Multiple Stress Research Articles

PSII-20 Effect of increasing glycine equivalent in low crude protein diets on growth performance in broiler chickens raised under multiple stress conditions

Abstract The objective of the current experiment was to investigate the effects of increasing glycine (Gly) equivalent in low crude protein (LCP) diets on growth performance in broiler chickens raised under multiple stress (MS) conditions. Ross 308 broiler chickens (n = 450; 22 d of age) were randomly allotted to 1 of 6 treatments with 6 replicates in a 2 × 3 factorial arrangement. The first factor included two different environmental conditions (normal condition vs. MS condition) and the second factor included three dietary treatments (CON, control diets; LCP, low crude protein diets; LCP+Gly, LCP diets with Gly supplementation). Birds in normal conditions were raised under recommended environmental conditions (22 ± 0.9°C and 53.6 ± 5.99 %) with low stock density (SD; 16.9 birds/m2). Birds in MS conditions were exposed to a cyclic heat stress condition (32 ± 0.1°C for 8 h/d and 26°C ± 0.3°C for the remaining time) with high SD (25.3 birds/m2). The average relative humidity was 37.9 ± 4.94%. In addition, 15 birds per cage were assigned in MS conditions to increase SD, whereas 10 birds per cage were assigned in normal conditions. The treatment diets included CON diets with adequate energy, crude protein (19.50% CP and 1.15% Gly equivalents), and other nutrients recommended for growing broiler chickens and LCP diets with low CP content (16.50% CP and 1.15% Gly equivalents) but adequate other nutrients. The LCP+Gly diets had increasing concentrations of Gly equivalents (17.19% CP and 1.73% Gly equivalents) by Gly supplementation to LCP diets. The diets and water were provided ad libitum throughout the experiment. Experiment lasted for 16 d from 22 d to 37 d of age. At the conclusion of the study, growth performance including body weight gain (BWG), feed intake (FI), and feed conversion ratio (FCR) was determined. All data were analyzed by 2-way ANOVA in a completely randomized design using the PROC MIXED procedure of SAS (SAS Institute Inc., Cary, NC). Results indicated that no interactions between environmental conditions and dietary treatments were observed for all growth performance measurements (Table 1). For the main effects, as expected, MS conditions decreased (P < 0.001) growth performance of broiler chickens, regardless of dietary treatments. The LCP diets increased (P < 0.05) FI and FCR as compared with CON diets, regardless of environmental conditions. However, LCP+Gly diets decreased FI (P < 0.05) and tended to decrease FCR (P = 0.076) as compared with LCP diets. In conclusion, either MS conditions or LCP diets impair growth performance in broiler chickens with no significant interaction. However, increasing Gly equivalents by Gly supplementation to LCP diets are likely to improve feed efficiency in broiler chickens regardless of stress environments.

Functional characterization of MiFTs implicated in early flowering and stress resistances of mango

Phosphatidylethanolamine binding protein (PEBP) family plays important roles in multiple developmental processes in plants. In this study, a total of 11 PEBP gene family members were identified from the mango (Mangifera indica L.) genome, and these proteins were divided into three subfamilies based on their phylogenetic relationships: TERMINAL FLOWER 1 (TFL1)-like, MOTHER OF FT AND TFL (MFT)-like, and FLOWERING LOCUS T (FT)-like. Expression analysis revealed that MiFT1a, MiFT1b and MiFT2 were expressed mainly in leaves, whereas MiFT3 and MiFT4 were expressed mainly in embryos. The overexpression of MiFTs significantly promoted early flowering under both long- and short-day conditions. Interestingly, it still significantly promoted early flowering at 16 °C and 28 °C, with MiFT1a exhibiting the most significant, followed by MiFT1b and MiFT2. Additionally, the expression level of MiFT3 is related to the embryonic development of mango. Further studies revealed that overexpression of MiFT3 inhibited seed germination in transgenic Arabidopsis lines. In addition, the MiFT1a and MiFT1b transgenic lines did not respond to abiotic stress, while MiFT2, MiFT3 and MiFT4 enhanced resistance to salt or drought stress in Arabidopsis. Yeast two-hybrid (Y2H) and bimolecular fluorescence complementation (BiFC) assays revealed that MiFTs can interact with flower related and multiple stress proteins, such as bZIP protein (MiFD), 14–3-3 protein, zinc finger protein (MiZFP4), RING zinc-finger protein (MiRZFP34), and phosphatase 2C (MiPP2C25A and MiPP2C25B). These results indicate that FT subfamily not only regulates flowering but also participates in stress response, but there are differences in the function among these genes.

A comparative study into the effect of spent coffee powder and ash on improving the mechanical properties of bitumen

Nowadays, waste and renewable materials are used to modify bitumen and asphalt, reduce costs, decrease the use of natural resources, and maintain ecological balance, and have thus received much attention. This study aimed to improve the rheological properties of bitumen and reduce spent coffee grounds' (SCG) waste by partially replacing it with SCG powder and ash. SCG powder and ash were added in percentages of 0, 1, 3, 5, and 8 wt percent of bitumen to check the properties of control and modified bitumens. Microstructural properties were evaluated by Fourier transform infrared spectroscopy, thermal gravimetric analysis, X-ray fluorescence spectroscopy (XRF), and scanning electron microscopy (SEM). Penetration, softening point, and ductility tests were performed to check the physical properties of bitumens, along with the storage stability test. Rheological tests (viscosity, bending beam rheometer (BBR), dynamic shear rheometer (DSR), multiple stress creep and recovery (MSCR), and linear amplitude sweep (LAS) were also carried out. According to the BBR results, adding SCG powder up to −12 °C and adding SCG ash up to −6 °C increased the resistance to low temperatures. Based on DSR and MSCR results, SCG, either in the form of ash or powder, increased the resistance to rutting, and adding SCG ash improved the high temperature performance of bitumen by 1 grade. At 64 °C, adding 8 % of SCG powder increased the rutting resistance by 43 %, and adding SCG ash raised the rutting resistance by about 113 %. LAS results also showed that SCG ash outperforms SCG powder in fatigue. Although both SCG powder and ash performed well in high, moderate, and low temperatures, SCG powder performed better in cold regions, and SCG ash performed better in regions with temperate and tropical climates.

Genome-Wide Identification and Characterization of Alternative Oxidase (AOX) Genes in Foxtail Millet (Setaria italica): Insights into Their Abiotic Stress Response.

Alternative oxidase (AOX) serves as a critical terminal oxidase within the plant respiratory pathway, playing a significant role in cellular responses to various stresses. Foxtail millet (Setaria italica), a crop extensively cultivated across Asia, is renowned for its remarkable tolerance to abiotic stresses and minimal requirement for fertilizer. In this study, we conducted a comprehensive genome-wide identification of AOX genes in foxtail millet genome, discovering a total of five SiAOX genes. Phylogenetic analysis categorized these SiAOX members into two subgroups. Prediction of cis-elements within the promoter regions, coupled with co-expression network analysis, intimated that SiAOX proteins are likely involved in the plant's adaptive response to abiotic stresses. Employing RNA sequencing (RNA-seq) and real-time quantitative PCR (RT-qPCR), we scrutinized the expression patterns of the SiAOX genes across a variety of tissues and under multiple abiotic stress conditions. Specifically, our analysis uncovered that SiAOX1, SiAOX2, SiAOX4, and SiAOX5 display distinct tissue-specific expression profiles. Furthermore, SiAOX2, SiAOX3, SiAOX4, and SiAOX5 exhibit responsive expression patterns under abiotic stress conditions, with significant differences in expression levels observed between the shoot and root tissues of foxtail millet seedlings. Haplotype analysis of SiAOX4 and SiAOX5 revealed that these genes are in linkage disequilibrium, with Hap_2 being the superior haplotype for both, potentially conferring enhanced cold stress tolerance in the cultivar group. These findings suggest that both SiAOX4 and SiAOX5 may be targeted for selection in future breeding programs aimed at improving foxtail millet's resilience to cold stress.

Performance Characterization of the Field Aged Asphalt Pavement Materials at Mortar Scale: Testing Method Optimization and Rheology Analysis

Performance evaluation at the asphalt mortar scale offers increased sensitivity to aging and is less affected by extraction processes comparated to asphalt mixture. Despite these benefits, there is currently a lack of standardized methods for directly processing mortar samples obtained from field mixtures. This study aims to improve and validate a manufacturing procedure for aged mortar specimens derived from field aging cores, and to evaluate the field damage level of asphalt paving materials at the mortar scale. A novel approach was developed that incorporates warm sieving, compaction, coring, and cutting to manufacture mortar specimens suitable for Dynamic Shear Rheometer (DSR) tests. This study selected mortar specimens with different service ages and conducted rheological tests, including frequency sweep tests and modified Multiple Stress Creep Recovery (MSCR) tests, to assess their rheological performance. And the significance test of all the indcies were conducted to select suitable indcies.The results show the proposed aged mortar manufacture approach can prepare eligible mortar specimens for rheology tests. The rheology test results show that several indices can distinguish different mortar types and service ages, and the indices of surface layer mortar follow a linear relationship with service age. The linear viscoelastic zone (LVE), representative shear modulus (G0), and unrecovered creep compliance at 1.6kPa (Jnr1.6) and 3.2kPa (Jnr3.2) are identified as effective indices for evaluating the behavior of field aged mortar because of their effectiveness, lower variability, and better distinction.

Exploring the transcription start sites and other genomic features facilitates the accurate identification and annotation of small RNAs across multiple stress conditions in Mycobacterium tuberculosis.

Mycobacterium tuberculosis (MTB) is a pathogen that is known for its ability to persist in harsh environments and cause chronic infections. Understanding the regulatory networks of MTB is crucial for developing effective treatments. Small regulatory RNAs (sRNAs) play important roles in gene expression regulation in all kingdoms of life, and their classification based solely on genomic location can be imprecise due to the computational-based prediction of protein-coding genes in bacteria, which often neglects segments of mRNA such as 5'UTRs, 3'UTRs, and intercistronic regions of operons. To address this issue, our study simultaneously discovered genomic features such as TSSs, UTRs, and operons together with sRNAs in the M. tuberculosis H37Rv strain (ATCC 27294) across multiple stress conditions. Our analysis identified 1,376 sRNA candidates and 8,173 TSSs in MTB, providing valuable insights into its complex regulatory landscape. TSS mapping enabled us to classify these sRNAs into more specific categories, including promoter-associated sRNAs, 5'UTR-derived sRNAs, 3'UTR-derived sRNAs, true intergenic sRNAs, and antisense sRNAs. Three of these sRNA candidates were experimentally validated using 3'-RACE-PCR: predictedRNA_0240, predictedRNA_0325, and predictedRNA_0578. Future characterization and validation are necessary to fully elucidate the functions and roles of these sRNAs in MTB. Our study is the first to simultaneously unravel TSSs and sRNAs in MTB and demonstrate that the identification of other genomic features, such as TSSs, UTRs, and operons, allows for more accurate and specific classification of sRNAs.

Multiple Role Conflicts In The Employment Field In Indonesian Government Agency Institutions with Work Life Balance (WLB) and Job Stress As Mediator Variables on Employee Performance

Work-life balance issues are an important aspect of the psychology of happiness, which is determined by a strong sense of self-worth, satisfaction, and a variety of life-affirming relationships. This study aims to explore how dual role conflicts affect employee performance in the Manpower Office (Disnaker) in Indonesia, by considering the role of work-life balance (WLB) and work stress as mediator variables. The research method used involved collecting data from 154 employees in the disnaker through questionnaires and interviews. The data was then analyzed using statistical analysis techniques to identify the relationship between multiple role conflicts, WLB, work stress, and employee performance. The results showed that dual role conflict had a significant effect on WLB and work stress, but did not affect employee performance levels. Meanwhile, work stress and WLB cannot mediate the dual role with performance. This research provides important insights for human resource management in government agencies to develop policies and programs that support the balance between work and personal life of employees. An emphasis on managing multiple role conflicts and work stress in the work environment can improve employee well-being as well as the overall effectiveness of the organization. The practical implications of this study are the importance of appropriate interventions to improve the working conditions of employees in the public sector, while improving quality public services.

Genome-wide identification and expression analysis of the SPL gene family and its response to abiotic stress in barley (Hordeum vulgare L.)

BackgroundSquamosa promoter-binding protein-like (SPL) is a plant-specific transcription factor that is widely involved in the regulation of plant growth and development, including flower and grain development, stress responses, and secondary metabolite synthesis. However, this gene family has not been comprehensively evaluated in barley, the most adaptable cereal crop with a high nutritional value.ResultsIn this study, a total of 15 HvSPL genes were identified based on the Hordeum vulgare genome. These genes were named HvSPL1 to HvSPL15 based on the chromosomal distribution of the HvSPL genes and were divided into seven groups (I, II, III, V, VI, VII, and VIII) based on the phylogenetic tree analysis. Chromosomal localization revealed one pair of tandem duplicated genes and one pair of segmental duplicated genes. The HvSPL genes exhibited the highest collinearity with the monocotyledonous plant, Zea mays (27 pairs), followed by Oryza sativa (18 pairs), Sorghum bicolor (16 pairs), and Arabidopsis thaliana (3 pairs), and the fewest homologous genes with Solanum lycopersicum (1 pair). The distribution of the HvSPL genes in the evolutionary tree was relatively scattered, and HvSPL proteins tended to cluster with SPL proteins from Z. mays and O. sativa, indicating a close relationship between HvSPL and SPL proteins from monocotyledonous plants. Finally, the spatial and temporal expression patterns of the 14 HvSPL genes from different subfamilies were determined using quantitative real-time polymerase chain reaction (qRT-PCR). Based on the results, the HvSPL gene family exhibited tissue-specific expression and played a regulatory role in grain development and abiotic stress. HvSPL genes are highly expressed in various tissues during seed development. The expression levels of HvSPL genes under the six abiotic stress conditions indicated that many genes responded to stress, especially HvSPL8, which exhibited high expression under multiple stress conditions, thereby warranting further attention.ConclusionIn this study, 15 SPL gene family members were identified in the genome of Hordeum vulgare, and the phylogenetic relationships, gene structure, replication events, gene expression, and potential roles of these genes in millet development were studied. Our findings lay the foundation for exploring the HvSPL genes and performing molecular breeding of barley.

The Physiological Mechanism of Exogenous Melatonin on Improving Seed Germination and the Seedling Growth of Red Clover (Trifolium pretense L.) under Salt Stress.

Salt stress can affect various physiological processes in plants, ultimately hindering their growth and development. Melatonin (MT) can effectively resist multiple abiotic stresses, improving plant stress resistance. To analyze the mechanism of exogenous MT to enhance salt tolerance in red clover, we conducted a comprehensive study to examine the influence of exogenous MT on various parameters, including seed germination indices, seedling morphological traits, and physiological and photosynthetic indicators, using four distinct red clover varieties (H1, H2, H3, and H4). This investigation was performed under various salt stress conditions with differing pH values, specifically utilizing NaCl, Na2SO4, NaHCO3, and Na2CO3 as the salt stressors. The results showed that MT solution immersion significantly improved the germination indicators of red clover seeds under salt stress. The foliar spraying of 50 μM and 25 μM MT solution significantly increased SOD activity (21-127%), POD activity, soluble sugar content, proline content (22-117%), chlorophyll content (2-66%), and the net photosynthetic rate. It reduced the MDA content (14-55%) and intercellular CO2 concentration of red clover seedlings under salt stress. Gray correlation analysis and the Mantel test further verified that MT is a key factor in enhancing seed germination and seedling growth of red clover under salt stress; the most significant improvement was observed for NaHCO3 stress. MT is demonstrated to improve the salt tolerance of red clover through a variety of mechanisms, including an increase in antioxidant enzyme activity, osmoregulation ability, and cell membrane stability. Additionally, it improves photosynthetic efficiency and plant architecture, promoting energy production, growth, and optimal resource allocation. These mechanisms function synergistically, enabling red clover to sustain normal growth and development under salt stress.

Genome-Wide Identification of CAMTA Gene Family in Oat (Avena sativa) and Expression Analysis under Various Abiotic Stresses

Oat (Avena sativa) is one of the most important cereal crops and cool-season forage grasses in the world. The calmodulin-binding transcription activator (CAMTA) gene family is one of the largest families in plants, and it plays vital roles in multiple biological processes. However, the CAMTA genes in oats, especially those involved in abiotic stress, have not yet been elucidated. Herein, our findings reveal the presence of 20 distinct AsCAMTA genes, which were clustered into three subfamilies based on their gene structure and conserved motifs, indicating functional similarities within each subgroup. Chromosomal mapping indicated an uneven distribution across 10 chromosomes, suggesting a complex evolutionary history marked by potential gene duplication events. The results showed that most AsCAMTA genes contained stress-related cis-elements. The study further investigated the expression patterns of these genes under abiotic stress conditions utilizing RT-qPCR analysis. The results identified three AsCAMTA genes (AsCAMTA5, AsCAMTA7, and AsCAMTA19) that exhibited significant up-regulation under salt stress, with AsCAMTA7 also showing a marked increase in expression under drought stress. These findings suggest a pivotal role of AsCAMTA5, AsCAMTA7, and AsCAMTA19 genes in mediating the responses to various abiotic stresses by integrating multiple stress signals in oats. This investigation provides valuable insights into the potential functions of AsCAMTA genes in the stress response mechanisms of oats, laying a foundation for further functional studies aimed at enhancing abiotic stress tolerance in crops.

Genomic Organization and Expression Profiling of GOLDEN2-like Transcription Factor Genes in Eggplant and Their Role in Heat Stresses

GOLDEN2-like (GLK) transcription factor genes are involved in chloroplast biogenesis during all stages of plant growth and development, as well as in the response to biotic and abiotic stresses. However, little is known about this transcription factor family in eggplant. In this study, we identified 54 GLK genes in the eggplant genome (S. melongena L.) and classified them into seven groups (G1–G7). Structural analysis illustrated that the SmGLK proteins of specific groups are relatively conserved. Cis-acting elements indicated that these genes are likely to be involved in multiple responses stimulated by light, phytohormones, and abiotic stress. Collinear analysis indicated that expansion of the SmGLK gene family primarily occurred through segmental duplication. Tissue-specific expression analysis revealed that SmGLKs were preferentially expressed in leaves, fruits, and seeds. Further screening of SmGLK genes revealed their differential expression under various treatments. Notably, SmGLK18 was significantly responsive to multiple phytohormones and stress treatments, whereas SmGLK3 and SmGLK12 were highly induced by ABA, IAA, SA, and drought treatments. Our study provides new information on the eggplant GLK family systematically and comprehensively. For the first time, we propose that SmGLK18 may play a key role in improving heat resistance. This study provides valuable candidate gene resources for further functional research and will benefit eggplant molecular breeding.

Genome-Wide Analysis of the Histone Modification Gene (HM) Family and Expression Investigation during Anther Development in Rice (Oryza sativa L.).

Histone modification plays a crucial role in chromatin remodeling and regulating gene expression, and participates in various biological processes, including plant development and responses to stress. Several gene families related to histone modification have been reported in various plant species. However, the identification of members and their functions in the rice (Oryza sativa L.) histone modification gene family (OsHM) at the whole-genome level remains unclear. In this study, a total of 130 OsHMs were identified through a genome-wide analysis. The OsHM gene family can be classified into 11 subfamilies based on a phylogenetic analysis. An analysis of the genes structures and conserved motifs indicates that members of each subfamily share specific conserved protein structures, suggesting their potential conserved functions. Molecular evolutionary analysis reveals that a significant number of OsHMs proteins originated from gene duplication events, particularly segmental duplications. Additionally, transcriptome analysis demonstrates that OsHMs are widely expressed in various tissues of rice and are responsive to multiple abiotic stresses. Fourteen OsHMs exhibit high expression in rice anthers and peaked at different pollen developmental stages. RT-qPCR results further elucidate the expression patterns of these 14 OsHMs during different developmental stages of anthers, highlighting their high expression during the meiosis and tetrad stages, as well as in the late stage of pollen development. Remarkably, OsSDG713 and OsSDG727 were further identified to be nucleus-localized. This study provides a fundamental framework for further exploring the gene functions of HMs in plants, particularly for researching their functions and potential applications in rice anthers' development and male sterility.

Genome-Wide Characterization of Alfin-like Genes in Brassica napus and Functional Analyses of BnaAL02 and BnaAL28 in Response to Nitrogen and Phosphorus Deficiency.

Alfin-like proteins (ALs) form a plant-specific transcription factor (TF) gene family involved in the regulation of plant growth and development, and abiotic stress response. In this study, 30 ALs were identified in Brassica napus ecotype 'Zhongshuang 11' genome (BnaALs), and unevenly distributed on 15 chromosomes. Structural characteristic analysis showed that all of the BnaALs contained two highly conserved domains: the N terminal DUF3594 domain and the C-terminal PHD-finger domain. The BnaALs were classified into four groups (Group I-IV), supported by conserved intron-exon and protein motif structures in each group. The allopolyploid event between B. oleracea and B. rapa ancestors and the small-scale duplication events in B. napus both contributed to the large BnaALs expansion. The promoter regions of BnaALs contained multiple abiotic stress cis-elements. The BnaALs in I-IV groups were mainly expressed in cotyledon, petal, root, silique, and seed tissues, and the duplicated gene pairs shared highly similar expression patterns. RNA-seq and RT-qPCR analysis showed that BnaALs were obviously induced by low nitrogen (LN) and low phosphorus (LP) treatments in roots. Overexpressing BnaAL02 and BnaAL28 in Arabidopsis demonstrated their functions in response to LN and LP stresses. BnaAL28 enhanced primary roots' (PRs) length and lateral roots' (LRs) number under LP and LN conditions, where BnaAL02 can inhibit LR numbers under the two conditions. They can promote root hair (RH) elongation under LP conditions; however, they suppressed RH elongation under LN conditions. Our result provides new insight into the functional dissection of this family in response to nutrient stresses in plants.

Rheological and Aging Properties of Nano-Clay/SBS Composite-Modified Asphalt.

Nano-organic montmorillonite (OMMT) not only inhibits the harmful asphalt fume generation during the production and construction processes of asphalt mixtures but also effectively improves the performance of asphalt pavements. In order to prepare asphalt materials with smoke suppression effects and good road performance, this study selects nano-OMMT and SBS-modified asphalt for composite modification of asphalt mixtures and systematically investigates its road performance. Through the temperature sweep test, the frequency sweep test, the multiple stress creep recovery (MSCR) test, the bending beam rheometer (BBR) test, and the atomic force microscope (AFM) test, the high-temperature rheological properties, low-temperature rheological properties, high-temperature properties and aging resistance of the modified asphalt are studied. The research findings indicate that OMMT can effectively reduce the sensitivity of modified asphalt to load stress and improve its high-temperature rheological properties. SBS-modified asphalt shows increased creep stiffness and a decreased creep rate after OMMT modification, resulting in reduced flexibility and decreased low-temperature crack resistance. After short-term and long-term aging, the complex modulus aging index of OMMT/SBS composite-modified asphalt is lower than that of SBS-modified asphalt, and the phase angle aging index is higher than that of SBS-modified asphalt, demonstrating that OMMT enhances the aging resistance of SBS-modified asphalt. OMMT inhibits oxidation reactions in the asphalt matrix, reducing the formation of C=O and S=O bonds, thereby slowing down the aging process of modified asphalt and improving its aging resistance.

P192 PREVALENCE OF HYPERTENSION AND ITS ASSOCIATED FACTORS AMONG PUBLIC VEHICLE DRIVERS IN POKHARA, NEPAL

Introduction: Hypertension, defined as >=140/90 mm Hg or currently taking antihypertensive medicine, is a major public health concern globally, affecting individuals of all ages, backgrounds, and lifestyles. Occupational risk factors associated with hypertension are poorly studied in the context of Nepal, drivers could be at a higher risk of hypertension because of multiple shift work, stress, inactivity, and poor diet. Methodology This cross-sectional study recruited 358 public vehicle drivers from Pokhara Metropolitan City of Nepal. The WHO STEPS questionnaire was used to assess the prevalence of hypertension. Data were entered into REDCap software and analyzed by using SPSS. The chi-square test and binary logistic regression were applied to identify hypertension-related factors. Results: The mean age of the participants was 37.3 ±8.5 years, and all of them were male. The prevalence of hypertension was found to be 41.9%. Using the chi-square test, hypertension was found to be associated with age, lower education, being married, tobacco consumption, age of smoking initiation, alcohol consumption, binge alcohol intake, lower physical activity, obesity, duration of driving, and service. However, when using binary logistic regression, the association was found only with age (OR=0.353; 95% CI: 0.183 to 0.680) and obesity (OR=0.429; 95% CI: 0.234 to 0.783). Conclusion: This study concludes that nearly two-fifths of drivers had hypertension, which is higher than the prevalence in the general population. The research highlights the need to focus on risk factors associated with hypertension among public vehicle drivers in Pokhara, Nepal.

Counseling Psychologists Assist Post-Disaster Psychological Rescue Experience in Major Disasters

<p>近年國內多起重大災難事件造成個體與社會群體的影響與不安，諮商心理師身為專業醫事人員提供受災者災後心理救援，也衍生災難事件危機介入的實踐與困境。本研究以現象學的研究方法、半結構式訪談蒐集資料，受訪者為六位諮商心理師，其中三位具督導資格，研究結果包含二類與八個主題：（1）諮商心理師災後心理救援經驗，包含：（a）災後心理救援前的自我評估與專業知能；（b）災後心理救援時的角色與功能；（c）災後心理救援時的預備、初期、中期、中長期作為與督導者作為；（d）災後心理救援後的多重困境、身心壓力與文化民俗挑戰。（2）因應災後心理救援困境的實務規劃，包含（a）落實演練與心理教育；（b）持續增能與案例討論；（c）建立名單與督導關係；（d）熟悉雲端與資訊操作；（e）調整期待與服務心態。作者依研究結果提出災後心理救援「停、看、問」三個步驟與九項建議：（1）「停」—介入之前：（a）自評身心狀態與投身意圖；（b）具足所需專業知能與增能；（c）培育多元文化介入的能力。（2）「看」—介入當下：（a）理解角色功能適才適性；（b）調整不同階段介入作為；（c）理解困境挑戰正面因應。（3）「問」—介入之後：（a）身心自我調適與回歸原有平衡；（b）尋求督導協助重整介入經驗；（c）系所課程培育與繼續教育增能，最後提及未來研究建議。</p> <p> </p><p>Since the ""Taiwan Disaster Year"" began with the 921 earthquake, Taiwan has witnessed numerous significant disaster events in recent years, resulting in extensive financial losses and loss of life. These events have profoundly affected individuals’ trust and sense of security, leading to a widespread impact on social groups and cultures. As professional healthcare providers, counseling psychologists frequently engage in post-disaster psychological rescue and reconstruction efforts during major disasters or crises. This practice has raised challenges for crisis intervention during such events. Researchers thus aim to understand the landscape and challenges of psychological rescue efforts during domestic disasters. This study uses phenomenological research methods and semi-structured interviews to gather data. It includes interviews with six counseling psychologists, three of whom are certified supervisors who acted as supervisors during disaster events, while the other three provided crisis intervention services as psychologists. The study categorizes its findings into two main themes and eight sub-themes: Experiences of counseling psychologists in post-disaster psychological support: (1) Self-assessment and professional knowledge before providing post-disaster psychological support: This theme explores how psychologists evaluate their readiness and expertise before engaging in disaster relief efforts. (2) Roles and functions of post-disaster psychological support: This examines the specific responsibilities and activities undertaken by psychologists during the support process. (3) Preparatory, initial, mid-term, and long-term actions, including the role of supervisors: This delves into the different stages of psychological support and the crucial role of supervisors in guiding and supporting these efforts. (4) Multiple challenges, physical and mental stress, and cultural and folk challenges after providing post-disaster psychological support: This theme addresses the complex challenges psychologists face following their intervention, including stress and cultural issues. Practical planning to address the challenges of post-disaster psychological support: (1) Implementing drills and psychological education: Emphasizes the importance of preparedness through regular drills and education. (2) Continuous capacity building and case discussions: Highlights the need for ongoing training and discussion of cases to enhance skills and knowledge. (3) Establishing lists and supervisory relationships: Focuses on creating structured relationships and lists to streamline support efforts. (4) Familiarity with cloud and information operations: Stresses the need for proficiency in modern technology to facilitate efficient support. (5) Adjusting expectations and service mentality: Discusses the necessity of adapting expectations and maintaining a service-oriented mindset. Based on the research results, the researcher proposes a systematic approach comprising three steps (""Stop, Look, Ask"") and nine specific suggestions for post-disaster psychological support, serving as a practical reference for crisis intervention practice: ""Stop""—before intervention: (1) Self-assess physical and mental state and willingness to engage: Psychologists need to evaluate their readiness and motivation. (2) Equip with necessary professional knowledge and capacity building: Psychologists need to ensure that they have the required expertise and training. (3) Cultivate the ability for multicultural intervention: Develop skills to effectively engage with diverse cultural backgrounds. ""Look""—during intervention: (1) Understand the roles and functions and fit them appropriately: Matching roles and tasks to individuals’ strengths. (2) Adjust intervention actions at different stages: Modify approaches according to the phase of intervention. (3) Understand challenges and respond positively: Recognize and constructively address difficulties encountered. ""Ask""—after intervention: (1) Physical and mental selfadjustment and return to original balance: Focusing on recovery and well-being. (2) Seek supervisory assistance to reorganize intervention experiences: Utilizing supervision to reflect and improve.(3) Curriculum development and continued education for capacity building: Advocating for ongoing education and training in disaster psychology. Finally, the study concludes with recommendations for future research and suggests areas for further exploration to enhance the effectiveness of psychological support in disaster scenarios.</p> <p> </p>

Plant Growth Promoting Rhizobacteria (PGPR) induced protection: A plant immunity perspective.

Plant-environment interactions, particularly biotic stress, are increasingly essential for global food security due to crop losses in the dynamic environment. Therefore, understanding plant responses to biotic stress is vital to mitigate damage. Beneficial microorganisms and their association with plants can reduce the damage associated with plant pathogens. One such group is PGPR (Plant growth-promoting rhizobacteria), which influences plant immunity significantly by interacting with biotic stress factors and plant signalling compounds. This review explores the types, metabolism, and mechanisms of action of PGPR, including their enzyme pathways and the signalling compounds secreted by PGPR that modulate gene and protein expression during plant defence. Furthermore, the review will delve into the crosstalk between PGPR and other plant growth regulators and signalling compounds, elucidating the physiological, biochemical, and molecular insights into PGPR's impact on plants under multiple biotic stresses, including interactions with fungi, bacteria, and viruses. Overall, the review comprehensively adds to our knowledge about PGPR's role in plant immunity and its application for agricultural resilience and food security.

Simulation and field application study of roadway remodeling load-bearing arch technology

This paper presents a numerical simulation study on the impact of material strength, thickness, and lateral pressure coefficient on the load-bearing arches used in tunnel reconstruction, as well as industrial experiments based on these arches. The study compares three different cross-sectional shapes—straight-wall semicircular arches, flat-bottom straight-wall semicircular arches, and inverted straight-wall semicircular arches—through multi-dimensional numerical simulations investigating various parameters such as material strength, thickness, and lateral pressure coefficient. We analyze the maximum load-bearing capacity of the reconstructed arches and the evolution characteristics of the plastic zones in the tunnel support structures with different cross-sectional shapes. The results indicate that among the factors affecting the ultimate load-bearing capacity of the arches, the ranking is as follows: tensile strength > thickness > lateral pressure coefficient. Additionally, the shape of the cross-section significantly influences the evolution of plastic zones during tunnel failure. The technology of reconstructing load-bearing structures in coal mine tunnels effectively addresses the deformation issues caused by the superimposition of multiple stresses in the tunnels of Daniu Coal Mine. This provides valuable insights for understanding and managing significant deformations resulting from concentrated stresses from in-situ rock, tectonic forces, and mining-induced stresses.

Evaluation of Rheological Properties of Asphalt Binder Modified with Biochar from Oat Hulls.

In this study, the effect of biochar from oat hulls (BO) on the rheological properties of a PG 64-22 asphalt binder was evaluated using a full factorial design, which included the following factors: pyrolysis temperature (PT) (300 °C and 500 °C), BO particle size (<20 µm and <75 µm), and the amount of BO (2.5%, 5%, and 7.5%). First, the morphological and physicochemical properties of BO were analyzed by comparing it with graphite powder (CFG) and commercial activated carbon (CAC). The physicochemical properties of the modified asphalt binder were then evaluated using confocal laser microscopy, scanning electron microscopy (SEM-EDX), and Fourier-transform infrared spectroscopy (FTIR). Its storage stability was also evaluated. Subsequently, the rutting parameter G*/sin(δ) and the Fraass breaking point were analyzed to select asphalt binders that extended their viscoelastic range. The asphalt binders selected were those with 2.5%, 5%, and 7.5% BO, produced at a PT of 300 °C with a particle size <20 µm (BO300S). Next, the rheological properties of the selected samples were evaluated by testing for rotational viscosity, rutting parameter G*/sin(δ), multiple stress creep recovery (MSCR), fatigue parameter G*·sin(δ), and creep stiffness by bending beam rheometry (BBR). The rheological aging index according to rutting parameter G*/sin(δ) (RAI) was also evaluated. These tests were conducted in different states of the asphalt binder: original, short-term aged, and long-term aged. According to the results, the application of BO300S significantly increased the resistance of the asphalt binder to rutting and rotational viscosity, proportional to the amount added to the asphalt binder. Moreover, low modifier percentages improved fatigue resistance, outperforming CFG and CAC. In addition, it performs well at low service temperatures, registering better resistance than the control asphalt binders.

Study on the Storage Stability and Rheological Property of Bio-Oil/Lignin Composite-Modified Asphalt.

The objective of this study is to investigate the storage stability and rheological property of bio-oil/lignin composite-modified asphalt. The composite-modified asphalt with different proportions of bio-oil was prepared and cured at 105 °C, 135 °C, and 165 °C for 24 h and 48 h. The storage stability of the composite-modified asphalt was evaluated based on the softening point difference, the storage stability index derived from rotational viscosity, the segregation rate based on temperature sweep, and the non-recoverable creep compliance measured through the Multiple Stress Creep Recovery test. The storage stability of bio-oil/lignin composite-modified asphalt was evaluated through testing and analysis of its infrared spectroscopy and scanning electron microscopy before and after thermal storage. The research results indicate that the maximum difference in softening point is 0.9 °C, and the calculated storage stability index is generally below 0.1. The maximum value of the segregation rate is 0.43, indicating excellent storage stability of the bio-oil/lignin composite-modified asphalt. According to the results from infrared spectroscopy, no chemical reactions occurred during the storage process of the composite-modified asphalt. The scanning electron microscope confirmed that the samples became more stable after 48 h of storage.