Multi-spectral imaging by unoccupied aerial vehicles provides a non-destructive, high throughput approach to measuring biomass accumulation over successive alfalfa (Medicago sativa L. subsp. sativa) harvests. Information from estimated growth curves can be used to infer harvest biomass and to gain insights into the relationship between growth dynamics and forage biomass stability across cuttings and years. In this study, multi-spectral imaging and several common vegetation indices were used to estimate genetic parameters and model growth of alfalfa cultivars to determine the longitudinal relationship between vegetation indices and forage biomass. Results showed moderate heritability for vegetation indices, with median plot level heritability ranging from 0.11-0.64, across multiple cuttings in three trials planted in Ithaca, NY, and Las Cruces, NM. Genetic correlations between the normalized difference vegetation index and forage biomass were moderate to high across trials, cuttings, and the timing of multi-spectral image capture. To evaluate the relationship between growth parameters and forage biomass stability across cuttings and environmental conditions, random regression modeling approaches were used to estimate the growth parameters of cultivars for each cutting and the variance in growth was compared to the variance in genetic estimates of forage biomass yield across cuttings. These analyses revealed high correspondence between stability in growth parameters and stability of forage yield. The results of this study indicate that vegetation indices are effective at modeling genetic components of biomass accumulation, presenting opportunities for more efficient screening of cultivars and new longitudinal modeling approaches that can provide insights into temporal factors influencing cultivar stability.