Multiple Oral Squamous Cell Carcinoma Research Articles

Inhibition of Ca2+ entry by capsazepine analog CIDD-99 prevents oral squamous carcinoma cell proliferation.

Oral cancer patients have a poor prognosis, with approximately 66% of patients surviving 5-years after diagnosis. Treatments for oral cancer are limited and have many adverse side effects; thus, further studies are needed to develop drugs that are more efficacious. To achieve this objective, we developed CIDD-99, which produces cytotoxic effects in multiple oral squamous cell carcinoma (OSCC) cell lines. While we demonstrated that CIDD-99 induces ER stress and apoptosis in OSCC, the mechanism was unclear. Investigation of the Bcl-family of proteins showed that OSCC cells treated with CIDD-99 undergo downregulation of Bcl-XL and Bcl-2 anti-apoptotic proteins and upregulation of Bax (pro-apoptotic). Importantly, OSCC cells treated with CIDD-99 displayed decreased calcium signaling in a dose and time-dependent manner, suggesting that blockage of calcium signaling is the key mechanism that induces cell death in OSCC. Indeed, CIDD-99 anti-proliferative effects were reversed by the addition of exogenous calcium. Moreover, electrophysiological properties further established that calcium entry was via the non-selective TRPC1 channel and prolonged CIDD-99 incubation inhibited STIM1 expression. CIDD-99 inhibition of calcium signaling also led to ER stress and inhibited mitochondrial complexes II and V in vitro. Taken together, these findings suggest that inhibition of TRPC mediates induction of ER stress and mitochondrial dysfunction as a part of the cellular response to CIDD-99 in OSCC.

Simultaneous Presentation of Conventional and Pigmented Squamous Cell Carcinomas of the Oral Cavity

Simultaneous presentation of multiple oral squamous cell carcinomas (OSCCs) is a rare event, with less than 15 cases reported in the literature. We contribute an additional case of simultaneous conventional and pigmented OSCC. A 55-year-old man with a history of tobacco and alcohol consumption presented with 2 simultaneous lesions of unknown evolution—one was an asymptomatic 2-cm ulcer in the left border of the tongue and other was an erythroleukoplakia with pigmented areas measuring 1.5 cm in the right retromolar region. Both lesions were microscopically diagnosed as squamous cell carcinoma, the latter being classified as the pigmented variant of OSCC. The patient is stable, with no signs of recurrence 6 months after oncologic surgical treatment. Clinicians should consider the possibility of simultaneous occurrence of multiple OSCCs.

Clonal analysis as a prognostic factor in multiple oral squamous cell carcinoma

ObjectivesA novel classification based on molecular methods to assess clonality defines three types of secondary oral squamous cell carcinoma (OSCC): second primary tumour (SPT) independent from the index tumour, local recurrence (LR), clonally related to the primary tumour, and second field tumour (SFT), derived from the same genetically altered mucosal field as the primary tumour. The present study applied mtDNA analysis in a group of patients experiencing a second loco-regional neoplastic manifestation. The purpose was to differentiate secondary tumours into LRs, SPTs and SFTs and evaluate the prognostic impact in terms of survival rate. Material and methodsThe study population comprised 23 patients who experienced a second neoplastic lesion after a surgical resection of primary OSCC. mtDNA D-loop analysis was applied in paired neoplastic lesions and in clinically and histologically normal mucosa. On the basis of mtDNA results, the second OSCC was classified as LR or SPT or SFT. Disease-free survival was defined as the duration between the appearance of the second neoplastic lesion and death of disease, or last follow-up visit. ResultsSeven secondary tumours were classified as LR, 12 as SFT, 4 as SPT. An altered mucosal field proved a variable significantly related to a better survival rate (p<0.05); 2/12 (16.6%) SFT events failed as compared to 5/7 LRs (71.4%) and 3/4 SPTs (75%). ConclusionmtDNA analysis may be considered a useful tool to differentiate secondary tumours and might influence the choice of the most appropriate treatment in patients with multiple OSCCs.

Genomic characterisation of canine papillomavirus type 17, a possible rare cause of canine oral squamous cell carcinoma

Squamous cell carcinomas (SCCs) are the second most common cancer of the canine oral cavity resulting in significant morbidity and mortality. Recently a dog with multiple oral SCCs that contained a novel papillomavirus (PV) was reported. The aim of the present study was to determine the genome of this novel PV. To do this a short section of PV DNA was amplified from an oral SCC and ‘back-to-back’ primers were designed. Due to the circular nature of PV DNA, these primers were then used to amplify the remainder of the genome by inverse PCR. The PCR product was sequenced using next generation sequencing and the full genome of the PV, consisting of 8007bp, was assembled and analysed. As this is the seventeenth PV identified from the domestic dog, the novel PV was designated Canis familiaris papillomavirus (CPV) type 17. Similar to other CPV types, the putative coding regions of CPV-17 were predicted to produce 5 early and 2 late proteins. Phylogenetic analysis of ORF L1 revealed greater than 70% similarity to CPV-2 and CPV-7 and we propose that CPV-17 also be classified as a Taupapillomavirus 1. While it appears CPV-17 is only rarely present in canine oral SCCs, evidence suggests that this PV could influence the development of oral SCCs in this species.

Synchronous multiple oral squamous cell carcinomas: Report of a case

Long-term survival of head and neck cancer patients has not significantly improved in the last 30-year, in spite of advances in therapy. An important reason for this lack of progress is the development of second primary tumors (SPTs) in the upper aerodigestive tract. To account for the development of multiple (multicentric) primary tumors in the oral cavity, the concept of field cancerization by Slaughter can be interpreted in various ways to explain this phenomenon of SPTs. The frequency of developing synchronous and metachronous carcinomas in the head and neck mucosal sites ranges from 8% to 21%. These SPTs are usually more aggressive, more treatment resistant, and metastasizes early, requiring a more aggressive treatment strategy and hence the early detection is important. Here, we report a case of synchronous oral squamous cell carcinomas in a female patient, which was poorly differentiated histologically and was confirmed by immunohistochemical analysis.

骨髄移植後の慢性GVHD患者に発生した多発性口腔扁平上皮癌の1例

骨髄移植後の慢性GVHD患者に発生した多発性口腔扁平上皮癌の1例

Multiple oral squamous cell carcinomas following bone marrow transplantation

Multiple oral squamous cell carcinomas following bone marrow transplantation

Multiple oral squamous cell carcinomas associated with autoimmune polyendocrinopathy–candidiasis–ectodermal dystrophy

Autoimmune polyendocrinopathy-candidiasis-ectodermal dystrophy (APECED) is a rare autosomal recessive disorder characterized by chronic mucocutaneous candidiasis, hypoparathyroidism, and adrenal insufficiency. Chronic oral candidiasis is frequently the first manifestation of the condition. There is an increased incidence of oral squamous cell carcinomas at an early age in this population, and it is possible that chronic oral candidal infection has a role in oral carcinogenesis in patients with APECED. We present a case of multiple oral squamous cell carcinomas in a 35-year-old woman with chronic mucocutaneous candidiasis as a component of APECED. Our patient has had 3 confirmed oral squamous cell carcinomas to date, which have been managed with laser resection and ablation. She remains on systemic antifungal therapy and is under regular surveillance in a multidisciplinary head and neck clinic.

Clinical study of multiple primary and double cancers including oral squamous cell carcinoma

ObjectiveIn recent years, there has been an increase in the incidence of multiple and double cancers involving the oral cavity. In this study, we clinically reviewed patients with multiple or double cancers. Patients and methodsThe subjects were 696 patients with multiple oral squamous cell carcinomas who had visited the Department of Oral and Maxillofacial Surgery, Tokyo Dental College during the 26-year period between 1982 and 2008 and 497 patients with double cancers treated during the 16-year period from 1992, when endoscopy was started, to 2008. ResultsThe incidence of multiple primary cancers was 26 (3.7%) in 696 patients. The most common primary site of multiple cancers was the mandibular gingiva (42.3%). The maximum number of multiple cancers per patient was seven. On the other hand, the incidence of double cancers was 43 (8.7%) in 497 patients, with esophageal cancer being the most frequent (58.1%). The most common primary site of oral cancers in double cancer patients was the oral floor (32.6%). ConclusionsAbout 10% of all patients developed double cancers. Therefore, follow-up is necessary not only for recurrence and metastasis, but also for the development of a primary cancer elsewhere through strengthening endoscopy in close cooperation with the endoscopist.

Multiple Oral Squamous Cell Carcinomas With Blood and Tissue Eosinophilia

Blood eosinophilia (BE) is occasionally associated with malignant tumors and has been accepted as a marker for disseminated carcinoma with poor prognosis.1,2 Local eosinophilia of the surrounding stroma of a carcinoma also has been reported in various sites, including the head and neck.1,3,4 There has been controversy as to whether tumor-associated tissue eosinophilia (TATE) is indicative of prognosis.5-8 Recently, TATE has been proposed as an indicator of a favorable prognosis.4,9

Specific Identification of Telomerase Activity and p53 Gene Product in Metachronous Multiple Oral Squamous Cell Carcinoma and Adjacent Tissues

Objectives: To define the association of telomerase activity, histopathological characteristics, and p53 gene product expression in metachronous oral squamous cell carcinomas.Patients and Methods: Three regions of tissue specimens from the tumour, the region adjacent to the tumour, and intact oral mucosa from the surgical margin from 2 patients were simultaneously evaluated. Telomerase activity was determined by telomeric repeat amplification protocol assay. Hematoxylin and eosin stain and DO7 monoclonal antibody were used to stain formalin-fixed, paraffin-embedded sections to examine the histopathological features and p53 gene product expression.Results: Marked telomerase activity was expressed in tumour tissue and in adjacent dyskeratotic tissue. No telomerase activity was found in hyperkeratotic or dysplastic oral mucosa. Although p53 gene product was detected in the tumour and adjacent regions of both patients, it was found in the intact oral mucosa of only 1 patient.Conclusion: Estimation of tissue telomerase activity and p53 gene product expression is helpful in identifying malignancy in oral mucosal lesions.

Oral lichenoid lesions caused by allergy to mercury in amalgam fillings

Oral lichenoid lesions (OLL) or lichen-planus-like lesions are often idiopathic. Our aim was to determine whether OLL can be caused by allergy to mercury in amalgam fillings, and whether resolution of OLL occurs after replacement of amalgam with other dental fillings. Patients with only OLL (except for 1 case with cutaneous lichen planus) referred for patch testing during 1985-1994 to the Contact and Occupational Dermatitis Clinic of the Skin & Cancer Foundation, Darlinghurst, were reviewed. Patch tests were performed with 1% mercury, 1% ammoniated mercury, 0.1% thimerosal, 0.1% mercuric chloride, 0.05% phenylmercuric nitrate and an amalgam disc, using Finn Chambers occluded for 2 days, 19 patients (17 women and 2 men; age range: 28-72 years) had OLL in close contact with amalgam fillings and showed positive patch test reactions to mercury compounds, 16 out of 19 patients had their amalgam fillings replaced. In 13 patients, the OLL healed. 1 patient had marked improvement. 1 patient had no improvement and developed multiple oral squamous cell carcinoma. In conclusion, OLL can be caused by allergy to mercury in amalgam fillings. Replacement of amalgam with other dental fillings usually results in resolution of OLL and is recommended for cases with positive patch test reactions to mercury compounds.

Sweet's syndrome and malignancy: a case associated with multiple oral squamous cell carcinoma

Sweet's syndrome and malignancy: a case associated with multiple oral squamous cell carcinoma