Multipath diversity leads to a possible higher performance for real-time high definition video, especially for medical video transmission, which would improve the stability of multiple transmission paths in the symmetrical state, and avoid the potential losses of imaging information in the communication process. Most of the previous works are always based on the single-path end-to-end transmission, although the service had been demonstrated that it is unable to meet the rigorous demand for the RT-HDMV. In the paper, a multipath relay service based on the QoE (quality of experience) evaluation method is proposed for the RT-HDMV (real-time high definition medical video). The method eliminates several of the limitations in the existing methods for some conventional single-path transmission. It can fully utilize the finite network resources and transmission bandwidth to meet the users’ demands of the RT-HDMV to get a better score of the QoE. We use a four-stage framework to evaluate the QoE, which consists of constructing the multipath relay transmission for the RT-HDMV, calculating the weights of diversified QoS parameters in the multipath, designing the load distribution strategy by the mapping between the QoS (quality of service) and QoE, and redefining the rule of the QoE evaluation. Many experiments show that the proposed design scheme achieves weighting of the transmission sub-paths and computes the QoE score. Compared with the state-of-art methods in the single path transmission scene, our framework mainly gains the excellent performance for the RT-HDMV.