Forest structure spatially varies at the landscape-scale (between-plot) but also local-scale (within-plot). Both scales of structural variability have significant impacts on forest ecosystem functioning and stability. However, we still have a limited understanding of how tree species richness affects the spatial structural variability. In this study, we disentangled within- and between-plot variability in stand structural variables depending on tree species richness level. Terrestrial laser scanning (TLS) technology, capable of developing forest vertical structure in 3D detail, was applied in the FORBIO tree diversity experiment at three sites in Belgium. One site was 9-year old (Hechtel-Eksel) and other two sites were 11-year old (Zedelgem and Gedinne). Each site included five different sets of one, two, three, and four tree species mixtures with one replica. At each plot, four single scan locations were used. Six stand structural variables were quantified based on TLS vertical plant profiles. We hypothesized that within-plot structural variability would increase with species richness, but that the between-plot structural variability would decrease with species richness. Our study revealed notable differences in structural variability between monocultures and mixtures across all sites examined. However, the relationship between tree species richness and structural variability patterns is site-dependent. Specifically, at the Zedelgem site, we observed increased within-plot variability and decreased between-plot variability with species richness, supporting the hypothesis that species richness drives stand structural variability. However, the hypothesized pattern was not as strong at the Hechtel-Eksel and Gedinne sites. Moreover, the various structural variables displayed contrasting responses to changes in species richness. These findings indicated the potential influence of tree species richness on within- and between-plot structural variability in young plantations, particularly in favorable site conditions with well-adapted species pools. Hence, afforestation measures would benefit from planting multiple tree species to initiate structurally complex stands by considering species richness level.
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