You have accessJournal of UrologyKidney Cancer: Advanced (including Drug Therapy) II1 Apr 2018MP66-11 RETROSPECTIVE ANALYSIS OF THE CONCORDANCE OF 20 IMMUNOHISTOCHEMICAL TISSUE MARKERS IN METASTATECTOMY LESIONS IN PATIENTS WITH METASTATIC RENAL CELL CARCINOMA Sung Han Kim, Eun Young Park, Ho Kyung Seo, Jae Young Joung, Kang Hyun Lee, Yoon Seok Suh, Jungnam Joo, and Jinsoo Chung Sung Han KimSung Han Kim More articles by this author , Eun Young ParkEun Young Park More articles by this author , Ho Kyung SeoHo Kyung Seo More articles by this author , Jae Young JoungJae Young Joung More articles by this author , Kang Hyun LeeKang Hyun Lee More articles by this author , Yoon Seok SuhYoon Seok Suh More articles by this author , Jungnam JooJungnam Joo More articles by this author , and Jinsoo ChungJinsoo Chung More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2018.02.1883AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES Renal cell carcinoma (RCC) is a heterogenous and multicentric cancer with poor survival once it progresses to metastasis. The standard therapy for metastatic RCC (mRCC) is systemic targeted therapy which has differential therapeutic responses according to the metastatic lesions and metastatic organs. This study is aimed to characterize different expressions of 20 tissue markers in multiple metastatic lesions and organs in mRCC patients. METHODS A total of 66 mRCC patients with their 179 metastatectomy tissue lesions (MTL) were enrolled with their medical records. The immunohistochemistry was performed for tissue microarrays using 20 tissue markers including BAP1, CD31, CD 34, HIF-1a, HIF-2a, Ki-67, pS6, PBRM1, PDGFRa and b, PD-L1, PSMA, PTEN, a-SMA, TGase2, VEGFR-1,-2,-3, VHL loss, and CA9. The cases were identified pathologically by a senior uropathologist, the semi-quantitative H-score (0-300), including intensity score (0,1,2,3). The intensity score was divided into two categories (negative or positive) to examine the concordance rate of the expressions of markers between different organs, and different sites of the same organ. The concordance rate was calculated as the number of patients with concordant intensity score out of the total number of patients in that comparison. RESULTS A total of 66 patients were divided into patients with the same organs and different organs. 42 patients (44 cases) with 96 MTL, and 39 patients with 83 MTL were used to examine the concordance of the markers within the same and different organs, respectively. Among 179 MTL, 84 Kidney, 43 Lung, 25 Bone, 7 Brain, 5 LN and 15 other organs were stained with tissue markers. Within the same organs, the BAP1, PSMA, VEGFR3, PDGFRa (>0.9 concordance rate), PS6, HIF2, and (>0.8 concordance rate), PDL1, HIF1 (> 0.7 concordance rate) were highly concordant expressions even though there are from different MTL. As for the expression of different metastatic organs of a same patient, BAP1, PSMA, HIF1, VEGFR3 (>0.9 concordance rate), PDGFRa, SMA (>0.8 concordance rate), PS6, PDL1, and PTEN(>0.7 concordance rate) showed consistent result in spite of the fact that they were obtained from different organs. For 26 patients who recurred within the same organ, markers were stained again, and MTL were analyzed to examine the concordance of the recurrent site within the same organ. BAP1, PSMA (>0.9 concordance rate), PS6, VEGFR3, PDGFRa (>0.8), PTEN and HIF2 (>0.7 concordance rate) showed concordance. However, there was a limitation that the distribution of some high concordant markers is shifted to one side. CONCLUSIONS The high concordant tissue markers including BAP1, VEGFR3, PDGFRa, PSMA (PS6 : Concordance rate >0.7) showed similar expression of markers regardless of metastatic organs, metastatic sites or recurrence, whereas other markers were influenced by other factors. © 2018FiguresReferencesRelatedDetails Volume 199Issue 4SApril 2018Page: e871-e872 Advertisement Copyright & Permissions© 2018MetricsAuthor Information Sung Han Kim More articles by this author Eun Young Park More articles by this author Ho Kyung Seo More articles by this author Jae Young Joung More articles by this author Kang Hyun Lee More articles by this author Yoon Seok Suh More articles by this author Jungnam Joo More articles by this author Jinsoo Chung More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...