Multiple Organic Solvents Research Articles

An automatic continuous-flow spotting/striping machine

This paper presents the principle, design and application of an automatic continuous-flow spotting/striping machine. The apparatus was specifically designed to apply multiple organic solvent extracts automatically and simultaneously as spots or stripes to chromatography paper. The machine applied twelve 300-μl volumes of a methanol—dichloromethane (4:1) solution to chromatography paper in 30 min as uniform and compact stripes. The dynamic action of a unique mechanical “heart-shaped” cam was responsible for obtaining uniform and compact stripes. A recovery range of 93–98% for [ 3H]aldosterone (in methanol—dichloromethane) was obtained after pumping the solution through Acidflex® and polyethylene tubing. The remaining [ 3H]aldosterone was removed by two 100-μl methanol—dichloromethane washes. Urine aldosterone values showed no significant differences when extracts were applied by the machine or manually. This machine reduces the man-hours required for sample applications three-fold and reduces operator tension built up by long hours of tedious manual applications.

A manifold for concentrating to dryness multiple organic solvent samples simultaneously and rapidly for thin-layer or gas chromatography

A manifold for concentrating to dryness multiple organic solvent samples simultaneously and rapidly for thin-layer or gas chromatography