus Hypocrea lixii biomass encapsulated in sodium alginate (SA) against the commercially available activated carbon (AC) and graphene oxide (GO) SA beads for removal of benzene phenol derivatives - Bisphenol A (BPA)/triclosan (TCS). Amongst bi - and tri - composites SA beads, tri-composite beads comprising of untreated flyash - dead fungal biomass - sodium alginate (UFA - DB - SA) showed at par results with commercial composite beads. The tri - composite beads with point zero charge (Ppzc) of 6.2 was characterized using FTIR, XRD, surface area BET and SEM-EDX. The batch adsorption using tri - composite beads revealed removal of 93% BPA with adsorption capacity of 16.6 mg/g (pH 6) and 83.72% TCS with adsorption capacity of 14.23 mg/g (pH 5), respectively at 50 ppm initial concentration with 6 % adsorbent dose in 5 h. Freundlich isotherm favoring multilayered adsorption provided a better fit with r2 of 0.9674 for BPA and 0.9605 for TCS respectively. Intraparticle diffusion model showed adsorption of BPA/TCS molecules to follow pseudo - second order kinetics with boundary layer diffusion governed by first step of fast adsorption and intraparticle diffusion within pores by second slow adsorption step. Thermodynamic parameters (ΔH°, ΔS°, ΔG°) revealed adsorption process as exothermic, orderly and spontaneous. Methanol showed better desorbing efficiency leading to five cycles reusability. The phytotoxicity assay revealed increased germination rate of mung bean (Vigna radiata) seeds, sprinkled with post adsorbed treated water (0 h, 5 h and 7 h) initially spiked with 50 ppm BPA/TCS. Overall, UFA - DB - SA tri - composite beads provides a cost effective and eco - friendly matrix for effective removal of hydrophobic recalcitrant compounds.

Effect of pomegranate peel on physicochemical and antioxidant properties of tortilla chips prepared from germinated corn and mung bean flour

There is increasing demand of innovative functional foods in the market to cure widely prevalent deficiencies observed globally. In line with this issue, the study was aiming to replace existing snacking habits with the healthier ones by using corn and mung bean seeds to produce their respective germinated flours for production of tortilla chips with varied concentrations (5 %, 10 %, 15 %) of pomegranate peel powder (PPP) for enhancing the shelf life of the chips without use of synthetic preservatives. All these variations were then fried at three temperatures 170, 180, 190ºC for different times 75 s, 60 s, 45 s, respectively and were then evaluated for their proximate composition, antioxidant properties, sensory properties and shelf life for a period of 40 days. Ash content analysis showed increased content up to 7.6 % for T"1 (with 15 % PPP/170 °C). Total antioxidant activity and phenolic content increased with increased peel concentration and decreased with increased frying temperature. After control, the maximum overall acceptability among samples having peel powder was of T2 (with 5 % PPP and fried at 180 °C) with score 7.68. However, the peroxide value and free fatty acid value were found minimum for T"3 (with 15 % PPP and fried at 190 °C) indicating an extended shelf life.

Freeze-Thaw Stability Regulating Mechanism of Polysaccharide Extracted from Mung Bean Seed Coat on Rice Starch Gel: Retardation of Retrogradation and Ice Crystal Growth

Freeze-Thaw Stability Regulating Mechanism of Polysaccharide Extracted from Mung Bean Seed Coat on Rice Starch Gel: Retardation of Retrogradation and Ice Crystal Growth

Analyzing the Mycoflora Associated with the Mungbean (Vigna radiata L.) Seeds under Various Storage Circumstances

Mungbean (Vigna radiata L.) is a popular pulse consumed throughout the world. It has a balanced nutritional profile containing considerable levels of bioactive compounds, proteins, dietary fiber, minerals and vitamins. As a result, it becomes important to produce and store it. The primary goal of this study is to determine the losses caused by seed storage and to select appropriate alternatives that will minimize those losses. To determine the optimal solution through this investigation, a variety of containers have been employed to preserve seeds against mycoflora. This study looked at the test weight of the seeds, their ability to germinate, and the fungus that was present. The major finding of this investigation under various storage conditions (using ten containers for storage) the percentage of 1000 seed weight was maximum in gunny bags (10.76%) while the lowest weight was gained in glass pots (5.25%). The germination percentage of seed under different storage conditions revealed that maximum was observed in Glass pot (94.69%) over 120 days while, minimum of gunny bag (91.31%) and the germination percentage gradually decreased from 30 DAS to 120 DAS. A. flavus, A. niger, Rhizopus spp., Penicillium spp. under storage condition increased from 30 DAS to 120 DAS while Alternaria spp., Macrophomina spp., Fusarium spp. and Trichoderma spp. gradually decreased under different periods of the interval of storage.

Solusi Recycle Sampah Organik dan Anorganik bagi Keluarga di Perumahan Taman Dramaga Permai 2 Bogor

Households are one of the contributors to organic and inorganic waste. Generally, the family needs to manage waste independently. The concept of waste recycling can be applied at the household level. The aim of waste management in the Dosen Mengabdi Reguler 2022 was to provide alternative solutions for processing organic and inorganic waste in the household based on the recycling concept. The activities were carried out from July‒November 2022 in one of the villages around the IPB campus locations, namely the Taman Darmaga Permai 2, Cihideung Ilir, Ciampea, Bogor. The core activities consist of discussions and demonstrations about compost, coenzymes, re-growing vegetable plants, and the culture of mung bean sprouts from Vima 1 and Vimil 1 varieties. About 42.9% of the 14 participants sorted household waste, and 28.6% managed waste. Compost from organic waste was harvested within one month after the process, while coenzymes were harvested after three months. Ecoenzymes were attractive to all participants as a solution for processing waste. About 50% of participants favor growing vegetables and 21.4% re-grow vegetable waste. Katuk and Welsh onion plants have 100% growth and 90% survival rates. All participants had never sprouted mung bean seeds. The sprouts can be harvested 1 to 3 days after sowing as needed. The Vima 1 mung bean variety has a sweet sprout taste and produces 657.9 g of sprouts from 100 g of seeds, while the Vimil 1 taste is slightly astringent with a lower sprout production of 543.8 g.

Waste-liquors generated during Handmade paper manufacture from cow dung as a potential source of biofertilizer

In India, cow dung is widely utilized to create formulations that serve as effective plant growth enhancers with antimicrobial properties. The cow dung-derived handmade paper manufacturing process produces two waste-liquor streams, a Raw Liquor (RL) produced through the dewatering of the cow-dung slurry and a Black Liquor (BL) produced during the soda pulping of dewatered cow dung. The present study explored the potential of these waste streams to be used as plant biofertilizers for germination and cultivation of Vigna radiata seeds (mung bean, IPM-02-03 variety). An in vitro assay for seed germination efficiency and a pot study for plant growth promotion (PGP) activity were used to assess this potential. The in vitro assay demonstrated that nutrient-rich RL, with its 100% seed germination efficiency (better than the 85.7% of tap water) was an effective biofertilizer for seed germination. In contrast, BL yielded poor seed germination efficiency. The pot study showed that water irrigation led to good seed germination, survival, plant rooting and shooting, but it was probably deprived of nutrients for inducing good grain yields. When water was replaced with RL, it was able to replicate the results, but with good grain yields. In contrast, BL produced poorer germination, seed survival and PGP results. The poor biofertilization efficiency of BL was most probably due to the nutrient losses and toxic chemicals produced in the harsh pulping process. The results showed that RL, though considered a waste stream, is sufficiently nutrient-rich to act as an effective biofertilizer for germinating mung bean seeds and promoting plant growth and grain yields.

Drying kinetics and mass transfer parameters of mung beans dried using a convective dryer

Convective drying of mung bean seeds was conducted to investigate drying kinetics and mass transfer parameters at temperatures of 40, 50, 60, 70, and 80°C and a fixed air velocity of 1.2 m s-1. Drying characteristics were determined by drying rate, moisture ratio, diffusion coefficient, activation energy, Biot number, and mass transfer coefficient. Drying models such as Newton, Page, Henderson and Pabis, Logarithmic, Midilli, Two-term, and Diffusion approximation were applied and fitted to the moisture ratio of the experimental data. The drying rate was significantly affected by the drying temperature. The Midilli model was considered the most suitable for experimental drying curves. The effective moisture diffusivity ranged from 3.19×10-9 to 10.76×10-9 m2 s-1, with an activation energy of 59.78 kJ mol-1. Similar to the mass transfer coefficient, the Biot number increased with increasing drying temperature. The simultaneous transfer of heat and mass was controlled by diffusion and surface.

Controlled solvothermal synthesis of self-assembled SrTiO3 microstructures for expeditious solar-driven photocatalysis dye effluents degradation

In this work, the self-assembled SrTiO3 (STO) microstructures were synthesized via a facile one-step solvothermal method. As the solvothermal temperature increased from 140 °C to 200 °C, the STO changed from a flower-like architecture to finally an irregularly aggregated flake-like morphology. The photocatalytic performance of as-synthesized samples was assessed through the degradation of rhodamine B (RhB) and malachite green (MG) under simulated solar irradiation. The results indicated that the photocatalytic performance of STO samples depended on their morphology, in which the hierarchical flower-like STO synthesized at 160 °C demonstrated the highest photoactivities. The photocatalytic enhancement of STO-160 was benefited from its large surface area and mesoporous configuration, hence facilitating the presence of more reactive species and accelerating the charge separation. Moreover, the real-world practicality of STO-160 photocatalysis was examined via the real printed ink wastewater-containing RhB and MG treatment. The phytotoxicity analyses demonstrated that the photocatalytically treated wastewater increased the germination of mung bean seeds, and the good reusability of synthesized STO-160 in photodegradation reaction also promoted its application in practical scenarios. This work highlights the promising potential of tailored STO microstructures for effective environmental remediation applications.

Activation of peroxymonosulfate with natural pyrite-biochar composite for sulfamethoxazole degradation in soil: Organic matter effects and free radical conversion

Peroxymonosulfate (PMS)-based advanced oxidation processes (AOPs) represent an effective method for the remediation of antibiotic-contaminated soils. In this study, a natural pyrite-biochar composite material (FBCx) was developed, demonstrating superior activation performance and achieving a 76% removal rate of SMX from soil within 120 min. There existed different degradation mechanisms for SMX in aqueous and soil solutions, respectively. The production of 1O2 and inherent active species produced by soil slurry played an important role in the degradation process. The combination of electron paramagnetic resonance (EPR) and free radical probe experiments confirmed the presence of free radical transformation processes in soil. Wherein, the·OH and SO4·− generated in soil slurry did not directly involve in the degradation process, but rather preferentially reacted with soil organic matter (SOM) to form alkyl-like radicals (R·), thereby maintaining a high concentration of reactive species in the system. Furthermore, germination and growth promotion of mung bean seeds observed in the toxicity test indicated the environmental compatibility of this remediation method. This study revealed the influence mechanism of SOM in the remediation process of contaminated soil comprehensively, which possessed enormous potential for application in practical environments.

Transport of nZVI/copper synthesized by green tea extract in Cr(IV)-contaminated soil: modeling study and reduced toxicity.

In this study, nano-zero-valent iron/copper was synthesized by green tea extracts (GT-nZVI/Cu) and produced a stable suspension than nano-zero-valent iron synthesized by green tea extracts (GT-nZVI) injected into Cr(VI)-containing soil column. The equilibrium 1D-CDE model was successfully used to fit the penetration curves of Fe(tot), Fe(aq), and Fe(0) in order to determine the relevant parameters. The hydrodynamic dispersion coefficient of chromium-contaminated soil was 0.401 cm2·h-1, and the pore flow rate was 0.144cm·h-1. The stable C/C0 of Fe(tot), Fe(aq), and Fe(0) in the effluent were retarded to 0.39, 0.79, and 0.11, respectively, compared to a ratio of 1 for the concentration of the tracer Cl- in the effluent to the concentration in the influent. Additionally, the 1D-CDE model describes the migration behavior of Cr(VI) with a high R2 (> 0.97). The obtained blocking coefficients declined gradually with increasing concentration of GT-nZVI/Cu suspension and decreasing concentration of Cr(VI). The content of reduced chromium in the soil decreased from 2.986 to 1.121 after remediation, while the content of more stable oxidizable chromium and residual chromium increased from 2.975 and 20.021 to 16.471 and 27.612. The phytotoxicity test showed that mung bean seeds still had a germination rate of 90% (control of 100%), root length of 29.63mm (control of 35.25mm), and stem length of 17.9cm (control of 18.96cm) after remediation with GT-nZVI/Cu. These indicated that GT-nZVI/Cu was effective in immobilizing Cr(VI) in the soil column and reduced the ecological threat. This study provides an analytical basis and theoretical model for the migration of chromium-contaminated soil in practical application.