In this paper, the RF energy harvesting system and RF-based wireless power transfer system are proposed and designed for battery-less self-sustaining application. For energy harvesting, the designed antenna array improves the received RF power effectively and also can harvest RF energy in multi-frequency bands. For wireless power transfer, the proposed helical antenna realizes the system design in miniaturization. Subsequently, the T shape LC matching network are designed between the antenna and the rectifying circuit to obtain more power transmission. The measured results show that the proposed Wi-Fi rectifier and 433 MHz rectifier offer a maximum conversion efficiency of 66.8% and 76% in case of the input power is −3 dBm and 0 dBm, respectively. Finally, the performance of the RF-based wireless power transfer system and RF energy harvesting system are attested by experimentally measurement, the measured results indicate that these systems can be used to power electronic.