Multifunctional Biomolecule Research Articles

Natural tea extract coated porous MOF nano/microparticles for highly enhanced and selective adsorption of cationic dyes from aqueous medium

A simple, sustainable method was developed for boosting the dye adsorption capability of porous metal organic frameworks (MOFs) by coating them with natural tea extract. Nano/microparticles of zinc-terephthalic acid (ZnTPA) MOFs exhibited only 40% rhodamine B (Rh B) dye adsorption. The dye adsorption capability was strongly enhanced (up to 95%) after coating with natural tea extract and highly selective cationic dye adsorption. The coating of multifunctional biomolecules onto the ZnTPA matrix was expected to facilitate strong intermolecular interaction between MOF surface and organic dyes, which contributed to strongly enhanced dye adsorption. ZnTPA/Tea MOFs not only exhibited enhanced dye adsorption but also showed high retention capability. The broader scope of tea extract coating methodology for cationic dye adsorption was also demonstrated using other MOFs matrix. The exhausted ZnTPA MOF was easily regenerated by sonication followed by re-dipping into tea extract. Tea extract coated ZnTPA MOFs was also used as column packing materials for removing cationic dyes by filtration. Thus, the present work reported a facile strategy for efficient cationic organic dyes removal from the aqueous medium.

Photosynthetic machinery and antioxidant enzymes system regulation confers cadmium stress tolerance to tomato seedlings pretreated with melatonin

Cadmium (Cd), a significant heavy metal, is widely distributed in the Earth's crust. However, little is known about its effects on plants and management measured. Melatonin (MEL) is a well-known multifunctional biomolecule; it is a powerful antioxidant molecule that increases a plant's abiotic stress tolerance. MEL enhances nickel, vanadium, and chromium tolerance in plants. This research aimed to examine the impact of Cd-stress on tomato plants and explore the possible contribution of MEL in improving the resistance of tomato plants towards Cd-stress. The current findings exhibited that MEL application significantly enhanced leaf photosynthesis, root structure, osmolytes accumulation, and seedling growth compared with only Cd treatment. MEL application decreased Cd transport from root to shoot, lowering Cd concentrations in leaves. In addition, MEL treatment increased catalase (CAT), superoxide dismutase (SOD), ascorbate peroxidase (APX), and glutathione reductase (GR) activity and decreased the level of hydrogen peroxide (H2O2), superoxide ion (O2·−) and malondialdehyde (MDA) in tomato plants. Considering findings, MEL may be used to increase plant growth and Cd stress tolerance while lowering the availability of Cd to plants.

The pathway of melatonin biosynthesis in common wheat (Triticum aestivum).

Melatonin (Mel) is a multifunctional biomolecule found in both animals and plants. In plants, the biosynthesis of Mel from tryptophan (Trp) has been delineated to comprise of four consecutive reactions. However, while the genes encoding these enzymes in rice are well characterized no systematic evaluation of the overall pathway has, as yet, been published for wheat. In the current study, the relative contents of six Mel-pathway-intermediates including Trp, tryptamine (Trm), serotonin (Ser), 5-methoxy tryptamine (5M-Trm), N-acetyl serotonin (NAS) and Mel, were determined in 24 independent tissues spanning the lifetime of wheat. These studies indicated that Trp was the most abundant amongthe six metabolites, followed by Trm and Ser. Next, the candidate genes expressing key enzymes involved in the Mel pathway were explored by means of metabolite-based genome-wide association study (mGWAS), wherein two TDC genes, a T5H gene and one SNAT gene were identified as being important for the accumulation of Mel pathway metabolites. Moreover, a 463-bp insertion within the T5H gene was discovered that may be responsible for variation in Ser content. Finally, a ASMT gene was found via sequence alignment against its rice homolog. Validations of these candidate genes were performed by in vitro enzymatic reactions using proteins purified following recombinant expression in Escherichia coli, transient gene expression in tobacco, and transgenic approaches in wheat. Our results thus provide the first comprehensive investigation into the Mel pathway metabolites, and a swift candidate gene identification via forward-genetics strategies, in common wheat.

Bioderived materials for stimuli-responsive, adaptive, and neuromorphic systems: A perspective

In this concept article, we review recent works on the use of biologically-derived materials, including biomolecules, hybrid biomolecular composites, and natural tissues for achieving stimuli-responsiveness, adaptability, and memory storage for potential neuromorphic computing applications. Unlike solid-state materials, bioderived platforms are often intrinsically modular, including possessing the ability to leverage the diversity of naturally multifunctional biomolecules, cornerstones in biological transduction, signaling, and learning. The primary neuromorphic functionality of bioderived systems considered here is memory resistance, as obtained through combinations of resistive switching, activity-dependent plasticity, and memory storage. In some cases, these capabilities are achieved in bio-derived systems by closely emulating the structure, function, or signaling mechanisms found in living neurons. Our review considers bioderived systems that span multiple length scales and levels of hierarchical complexity: from single-molecule enabled devices to intact tissues, interrogated either in vitro or in vivo on living organisms. Through this exercise, we offer our perspective on research opportunities in the development and use of synapse- and neuron-inspired bioderived systems, including the prospect of biocompatible, and even tissue-like, neuromorphic materials to smartly monitor and treat injury and disease, deliver therapeutics, and interpret neural activity. These capabilities could unlock a new generation of smart, adaptable bioderived composites that autonomously report, learn (i.e., classify, forecast), compute, and actuate in the direct vicinity of living cells and tissues, i.e. at the edge of biology.

Studies of functional properties of espin 1: Its interaction to actin filaments.

Actin is a multifunctional biomolecule that forms not only basic structural bodies such as filopodia and lamellipodia, but also large microvilli-like organelles like stereocilia. Actin consists of four sub-domains (S1, S2, S3, and S4), and the "target-binding groove" formed between S1 and S3 is the major binding site for various actin binding proteins. Actin filament dynamics are regulated by numerous actin binding proteins with different mechanisms of actin binding, assembly, and disassembly such as actin severing, branching, and bundling. Ectoplasmic specialization protein 1 (espin 1) is an actin binding and bundling protein that is specifically implicated in the elongation and stabilization of stereocilia as a binding partner with myosin III. However, little is known about the molecular structure, actin bundling, and stabilizing mechanism of espin 1; hence, we investigated the interaction between actin and espin 1 through structural data. In this study, we first purified human espin 1 in an E. coli system following a new detergent-free approach and then demonstrated the 2D structure of full-length espin 1 using transmission electron microscopy along with Nickel nitrilotriacetic acid nanogold labeling and 2D averaging using SPIDER. Furthermore, we also determined the espin 1 binding domain of actin using a co-sedimentation assay along with gelsolin and myosin S1. These findings are not only beneficial for understanding the actin binding and bundling mechanism of espin 1, but also shed light on its elongation, stabilization, and tip-localization mechanisms with myosin III. This study thus provides a basis for understanding the molecular structure of espin 1 and can contribute to various hearing-related diseases, such as hearing loss and vestibular dysfunction.

Oxytocin: A Multi-Functional Biomolecule with Potential Actions in Dysfunctional Conditions; From Animal Studies and Beyond.

Oxytocin is a hormone secreted from definite neuroendocrine neurons located in specific nuclei in the hypothalamus (mainly from paraventricular and supraoptic nuclei), and its main known function is the contraction of uterine and/or mammary gland cells responsible for parturition and breastfeeding. Among the actions of the peripherally secreted oxytocin is the prevention of different degenerative disorders. These actions have been proven in cell culture and in animal models or have been tested in humans based on hypotheses from previous studies. This review presents the knowledge gained from the previous studies, displays the results from oxytocin intervention and/or treatment and proposes that the well described actions of oxytocin might be connected to other numerous, diverse actions of the biomolecule.

From Snake Venoms to Therapeutics: A Focus on Natriuretic Peptides

Snake venom is a cocktail of multifunctional biomolecules that has evolved with the purpose of capturing prey and for defense. These biomolecules are classified into different classes based on their functions. They include three-finger toxins, natriuretic peptides, phospholipases and metalloproteinases. The focus for this review is on the natriuretic peptide (NP), which is an active component that can be isolated from the venoms of vipers and mambas. In these venoms, NPs contribute to the lowering of blood pressure, causing a rapid loss of consciousness in the prey such that its mobility is reduced, paralyzing the prey, and often death follows. Over the past 30 years since the discovery of the first NP in the venom of the green mamba, venom NPs have shown potential in the development of drug therapy for heart failure. Venom NPs have long half-lives, different pharmacological profiles, and may also possess different functions in comparison to the mammalian NPs. Understanding their mechanisms of action provides the strategies needed to develop new NPs for treatment of heart failure. This review summarizes the venom NPs that have been identified over the years and how they can be useful in drug development.

Synthetic and Biological Surfactants Used to Mitigate Biofouling on Industrial Facilities Surfaces

The adhesion of organisms to surfaces is an event that occurs in natural and artificial systems and is commonly known as bio-encrustation. The elimination of biofouling through less aggressive and ecological methods has aroused the interest of researchers and industries worldwide. Among the products with the greatest potential for altering surfaces, surfactants act at cell-cell and cell-surface interfaces, reducing surface hydrophobicity, the adherence of microorganisms, and the formation of biofilms. Biological surfactants have stood out for their versatility and proven effectiveness in recent years as valuable tools, especially due to their biodegradable characteristics when compared to synthetic surfactants. Thus, they can transform and modernize biotechnology by providing more robust antifouling technologies. This review discusses the possible roles and applications of synthetic and biological surfactants from different sources, such as biofouling reducing agents, addressing the potential alteration of surfaces, reducing surface tension, biological activities (antimicrobial, antibiofilm, and anti-adherence), biodegradability, and anticorrosion activity. Market trends and the potential uses of these multifunctional biomolecules in biofouling control are also cited, along with examples from the literature using biosurfactants as antifouling agents.

Multifunctional biomolecule nanostructures for cancer therapy.

Biomolecule-based nanostructures are inherently multifunctional and harbour diverse biological activities, which can be explored for cancer nanomedicine. The supramolecular properties of biomolecules can be precisely programmed for the design of smart drug delivery vehicles, enabling efficient transport in vivo, targeted drug delivery and combinatorial therapy within a single design. In this Review, we discuss biomolecule-based nanostructures, including polysaccharides, nucleic acids, peptides and proteins, and highlight their enormous design space for multifunctional nanomedicines. We identify key challenges in cancer nanomedicine that can be addressed by biomolecule-based nanostructures and survey the distinct biological activities, programmability and in vivo behaviour of biomolecule-based nanostructures. Finally, we discuss challenges in the rational design, characterization and fabrication of biomolecule-based nanostructures, and identify obstacles that need to be overcome to enable clinical translation.

Growth Factors VEGF-A165 and FGF-2 as Multifunctional Biomolecules Governing Cell Adhesion and Proliferation.

Vascular endothelial growth factor-A165 (VEGF-A165) and fibroblast growth factor-2 (FGF-2) are currently used for the functionalization of biomaterials designed for tissue engineering. We have developed a new simple method for heterologous expression and purification of VEGF-A165 and FGF-2 in the yeast expression system of Pichia pastoris. The biological activity of the growth factors was assessed in cultures of human and porcine adipose tissue-derived stem cells (ADSCs) and human umbilical vein endothelial cells (HUVECs). When added into the culture medium, VEGF-A165 stimulated proliferation only in HUVECs, while FGF-2 stimulated the proliferation of both cell types. A similar effect was achieved when the growth factors were pre-adsorbed to polystyrene wells. The effect of our recombinant growth factors was slightly lower than that of commercially available factors, which was attributed to the presence of some impurities. The stimulatory effect of the VEGF-A165 on cell adhesion was rather weak, especially in ADSCs. FGF-2 was a potent stimulator of the adhesion of ADSCs but had no to negative effect on the adhesion of HUVECs. In sum, FGF-2 and VEGF-A165 have diverse effects on the behavior of different cell types, which maybe utilized in tissue engineering.

Melatonin alleviates drought impact on growth and essential oil yield of lemon verbena by enhancing antioxidant responses, mineral balance, and abscisic acid content.

Melatonin has recently emerged as a multifunctional biomolecule with promising aspects in plant stress tolerance. The present study examined the effects of foliar-sprayed melatonin (0, 100, and 200 μM) on growth and essential oil yield attributes of lemon verbena (Lippia citriodora) under water-shortage (mild, moderate and severe). Results revealed that melatonin minimized drought effects on lemon verbena, resulting in improved growth and essential oils yield. Drought impositions gradually and significantly reduced several growth parameters, such as plant height and biomass, whereas melatonin application revived the growth performance of lemon verbena. Melatonin protected the photosynthetic pigments and helped maintain the mineral balance at all levels of drought. Melatonin stimulated the accumulation of proline, soluble sugars and abscisic acid, which were positively correlated with a better preservation of leaf water status in drought-stressed plants. Melatonin also prevented oxidative damages by enhancing the superoxide dismutase, ascorbate peroxidase and catalase activities. Furthermore, increased levels of total phenolic compounds, chicoric acid, caffeic acid and chlorogenic acid, as well as ascorbate and total antioxidant capacity in melatonin-sprayed drought-stressed plants indicated that melatonin helped verbena plants to sustain antioxidant and medicinal properties during drought. Finally, melatonin treatments upheld the concentrations and yield of essential oils in the leaves of lemon verbena regardless of drought severities. These results provided new insights into melatonin-mediated drought tolerance in lemon verbena, and this strategy could be implemented for the successful cultivation of lemon verbena, and perhaps other medicinal plants, in drought-prone areas worldwide.

Melatonin metabolism, signaling and possible roles in plants.

Melatonin is a multifunctional biomolecule found in both animals and plants. In this review, the biosynthesis, levels, signaling, and possible roles of melatonin and its metabolites in plants is summarized. Tryptamine 5-hydroxylase (T5H), which catalyzes the conversion of tryptamine into serotonin, has been proposed as a target to create a melatonin knockout mutant presenting a lesion-mimic phenotype in rice. With a reduced anabolic capacity for melatonin biosynthesis and an increased catabolic capacity for melatonin metabolism, all plants generally maintain low melatonin levels. Some plants, including Arabidopsis and Nicotiana tabacum (tobacco), do not possess tryptophan decarboxylase (TDC), the first committed step enzyme required for melatonin biosynthesis. Major melatonin metabolites include cyclic 3-hydroxymelatonin (3-OHM) and 2-hydroxymelatonin (2-OHM). Other melatonin metabolites such as N1 -acetyl-N2 -formyl-5-methoxykynuramine (AFMK), N-acetyl-5-methoxykynuramine (AMK) and 5-methoxytryptamine (5-MT) are also produced when melatonin is applied to Oryza sativa (rice). The signaling pathways of melatonin and its metabolites act via the mitogen-activated protein kinase (MAPK) cascade, possibly with Cand2 acting as a melatonin receptor, although the integrity of Cand2 remains controversial. Melatonin mediates many important functions in growth stimulation and stress tolerance through its potent antioxidant activity and function in activating the MAPK cascade. The concentration distribution of melatonin metabolites appears to be species specific because corresponding enzymes such as M2H, M3H, catalases, indoleamine 2,3-dioxygenase (IDO) and N-acetylserotonin deacetylase (ASDAC) are differentially expressed among plant species and even among different tissues within species. Differential levels of melatonin and its metabolites can lead to differential physiological effects among plants when melatonin is either applied exogenously or overproduced through ectopic overexpression.

Evaluation of Diverse Barley Cultivars and Landraces for Contents of Four Multifunctional Biomolecules with Nutraceutical Potential

Barley is long-identified as a functional food due to its content of micronutrients, β-glucans and vitamins. However, there is scant literature on a number of other nutritionally important biomolecules in the barley grain. This study determined the contents of four biomolecules, each with multiple known human and/or other animal health benefits, in the grains of 27 commercial barley cultivars and 7 landraces of barley from diverse countries of origin. These included the antioxidants, comprised of various vitamin E isomers and polyphenols, the osmoprotectant glycine betaine (GB) that protects cellular cytoplasm from osmotic shock, and the ‘plant stress hormone’ abscisic acid (ABA) which is endogenously expressed in humans and has multiple roles in physiology. All grains exhibited the presence of all biomolecules, suggesting they could potentially make some contribution to the health benefits of barley. The total vitamin E content varied between 19.20 - 54.56 μg/g DW, with α-tocotrienol being the major component (33.9 - 60.7%). The phenolics made up 3.21 - 9.73 mg gallic acid equivalent (GAE)/g DW, exceeding the amounts in the two major cereals, rice and wheat. GB ranged between 0.41-1.40 mg/g DW. The total vitamin E contents and GB typically exceeded those in corn. ABA ranged as 8.50 - 235.46 ng/g dry weight (DW), with the highest inter-variety variability. The data confirm barley to be an excellent source of these nutraceuticals, generally better than other major cereals. Our results thus offer more detailed insights into the potential of barley as a functional food and suggests the need to investigate in depth the health effects of this grain as well as the contribution of genetic and environmental factors.

A dual-functional peptide, Kpt from Ruegeria pomeroyi DSS-3 for protein purification and silica precipitation

Marine biological resources are significant sources of biological and chemical processes and principles from which new bioactive compounds can be explored. Here, we report a new silica-forming peptide (SFP) called Kpt (KPTHHHHHHDG) derived from a membrane protein in Ruegeria pomeroyi DSS-3, a heterotrophic marine bacterium found in coastal areas. The imidazole-, hydroxy-groups, and high positive charge of the Kpt sequence contribute to silica precipitation. Kpt exhibited high silica-precipitating activity at a pH 8.0 compared to other SFPs. Additionally, Kpt sustained its activity when was fused to N-terminus of ferritin (Fn), forming Kpt-Fn with unchanged caged structure. The results of this study suggest that Kpt can be used as a new SFP and a purification tag in fusion proteins by affinity chromatography. With its short sequence, Kpt is expected to have no effect on the main protein structure, which was fused by Kpt. Therefore, it is an ideal peptide for producing multi-functional biomolecules.

Integrated approaches to reveal mechanisms by which RNA viruses reprogram the cellular environment.

RNA viruses are major threats to global society and mass outbreaks can cause long-lasting damage to international economies. RNA and related retro viruses represent a large and diverse family that contribute to the onset of human diseases such as AIDS; certain cancers like T cell lymphoma; severe acute respiratory illnesses as seen with COVID-19; and others. The hallmark of this viral family is the storage of genetic material in the form of RNA, and upon infecting host cells, their RNA genomes reprogram the cellular environment to favor productive viral replication. RNA is a multifunctional biomolecule that not only stores and transmits heritable information, but it also has the capacity to catalyze complex biochemical reactions. It is therefore no surprise that RNA viruses use this functional diversity to their advantage to sustain chronic or lifelong infections. Efforts to subvert RNA viruses therefore requires a deep understanding of the mechanisms by which these pathogens usurp cellular machinery. Here, we briefly summarize several experimental techniques that individually inform on key physicochemical features of viral RNA genomes and their interactions with proteins. Each of these techniques provide important vantage points to understand the complexities of virus-host interactions, but we attempt to make the case that by integrating these and similar methods, more vivid descriptions of how viruses reprogram the cellular environment emerges. These vivid descriptions should expedite the identification of novel therapeutic targets.

Renoprotective Effects of Di- and Tri-peptides Containing Proline, Glycine and Leucine in Diabetes Model of Adult Mice: Enzymology and Histopathology

The small peptides, as multifunctional biomolecules, can affect some organs and metabolic disorders. The purpose of this experiment was to investigate the effects of some small peptides on the renal enzymes and histopathology in mice exposed to diabetes. Solid phase peptide synthesis method was used to synthesis di- and tri-peptides. Diabetes was induced in mice by multiple injection of low doses of streptozotocin. The effects of the di- and tri-peptides on renal aspartate aminotransferase, alanine transaminase, alkaline phosphatase, superoxide dismutase (SOD), glutathione peroxidase, total antioxidant (TAC), and catalase activities were evaluated by enzymatic biochemical methods and malondialdehyde (MDA) by thiobarbituric acid method. Hematoxylin and eosin and TUNEL stainings performed for assessing histopathology and apoptosis of the mice kidney. Hannaneh (Leu–Gly) and carnosine (Ala–His) exhibited suppression effects on the blood glucose elevation and the severity of the renal alterations in diabetic mice. They showed a decreasing effect on diabetes-induced DNA damage (P < 0.05). Carnosine significantly prevented from MDA elevation (P < 0.05). The Pro–Pro showed a supression effect on the blood glucose elevation (P < 0.05). Glutathione (Glu–Cys–Gly) and hannaneh caused to renal tissue TAC decreasing (P < 0.05). Mice receiving Pro–Gly and Pro–Gly–Pro have lesser renal SOD compared to diabetic mice (P < 0.05). Pro–Gly–Pro significantly decreased the diabetes-induced DNA damage. Although all the treatments had significant effects on alleviating the apoptosis, but carnosine and hannaneh dipeptides had the best protective effect on the kidney. The carnosine and hannaneh dipeptides had higher reno-protective effects against renal enzymes and histologic changes in diabetic mice.

Microbial Surfactants: The Next Generation Multifunctional Biomolecules for Applications in the Petroleum Industry and Its Associated Environmental Remediation.

Surfactants are a broad category of tensio-active biomolecules with multifunctional properties applications in diverse industrial sectors and processes. Surfactants are produced synthetically and biologically. The biologically derived surfactants (biosurfactants) are produced from microorganisms, with Pseudomonas aeruginosa, Bacillus subtilis Candida albicans, and Acinetobacter calcoaceticus as dominant species. Rhamnolipids, sophorolipids, mannosylerithritol lipids, surfactin, and emulsan are well known in terms of their biotechnological applications. Biosurfactants can compete with synthetic surfactants in terms of performance, with established advantages over synthetic ones, including eco-friendliness, biodegradability, low toxicity, and stability over a wide variability of environmental factors. However, at present, synthetic surfactants are a preferred option in different industrial applications because of their availability in commercial quantities, unlike biosurfactants. The usage of synthetic surfactants introduces new species of recalcitrant pollutants into the environment and leads to undesired results when a wrong selection of surfactants is made. Substituting synthetic surfactants with biosurfactants resolves these drawbacks, thus interest has been intensified in biosurfactant applications in a wide range of industries hitherto considered as experimental fields. This review, therefore, intends to offer an overview of diverse applications in which biosurfactants have been found to be useful, with emphases on petroleum biotechnology, environmental remediation, and the agriculture sector. The application of biosurfactants in these settings would lead to industrial growth and environmental sustainability.

Protective effects of di- and tri-peptides containing proline, glycine, and leucine on liver enzymology and histopathology of diabetic mice

Context Small peptides as multifunctional biomolecules can prevent the metabolic disorders such as diabetes. Objective The purpose of this study was to investigate the effects of small peptides on the enzymes and histopathology of the liver in mice exposed to diabetes. Methods Di- and tri-peptides containing proline, glycine, and leucine were produced by solid phase peptide synthesis (SPPS) protocol. The effects of produced peptides as well as carnosine (Ala-His) and glutathione (Glu-Cys-Gly) were evaluated on hepatic enzymes activity by enzymatic method and histopathology of liver using hematoxylin and eosin and TUNEL staining to assess histologic changes and apoptosis in diabetes induced by multiple low doses of streptozotocin (MLDS). Results The Ala-His, Leu-Gly and Pro-Gly-Pro peptides had the higher protective effects against the effects of diabetes on the enzymes and histologic changes of liver in mice. Conclusion These peptides can be raised as considerable pharmaceutical preventive agent against diabetes development.

Production, characterization, and applications of bioemulsifiers (BE) and biosurfactants (BS) produced by Acinetobacter spp.: A review.

Bioemulsifiers (BE) and biosurfactants (BS) are considered as multifunctional biomolecules of 21st century because of their functional abilities and eco-friendly properties. They are produced by various microorganisms under versatile and extreme environmental conditions. They have tremendous applications in various industries such as petroleum, food, medicine, pharmaceutical, chemical, paper & pulp, textile, and cosmetics. Currently, they are also considered as "green molecules" because of their wide applications in bioremediation of soil. Their importance has been increasing day by day in the global market as they are the natural resources with high-aggregate value. Although, there are numerous reports on BE and BS production by different bacteria, Acinetobacter spp. acquired special attention among all. This is because it is the earliest member known for the production of bioemulsifier. Emulsan and Alasan are the best examples of the commercially used BE produced by Acinetobacter spp. These BE are mainly used in microbial enhanced oil recovery and biodegradation of toxic compounds. This review is focused on BE and BS produced by Acinetobacter spp., their characterization and applications in different fields. This is the first review on genus Acinetobacter which defines independently about different types of BE and BS produced by it. It will also address the need of exploration of these molecules from various sources and their applications for the benefit of mankind and sustainable environment.

Transcriptomic changes under stress conditions with special reference to glutathione contents

Plants are sessile organisms. They have to endure the environmental catastrophe throughout their life cycle. To survive in these hostile surroundings, plants posses an efficient and fine-tuned defense mechanism. This is a well established fact that various defense molecules viz. salicylic acid (SA), jasmonic acid, abscisic acid (ABA), ethylene (ET) and so on, are working in a synergistic as well as antagonistic fashion to establish and activate the effective defense mechanism. Interestingly, glutathione is gaining a gradual importance in this complex scenario. This multifunctional biomolecule exists in two forms. The reduced form, viz. GSH, is primarily present at millimolar concentrations in various plant tissues as compared to its oxidized form, glutathione disulfide, GSSG. Proteo-genomics analysis confirmed that GSH plays a vital role in plant resistance against biotic and abiotic stresses by stimulating various defense genes and proteins. In recent times, it has been reported that modulation of GSH contents transmits information through diverse signaling mechanisms. GSH also modulates various stresses and defense related genes by interacting with ABA and ET in response to abiotic stress conditions. However, there are still many unanswered questions about the intricate molecular mechanism of GSH’s contribution in plant defense. With these backgrounds, presently we primarily discussed the transcriptomic changes under stress conditions in Arabidopsis thaliana at altered GSH contents. Transcriptomic profiling of phytoalexin-deficient mutant (pad2.1), a GSH depleted A. thaliana mutant, in response to combined cold and osmotic stress treatment, was compared to that of A. thaliana ecotype Col-0, the wild type, with a view to identify the genes altered under changed GSH conditions to combat stress. It was evident from these datasets that the transcript level responses of pad2.1 to this treatment were massive. Again, analysis of combined cold and osmotic stress treated other mutants of Arabidopsis transcriptome was performed to elucidate the crosstalk between the ABA, ET and GSH. Results revealed the differential regulation of about 2313 and 4131 transcripts in A. thaliana mutants viz. ethylene insensitive (ein2) and ABA deficient 1(aba1.6) respectively. Together, present findings elucidate an active interplay of GSH with SA, ET, and ABA to combat environmental stress conditions in planta.