Addition Of Multi-enzyme Research Articles

Effect of supplementation multi-enzyme on productive performance of two strains of Japanese quail

Five levels of multi-enzyme (Roxazyme) 0, 0.010, 0.020, 0.030 and 0.040% from total ration, and two strains of grower Japanese quail (Coturnix coturnix japonica ), desert and white color strain, and the interaction, use in this study. 640 birds of quail of two strains distributed to 5 experimental treatments, in each one 64 birds, and then divided to 4 replicates, with 16 birds in it. The results assert significant differences (p≤ 0.05) for strain, multi-enzyme addition and the interaction between them on body weight, weight gain, feed conversion coefficient, water conversion coefficient, carcass weight, dressing percentage, edible giblets percentage, breast percentage, Globulin on blood serum. The results of interaction between strain and multi-enzyme addition also show significant events (p≤ 0.05) on feed intake and water intake. Finally, Desert quail strain recorded the best net revenue (Iraqi Dinar/Kg live weight) (Iraqi Dinar/Kg carcass), the treatment of multi-enzyme addition 0.030% (T4) as well as registered heist net revenue (Iraqi Dinar/Kg live weight) (Iraqi Dinar/Kg carcass) and interaction between these treatments also show up best net revenue (Iraqi Dinar/Kg live weight) (Iraqi Dinar/Kg carcass). No significant effect in all other traits in this paper.

Influence of multienzyme additives and humic substances on the structure of the glandular part of the stomach of broiler chickens

Purpose of research was to study the effect of enzyme supplements and humic substances on the histostructure of the glandular part of the stomach of broiler chickens.Materials and methods. The experiment lasted 49 days. In the first 4 weeks, the level of metabolic energy in the diet was 10 kcal per 100 g of feed below the recommended level, then until the end of the experiment it was 5 kcal lower. Group 1 received the main diet, balanced in all respects. Group 2 received the enzyme preparation «Kemzaym» 1 g/kg feed. It contained beta-gluconase, cellulase, pentosanase, protease, lipase and alpha-amylase. Group 3 received sodium humate 1 g/kg of feed. Group 4 received both «Kemzyme» (1 g/kg) and sodium humate (1 g/kg) at the same time. Slaughter of chickens was at 28 days and 49 days of age. Samples of the glandular part of the stomach were taken from 5 chickens from the group.Results. The study of the histological picture showed that up to 49 days of age, the development and formation of the structures of the wall of the glandular stomach occurs. This is expressed in an increase in the thickness of the organ wall, mainly due to the submucosal layer. The use of «Kemzaym» continuously during the entire cultivation leads to a decrease in the thickness of the entire wall of the organ and inhibition of the development of the submucosa. There is also an irritating effect of the drug on the mucous membrane. In the first period, this is expressed in a decrease in the height of the mucosa, foci of violation of the integrity of the epithelium, atrophy of the glands of the mucosa and lymphocytic infiltration of the lamina propria are observed. In the second period, this is expressed in an increase in the thickness of the mucosa in the absence of destructive changes in its histological picture. Such a reaction of the structures can be considered as an adaptation of the stomach wall. The use of sodium humate in the first period leads to an increase in the thickness of the submucosa. No negative effect on the structures of the mucosa was found during this period. In the second period, this additive causes a decrease in the thickness of the submucosa. During this period, an increase in the layer of the mucous membrane is observed without destructive changes in its structure. The combined use of sodium humate and the enzyme complex does not have a positive effect on the wall of the glandular stomach.Conclusion. Studies have shown that long-term continuous use of enzyme supplements leads to a decrease in the production of the stomach's own enzymes. The use of sodium humate has a positive effect only in the first period, which indicates that the body is getting used to this additive.

Farklı Seviyelerde Aspir (Carthamus tinctorius L.) Küspesi İçeren Bıldırcın Rasyonlarına Enzim İlavesinin Performans, Yumurta Kalitesi ve Serum Parametrelerine Etkisi

This study was carried out to determine the effects of 0 and 1000 mg/kg multi-enzyme addition to the diets containing different levels (0, 5, 10 and 20%) of safflower seed meal on performance, egg quality and serum parameters in laying Japanese quails. 160 female Japanese quail (Coturnix coturnix Japonica) at 8 weeks of age were used in the study. It was carried out for 84 days with 5 replications in a quail cage with 4 Japanese quails in each of 8 treatment groups consisting of enzyme addition to diets containing different levels of safflower seed meal. In laying quail, the effect of enzyme addition to the diets containing different levels of safflower meal on body weight, body weight change, egg production and feed conversion ratio at the end of the experiment was insignificant. While feed intake was not affected by the addition of enzyme to the diet, feed intake in the group containing 20% safflower meal was significantly higher than the group containing 5% safflower meal, and this group was similar to the other groups. Egg weight and eggshell breaking strength were not significantly affected by the treatments. While the effect of dietary safflower meal levels on egg mass and eggshell thickness was significant, the effect of enzyme addition on eggshell ratio was significant. The effects of treatments on albumen index, yolk index and Haugh unit were insignificant. While a* and b* values in egg yolk colour properties were not significantly affected by the treatments, a significant decrease was observed in L* value with the addition of enzyme to the diet. The effects of treatments on serum glucose, cholesterol, AST, ALT, calcium, and phosphorus concentrations were insignificant. According to the test results, it can be said that 20% safflower meal can be used in laying quail diets without the need for enzyme addition.

Dietary Brewer Grain Meal with Multienzymes Supplementation Affects Growth Performance, Gut Health, and Antioxidative Status of Weaning Pigs

We conducted a 28-day feeding study on 80 weaning pigs [(Landrace × Large White) × Duroc] to determine the effects of dietary inclusion with brewer’s grain meal (BGM) and multienzymes on their growth, intestinal health, and antioxidative status. Piglets were grouped by sex and initial BW and assigned to 20 pens with four pigs each. Treatments were a corn–soybean meal-based diet with either 0.1% multienzyme addition (PC) or without (NC), and two BGM compositions fortified with 0.1% multienzyme: 10% (BGM10) and 20% (BGM20). The overall body weight, average daily weight gain, and weight gain:feed ratio were significantly greater in pigs fed BGM20 than those fed the NC diet (p < 0.05). Moreover, the BGM diets significantly increased the digestibility of total ash and ether extract, glucose, total protein, immunoglobulin A, total antioxidant capacity, superoxide dismutase, heart and small intestine weights, villus height: crypt depth ratio (VH/CD), and Lactobacillus spp. count compared with the NC diet (p < 0.05). The diarrheal rate, blood urea nitrogen, malondialdehyde, duodenal crypt depth, and Salmonella spp. count were reduced in pigs fed the BGM-supplemented diet than those fed the NC diet (p < 0.05). The diarrheal rate (p = 0.010), ether extract digestibility (p = 0.044), total protein (p = 0.044), and duodenal villus height and VH/CD (p = 0.003 and p = 0.002, respectively) decreased quadratically with the increase in BGM supplementation. Overall, diets containing up to 20% BGM with multienzyme addition improved the nutrient utilization and intestinal health in weaning pigs by suppressing pathogenic bacterial growth without compromising the overall growth of the pigs.

Multi-Enzyme Supplementation Modifies the Gut Microbiome and Metabolome in Breeding Hens.

Laying and reproductive performance, egg quality, and disease resistance of hens decrease during the late laying period. Exogenous enzymes promote nutrient digestibility and utilization and improve the intestinal environment. However, the specific regulation of the gut microbiome and metabolome by exogenous enzymes remains unelucidated. This study was conducted to evaluate effects of dietary multi-enzyme supplementation on egg and reproductive performance, egg quality, ileum microbiome, and metabolome of breeders. Here, 224 Hy-Line Brown breeding hens (55 weeks old) were randomly allocated to two groups: dietary controls fed basal diet (DC), and test hens fed 0.2 g/kg corn enzyme diet (CE). Serum levels of total protein, globulin, immunoglobulin Y, and antibodies against the Newcastle disease virus and avian influenza H9 strain were significantly increased (p < 0.05). Egg albumen height, Haugh unit, and fertilization and hatching rates were also significantly increased (p < 0.05) in the CE-fed group. 16S rRNA sequence analysis showed that CE strongly affected both α- and β-diversity of the ileal microbiota. LEfSe analysis revealed that the potentially beneficial genera Lactobacillus, Enterococcus, Faecalicoccus, and Streptococcus were enriched as biomarkers in the CE-fed group. Microbial functional analysis revealed that the functional genes associated with harmful-substance biodegradation was significantly increased in the CE-fed group. Additionally, Spearman correlation analysis indicated that changes in microbial genera were correlated with differential metabolites. In summary, dietary multi-enzyme addition can improve egg quality, humoral immunity, and reproductive performance and regulate the intestinal microbiome and metabolome in breeders. Therefore, multi-enzymes could be used as feed additive to extend breeder service life.

Enhancing Growth Performance, Organ Development, Meat Quality, and Bone Mineralisation of Broiler Chickens through Multi-Enzyme Super-Dosing in Reduced Energy Diets.

Simple SummaryThe global population is expected to rise from 7.2 billion as of 2019 to 9.7 billion in 2050, putting pressure on farmers to increase production capacity to ensure food security whilst simultaneously improving food sustainability. Poultry is an important meat, as chickens have high feed efficiencies and short production cycles, making it an affordable, nutritious source of protein. Strategies to improve the production performance of broilers will require significant research; one nutritional strategy is improving the efficiency of feed utilization via the addition of exogenous enzymes into diets. This study aimed to identify the optimal multienzyme, Natuzyme, dose rate at three energy levels based on production performance, organ development, meat quality, and bone mineralization in broiler chickens. Results revealed that all dose rates of Natuzyme were able to mitigate the negative effect of energy reductions. Organ development and meat quality remained consistent across treatment groups, except for the gizzard and meat moisture content, which were affected by super-dosing Natuzyme. Bone mineralization was restored with the inclusion of Natuzyme. In conclusion, super-dosing Natuzyme in reduced energy diets at a dose rate of 700 g/t can improve performance parameters and thus profitability for producers and can improve the sustainability of production.This study identified the optimal multi-enzyme dose rate at three energy levels based on the production performance of broiler chickens. A 42-day grow out trial was conducted using 576 day-old mixed-sex ROSS308 broiler chickens in a 3 × 4 factorial arrangement in a completely randomized design. Diets consisting of three metabolizable energy (ME) levels: standard energy (STD), 150 kcal/kg energy reduction (STD-150), and 200 kcal/kg energy reduction (STD-200), were cross factored with four multi-enzyme inclusion levels (0, 350, 700, and 1000 g/ton). The average daily feed intake and feed conversion ratio increased linearly (p < 0.001) as the dietary ME was reduced, and the multi-enzyme addition improved the feed conversion ratio (p < 0.05) and mitigated the negative effect of the reduced energy diets (RED) on feed intake and feed conversion ratios. Carcass composition, organ weights, and meat quality were not affected by the experimental diets. The RED decreased abdominal fat weight (p < 0.05). Total ash, calcium, and phosphorous contents of the tibia bone were improved (p < 0.04) when the RED were supplemented with the multi-enzyme. Super-dosing multi-enzymes in RED mitigates the negative effect of ME reduction on growth performance while maintaining organ development and meat quality and improving bone mineral content.

THE RESPONSES OF GROWTH PERFORMANCE, DIGESTIBILITY AND BLOOD BIOCHEMISTRY OF CHICKENS TO THE DOSE AND ADMINISTRATION METHOD OF ENZYMES

The aim of this study was to investigate the responses of growth performance, digestibility and blood biochemistry to the dose and administration method of enzymes. A total of 150 unsexed 1-d-old Arbor Acres broiler chickens were divided equally among 5 dietary treatments with 6 replicates per treatment and five chickens each. All experimental groups were fed the same basal diet and given 5 multienzyme treatments: The 1st group, the control group, did not receive multienzyme supplementations. The 2nd, 3rd, 4th and 5th groups were given multienzyme in water at 100 and 150% of the recommended dosage in drinking water given either continuously or intermittently methods, respectively. In the continuous method, the multienzyme is added to the water over the day. While, in intermittently method, the multienzyme is added to the water over the day followed by day off during the 1st through the 35th days of age. The addition of multienzyme either at 100 or 150% to water improves the growth performance and nutrient digestibility i.e. dry matter, crude protein and ether extract of broiler chickens compared with the control. However, 100% of multienzyme resulted in the best growth performance than that 150% multienzyme group. Intermittently administration exhibited significantly better growth performance and nutrient digestibility i.e. dry matter and NFE than those given multienzyme with continuously administration way. There were no significant effects of the multienzymes dose and the administration methods on carcass characteristics and blood biochemical constituentsexcepttriglycerides and creatinine. In conclusion, broilers received multienzymes at 100% intermittently in water exhibited significantly higher growth rate and significantly the best FCR. Production index was also the best of this group.

Bıldırcın Rasyonlarına Multi-Enzim İlavesinin Performansa, Yumurta Kalitesine ve Serum Parametrelerine Etkisi

Bu çalışma bıldırcın rasyonlarına farklı seviyelerde multi-enzim ilavesinin yumurtlayan bıldırcınların performans, yumurta iç ve dış kalite parametreleri ile bazı serum parametreleri üzerine etkilerinin değerlendirilmesi için yürütülmüştür. Denemede 10 haftalık yaşta toplam 96 adet Japon bıldırcını dört tekerrürlü altı muamele grubuna ve her bir alt grupta dört bıldırcın olacak şekilde rastgele dağıtılmıştır. Mısır-Soya fasulyesi küspesine dayalı bazal rasyona 100, 500, 1000, 1500 ve 2000 mg/kg multi-enzim ilave edilerek toplamda 6 adet rasyon hazırlanmıştır. Deneme sonuçlarına göre rasyona farklı seviyelerde multi-enzim ilavesinin canlı ağırlık değişimi, yumurta verimi, yumurta ağırlığı, yumurta kitlesi, yem tüketimi ve yemden yararlanma oranına etkisi istatistiki olarak önemsiz olmuştur. Buna ilaveten, muamelelerin kabuk kalınlığı haricinde yumurta iç ve dış kalite parametrelerine etkisi de önemsiz olmuştur. En yüksek yumurta kabuk kalınlığı 1000 mg/kg multi-enzim seviyesinde elde edilmiştir. Serum parametrelerinden glukoz, kreatinin ve kolesterol etkilenmezken AST 100 mg/kg seviyesinde ve albümin, globülin, total protein, kalsiyum ve fosfor konsantrasyonları 2000 mg/kg seviyesinde multi-enzim ilavesinde en yüksek değere ulaşmıştır. Bu sonuçlara göre rasyona yüksek seviyede (2000 mg/kg) multi-enzim ilavesinin bıldırcınların serum parametrelerini iyileştirdiğini, ancak serum parametrelerindeki bu iyileşmenin bıldırcınların performans ve yumurta kalitesine yansımadığı görülmüştür.

Effect of addition of multi-enzyme and varying levels of toasted African yam bean seed meal on carcass characteristics and internal organs of broiler starter

African yam bean seed is one of the underutilized legumes and a prospective feed ingredient in poultry diet. This study was carried out to examine carcass characteristics and internal organs of broiler starter fed partial replacement of toasted African yam bean seed meal supplemented with multi-enzyme (TAYBSM+E). There were five treatments: treatment one (T1) served as control without TAYBSM+E while T2, T3, T4 and T5 contained 20%, 40%, 60% and 80% TAYBSM+E, respectively and supplied experimental broiler birds for four weeks. Each treatment was randomly assigned to one of the five experimental diets in a completely randomized designed. Two hundred birds were allotted to five treatments replicated four times with 10 birds per replicate. At four weeks of age, experimental birds were starved for 12 hours; one bird was sacrificed per replicate (four per treatments), defeathered and cut into different parts. Carcass cuts and vital organs were carefully collected and weighed with sensitive scale. All parameters in the carcass cuts and internal organs weight examined were not statistically (P&gt;0.05) influenced except intestine, lungs and thigh that significantly (P&lt;0.05) different. It is concluded that toasted African yam bean seed meal supplemented with multi-enzyme is a good source of protein that can be used in broiler feeds safely up to 80% inclusion level to give satisfactory results.&#x0D;

Growth performance, nutrient digestibility, and fecal microbial composition of weaned pigs fed multi-enzyme supplemented diets.

A study determined the effects of supplementing corn-based diets for weaned pigs with multi-enzymes on growth performance, apparent total tract digestibility (ATTD) of nutrients, fecal score, and fecal microbial composition. A total of 132 pigs (initial body weight = 7.23 kg) that had been weaned at 21 d of age and fed a drug-free nursery diet for 7 d were housed in 33 pens of 4 barrows or gilts, blocked by body weight and gender, and fed 3 experimental diets at 11 pens per diet. The diets were corn-based diet without or with multi-enzyme A or B. Multi-enzyme A supplied 4,000 U of xylanase, 150 U of β-glucanase, 3,500 U of protease, and 1,500 U of amylase per kilogram of diet. Multi-enzyme B was the same as multi-enzyme A except that it supplied amylase at 150 U/kg, and that its source of amylase was different from that of multi-enzyme A. All diets contained phytase at 1,000 U/kg. The diets were fed for 35 d in 2 phases; phase 1 for the first 14 d and phase 2 for the last 21 d of the trial. Fecal score was determined daily during the first 7 d of the trial. Fecal samples were collected from rectum of 1 pig per pen on days 2, 7, 14, and 35 of the trial for determining bacterial composition. Also, fresh fecal samples were collected from each pen on days 41 and 42 to determine ATTD of nutrients. Multi-enzyme B increased (P < 0.05) average daily gain (ADG) for phases 1 and 2. For the overall study period, multi-enzyme B increased (P < 0.05) ADG from 262 to 313 g, and average daily feed intake (ADFI) from 419 to 504 g. Multi-enzyme A increased (P < 0.05) overall ADG from 262 to 290 g, but did not affect ADFI. Multi-enzyme A or B did not affect ATTD of gross energy, but increased (P < 0.05) the ATTD of ether extract from 30% to 36% or 37%, respectively. Multi-enzyme A did not affect fecal score; however, multi-enzyme B tended to decrease (P = 0.09) fecal score, implying that it tended to decrease diarrhea. Firmicutes were the most abundant phylum of fecal bacteria (its relative abundance ranged from 58% to 72%). Bacteroidetes and Actinobacteria were the 2nd and 3rd most abundant phyla of fecal bacteria. Neither multi-enzyme affected fecal bacterial composition. In conclusion, the addition of multi-enzyme A or B to phytase-supplemented corn-based diet for weaned pigs can improve their growth performance and fat digestibility. However, multi-enzyme B was more effective than multi-enzyme A in terms of improving the growth performance of weaned pigs fed corn-based diet.

Effect of Different Dietary Inclusion Levels of Sunflower Meal and Multi-Enzyme Supplementation on Performance, Meat Yield, Ileum Histomorphology, and Pancreatic Enzyme Activities in Growing Quails.

Simple SummaryIn the poultry industry, where the cost of feed constitutes most of the expenses, sudden changes in feed prices make it a challenge for nutritionists to maintain the yield and health of animals while managing the diet costs. It is the first solution that comes to mind to use inexpensive raw materials while preparing diets in practical conditions. In addition, feeds used in diets should be digestible by poultry. In the poultry digestive system, feeds such as sunflower meals are difficult to digest due to containing high cellulose because there is no cellulase secretion in the digestive system. The use of multi-enzyme which enhanced the digestibility of nutrients and decreased antinutritional compounds such as cellulose and nonstarch polysaccharides in diets with sunflower meal can positively affect performance and the profitability of the growers.This study aimed to investigate the effect of dietary sunflower meal (SFM) and multi-enzyme levels on performance, carcass traits, intestinal histomorphology and pancreatic enzyme production in quails. Three hundred and twenty, 1-day-old quail chicks were divided into 8 groups with 4 replicates consisting of 10 birds each in the group. The experiment was randomized design consisting of a 4 × 2 factorial arrangement, with four levels of SFM (0%, 10%, 15%, or 20%) and two levels of multi-enzyme (0.0 or 1.0 g/kg) inclusion in the diet. The body weight, body weight gain, and feed conversion ratio were negatively influenced by the 15% and 20% SFM (p < 0.01) but were not affected by the 10% SFM for 6 week age. The relative gizzard (p < 0.05) weights significantly increased with 20% SFM, but the relative breast weight decreased (p < 0.01). The relative liver weight increased by the addition of enzymes in the diet (p < 0.05). The villus width (p < 0.01) and villus surface area (p < 0.05) of ileum increased linearly with SFM, whereas the villus height (p < 0.01), villus height: crypt depth (p < 0.01) and tunica muscularis thickness (p < 0.01) decreased linearly with SFM. Consequently, it is possible to say that the birds with the least absorptive same weight are the most efficient. The addition of multi-enzyme increased villus height and crypt depth but decreased tunica muscularis thickness of ileum (p < 0.01). Chymotrypsin activity in the pancreas decreased linearly with SFM (p < 0.01). Amylase activity in the pancreas decreased significantly with the addition of the multi-enzyme (p < 0.05). As a result of the study, SFM can be used at a 10% level in growing quail diets with beneficial effects on the absorption surface area. The effects of enzyme supplementation on parameters measured were less pronounced than the SFM inclusion level that higher villus height and lower tunica muscularis thickness were determined in multi-enzyme-fed birds compared to those untreated counterparts.

PENGARUH PEMBERIAN TEPUNG KIAMBANG (Salvinia molesta) DENGAN ADITIF MULTIENZIM DALAM RANSUM TERHADAP PERFORMANS ITIK TEGAL

ABSTRACT&#x0D; The research aims to determine the influence of using Salvinia molesta with multienzyme additive in rations of tegal duck performans. This research uses 72 Tegal female ducks 22 weeks old. Feed ingredients used in the reasearch consisted of corn, soybean meal, oil, bran, fish meal, premix, methionine, lysine, Salvinia molesta and multienzyme additives. The reasearch was conducted with Completely Randomized Design with 6 treatments and 4 replications of the study. Each experimental unit consists of 3 ducks. Ransum namely: T0: feed without Salvinia molesta and enzyme; T1: feed without Salvinia molesta + multienzim; T2: feed with Salvinia molesta. 5% + without multienzymes; T3: feed with Salvinia molesta. 5% + multienzim; T4: feed with Salvinia molesta. 7,5% + multienzim; T5: feed with Salvinia molesta. 10% + multienzim. The result showed no significant treatment (P&gt; 0,05) to consumtion of ration, feed conversion and Tegal duck eeg weightbut have an effect on hen day production (P

158 Growth performance of weaned pigs fed multi-enzyme supplemented corn-soybean meal-based diets – two semi-commercial studies

Abstract Two studies were conducted with same set-up at a commercial facility to determine the effects of supplementing corn-SBM-based diets with or without a multi-enzyme on performance of nursery pigs. A total of 240 piglets (per trial) weaned at 25 d of age were randomly assigned to the 12 pens (10 barrows and 10 gilts per pen) pre-assigned with one of the experimental diets: a drug-free control diet containing 3000 ppm Zn and 1000 FTU phytase/kg with or without a multi-enzyme (4,000 U of xylanase, 150 U of amylase, 150 U of β-glucanase and 3000 U of protease/kg feed, n = 6). The diets were fed for 6 wks in 2 growth phases (phase 1: 0–2 weeks; phase 2: 3–6 weeks). Feed intake (ADFI), body weight (BW), body weight gain (ADG) were recorded by phase. Data were analysed by MIXED procedure of SAS (9.4) and significance was declared at P &lt; 0.05. In phase 1, fecal scores were visually assessed daily by pen, ranging from 1 to 5 (1 = hard; 2 = firm; 3 = soft; 4 = very soft; 5 = watery feces). The frequency of diarrhea by pen were calculated by counting pigs with a diarrhea score of 3 or greater. In both trials, ADG during the first phase was improved by 30 (trial 1) or 40 g (trial 2) vs. control (P &lt; 0.05). Final BW in piglets fed enzyme treatment was 1.4 (P &lt; 0.05) or 1.2 kg (P &gt; 0.05) heavier in trial 1 and 2, respectively vs. those fed the control diet. The ADFI was not affected by enzyme inclusion (P &gt; 0.05). In addition, during the 1st phase, improvement in fecal condition (average 1.96 vs. 2.27; P &lt; 0.05) and reduction in diarrhea incident (average 3.0 vs. 5.05%; P &lt; 0.05) were observed in both trials when multi-enzyme treatment was fed to the piglets. In conclusion, the addition of multi-enzyme for weaned pigs improved growth performance and health condition.

Les lapins nourris avec de l’amidon de manioc fermenté résidus II: enzyme supplementation

La probabilité d’augmenter le niveau de remplacement optimale du maïs avec de l’amidon de manioc fermenté d’origine microbienne (résidus MFCSR) avec multi-enzyme supplementation en régimes de lapin a été évalué dans un arrangement factoriel 2x3 à huit semaines au procès. Bien que le gain de poids total (GTT) et le ratio de conversion d’alimentation (FCR) a significativement (P

Bioethanol Production By Utilizing Cassava Peels Waste Through Enzymatic And Microbiological Hydrolysis

Cassava peels waste contains, cellulose which is quite high at 43.626%, this is a potential candidate as a raw for bioethanol production. The purpose of this study was to determine the performance of the enzymatic hydrolysis, microbiological (Effective microbe) and fermentation in cassava peel waste is known from the results of quantitative measurement of multiple ethanol parameters (DNS Test, pH, ethanol concentration). This research was carried out in stages, the first stage is hydrolysis with completely randomized design with single factor variation of the catalyst, consisting of three levels ie cellulase enzymes, multienzyme and effective microbial EM4. The second stage is fermentation with factorial randomized block design, consisting of three groups of variations of catalyst, and has two factors: variations of fermipan levels 1, 2, 3% and the duration of fermentation, 2,4,6 days. The parameters in the test is a reducing sugar, pH and concentration of ethanol. The results showed that variation of hydrolysis treatment, fermentation time, and fermipan levels has real effect on the fermentation process. On average the highest ethanol content obtained from the treatment with multienzyme addition, with the addition of 2% fermipan levels and on the 2nd day of fermentation that is equal to 3.76%.

Effect of Diet Containing Gracilaria Sp. Waste and Multi-Enzyme Additives on Blood Lipid Profile of Local Duck

This study was aimed to evaluate the effect of diet containing waste of seaweed Gracilaria sp. on local duck blood lipid profiles including cholesterol, triglycerides, low density lipoprotein (LDL), and high density lipoprotein (HDL). The material in this study were 72 female Tegal ducks aged 22 weeks with 1,318 ± 121 gaverage body weight, diets containing Gracilaria sp. waste (GW) with 18% protein and 2900 kcal/kg metabolic energy and 150 g/ton feed commercial multi-enzyme (ME). Data were subject to Completely Randomized Design with 6 treatments, (T0) Basal Diets, (T0+) Basal Diets with ME, (T1) Diets with 10% GW, (T1+) Diets with 10% GW + ME, (T2+) Diets with 12.5 % GW + ME, and (T3+) Diets with 15% GW + ME. The treatment was givenfor 12 weeks and blood sample was taken onthe last week. The serum was separated and analyzed for blood lipid profiles using CHOD-PAP. Results showed that diet with 12.5%GW plus multi-enzyme additives significantly (P&lt;0.05) affected blood lipid profiles of local duck, tended to lower triglycerides and LDL Cholesterol, but increase blood HDL levels.

Effect of Diet Containing Gracilaria Sp. Waste and Multi-Enzyme Additives on Blood Lipid Profile of Local Duck

This study was aimed to evaluate the effect of diet containing waste of seaweed Gracilaria sp. on local duck blood lipid profiles including cholesterol, triglycerides, low density lipoprotein (LDL), and high density lipoprotein (HDL). The material in this study were 72 female Tegal ducks aged 22 weeks with 1,318 ± 121 gaverage body weight, diets containing Gracilaria sp. waste (GW) with 18% protein and 2900 kcal/kg metabolic energy and 150 g/ton feed commercial multi-enzyme (ME). Data were subject to Completely Randomized Design with 6 treatments, (T0) Basal Diets, (T0+) Basal Diets with ME, (T1) Diets with 10% GW, (T1+) Diets with 10% GW + ME, (T2+) Diets with 12.5 % GW + ME, and (T3+) Diets with 15% GW + ME. The treatment was givenfor 12 weeks and blood sample was taken onthe last week. The serum was separated and analyzed for blood lipid profiles using CHOD-PAP. Results showed that diet with 12.5%GW plus multi-enzyme additives significantly (P&lt;0.05) affected blood lipid profiles of local duck, tended to lower triglycerides and LDL Cholesterol, but increase blood HDL levels.

Performance and nutrient digestibility in broiler chicks as influenced by multienzyme addition to starter diets containing palm kernel meal

This study was conducted to investigate the performance and nutrient digestibility in broiler chicks as influenced by multi-enzyme (Hemicell +Roxazyme G) addition to starter diets containing palm kernel meal. Nine experimental diets were formulated such that diet 1 which served as control contained 0 % PKM without enzyme supplementation. Diet 2, 3, 4 and 5 contained 10, 20, 30 and 40 % PKM levels respectively with multi-enzyme supplementation while diets 6, 7, 8 and 9 contained 10, 20, 30 and 40 % PKM inclusion levels respectively without multi-enzyme supplementation. Five hundred and forty (540) day old hybro broilers of mixed sex in ratio (1: 1) were randomly assigned to nine diets in a completely randomized design. Each treatment was replicated thrice with 20 birds per replicate. The experiment lasted 35 days. The results showed that nutrient digestibility in the control and 10 % PKM with enzyme supplementation were similar but were significantly (P&lt;0.05) higher than other PKM diets with or without supplementation. There was significant (P&lt;0.05) improvement in body weight and body weight gain and reduce feed intake with supplementation. Birds fed with 20 % PKM with enzyme showed similarity with control birds in all the performance parameters measured. Enzyme addition significantly (P&lt;0.05) reduced cost of feed consumed at 30 % level of inclusion with PKM while cost per kilogram weight gain and cost of production were lower at 20 % PKM level.

Effects of sprouted and nonsprouted wheat and barley with and without enzyme on intestinal morphometry of broiler chickens

This experiment was conducted to investigate the effects of sprouted and nonsprouted wheat and barley with and without enzyme on the histomorphology of small intestinal mucosa. Three hundred and thirty-six 1-day-old male broiler chicks (Ross 308) were divided randomly into seven treatments and four replications (12 chicks in each replicate). The experimental treatments were as follows: (1) corn-based diet (control), (2) wheat-based diet + 0.1 % enzyme (a multienzyme product), (3) sprouted wheat-based diet + 0.1 % enzyme, (4) sprouted wheat-based diet without enzyme, (5) barley-based diet + 0.1 % enzyme, (6) sprouted barley-based diet + 0.1 % enzyme, and (7) sprouted barley-based diet without enzyme. For enteric morphometric analysis, the small intestines of the four broilers for each treatment were collected at 42 days. The traits evaluated were villus length, villus width, and crypt depth. The addition of multienzyme to wheat- and barley-based diets and the use of sprouted wheat in the diet increased the villus height in the ileum compared with the control. The addition of multienzyme to wheat- and barley-based diets and the use of sprouted wheat in the diet increased (P < 0.05) the villus height in the ileum compared with the control. The villus crypt depth was significantly decreased for sprouted barley supplementations compared with the control. The sprouted wheat with enzyme supplementation increased the villus length/crypt depth ratio compared with the control. According to results of this experiment, it can be concluded that wheat and barley with multienzyme or sprouting wheat and barley can be used as replacement grains for corn in broiler production.

Estimation of standardised ileal threonine equivalency values of a multi-enzyme and its effects on broiler chick’s performance

An experiment was conducted to estimate standardised ileal threonine (SID Thr) equivalency of a commercial multi-enzyme and its effects on broiler chick’s performance. The 10 treatments included a basal diet containing 0.56% and 0.46% SID Thr in starter and grower periods; treatments 2 to 5 were supplemented with graded levels (0.07%, 0.14%, 0.21% and 0.28%) of L-threonine and treatments 6 to 10 were basal diet supplemented with 0.1, 0.2, 0.3, 0.4 and 0.5 g/kg of multi-enzyme. Supplementing diets with multi-enzyme significantly (P<0.01) improved the feed conversion ratio at 28 and 42 d of age. Multi-enzyme addition linearly increased body weight gain (P<0.05) at 28 d of age and the immune response (P<0.05) at 36 d of age. Breast, thigh and abdominal fat relative weights were not affected by supplementing the multi-enzyme. Nonlinear and linear equations were generated for the multi-enzyme and graded levels of threonine. Based on an assessment for the r2 and P values of the equation, body weight gain, immune response and feed conversion ratio were sensitive measurements of responses to multi-enzyme addition. The nonlinear or linear response equation with the higher r2 values for added L-threonine and the equation for added multi-enzyme were set to be equal and were solved. The body weight gain and feed conversion ratio responses from 0.5 g multi-enzyme per kg of diet at 28 and 42 d of age was therefore equal to 0.08%, 0.05%, 0.16% and 0.07% SID Thr, respectively, and the immune response from 0.5 g multi-enzyme per kg of diet was equal to 0.14% SID Thr.