The charging pump of a nuclear power plant is a multi-stage double shell centrifugal pump. From the installation period, the pump shaft broke after 25 times of start-up and shutdown and 2913 hours of cumulative operation. As the charging pump is one of the core equipment in the normal operation and accident of the nuclear power plant, the root cause of the pump shaft fracture must be found and corrective measures must be taken, otherwise there will be major safety hazards. Through the analysis of the material, design structure and mechanical properties of the pump shaft, it is found that the root cause is that there is a deviation in the processing of the structural dimension of the stress relief groove of the pump shaft, which leads to the insufficient fatigue strength margin and the occurrence of abnormal operation conditions during the superposition commissioning, which leads to the fracture of the pump shaft. By optimizing the structural design of the stress relief groove and controlling the reasonable operation, no fracture occurred again. The causes and treatment of this typical event can provide certain theoretical support for the design, operation and maintenance of similar equipment.