Multinutrient Supplement Research Articles

Effects of a multinutrient-fortified milk drink combined with exercise on functional performance, muscle strength, body composition, inflammation, and oxidative stress in middle-aged women: a 4-month, double-blind, placebo-controlled, randomized trial

Multinutrient protein-enriched supplements are promoted to augment the effects of exercise on muscle mass and strength, but their effectiveness in middle-aged women, or whether there are any additional benefits to physical function, remains uncertain. We aimed to evaluate whether a multinutrient-fortified milk drink (MFMD) could enhance the effects of exercise on functional muscle power (stair climbing) in middle-aged women. Secondary aims were to evaluate the intervention effects on physical function, muscle strength, lean mass (LM), fat mass (FM), bone mineral content (BMC), muscle cross-sectional area (CSA), muscle density, balance, flexibility, aerobic fitness, inflammation, oxidative stress, bone and cartilage turnover, blood pressure, and blood lipids. In this 4-mo, double-blind, placebo-controlled, randomized trial, 244 women (45-65 y) participated in a multimodal resistance-type exercise program 3 d/wk, with random allocation to a twice-daily MFMD containing added protein, vitamin D, calcium, milk fat globule membrane (phospholipids and other bioactives), and other micronutrients (Ex+MFMD, n=123) or an energy-matched placebo (Ex+placebo, n=121). A total of 216 women (89%) completed the study. After 4 mo, both groups experienced similar 3.6%-4.3% improvements in the primary outcomes of fast-pace 5- and 10-step stair ascent power. In contrast, Ex+MFMD experienced greater improvements in 5-step regular-pace stair descent time [net difference (95% CI): -0.09 s (-0.18, 0.00 s), P=0.045], countermovement jump height [0.5 cm (0.04, 1.0 cm), P=0.038], total body LM [0.3 kg (0.04, 0.60 kg), P=0.020], FM [-0.6 kg (-1.0, -0.2 kg), P=0.004], BMC [0.4% (0.1%, 0.6%), P=0.020], muscle CSA [thigh: 1.8% (0.6%, 2.9%), P=0.003; lower leg: 0.9% (0.3%, 1.6%), P=0.005], balance eyes closed [3.3 s (1.1, 5.4 s), P=0.005], 2-min step performance [8 steps (3, 12 steps), P=0.003], and sit-and-reach flexibility [1.4 cm (0.6, 2.2 cm), P=0.026]. MFMD did not enhance the effects of exercise on any measures of muscle strength, gait speed, dynamic balance, reaction time, or blood lipids, and there was no effect of either intervention on blood pressure, markers of inflammation, or cartilage turnover. Ex+placebo had a greater improvement in the oxidative stress marker protein carbonyls (P<0.01). In middle-aged women, daily consumption of an MFMD did not enhance the effects of a multimodal exercise program on the primary outcome of stair climbing ascent power, but did elicit greater improvements in multiple secondary outcomes including various other measures of functional performance, LM, muscle size, FM, balance, aerobic capacity, flexibility, and bone metabolism.This trial was registered at www.anzctr.org.au as ACTRN12617000383369.

Using Metabolomics to Classify the Underlying Effects of Multi-Nutrient Supplementation in ADHD Youth

Abstract Objectives Attention-deficit/hyperactivity disorder (ADHD) is a neuropsychiatric disorder commonly diagnosed in childhood. Current pharmaceutical treatment options provide a poor long-term risk: benefit ratio with little knowledge of the long-term effects. A broad-spectrum multi-nutrient formula has shown promise in children, but its effects on nutrient status and the underlying metabolome interactions have not been characterized. Methods Blood samples from medication-free children (n = 74) with ADHD enrolled in a double–blind randomized placebo-controlled multinutrient trial (RCT) were collected at baseline and 8 weeks post-intervention. Following RCT is an 8-week open label phase during which all participants took the active supplement. Symptoms were assessed using the Child and Adolescent Symptom Inventory 5. Double-blinded plasma samples will be analyzed for tyrosine, phenylalanine, tryptophan, magnesium, and zinc. Untargeted LC-MS metabolomics using HILIC chromatography and a high resolution QTof will assess very polar analytes in plasma extracts. Linear modeling will elucidate the influence of treatment, sampling time, and ADHD symptom score on plasma nutrient and plasma metabolite concentration. Results Preliminary findings of the open label phase show a significant improvement in inattention (P = 0.0435), hyperactivity (P = 0.0068), ODD (P = 0.0108) and DMDD (P = 0.0119). We hypothesize that these improvements in ADHD symptoms will be correlated with increased circulating concentrations of tyrosine, phenylalanine, tryptophan, magnesium, zinc, and metabolites involved in neurotransmitter synthesis and/or branched chain amino acid metabolism. Conclusions Preliminary findings indicate improvements of ADHD symptoms of inattention, hyperactivity, ODD and DMDD following 8 weeks of open label multi-nutrient supplementation. Results of the double-blinded phase are expected to mirror those observed in the open label phase, with increases in nutrients in those receiving the multinutrient. Funding Sources The sample analyses were supported by NIH Award Number Grant P30 CA016058, OSU, and OSUCCC. Foundation for the Center of Excellence in Mental Health, Canada; The Ohio State University Department of Human Sciences, College of Education and Human Ecology; The Ohio State University Wexner Medical Center, Clinical Research Center.

The Effects of the Dietary Supplement Cardioflex on Reducing Cardiovascular Disease Risk Factors in Adults

Abstract Objectives The overall objective was to determine the effects of the multi-nutrient dietary supplement Cardioflex, independent of diet or lifestyle changes, to reduce cardiovascular disease (CVD) risk factors in adults. Methods A 90-day, randomized, double-blind, parallel design clinical study was performed from February to June 2019. Participants received one serving (10 g) of Cardioflex or an isocaloric maltodextrin placebo daily. Blood pressure was measured using an automatic sphygmomanometer. A 1-min arterial test (accelerated plethysmography; APG) and a 5-min heart-rate-variability (HRV) test were conducted using a Meridian digital pulse wave analyzer. Serum concentrations of total cholesterol (TC), triglycerides (TG) and high-density lipoprotein (HDL) were measured using Vitros-350 chemistry analyzer. Analysis of covariance was used to compare the post treatment biomarkers of the two groups using IBM SPSS Statistics (v.25). Results A total of 67 participants (placebo = 33, Cardioflex = 34) with a mean age of 48.3 ± 8.8 years, completed the study. Cardioflex improved HRV (+5%; P = 0.04) compared to the placebo (−9%). Although both groups saw improvements in blood pressure, heart rate, APG age, body mass index, waist circumference, TC, low-density lipoprotein (LDL), and TG, there was no significant difference (P &amp;gt; 0.05) between groups. Interestingly, the placebo significantly (P = 0.04) lowered (−18.7%) TC compared to Cardioflex (−15%). Conclusions Cardioflex supplement may benefit HRV; a parameter associated with CVD. However, more research is needed to understand its effects on CVD. Also, our study also highlights the ‘placebo effect’ that can occur in parallel design clinical trials. Funding Sources Funding was provided by MITACs and Innotech Nutrition.

Multinutrient Supplementation Increases Collagen Synthesis during Early Wound Repair in a Randomized Controlled Trial in Patients with Inguinal Hernia

Inguinal hernia disease is associated with an imbalanced collagen metabolism. Surgical stress has a negative impact on nutrients important for collagen synthesis. We hypothesized that supplementation with a combination of nutrients would enhance collagen biosynthesis in inguinal hernia disease patients when undergoing hernia repair. In this exploratory randomized controlled trial, 21 men (age: 55.2±2.8 y; BMI: 25.0±0.7kg/m2) scheduled for Lichtenstein inguinal hernia repair were assigned to multinutrient supplementation (n=10; multinutrient group) or no multinutrient supplementation (n=11; control group). The multinutrient group received 14g l-arginine, 14g l-glutamine, 1250mg vitamin C, and 55mg zinc daily starting 14 d before surgery and ending 14 d after surgery. The multinutrient and control groups received high-quality protein to ensure a daily intake of 1.5g protein/kg. Collagen biosynthesis was measured by the biomarkers type I procollagen propeptide (CICP), type III procollagen propeptide (PRO-C3), and type V procollagen propeptide (PRO-C5) in the sera on days -14, 0, and 1, and in the wound fluids on postoperative days 1 and 2. Compliance was recorded after the 28-d intervention period. Serum PRO-C5 concentrations decreased (P <0.05) postoperatively in the control but not the multinutrient group. Neither CICP nor PRO-C3 serum concentrations differed significantly between the 2 groups. In wound fluid, the CICP concentrations increased (P <0.05) from days 1 to 2 in the multinutrient group and were 49% higher (P=0.10) than those in the control group on day 2. Wound fluid concentrations PRO-C3 and PRO-C5 showed no significant time or group differences. The 28-d compliance was similar (P=0.27) in the 2 groups. Oral supplementation with arginine, glutamine, vitamin C, and zinc augment collagen synthesis during the first 2 d after inguinal hernia repair. This trial was registered at clinicaltrials.gov as NCT03221686.

Effect of molasses based multi-nutrient herbal supplements on in vitro digestibility, serum enzymes and minerals profile in buffalo calves

Molasses based multinutrients herbal supplements (MMS) containing ground fenugreek seed and de-oiled mahua seed cake at two different ratios (1:1; MMS I or 1:3; MMS II) was evaluated under in vitro as well as in vivo conditions. For in vitro evaluation, concentrate mixture and wheat straw in 50: 50 ratio (on DM basis) was taken as substrate for the experiment. MMS I and MMS II were added at 2, 3 and 4% of the substrate, respectively. The total gas production (ml/g DM), partitioning factor (PF) and microbial biomass production (MBP) were found comparable in all the groups but significantly improved the IVDMD and IVOMD in supplemented groups. Under in vivo feeding trial, serum enzymes and minerals profile in buffalo calves were assessed during 9 months of experimental period. Fifteen male Murrah buffalo calves (10 to 15 months of age and mean body wt. 234.0±12.5 kg) were randomly distributed into 3 groups (5 in each group) according to randomized block design. All animals were fed with conventional concentrate mixture, available chopped green fodder (3-4 kg DM/d) and wheat straw ad lib to meet out nutrients requirement. While control group (C) were not fed supplements but groups T1 and T2 supplemented with MMS I and MMS II, respectively at 44 g/100 kg body weight or 200 g/100 kg metabolic body weight (kgW0.75). Serum AST, ALT activities and serum concentration of Ca, P, Fe, Mn, Cu and Co were comparable among 3 groups but serum concentration of Zn was significantly higher among supplemented groups. Thus, results show that supplementation of MMS improved IVDMD, IVOMD and serum Zn level resulting in to better health conditions of buffalo calves.

Effects of a high-DHA multi-nutrient supplement and exercise on mobility and cognition in older women (MOBILE): a randomised semi-blinded placebo-controlled study.

There is a complex interplay between mobility and cognition in older adults. We have previously shown that a high-DHA multi-nutrient supplement improves habitual walking speed, verbal memory and psychomotor response latency in older women. Exercise also improves mobility and cognition in older adults, and n-3 fatty acids and exercise share a range of overlapping biological effects. This study examined for the first time the effects of the high-DHA multi-nutrient supplement and aerobic exercise on mobility and cognition in older women. Women (mean age 67 (sd 8) years) were assigned to the following groups: multi-nutrient (1 g DHA, 160 mg EPA, 240 mg Ginkgo biloba, 60 mg phosphatidylserine, 20 mg d-α tocopherol, 1 mg folic acid and 20 µg vitamin B12 per d, n 13), multi-nutrient and exercise (spin class twice per week, n 14), exercise and placebo (n 12) or placebo (n 12). The multi-nutrient was given for 24 weeks and exercise for 12 weeks. No treatment effects were observed for the primary outcome, habitual walking speed. Improvements in verbal memory and executive function were seen for all treatments groups v. placebo (all, P < 0·05). Significant improvements in self-reported emotional well-being were seen with multi-nutrient and exercise groups v. placebo (P = 0·03). The results suggest that the high-DHA multi-nutrient supplement produces similar improvements in cognitive function to aerobic exercise, offering the intriguing prospect that supplementation may be able to mitigate some of the effects of low physical activity on cognitive function in the elderly.

Effect of molasses based multi-nutrient herbal supplements on hematobiochemicals, serum lipid, antioxidants and hormonal profile in buffalo calves

Effect of molasses based multinutrients herbal supplements (MMS) containing ground fenugreek seed and deoiled mahua seed cake at two different ratios (1:1; MMS-I or 1:3; MMS-II) on hematobiochemicals, serum lipid, antioxidants and hormonal profile in male buffalo calves was assessed for 9 months. Fifteen male Murrah buffalo calves (10 to 15 months of age and mean body wt. 234–236 kg) were randomly distributed into 3 groups (5 each) according to Randomized Block Design (RBD). All animals were fed individually with conventional concentrate mixture, available green fodder (3–4 kg DM/d) and wheat straw ad lib. to meet out nutrients requirement. While animals in control group (C) were fed no supplement but animal’s diet in group T1 and T2 supplemented with MMS-I and MMS-II, respectively at 44 g/100 kg body weight or 200 g /100 kg metabolic body weight (kgW0.75). The values of Hb, PCV, RBC, WBC, platelets count, serum total protein, uric acid, creatinine, urea level, total cholesterol, triglyceride, high density lipoprotein (HDL) and T3 hormone level were comparable among 3 groups. Serum glucose, globulin, antioxidants and testosterone levels were significantly increased but serum albumin, A/G ratio, low density lipoprotein (LDL) and cortisol levels were significantly decreased among supplemented groups as compared to control group and hormone T4 was significantly higher in T1 group. The results showed that supplementation of MMS-I and MMS- II in the diet of buffalo calves improved serum glucose, globulin, antioxidants, T4 and testosterone levels but lower albumin, A/G ratio, LDL and cortisol.

Effect of Organic Basic Multrinutrient Block Supplementation on Total Mixed Ratio of Kacang Goat in Feedlot System

The research was managed to assess organic basic multi-nutrient block supplementation on the performance of Kacang goat that fed by total mixed ratio in feedlot system. This research was carried out for 3 months, used 15 goats with the average body weight of 13.40 ± 1.97 kg. The study was arranged in a completely randomized design with 3 treatments and 5 replications. Goats were divided and fed with one of the treatments as follows: P0: only forage, according to the farmer’s way; P1: total mixed ratio; P2: total mixed ratio + 15g multinutrient block/head/day. Variables of initial body weight, final body weight, body weight gain, and feed consumption were observed. The study indicated that goats of P1 and P2 had a significantly higher final body weight in average of 29,32 and 32,38 kg (P < 0.05) compared with P0 (27,45 kg), respectively. Body weight gain of goat P2 was significantly higher (P < 0.05) than P1 Kacang goat. This study suggests that treatment P2 resulted in the highest body weight gain.

Effect of molasses based multi-nutrient herbal supplements on the performance of buffalo calves

An experiment was conducted to study the effect of molasses based multi-nutrient herbal supplements (MMS) containing ground fenugreek seed and de-oiled mahua seed cake at two different ratios (1:1; MMS-I and 1:3; MMS-II) on growth performance, nutrient utilization and immune response of male buffalo calves. Fifteen male Murrah buffalo calves (10 to 15 months of age and mean body wt. 234–236 kg) were randomly distributed into 3 groups of 5 each. All animals were supplied with conventional concentrate mixture, green forages (3–4 kg DM/d) and wheat straw ad lib. to meet out nutrient requirement. Animals in control group were not fed supplement but animals in group T1 and T2 were fed MMS-I and MMS-II, respectively @ 200 g/100 kg metabolic body weight (kg W0.75). The intake and digestibility of DM, OM, EE, NDF, ADF, hemicelluloses, cellulose and purine derivatives in urine were comparable among 3 groups. Digestibility of crude protein (CP) was higher in supplemented groups (T1 and T2) than control. Total dry matter intake, body weight gain, average daily gain (ADG) and feed conversion ratio (FCR) during 270 days were comparable among 3 groups. Both cellular and humoral immune response improved in T1 and T2 groups. The results revealed that molasses based multi-nutrient herbal supplement containing fenugreek and DMSC improved the growth performance, nutrient utilization and immune responses.

MON-PO597: The Effect of a Perioperative Multinutrient Supplement on Collagen Synthesis During Early Hernia Repair: A Randomised Clinical Pilot Study

MON-PO597: The Effect of a Perioperative Multinutrient Supplement on Collagen Synthesis During Early Hernia Repair: A Randomised Clinical Pilot Study

Multinutrient supplements found to have no benefit in preventing depression onset

Multinutrient supplementation did not show preventive effects for depression compared with food‐related behavioral activation therapy or no therapy in a group of overweight adults with subsyndromal symptoms of depression, a randomized trial involving more than 1,000 participants has found. Hospitalization rates during the trial's one‐year follow‐up were virtually identical in the three study groups, the researchers reported. Study results were published March 5 in JAMA.

Multinutrients for ADHD Youth (MADDY) Trial: Preliminary Trends for Treatment (P16-028-19)

ObjectivesAttention-deficit/hyperactivity disorder (ADHD) is a neuropsychiatric disorder with increasing global prevalence and high heritability commonly diagnosed in childhood. Current pharmaceutical treatment options provide a poor long-term risk: benefit ratio. Nutrition has significant effects on neurological functioning, and increasing evidence exists to support the use of multinutrients as alternative treatment for ADHD. The goal of this study is to assess the efficacy and tolerability of a multinutrient supplement as an alternative treatment for ADHD and co-occurring mood dysregulation symptoms. MethodsThe Multinutrients in ADHD Youth study is a fully-funded, multi-site, randomized double-blinded clinical trial for adolescents ages 6–12 years (n = 135). The study is 16 weeks in total, with an 8-week randomized controlled trial (RCT) followed by an 8-week open label (OL) phase. During the RCT phase, participants are assigned to either the treatment or placebo group at a 3:2 ratio. Study outcomes of ADHD symptoms include child’s inattention, hyperactivity and mood dysregulation, which are assessed using validated questionnaires. Outcome data for the OL phase of the study were analyzed using end of RCT phase as baseline given that the RCT component of the study is still blinded. ResultsPreliminary findings based on the OL phase compared ADHD symptoms following 8 weeks of open label supplementation (n = 27 families). A significant decrease in symptom count was found for inattention (P ≤ 0.001), hyperactivity (P ≤ 0.001), ODD (P ≤ 0.001) and DMDD (P ≤ 0.001). ConclusionsThis study is the first adequately powered RCT in North America to investigate the effects of multinutrients supplements on ADHD symptoms among children. Preliminary findings indicate a trend in the improvements of ADHD symptoms of inattention, hyperactivity, ODD and DMDD in the OL phase of the study. Funding SourcesFoundation for the Center of Excellence in Mental Health, Canada; The Ohio State University Department of Human Sciences, College of Education and Human Ecology; The Ohio State University Wexner Medical Center, Clinical Research Center.

Effects of non-medicated and medicated urea molasses multi-nutrient block supplements on nutrient intake and blood mineral profile of lactating Saanen goats.

Poor nutrition is a major contributor to the performance of dairy goats on smallholder farms. Hence, strategic supplementation is required to overcome nutritional deficiencies. This paper reviews the effects of urea molasses multi nutrient block (UMMB) and medicated urea molasses multi-nutrient block (MUMB) supplements in comparison with imported commercial mineral blocks (CMB) on nutrient intake and blood mineral profile of Saanen lactating goats. A 90-day feeding trial was conducted at Yusof Ecofarm, Tanah Merah, Kelantan, Malaysia. Twenty four (24) Saanen lactating goats were assigned to four dietary treatments with six (6) goats in each group based on completely randomized block design. Goats in T1 (control) were fed with basal diet only that comprise of roughages and goat pellet while T2, T3 and T4 received equal amount of basal diet and allowed for ad-libitum licking of UMMB, MUMB and CMB respectively. Total dry matter intake (DMI) (kg/d) were 1.14, 1.29, 1.25 and 1.16 in T1, T2, T3 and T4 respectively whereas the UMMB, MUMB and CMB intakes (g/d) were 86.8, 50.4 and 36.6 respectively. There were significant differences (p&lt;0.05) between treatments on nutrient intake. Crude protein (CP), crude fiber (CF), ether extract (EE), acid detergent fiber (ADF) and neutral detergent fiber (NDF) were significantly higher (p&lt;0.05) in T2 and T3 as compared to T1 and T4. The serum concentrations of calcium (Ca), ferum (Fe), copper (Cu) and zinc (Zn) were significantly higher (p&lt;0.05) in T2 with the values of 33.60 ppm, 341.67 ppb, 270.00 ppb and 138.30 ppb respectively as compared to other treatments. Hence, the findings of this research revealed that both UMMB and MUMB were effective in improving nutrient intake and blood mineral profile of lactating Saanen goats.

Combining a high DHA multi-nutrient supplement with aerobic exercise: Protocol for a randomised controlled study assessing mobility and cognitive function in older women

There is a complex interplay between cognition and gait in older people, with declines in gait speed coexisting with, or preceding cognitive decline. Omega-3 fatty acids, B vitamins, vitamin E, phosphatidylserine, and Ginkgo Biloba show promise in preserving mobility and cognitive function in older adults. Exercise benefits mobility and there is evidence suggesting positive interactions between exercise and omega-3 fatty acids on physical and cognitive function in older adults. Non-frail or pre-frail females aged ≥60 years are included in a randomized placebo controlled study. Intervention groups are: high DHA multi-nutrient supplement and exercise, placebo supplement and exercise, high DHA multi-nutrient supplement, and placebo supplement. Dietary supplementation is 24 weeks. The exercise intervention, two cycle ergometer classes per week, is for the final 12 weeks. The primary outcome is habitual walking speed, secondary outcomes include gait variables under single and dual task, five times sit to stand, verbal and spatial memory, executive function, interference control and health related quality of life. Blood fatty acids, serum homocysteine, dietary intake, physical activity, and verbal intelligence are measured to assess compliance and control for confounding factors. The study is registered at www.clinicaltrials.gov (NCT03228550).

Effect of Multinutrient Supplementation and Food-Related Behavioral Activation Therapy on Prevention of Major Depressive Disorder Among Overweight or Obese Adults With Subsyndromal Depressive Symptoms

Effects of nutritional interventions on the prevention of major depressive disorder (MDD) in overweight adults are unknown. To examine the effect of 2 nutritional strategies (multinutrient supplementation, food-related behavioral activation therapy) and their combination for prevention of a new MDD episode in overweight adults with subsyndromal depressive symptoms. This multicenter 2 × 2 factorial randomized clinical trial included overweight adults (body mass index, 25-40) with elevated depressive symptoms (Patient Health Questionnaire-9 [PHQ-9] scores ≥5) and no MDD episode in the past 6 months from 4 European countries. A total of 1025 adults were randomized (July 30, 2015-October 12, 2016) and followed up for 1 year (October 13, 2017). Daily multinutrient supplements (1412-mg omega-3 fatty acids, 30-μg selenium, 400-μg folic acid, and 20-μg vitamin D3 plus 100-mg calcium) vs placebo and 21 individual or group therapy sessions vs none (blinded to researchers) for 1 year. Participants were allocated to placebo without therapy (n = 257), placebo with therapy (n = 256), supplements without therapy (n = 256), and supplements with therapy (n = 256). Cumulative 1-year onset of MDD via the Mini International Neuropsychiatric Interview at 3, 6, and 12 months. Logistic regression using effect-coded variables (-1 indicating control, 1 indicating intervention) evaluated intervention effects both individually and in combination (interaction) on MDD onset. Among 1025 participants (mean age, 46.5 years; 772 women [75%]; mean BMI, 31.4), 779 (76%) completed the trial. During the 12-month follow-up, 105 (10%) developed MDD: 25 (9.7%) patients in the placebo without therapy, 26 (10.2%) in the placebo with therapy, 32 (12.5%) in the supplement without therapy, and 22 (8.6%) in the supplement with therapy group. None of the treatment strategies affected MDD onset. The odds ratio (OR) for supplements was 1.06 (95% CI, 0.87-1.29); for therapy, 0.93 (95% CI, 0.76-1.13); and for their combination, 0.93 (95% CI, 0.76-1.14; P for interaction, .48). One person in the supplementation with therapy group, died. Twenty-four patients in each of the placebo groups and 24 patients in the supplementation with therapy group were hospitalized, and 26 patients in the supplementation-only group were hospitalized. Among overweight or obese adults with subsyndromal depressive symptoms, multinutrient supplementation compared with placebo and food-related behavioral activation therapy compared with no therapy did not reduce episodes of major depressive disorder during 1 year. These findings do not support the use of these interventions for prevention of major depressive disorder. ClinicalTrials.gov Identifier: NCT02529423.

The Effect of a Polysaccharide-Based Multinutrient Dietary Supplementation Regimen on Infections and Immune Functioning in Multiple Sclerosis

Multiple sclerosis (MS) is a progressive neurodegenerative disease associated with increased infection rates, chronic inflammation, and premature death. Optimization of nutritional status via dietary supplementation may improve immune function in people suffering from MS and lead to decreased rates of infection. Fifteen individuals with a diagnosis of relapsing-remitting MS for an average of 12.4 years (SD =7.4; R = 2, 25) were enrolled in a one-year open-label clinical trial. Participants consumed a broad-spectrum dietary supplement regimen containing polysaccharides, phytochemicals, antioxidants, vitamins, and minerals three times per day. The occurrence of infections and a panel of cytokines, growth factors, and T- and B-cell subsets were assessed at baseline and 12 months. Seven female and 8 male participants with an average age of 51.3 years (SD =7.2; R = 38, 65) completed the study. At the end of the intervention, participants had fewer total infections (M = 7.9, SD =8.1 at baseline and M = 2.5, SD =4.3 at 12-month follow-up). At 12 months, IL-2, TNF-α, EGF, and CD95 + CD34+ significantly increased, while IL-1β significantly decreased. No major adverse effects were reported; only mild gastrointestinal intolerance was reported in four cases. A decreased occurrence of infection was observed in MS patients treated with 12 months of a polysaccharide-based multinutrient dietary supplement. Significant changes were also noted in several key biomarkers that would be physiologically favorable to the MS population. Thus, the results of this study suggest an immunomodulatory effect of the dietary supplement regimen studied.

Effects of Medicated and Non-medicated Multi-nutrient Block Supplementation on Gastrointestinal Parasite Infestation and Blood Hematological Parameters of Lactating Saanen Goats

In a 90-day feeding trial, twenty four (24) Saanen lactating does were assigned to four treatment group. The treatments were feeding: basal diet only as a control (T1), basal diet with urea molasses multi-nutrient block (UMMB) (T2), basal diet with medicated urea molasses multi-nutrient block (MUMB) (T3) and basal diet with commercial mineral block (CMB) (T4). There were significant differences (p 0.05) between treatments on WBC, LYM, MON, GRA, RBC, HCT, MCV, MCH, PLT, MPV and PCT. However, significant effects (p<0.05) on HBG, MCHC, RDW and PDW were observed in T2 and T3. This research showed that UMMB and MUMB were effective in controlling parasite infestation in Saanen lactating dairy goats apart from improving their blood hematological parameters. Comparison with CMB showed that it is practical to be used for parasite control.

For older adults recovering from hip fracture surgery, what are the effects of multinutrient supplements?

For older adults recovering from hip fracture surgery, what are the effects of multinutrient supplements?

Effect of multi-nutrient liquid supplement on serum minerals and hormone profile in buffalo heifers

Eighteen Murrah buffalo heifers (average BW of 290±2.5 kg) were randomly distributed into three groups of six each in an experiment based on randomized block design to investigate the effect of partial replacement of concentrate mixture with molasses based multi-nutrient liquid supplements (MMLS) on serum mineral and reproductive hormonal profile. During 240 d of experimental period, all animals were supplied with green forages (2 kg DM/d) and wheat straw ad libitum. Animals in group T1 (control) were fed a concentrate mixture (maize 40, wheat bran 40, soyabean meal 17, mineral mixture 2, and salt 1%) to meet their nutrient requirements. However, 20% of the concentrates were replaced with MMLS and MMLS plus in groups T2 and T3, respectively. Blood was collected on d 0, 120 and 240 days of experiment to evaluate serum mineral and reproductive hormonal (progesterone and estradiol) profile. Serum concentrations of Ca, P, Fe and Mn were similar among the groups. Serum concentration of Cu and Zn was higher in groups T2 and T3. Concentrations of progesterone and estradiol were similar among the groups, but showed an increasing trend on day 240. It was concluded that partial replacement of concentrate mixture with MMLS and MMLS plus improved Cu and Zn status without any adverse impact on other minerals and reproductive hormone profile.

Association between cognitive function and supplementation with omega-3 PUFAs and other nutrients in ≥ 75 years old patients: A randomized multicenter study.

A few studies have assessed the association between omega-3 polyunsaturated fatty acids (n-3 PUFA) and cognitive impairment (CI) in very old adults. The aim of this study was to evaluate the effect of a multinutrient supplementation rich in n-3 PUFA on the cognitive function in an institutionalized ≥75-year-old population without CI or with mild cognitive impairment (MCI). A multicenter placebo-controlled double-blind randomized trial was conducted between 2012 and 2013. Cognitive function was assessed at baseline and after one year using 4 neuropsychological tests. Nutritional status was assessed using Mini Nutritional Assessment (MNA). Interaction between Mini-Mental State Examination (MMSE) score and nutritional status were analyzed using linear regression models. A total of 99 participants were randomized to receive placebo or pills rich in n-3 PUFA. After 1-year follow-up, both groups decreased their MMSE score (-1.18, SD:0. 53 and -0.82, SD:0. 63, p = 0.67 for the control and the intervention group respectively). The memory subscale of the MMSE showed an improvement (+0.26, SD:0.18) in the intervention group against a worsening in the control group (-0.11, SD: 0.14; p = 0.09 for differences between groups). Patients at intervention group with normal nutritional status (MNA ≥24) showed an improvement in the MMSE (+1.03, p = 0.025 for differences between 1-y and baseline measurements) against a worsening in the group with malnutrition (MNA<24) (-0.4, p = 0.886 for differences between 1-y and baseline; p of interaction p = 0.05). Supplementation with n-3 PUFA did not show an improvement in the global cognitive function in institutionalized elderly people without CI or with MCI. They only suggest an apparent improvement in memory loss if previously they were well nourished.