Multisite Field Experiment Research Articles

Modeling the Effect of Milk Vetch–Rice Rotation on N Runoff Loss in the Middle and Lower Reaches of the Yangtze River

The winter planting of green manure (GM) is widely used in South China to reduce chemical nitrogen (N) fertilizer use, improve soil fertility, and maintain rice yields, but its effect on N runoff loss in paddy fields remains unclear. This study combines multi-site field experiments with a process model (WHCNS-Rice) to assess how GM with reduced N fertilizer impacts N runoff loss and its forms in the Yangtze River’s middle and lower reaches, considering different rainfall years. The network field experiments included four treatments: conventional fertilization (FR), conventional fertilization plus straw return (FRS), GM with a 40% N reduction (MR), and GM-straw combined return with a 40% N reduction (MRS). Monitoring the results showed that compared to the winter fallow treatment, the GM treatments reduced the peak and average total N (TN) concentrations by 11.1–57.9% (average 26.9%) and 17.1–27.3% (average 22.3%), respectively. The TN runoff loss under the GM treatment decreased by 3.50–10.61 kg N ha−1 (22.5–42.1%). GM primarily reduced the runoff loss of dissolved inorganic N (DIN), with reductions at different sites ranging from 0.22 to 9.66 kg N ha−1 (8.4–43.4%), indicating GM effectively decreases N runoff by reducing DIN. Model simulations of ponding water depth, runoff, TN concentration in surface water, and TN loss in paddy fields produced the consistency indices and simulation efficiencies of 0.738–0.985, 0.737–0.986, 0.912–0.986, and 0.674–0.972, respectively, indicating that the model can be used to evaluate water consumption and N runoff loss in the GM-paddy system. The simulations showed that GM with a 40% N fertilizer significantly reduced N runoff loss under all rainfall conditions, with the greatest reductions in wet years. Under wet, normal, and dry conditions, the GM treatments significantly reduced average TN loss by 0.37–5.53 kg N ha−1 (12.77–29.17%), 0.21–5.32 kg N ha−1 (9.95–24.51%), and 0.02–3.2 kg N ha−1 (1.78–23.19%), respectively, compared to the winter fallow treatment. These results indicate that the combination of GM and a 40% reduction in N fertilizer can significantly reduce N runoff loss from paddy fields, demonstrating good effectiveness under various rainfall conditions, making it a green production model worth promoting.

Invasive shrub removal may be more effective at reducing granivory than coating tree seeds with capsaicin

The utility of seed addition to promote tree regeneration can be greatly limited by animals that consume seeds. Moreover, given that restoration often occurs in forests where invasive shrubs are abundant, and evidence that invasive shrubs can increase granivory, it is important to explore whether methods for reducing granivory work equally well in invaded and uninvaded habitats. We used a multi-site field experiment spanning 160 days to explore whether coating seeds of Prunus serotina with capsaicin extract leads to reduced granivory in habitats with or without invasive shrubs (Rhamnus cathartica). Capsaicin-coated seeds were removed at a similar rate to uncoated seeds, but seeds in invaded plots had a 78.8% higher rate of removal compared to plots without invasive shrubs. Our findings suggest that managers seeking to encourage regeneration of native trees using direct seeding should consider invasive shrub removal as a top priority to limit the loss of seeds once sown.

Creating a resilient pedagogy: Character strengths intervention for aspiring educators

Teachers play a crucial role in guiding learners through life's challenges. They face educational and socio-economic shifts while striving to teach for a better future. Our study focuses on equipping future teachers, called pre-service teachers, with resilience-building resources during their teacher training program. A potential antecedent to resilience, which facilitates cognitive strategies and attentional processes, is cognitive flexibility. We first tested whether cognitive flexibility predicts resilience. Next, relying on the tenets of positive psychology, which studies resilience, we developed and tested the effectiveness of character strengths intervention in enhancing pre-service teachers' resilience and cognitive flexibility. An individual's belief in their ability to change personal resources could influence how an intervention manifests. Hence, we studied how mindset impacts the intervention's effect on cognitive flexibility and, thus, resilience. We adopted a multi-method approach, guided by Polk's theory of resilience, to test out objectives.Using a cross-sectional design, study one (n = 273) found that cognitive flexibility significantly predicted resilience. Study two (N = 193; nexp = 133, ncont = 60) was a multi-site field experiment. We found that intervention significantly enhanced resilience (experimental group M = 29.62, control group M = 28.33) and cognitive flexibility (experimental group M = 54.42, control group M = 52.01). Further, a growth mindset, was found to moderate the indirect effect of character strengths intervention on resilience via cognitive flexibility. The study contributes to theoretical and practical advancements in resilience. Taken together, the findings highlight the cognitive-affective-behavioural makeup of resilience and, importantly, the role of cognitive flexibility. The intervention can be seamlessly integrated into teacher training curricula for a resilient future.

Contaminated soil remediation with nano-FeS loaded lignin hydrogel: A novel strategy to produce safe rice grains while reducing cadmium in paddy field

Cadmium (Cd) contamination in agricultural soil has been an elevated concern due to the high health risks associated with the transfer through the soil-food chain, particularly in the case of rice. Recently, there has numerous researches on the use of nanoparticle-loaded materials for heavy metal-polluted soil remediation, resulting in favorable outcomes. However, there has been limited research focus on the field-scale application and recovery. This study was aimed to validate the Cd reduction effect of the nano-FeS loaded lignin hydrogel composites (FHC) in mildly polluted paddies, and to propose a field-scale application method. Hence, a multi-site field experiment was conducted in southern China. After the application for 94–103 days, the FHC exhibited a high integrity and elasticity, with a recovery rate of 91.90%. The single-round remediation led to decreases of 0.42–31.72% in soil Cd content and 1.52–49.11% in grain Cd content. Additionally, this remediation technique did not adversely impact rice production. Consequently, applying FHC in the field was demonstrated to be an innovative, efficient, and promising remediation technology. Simultaneously, a strategy was proposed for reducing Cd levels while cultivating rice in mildly polluted fields using the FHC.

Core bacteria and fungi in response to residue retention and their contribution to soil multifunctionality in maize agroecosystem

Core microbiome has been proven to play crucial roles in soil function. However, we still lack knowledge on how core microbiome responds to crop residue retention, and whether they contribute to this process. Consequently, we examined the effect of residue retention on soil core and non-core microbial communities in maize seedling, mature stage and freezing period based on a multi-site field experiment in Sanjiang Plain, Northeast China. Totally, 247 bacterial amplicon sequence variants (ASVs) and 109 fungal ASVs were identified as core microbiota. Both core and non-core bacterial/fungal community composition were significantly influenced by residue retention across all study sites. Especially, the core fungal community shifted towards a saprotroph-dominated community. Normalized stochastic ratio pattern revealed that that deterministic process dominated both core and non-core microbial community assembly processes. Residue retention enhanced the deterministic process of core microbial community assembly, while exhibited opposite effect on non-core microbial community. This study also revealed that soil fungi were more sensitive to residue retention than bacteria, with a larger proportion of core fungi were enriched or depleted by residue retention. In addition, residue retention complicated core bacterial co-occurrence network, while simplified fungal network. Our results pointed out both no reduction in microbial diversity or collapse in microbial network structure after repeated freezing-thawing cycles. The potential function of core microbiome was evaluated through random forest analysis and structural equation model, the results indicated core microbiome contributed more to multifunctionality than non-core microbiome. Overall, this study strengthened our understanding of soil core microbiome in response to residue retention, and highlighted their importance in maintaining soil multifunctionality.

The ecological footprint of outdoor activities: Factors affecting human-vectored seed dispersal on clothing

In the Anthropocene, humans are among the most abundant long-distance seed dispersal vectors globally, due to our increasing mobility and the growing global population. However, there are several knowledge gaps related to the process of human-vectored dispersal (HVD) on clothing. In a multi-site field experiment covering various habitat types in three countries of Central-Europe, we involved 88 volunteer participants and collected 251 HVD samples and 2008 subsamples from their socks and shoes. We analysed the number of diaspores and species in the samples. Specifically, we studied the effects of site characteristics (variables related to habitat types and season), vector characteristics (activity type, gender, clothing type, shoe type) and plant characteristics (species pool of the visited habitats and plant traits) on the number of diaspores and array of species dispersed. We assessed the habits of people that could be relevant for HVD with a questionnaire survey. A total of 35,935 diaspores of 229 plant taxa were identified from the samples, which indicates a huge potential of HVD in dispersing diaspores across habitats and regions. Most diaspores were recorded in grassland habitats, and more diaspores were dispersed during fieldwork than excursions. Clothing type also played a decisive role: there were more diaspores and species when wearing short-top shoes and short trousers than long ones. Even though our study was carried out mainly in natural or semi-natural habitats, a large number of dispersed species were disturbance-tolerants and weeds and only a few were specialists, suggesting the controversial role of HVD in conservation. At the individual level, people can reduce the number of diaspores through their clothing choices and diaspore removal habits, while providing adequate equipment for staff, operating cleaning stations, and increasing awareness of employees are main ways in which unintended diaspore dispersal can be tackled at the institutional level.

Exploring site-specific N application rate to reduce N footprint and increase crop production for green manure-rice rotation system in southern China

Milk vetch (Astragalus sinicus L.) is leguminous green manure (GM) which produces organic nitrogen (N) for subsequent crops and is widely planted and utilized to simultaneously reduce the use of synthetic N fertilizer and its environmental costs in rice systems. Determination of an optimal N application rate specific to the GM-rice system is challenging because of the large temporal and spatial variations in soil, climate, and field management conditions. To solve this problem, we developed a framework to explore the site-specific N application rate for the low-N footprint rice production system in southern China based on multi-site field experiments, farmer field survey, and process-based model (WHCNS_Rice, soil water heat carbon nitrogen simulator for rice). The results showed that a process-based model can explain >83.3% (p < 0.01) of the variation in rice yield, aboveground biomass, crop N uptake, and soil mineral N. Based on the scenario analysis of the tested WHCNS_Rice model, the simple regression equation was developed to implement site-specific N application rates that considered variations in GM biomass, soil, and climatic conditions. Simulation evaluation on nine provinces in southern China showed that the site-specific N application rate reduced regional synthetic N fertilizer input by 29.6 ± 17.8% and 65.3 ± 23.0% for single and early rice, respectively; decreased their total N footprints (NFs) by 23.4% and 49.3%, respectively; and without reduction in rice yield, compared with traditional farming N practices. The reduction in total NF was attributed to the reduced emissions from ammonia volatilization by 35.2%, N leaching by 28.4%, and N runoff by 32.7%. In this study, we suggested a low NF rice production system that can be obtained by combining GM with site-specific N application rate in southern China.

Yield of lucerne-grass mixtures did not differ from lucerne pure stands in a multi-site field experiment

Changing climatic conditions in north-western Europe require adaptations in management of ley grassland, requiring new grass-legume mixture designs in response to increased variability in precipitation and warmer summers. Evaluation of lucerne (Medicago sativa L.)-grass swards with the grasses cocksfoot (Dactylis glomerata L.) and tall fescue (Festuca arundinacea Schreb.), which are potentially more tolerant to summer water shortage, have yet to be evaluated under the agro-climatic conditions of north-western Europe. We tested these grasses in three-species swards and binary lucerne grass swards and compared them against pure stands and a standard lucerne-grass mixture. The study was conducted in eight environments resulting from the combination of four soil sites during the two full main production years 2020 and 2021 with an annual precipitation range of 416–839 mm. Swards were harvested three to five times annually and the accumulated herbage dry matter (DM) and nitrogen (N) yields, as well as species proportions were recorded. We found an average yield of 11.1 t DM ha-1 and no significant differences in herbage DM yields between lucerne pure stands and the different mixture swards. This pattern was stable irrespective of environmental conditions, but in general herbage yield and aboveground N yield were affected by precipitation amount. Swards with cocksfoot had a greater grass proportion than those with tall fescue. The N yield of lucerne-grass swards was lower and declined as the grass proportion increased, and reached on average 353 kg N ha-1. The maximum N yield of lucerne pure stands was 547 kg N ha-1. Species mixtures yielded higher than expected from the pure stands (net mixture effect, NE) pointing at overyielding. Consequently, synergistic interspecific interactions occurred but did not lead to transgressive overyielding. The results indicate that the inclusion of grass species with tolerance to water shortage into lucerne-based swards offers the potential to diversify grasslands without losses of agronomic potential, at least during the first two main production years.

Save More Today or Tomorrow: The Role of Urgency in Precommitment Design

To encourage farsighted behaviors, previous research suggests that marketers should invite consumers to precommit to adopting these behaviors “later.” However, the authors propose that people will draw different inferences from different types of precommitment offers, and that these inferences can help explain when precommitment is (and is not) effective at increasing adoption of farsighted behaviors. Specifically, the authors theorize that simultaneously offering consumers the opportunity to adopt a farsighted behavior now or later (i.e., offering “simultaneous precommitment”) may signal that the behavior is not urgently recommended; however, offering consumers the opportunity to adopt that behavior immediately and then, only if they decline, inviting them to adopt it later (i.e., offering “sequential precommitment”) may signal just the opposite. In a multisite field experiment (N = 5,196), the authors find that simultaneously giving consumers the chance to increase their savings now or later reduced retirement savings. Two preregistered lab studies (N = 5,080) show that simultaneous precommitment leads people to infer that taking action is not urgently recommended, and such inferences predict less adoption of recommended behaviors. Importantly, offering sequential precommitment increases inferred urgency, predicting greater adoption. Together, this research advances knowledge about the limits and potential of precommitment.

Reduction in Nitrogen Rate and Improvement of Nitrogen Use Efficiency without Loss of Peanut Yield by Regional Mean Optimal Rate of Chemical Fertilizer Based on a Multi-Site Field Experiment in the North China Plain

It is important to quantify nutrient requirements and optimize fertilization to improve peanut yield and fertilizer use efficiency. In this study, a multi-site field trial was conducted from 2020 to 2021 in the North China Plain to estimate nitrogen (N), phosphorus (P), and potassium (K) uptake and requirements of peanuts, and to evaluate the effects of fertilization recommendations from the regional mean optimal rate (RMOR) on dry matter, pod yield, nutrient uptake, and fertilizer use efficiency. Results show that compared with farmer practice fertilization (FP), optimal fertilization (OPT) based on the RMOR increased peanut dry matter by 6.6% and pod yield by 10.9%. The average uptake rates of N, P, and K were 214.3, 23.3, and 78.4 kg/ha, respectively, with 76.0% N harvest index, 59.8% P harvest index, and 41.4% K harvest index. The OPT treatment increased N, P, and K uptake by 19.3%, 7.3%, and 11.0% compared with FP, respectively. However, the average of yield, nutrition uptake, and harvest indexes of N, P, and K were not significantly affected by fertilization. The peanut required 42.0 kg N, 4.6 kg P, and 15.3 kg K to produce 1000 kg of pods. The OPT treatment significantly improved the N partial factor productivity and N uptake efficiency but decreased the K partial factor productivity and K uptake efficiency. The present study demonstrates that fertilizer recommendations from RMOR improve N use efficiency, and reduce N and P fertilizer application without yield loss in regions with smallholder farmers, and the corresponding estimation of nutrient requirements helps to make peanut fertilization recommendations.

Symbiotic nitrogen fixation does not stimulate soil phosphatase activity under temperate and tropical trees.

Symbiotic nitrogen (N)-fixing plants can enrich ecosystems with N, which can alter the cycling and demand for other nutrients. Researchers have hypothesized that fixed N could be used by plants and soil microbes to produce extracellular phosphatase enzymes, which release P from organic matter. Consistent with this speculation, the presence of N-fixing plants is often associated with high phosphatase activity, either in the soil or on root surfaces, although other studies have not found this association, and the connection between phosphatase and rates of N fixation-the mechanistic part of the argument-is tenuous. Here, we measured soil phosphatase activity under N-fixing trees and non-fixing trees transplanted and grown in tropical and temperate sites in the USA: two sites in Hawaii, and one each in New York and Oregon. This provides a rare example of phosphatase activity measured in a multi-site field experiment with rigorously quantified rates of N fixation. We found no difference in soil phosphatase activity under N-fixing vs. non-fixing trees nor across rates of N fixation, though we note that no sites were P limited and only one was N limited. Our results add to the literature showing no connection between N fixation rates and phosphatase activity.

Development of a Runoff Pollution Empirical Model and Pollution Machine Learning Models of the Paddy Field in the Taihu Lake Basin Based on the Paddy In Situ Observation Method

Agricultural non-point source (NPS) pollution has become a prominent problem for China’s water quality. Paddy fields pose a high risk of pollution to surrounding water bodies. The paddy in situ observation method (PIOM) can calculate the runoff pollution load of paddy fields in situ without changing the original runoff characteristics and agricultural water management measures. In this study, we carried out multisite field experiments during the rice growing period in the Taihu Lake basin and calculated the runoff pollution loads. Then, we developed a runoff pollution empirical model (RPEM) and runoff pollution machine learning models of paddy fields. Based on the PIOM, the average runoff volume was 342.1 mm, and the runoff pollution loads mainly occurred in the early-stage seedling and tillering stages. The mean TN, NH4+-N, TP and CODMn loads of paddy fields were 10.28, 3.35, 1.17 and 23.49 kg·ha−1, respectively. The mean N and P fertilizer loss rates were 4.09 and 1.95%, respectively. The RPEM mainly included the runoff model and surface water concentration model of paddy fields, the performance of which was validated based on the PIOM. The irrigation and runoff volumes of Zhoutie paddy (ZT) and Heqiao paddy (HQ) analyzed by RPEM and PIOM had an absolute difference of 1.2~3.1%. With the exception of the difference in CODMn loads of ZT, the absolute differences in TN, NH4+-N, TP and CODMn loads of ZT and HQ measured by two methods were less than 20%. This result illustrates the accuracy and feasibility of the RPEM for analysis of the water balance and runoff pollution loads of paddy fields. Based on 114 field runoff pollution datasets, RF provided the best machine learning model for TN, NH4+-N and TP, and SVM was the best model for CODMn. The training set R2 values of the best models for TN, NH4+-N and CODMn were above 0.8, and the testing set R2 values of the best models were above 0.7. The runoff pollution RF and SVM models can support the calculation and quantitative management of paddy field pollution load. This study provides a theoretical basis and technical support for the quantification of runoff pollution load and the formulation of pollution control measures in the Taihu Lake basin.

Continuous manure application strengthens the associations between soil microbial function and crop production: Evidence from a 7-year multisite field experiment on the Guanzhong Plain

A 7-year multisite field experiment was performed to assess the relative importance of climate, soil, and microbial properties of crop production in a wheat cropping system under contrasting fertilization management (i.e., continuous organic vs. chemical fertilization). The results showed that continuous organic application increased the annual grain yield and soil organic carbon by an average of 13.2% and 38.0%, respectively, compared to chemical fertilization. Continuous organic application increased the soil pH, which led to a significant increase in bacterial richness (i.e., Chao1) and diversity (i.e., Shannon index). Notably, organic application favored the growth of Proteobacteria and Actinobacteria but reduced the abundances of Bacteroides, Verrucomicrobia, and Acidobacteria, which ultimately led to a more complicated bacterial co-occurrence network. Importantly, organic application increased the abundances of functional genes involved in C fixation (e.g., sdhD, ACO, and acsB), C degradation (e.g., pulA, celC, and CBH2), N fixation (e.g., anfG and amoC) and nitrification (e.g., nifK), as well as P transporters (e.g., ugpQ and phnC). The structural equation modeling (SEM) results indicated that the annual air temperature and soil pH under chemical fertilizer treatment and the bacterial community structure and functional gene abundance under treatment with organic application were the primary drivers of the yield performance. Variance partitioning analysis further revealed that under chemical fertilization, climate, soil, the bacterial community, and potential function explained 32.0%, 26.7%, 19.9% and 21.4%, respectively, of the overall variance in crop productivity; however, these values shifted to 16.6%, 8.3%, 34.5% and 40.6%, respectively, when organic fertilizer was applied. These findings suggest that continuous organic application reduces the contribution of climate and edaphic conditions to crop yield compared to chemical fertilization but strengthens the associations between soil microbial function and crop production. Overall, this work provides new insights into the complexity of fertilization-induced climate-plant-microbial interactions and highlights the importance of intensified biological interactions for the sustainability of cropping ecosystems.

Pooling Interactions Into Error Terms in Multisite Experiments

Multisite field experiments using the (generalized) randomized block design that assign treatments to individuals within sites are common in education and the social sciences. Under this design, there are two possible estimands of interest and they differ based on whether sites or blocks have fixed or random effects. When the average treatment effect is assumed to be identical across sites, it is common to omit site by treatment interactions and “pool” them into the error term in classical experimental design. However, prior work has not addressed the consequences of pooling when site by treatment interactions are not zero. This study assesses the impact of pooling on inference in the presence of nonzero site by treatment interactions. We derive the small sample distributions of the test statistics for treatment effects under pooling and illustrate the impacts on rejection rates when interactions are not zero. We use the results to offer recommendations to researchers conducting studies based on the multisite design.

Peanut and cotton intercropping increases productivity and economic returns through regulating plant nutrient accumulation and soil microbial communities

BackgroundIntercropping (IC) has been widely adopted by farmers for enhancing crop productivity and economic returns; however, the underpinning mechanisms from the perspective of below-ground interspecific interactions are only partly understood especially when intercropping practices under saline soil conditions. By using permeable (100 μm) and impermeable (solid) root barriers in a multi-site field experiment, we aimed to study the impact of root-root interactions on nutrient accumulation, soil microbial communities, crop yield, and economic returns in a peanut/cotton IC system under non-saline, secondary-saline, and coastal saline soil conditions of China.ResultsThe results indicate that IC decreased the peanut pods yield by 14.00, 10.01, and 16.52% while increased the seed cotton yield by 61.99, 66.00, and 58.51%, respectively in three experimental positions, and consequently enhanced the economic returns by compared with monoculture of peanut (MP) and cotton (MC). The higher accumulations of nutrients such as nitrogen (N), phosphorus (P), and potassium (K) were also observed in IC not only in the soil but also in vegetative tissues and reproductive organs of peanut. Bacterial community structure analysis under normal growth conditions reveals that IC dramatically altered the soil bacterial abundance composition in both peanut and cotton strips of the top soil whereas the bacterial diversity was barely affected compared with MP and MC. At blossom-needling stage, the metabolic functional features of the bacterial communities such as fatty acid biosynthesis, lipoic acid metabolism, peptidoglycan biosynthesis, and biosynthesis of ansamycins were significantly enriched in MP compared with other treatments. Conversely, these metabolic functional features were dramatically depleted in MP while significantly enriched in IC at podding stage. Permeable root barrier treatments (NC-P and NC-C) counteracted the benefits of IC and the side effects were more pronounced in impermeable treatments (SC-P and SC-C).ConclusionPeanut/cotton intercropping increases crop yield as well as economic returns under non-saline, secondary-saline, and coastal saline soil conditions probably by modulating the soil bacterial abundance composition and accelerating plant nutrients accumulation.

Benefits and Limitations to Plastic Mulching and Nitrogen Fertilization on Grain Yield and Sulfur Nutrition: Multi-Site Field Trials in the Semiarid Area of China.

Plastic mulching (PM) is widely used to improve crop water use efficiency and grain yield, but few studies have reported the effects of PM on cereal crop quality, especially sulfur (S) nutrition of wheat, which has significant effects on grain protein content, dough rheology, baking quality and human health. To fill this knowledge gap, we conducted a multi-site field experiment on the Loess Plateau from 2014 to 2016 to study the effects of PM combined with nitrogen (N) fertilizer on grain yield, shoot S accumulation, and grain S concentration of winter wheat in dryland. Compared with no mulching (NM), PM increased grain yield by 13.7% but decreased grain S concentration, S requirement for 1,000 kg–1 grain, soil available S concentration, and post-anthesis S uptake by 9.0, 9.7, 24.4, and 51.8%, respectively. Plastic mulching significantly increased shoot S accumulation at anthesis by 19.2%, but there was no significant difference at maturity. Additionally, grain S concentration and S requirement had a linear-plateau relationship with N fertilization amount, reaching maximum values at 110 and 127 kg N ha–1 under PM, 37.5 and 27.0% higher than those under NM. Furthermore, shoot S accumulation and N application rates well-fitted the linear-plateau model at anthesis and maturity. At maturity, straw, grain, and shoots accumulated the most S at threshold N rates of 120, 85 and 110 kg N ha–1, respectively. Crucially, stem + leaf S concentration at anthesis had a significant linear relationship with grain S concentration under PM; a 1 g kg–1 increase in stem leaf concentration corresponded with a 0.24 g kg–1 increase in grain S concentration. This study’s findings suggest that combining soil S supplementation with optimal N fertilizer under PM in northwest China and other regions with similar cropping systems increases grain S concentration and improves nutritional and processing qualities.

Continuous Manure Application Strengthens the Associations between Soil Microbial Function and Crop Production: Evidence from a 7-Year Multisite Field Experiment in the Guanzhong Plain

Continuous Manure Application Strengthens the Associations between Soil Microbial Function and Crop Production: Evidence from a 7-Year Multisite Field Experiment in the Guanzhong Plain

A Multi-Site Evaluation of Winter Hardiness in Indigenous Alfalfa Cultivars in Northern China

Integration of perennial grass species into the current food production systems, especially in the agropastoral regions worldwide, may produce multiple benefits including, among others, a more stable productivity and a smaller eco-environmental footprint. However, one of the fundamental challenges facing the large-scale adoption of such grass species is their ability to withstand the vagaries of winter in these regions. Here, we present a comprehensive evaluation of the winter hardiness of 50 indigenous Chinese cultivars of alfalfa, a high-quality leguminous perennial grass, in comparison with six introduced U.S. cultivars in a multi-site field experiment in northern China. Our results reveal that indigenous cultivars have stronger winter hardiness than introduced cultivars. Cultivars native in the north performed better than southern cultivars, suggesting that suitability evaluation is an unavoidable step proceeding any regional implementations. Our results also show that the metric we used to assess alfalfa’s winter hardiness, the average score index (ASI), produced more consistent results than another more-widely used metric of winter survival rate (WSR). These findings offer a systematic field evidence that supports regional cropping system adjustment and production system betterment to ensure food security under climate change in the region and beyond.

Decomposition in Mixed Beech Forests in the South-Western Alps Under Severe Summer Drought

Climate and plant litter diversity are major determinants of carbon (C) and nitrogen (N) cycling rates during decomposition. Yet, how these processes will be modified with combined changes in climate and biodiversity is poorly understood. With a multisite field experiment, we studied the interactive effects of reinforced and prolonged summer drought (using rainout shelters) and tree species mixing on leaf litter decomposition in beech forests in the French Alps. Forests included monospecific stands of Fagus sylvatica, Abies alba and Quercus pubescens and two-species mixtures composed of beech and one of the other species. We hypothesized (1) slower C and N release during decomposition in response to experimentally prolonged summer drought, but (2) less so in mixed compared to monospecific tree stands, due to indirect canopy effects and direct litter mixing effects. Litter lost 35% of initial C and 15% of N on average across all sites and litter types over 30 months of decomposition. The experimentally prolonged summer drought led to weakly slower C loss but had no effect on N loss. Tree species mixing did not alter drought effects on decomposition but had non-additive effects on C and N loss, which were dominated by direct litter mixing rather than indirect plot-specific tree species composition effects. Our data suggest relatively small effects of reinforced and prolonged summer drought on decomposition, possibly because process rates are generally slow during summer and because microsite variability is larger than the effects of rainfall exclusion. The dominant contribution of litter mixing to the overall effect of plot-specific tree species mixtures on decomposition supports the importance of microsite conditions for C and N dynamics during decomposition, which should be accounted for more explicitly in climate and biodiversity change predictions.

The main drivers of methane emissions differ in the growing and flooded fallow seasons in Mediterranean rice fields

PurposeTo assess 1) the cumulative greenhouse gas emissions –GHG- and global warming potential (methane – CH4- and nitrous oxide) from rice fields in the growing and fallow seasons, and 2) the environmental and agronomic drivers of CH4 emissions, and their relative capacity to explain CH4 variation.MethodsA two-year multisite field experiment covering the agronomic and environmental variability of a rice growing area in NE Iberian Peninsula was conducted with monthly samplings of GHG and monitoring of both environmental and agronomic factors. Information-theoretic framework analysis was used to assess the relative contribution of the environmental and agronomic variables on methane emissions.ResultsTwo thirds of the CH4 is emitted in the fallow season. Edaphic factors exert more influence during the growing season whereas agronomic factors have a higher impact in the fallow. The implications of these findings on the design of improved mitigation options rice are discussed.ConclusionsSoils with higher soil sulphate concentration, bulk density and clay content emit less CH4 in growing season. In the fallow season, the rates of both straw input and nitrogen fertilization stimulate CH4 emissions.Graphical abstract