Multifaceted Investigation Research Articles

Chlorpromazine's impact on Mytilus galloprovincialis: a multi-faceted investigation

The antipsychotic chlorpromazine (Cpz) has raised concern as a pharmaceutical effluent due to its wide medical applications. Moreover, its potent pro-oxidant properties and impact on the cell viability of the marine mollusc Mytilus galloprovincialis, even at low concentrations (ng/L), have been noted. Based on this evidence, in this study, we investigated the physiological effects of Cpz on M. galloprovincialis, to elucidate its fate within the organism, in terms of bioaccumulation, biotransformation, byssus changes and stress responses of the cellular thiolome. Histological and indicators of vitality analyses were also performed to better evaluate the influence of the drug on the morphology and cell viability of the digestive gland. To this end, two different concentrations of Cpz (Cpz I (12 ng/L or 37 pM) and Cpz II (12 μg/L or 37 nM)) were administered to mussels over 14 days. Cpz accumulation in the digestive gland significantly increased with water concentration (BCF of Cpz I and Cpz II). Biochemical analyses indicated lysosomal dysfunction, reflected in elevated total Cathepsin D activity and compromised lysosomal membrane stability. Stress-related and metal-buffering proteins (GST and metallothionein) responded to both Cpz concentrations. Cpz I induced phase I biotransformation activity (CYP450-dependent EROD), while Cpz II triggered caspase-3 activation, indicative of detoxification overload. Histological analysis revealed digestive gland atrophy, epithelial thinning, haemocyte infiltration, and brown cell presence. Byssus analysis showed significant alterations. In conclusion, our study underscores Cpz-induced physiological and histological changes in M. galloprovincialis, posing potential implications for mussel health and confirming the utilisation of this mussel as an indication of Cpz ecotoxicity.

Repurposing FDA-Approved Anastrozole-based Drugs for Breast Cancer through Drug-Drug Transcriptomic Similarity and Cavity Detection Guided Blind Docking

Breast cancer remains a significant global health concern, necessitating innovative strategies for drug discovery and development. Repurposing existing drugs offers a promising avenue, leveraging the wealth of information available on food and drug administration (FDA) approved compounds. This study investigates the repurposing potential of anastrozole-based drugs, already established for their efficacy in certain conditions, for breast cancer treatment. This research presents a multifaceted investigation into the identification, structural refinement, and virtual screening of potential therapeutics targeting the aromatase CYP19A enzyme, a crucial player in hormone-related conditions such as breast cancer. The study commences with the identification of anastrozole-based drugs exhibiting transcriptomic profiles closely resembling known breast cancer therapeutics. Through a comprehensive analysis, a subset of compounds demonstrates high transcriptomic similarity, suggesting shared molecular pathways and target interactions. Notably, anastrozole, a well-known aromatase inhibitor, emerges as a top candidate, highlighting its potential in breast cancer treatment. The crystallographic structure of aromatase CYP19A is subjected to meticulous preprocessing using the PDB-REDO server, resulting in significant improvements in various validation metrics. Structural changes, including alterations in rotamers, removal of water molecules, and peptide flips, indicate the success of the refinement process in enhancing the accuracy of the protein model. The refined structure serves as a reliable foundation for subsequent studies. Further, structure-based cavity detection unveils potential binding sites on the aromatase enzyme. Docking studies employing the cb-dock server elucidate the interaction patterns and binding affinities of selected compounds within these cavities. Anastrozole, along with other candidates like dolasetron and stiripentol, exhibits promising binding scores and interacts with specific residues crucial for enzyme activity. This integrative approach, combining transcriptomic similarity analysis, structural refinement, and virtual screening, provides valuable insights into potential lead compounds for the inhibition of aromatase in breast cancer therapy. The identified compounds offer a starting point for further experimental validation and drug development. The most promising compound that can be repurposed for as an aromatase inhibitor is dolasetron. Overall, this research contributes to the ongoing efforts to leverage computational methodologies for the rational design of targeted therapeutics against hormone-related disorders.

Impact assessment of long-term high-volume abstraction of groundwater from Palla floodplain aquifers of Delhi, India

The global demand for freshwater resources has universally stressed the groundwater system. This is particularly seen in urban areas like the National Capital Territory (NCT), Delhi where extreme disturbance in the natural flow conditions may result in a variety of complex hydrological crises. To address the issues of uneven water distribution in NCT-Delhi, a detailed assessment was undertaken to formulate a holistic approach to efficiently utilize natural water in the late 1990s and early 2000s, leading to the development of the Palla well field, which has been deemed successful on the water supply front. However, its hydrogeological impact assessment was not fully reviewed until now.In this context, the study presented here aims to assess the impact of the long-term pumping from Palla well field tube wells on the physical and chemical attributes of the hydrogeological system. The experimental investigations revealed that the aquifers have high potential, and can achieve dynamic equilibrium relatively faster, thereby showing resilience to the drastic effects of prolonged pumping. Furthermore, the isotopic signatures revealed varying impacts of recharge associated with given hydrological settings.Additionally, the XRD and SEM-EDS analyses indicate that the sediment-groundwater interaction does not primarily control the major ion chemistry in the younger alluvium. However, iron enrichment in a few groundwater samples was supposedly observed to be a product of the chemical weathering of iron oxides. Overall, the multifaceted investigation provided evidence that there is no major detrimental impact on the groundwater system from the prolonged pumping in the Palla wellfield.

Insights into the structural, electronic, quantum chemical properties and molecular docking studies on novel NAMPT inhibitor molecule

This research paper presents a comprehensive study on a novel NAMPT (Nicotinamide phosphoribosyl transferase) inhibitor molecule, N- [4- [(4R)-1,4-dimethyl-6-oxidanylidene-4,5-dihydropyridazin-3-yl] phenyl]-5,7-dihydropyrrolo [3,4-b] pyridine-6-carboxamide], with the potential applications in cancer therapy. We performed quantum chemical calculations with DFT and topological investigations on the molecule to understand its electronic properties and chemical behaviour. The study covers basic molecular properties, molecular planarity and various electronic properties, including the HOMO (Highest Occupied Molecular Orbital), LUMO (Lowest Unoccupied Molecular Orbital) energy levels, the energy gap of 4.09 eV, ionization potential, electron affinity, electronegativity, chemical potential, chemical hardness, chemical softness, electrophilicity index, Mulliken atomic charges, Laplacian bond order, and ESP (Electrostatic Potential) properties. Moreover, we studied the nature of bonding and interactions within the molecule using ELF (Electron Localization Function), LOL (Localized Orbital Locator), RDG (Reduced Density Gradient), and NCI (Non-covalent Interaction). An analysis of the title molecule's molecular thermodynamic properties demonstrates its intrinsic stability under thermal and quantum effects. Molecular docking studies were also performed to reveal how the molecule interacts with its target protein, exhibiting a strong binding affinity for NAMPT target proteins (7PPF and 5WI0), which could help develop future cancer drugs. This multifaceted investigation provides a detailed understanding of the molecular structure and interactions of the molecule with NAMPT protein, potentially paving the way for its potential use as a cancer therapy.

A multifaceted Investigation Saga of an Sordid Act of Mariticide - A Case Report

A multifaceted Investigation Saga of an Sordid Act of Mariticide - A Case Report

Multifaceted investigation of occupational wellbeing profiles among guidance counsellors

ABSTRACT Educational guidance counsellors’ wellbeing at work was studied based on the circumplex model of occupational wellbeing. The main aim was to identify what kind of occupational wellbeing profiles (OWP) representing different levels and combinations of burnout, work engagement, workaholism and job satisfaction existed in this group. Data were collected via e-surveys in two separate samples: Sample 1 in 2019 (n = 211) and Sample 2 baseline in 2020 (n = 343) and follow-up in 2021 (n = 176). Latent profile analysis revealed altogether three OWPs: Satisfied-Engaged (68% in 2019, 53% in 2020, 56% in 2021), Workaholic-Engaged (28%, 42%, 33%) and Burned-out (4%, 5%, 11%). In addition, results concerning Sample 2 with a longitudinal design showed a decline in occupational well-being during the COVID-19.

Economic appraisal and strategic analysis of the onion industry in the Philippines

This research undertakes a comprehensive evaluation of the economic dynamics within the onion industry, concentrating specifically on the prolific "Onion Basket of the Philippines" and its extension throughout Southeast Asia. Employing the value chain model as a methodological scaffold, the study meticulously dissects the intricate fabric of activities within this sector, aiming to pinpoint the exact operations that confer amplified value and competitive edge to this agricultural commodity. Through a meticulous selection process encompassing 360 agrarian participants, primarily sourced from the preeminent onion-producing municipalities of Nueva Ecija—namely, Bongabon, Gabaldon, and Laur—this investigation orchestrates an exhaustive scrutiny of the financial implications and returns. By comparing analyses across both traditional post-harvest marketing and the integration of cold storage infrastructure, the study unveils pronounced differentials. Evidently, the incorporation of cold storage resources precipitates substantial revenue escalation for farmers, in stark contrast to immediate post-harvest sales. However, the accompanying capital outlay affiliated with cold storage mandates judicious deliberation. Given the considerable financial connotations, this research underscores the imperativeness of establishing rigorous regulatory frameworks governing onion cold storage practices. Furthermore, acknowledging the intricate mesh of interests embedded within the onion value chain, it advocates heightened vigilance and stringent oversight concerning stakeholders. This multifaceted investigation, beyond contributing empirical authenticity, serves as a clarion call for prudent stewardship of this pivotal agricultural domain.

Moringa oleifera Lam. Commercial Beverages: A Multifaceted Investigation of Consumer Perceptions, Sensory Analysis, and Bioactive Properties.

This study employs a multidisciplinary approach to evaluate consumers' perceptions and acceptance of Moringa oleifera Lam. beverages, examining sensory attributes, chemical composition, and bioactivities. High-performance liquid chromatography with diode array detection (HPLC-DAD) analyses revealed significant chemovariation in phenolic compositions among commercial moringa beverages. A soluble moringa powder drink exhibited the greatest concentrations of phenolic and flavonoid compounds, along with powerful antioxidant capacity powers assessed with ABTS•+, DPPH•, FRAP assays, •NO, and H2O2 scavenging activities. However, this sample was the least preferred and presented high Cd levels, exceeding WHO-acceptable values of 0.3 mg/kg. Sensory testing indicated that sweet and floral flavors contributed to beverages being liked, while green, grass, herbal flavors, sour, bitter, and precipitate presence were considered unfavorable sensory attributes. Health claims positively influenced acceptance, particularly among women. Consumers associated feelings of health, wellness, relaxation, and leisure with moringa beverages. During purchase, the most observed information included the ingredient list, health benefits, and type/flavor. These findings emphasize the importance of consumer awareness in reading labels, verifying product origins, and ensuring the absence of contaminants. By understanding consumer preferences and the impact of health claims, producers can better tailor M. oleifera beverages to meet consumer expectations while maintaining safety and quality standards.

Temperature and field dependences of magnetic fluid's shear viscosity: Decoupling inputs from a carrier fluid and magnetic nanoparticles

In this work, we report a multifaceted investigation of the magnetoviscous effect for a magnetic fluid cylinder locked in a tube by an inhomogeneous magnetic field and exhibiting damped oscillations. It is shown that the acquisition of viscosity elevation should take into account not only the magnetic field strength but also the system's temperature and the rate of oscillations due to their significant influence on the carrier's viscosity including non-Newtonian properties of the colloidal system of magnetic nanoparticles even in absence of the external magnetic field. The part of the viscosity change that originated from the interaction with the magnetic field is determined explicitly. The perspectives of the oscillatory method for determining the characteristics of ferrofluids are discussed.

Does financial development enhance the growth effect of FDI? A multidimensional analysis in emerging and developing Asia

PurposeThis study examines the role of financial development (FD) in enhancing the growth effect of foreign direct investment (FDI) in emerging and developing Asia from 1996 to 2019.Design/methodology/approachThe study exploits the new broad-based Financial Development Index of the International Monetary Fund (IMF) and adopts panel smooth transition regression (PSTR) to perform alternative empirical models for a multidimensional analysis of the FD threshold effect in the growth–FDI nexus.FindingsThe results show two thresholds of FD mediating the nonlinear effect of FDI on growth. FD beyond a certain level will enhance the growth effect of FDI, but very high levels of FD will not induce foreign investment to benefit economic growth in emerging and developing Asian economies. The impact of financial institutions on the FDI–growth link is stronger than that of financial markets. Besides, FDI’s effect on growth has an inverted-U shape conditional on financial depth, whereas it is positively associated with the accessibility and efficiency of the financial system.Practical implicationsThese results suggest policy implications for emerging and developing Asian countries, emphasizing the other side of “too much finance” and the potential for improvement in the access to and efficiency of the financial system to boost the effects of FDI and FD in the growth of these economies.Originality/valueThe study is the first multifaceted investigation into the influence of FD on the growth effect of FDI. Beyond the previous empirical evidence showing only the impact of credit from banking sector, this study shows different mediating effects of different financial sectors and three dimensions of financing (depth, access and efficiency). The study suggests essential implications for the region in adjusting long-run policies to enhance the FDI–FD–growth link.

A multifaceted approach to understanding unexpected sound change

Abstract This paper demonstrates that unexpected sound changes are best explained by an approach that accounts for different motivations: phonetic, structural and social. Here, we focus on a multifaceted investigation of the cross-linguistically uncommon bilabial trills to show the complex interaction between different drivers of sound change. In this paper, we highlight and examine the prenasalized voiced bilabial trill mʙ and plain voiceless bilabial trill p [ʙ̥] found in a number of Oceanic languages spoken on Malekula Island in Vanuatu. We offer a comparative-historical analysis, and we identify the various forces that have led to the emergence and persistence of mʙ and p in Malekula languages: the historical articulatory environments, the particular make-up of the consonant inventories of these languages, complementary sound changes and phonological processes, contact with non-Austronesian languages, and in-group identity attachment. Furthermore, we offer a hypothesis for the relative timing of these factors on the historical pathway of Malekula’s bilabial trills.

Exploring the Five Influential Action Factors to Unravel Procrastination

The voluntary delaying of work completion in the face of potential drawbacks is known as procrastination. It has substantial effects on personal, academic, and professional spheres. In order to better comprehend procrastination's underlying mechanisms, contributory elements, and potential mitigation measures, this paper aims to present a thorough and multifaceted investigation of the procrastinatory behaviour. This paper highlights a detailed examination of the cognitive, affective, and motivational elements of procrastination by synthesising the available literature from the psychology and cognitive science domains. There are five potential reasons as to why people procrastinate: when goals are too big, when goals are too small, and issues with respect to belief, attitude, as well as skill deficits. The paper also highlights a mitigation model basing analysis of chunking and Neuro-Linguistic Programming to end the vicious cycle with respect to procrastinatory behaviour.

La vida en la frontera: protocol for a prospective study exploring stress and health resiliencies among Mexican-origin individuals living in a US-Mexico border community

BackgroundMexican-origin adults living near the U.S.-Mexico border experience unique and pervasive social and ecological stressors, including poverty, perceived discrimination, and environmental hazards, potentially contributing to the high burden of chronic disease. However, there is also evidence that residents in high-density Mexican-origin neighborhoods exhibit lower prevalence rates of disease and related mortality than those living in other areas. Understanding the factors that contribute to health resiliencies at the community scale is essential to informing the effective design of health promotion strategies.MethodsLa Vida en la Frontera is a mixed-methods participatory study linking a multi-disciplinary University of Arizona research team with Campesinos Sin Fronteras, a community-based organization founded by community health workers in San Luis, Arizona. This paper describes the current protocol for aims 2 and 3 of this multi-faceted investigation. In aim 2 a cohort of N≈300 will be recruited using door-to-door sampling of neighborhoods in San Luis and Somerton, AZ. Participants will be surveyed and undergo biomarker assessments for indicators of health and chronic stress at three time points across a year length. A subset of this cohort will be invited to participate in aim 3 where they will be interviewed to further understand mechanisms of resilience and wellbeing.DiscussionThis study examines objective and subjective mechanisms of the relationship between stress and health in an ecologically diverse rural community over an extended timeframe and illuminates health disparities affecting residents of this medically underserved community. Findings from this investigation directly impact the participants and community through deepening our understanding of the linkages between individual and community level stress and chronic disease risk. This innovative study utilizes a comprehensive methodology to investigate pathways of stress and chronic disease risk present at individual and community levels. We address multiple public health issues including chronic disease and mental illness risk, health related disparities among Mexican-origin people, and health protective mechanisms and behaviors.

Surface modification of 316L stainless steel with multifunctional locust gum/polyethylene glycol-silver nanoparticles using different coating methods

316 L stainless steel (SS316L) has attracted significant attention in both implantable and non-implantable medical devices for its biocompatibility. However, bacterial adhesion on the surface of SS316L and corrosion after contact with body fluids challenge its usability as a bioimplant. It is therefore necessary to improve the antibacterial and anticorrosion properties of SS316L. One of the most effective ways to achieve this is to deposit a protective metal nanoparticles-based coating on the surface of SS316L. In this study, organic Locust Gum/polyethylene glycol‑silver nanoparticles (Loc/PEG-Ag NPs) were synthesized with sonochemical method, and characterized to determine their morphology, crystal structure, chemical functional groups, and antioxidant capacity. The synthesized Ag NPs were coated on SS316L with drop casting and airbrush spray coating methods. The morphological characteristics, superficial properties, antibacterial activity, and corrosion resistivity of the surface modified SS316L were evaluated. A multifaceted investigation was performed to understand the surface characteristics and to improve the productivity of the Ag NPs coating on the metallic surface. The antioxidant capacities of the spherical-shaped Loc/PEG-Ag NPs with an average size of 10 to 50 nm were found to be 17.90 ± 0.50 and 20.47 ± 0.19 mmol Trolox equivalent (TE)g−1, respectively. The airbrush spray coating treatment significantly improved the surface properties of the substrate due to the formation of a thin layer (8.47 μm), affecting interactions between Ag NPs and substrates. The Ag NPs coated SS316L demonstrated high antibacterial activity against E. coli and S. aureus at a diameter of 28 mm and 19 mm, respectively. Compared with the bare substrate, the corrosion resistance of the surface modified SS316L is improved. The results illustrated the importance of surface nano-modification in attaining favorable antibacterial and anticorrosive performance. It is believed that the Loc/PEG-Ag NPs coated SS316L could be a promising multifunctional (i.e., uniform coating +biological activity +corrosion resistance) nanoplatforms in biomedical applications.

Prefacing unexplored archives from Central Andean surface-to-bedrock ice cores through a multifaceted investigation of regional firn and ice core glaciochemistry

AbstractShallow firn cores, in addition to a near-basal ice core, were recovered in 2018 from the Quelccaya ice cap (5470 m a.s.l) in the Cordillera Vilcanota, Peru, and in 2017 from the Nevado Illimani glacier (6350 m a.s.l) in the Cordillera Real, Bolivia. The two sites are ~450 km apart. Despite meltwater percolation resulting from warming, particle-based trace element records (e.g. Fe, Mg, K) in the Quelccaya and Illimani shallow cores retain well-preserved signals. The firn core chronologies, established independently by annual layer counting, show a convincing overlap indicating the two records contain comparable signals and therefore capture similar regional scale climatology. Trace element records at a ~1–4 cm resolution provide past records of anthropogenic emissions, dust sources, volcanic emissions, evaporite salts and marine-sourced air masses. Using novel ultra-high-resolution (120 μm) laser technology, we identify annual layer thicknesses ranging from 0.3 to 0.8 cm in a section of 2000-year-old radiocarbon-dated near-basal ice which compared to the previous annual layer estimates suggests that Quelccaya ice cores drilled to bedrock may be older than previously suggested by depth-age models. With the information collected from this study in combination with past studies, we emphasize the importance of collecting new surface-to-bedrock ice cores from at least the Quelccaya ice cap, in particular, due to its projected disappearance as soon as the 2050s.

Multifaceted investigation underlies diverse mechanisms contributing to the downregulation of Hedgehog pathway-associated genes INTU and IFT88 in lung adenocarcinoma and uterine corpus endometrial carcinoma.

Hedgehog (Hh) signaling primarily functions in the control of mammalian embryonic development but also has roles in cancer. The Hh activation depends on ciliogenesis, a cellular process that describes outgrowth of the primary cilium from cell membrane. Ciliogenesis initiation requires a set of proteins known as planar cell polarity (PCP) effectors. Inturned (INTU) is a PCP effector that reportedly functions synergistically with Hh signaling in basal cell carcinoma, suggesting that INTU has an oncogenic role. In this study, we carried out a pan-cancer investigation on the prognostic significance of INTU in different types of cancer. We demonstrated that INTU downregulation correlated with reduced survival probabilities in lung adenocarcinoma (LUAD) and uterine corpus endometrial carcinoma (UCEC) patients. Similar expression patterns and prognostic values were identified for intraflagellar transport 88 (IFT88), another Hh pathway-associated gene. We elucidated multiple mechanisms at transcriptional, post-transcriptional and translational levels that involved transcription factor 4 and non-coding RNAs-associated regulatory networks contributing to the reduction of INTU and IFT88 levels in LUAD and UCEC samples. Taken together, this study demonstrates the prognostic significance of the Hh-related genes INTU and IFT88 in LUAD and UCEC and further delineates multifaceted mechanisms leading to INTU and IFT88 downregulation in tumor samples.

Clinical significance of osmophobia and its effect on quality of life in people with migraine.

In this study, we aimed to evaluate the clinical significance of osmophobia and its effect on quality of life in people with migraine. A total of 145 people with migraine were included in this cross-sectional study. Patients were evaluated with the migraine data form, the Migraine 24-Hour Quality of Life Questionnaire (24-HrMQoLQ), the Migraine Disability Assessment Scale (MIDAS), the Patient Health Questionnaire-9 (PHQ-9), the Insomnia Severity Index (ISI), the Generalized Anxiety Disorder-7 (GAD-7), the Allodynia Symptom Checklist (ASC), and the Fatigue Severity Scale (FSS). To evaluate the presence of osmophobia retrospectively, a semi-structured interview was conducted with the patients by the neurologist. The mean 24-Hr-MQoLQ of patients with osmophobia was significantly lower than those without osmophobia. The decrease in the 24-Hr-MQoLQ was statistically significant in the areas of feeling and concerns and social functionality. The mean of the MIDAS scale was higher significantly in patients with osmophobia than those without osmophobia. In addition, the mean ISI, PHQ-9, FSS and ASC scores of patients with osmophobia were statistically significantly higher than those without osmophobia. Both 24-h and 3-month quality of life of people with migraine with osmophobia were more affected than those without osmophobia. At the same manner, insomnia, depression, fatigue and allodynia were observed at higher rates in people with migraine with osmophobia than in migraine without osmophobia. Osmophobia, which is one of the specific symptoms that distinguishes migraine from other headache disorders, deserves further and multifaceted investigation.

A human single-neuron dataset for face perception

The human amygdala and hippocampus have long been associated with face perception. Here, we present a dataset of single-neuron activity in the human amygdala and hippocampus during face perception. We recorded 2082 neurons from the human amygdala and hippocampus when neurosurgical patients with intractable epilepsy performed a one-back task using natural face stimuli, which mimics natural face perception. Specifically, our data include (1) single-neuron activity from the amygdala (996 neurons) and hippocampus (1086 neurons), (2) eye movements (gaze position and pupil), (3) psychological assessment of the patients, and (4) social trait judgment ratings from a subset of patients and a large sample of participants from the general population. Together, our comprehensive dataset with a large population of neurons can facilitate multifaceted investigation of face perception with the highest spatial and temporal resolution currently available in humans.

A multi-faceted approach to determine the provenance of Eocene converging deltas, Dongying Depression, Bohai Bay Basin (E China)

The middle interval of the 3rd member of the Shahejie Formation (Es3m) of the Dongying Depression accumulated as part of a system of converging deltas derived from multiple source regions. These complex deposits that are targets of oil/gas exploration and development in eastern China have yet to be studied at the level of detail necessary to elucidate their origin and sedimentary evolution. This paper relies upon a multi-faceted investigation of the Es3m depositional system that includes analysis of clastic compositions, heavy mineral assemblages and mineral ratios, and clay mineralogy to illuminate the provenance of Es3m deposits. Compositional data is obtained by analysis of logs and core material. Our results reveal that Es3m deposits were derived from source regions to northeast (Qingtuozi Uplift and Chenjiazhuang Uplift) and southeast (Weibei Uplift and Guangrao Uplift) of the Dongying Depression. Each source region shed sediment to the study area that accumulated in distributary channels, interdistributary swamps, natural levees, mouth bars, underwater distributary channels, sand sheets and underwater distributary interchannel regions. Moreover, lacustrine shore and semi-deep lacustrine deposits that include turbidites are recognized in the studied succession. The separate deltas appear to have converged approximately halfway through the depositional history of the Es3m Formation. Convergence continued in association with delta progradation, forming overlapping sand bodies that are targets of hydrocarbon exploration.

Lysobacter ciconiae sp. nov., and Lysobacter avium sp. nov., isolated from the faeces of an Oriental stork.

Two Gram-stain-negative, mesophilic, strictly aerobic, nonspore forming, and yellow-pigmented strains with rod-shaped cells, designated H21R20T and H23M41T, were isolated from the faeces of an Oriental stork (Ciconia boyciana). Based on 16S rRNA gene sequences, both strains showed the highest similarity (98.3-98.4%) to the type strain of Lysobacter concretionis. Phylogenetic analysis based on the 16S rRNA genes and 92 bacterial core genes showed that strains H21R20T and H23M41T were robustly clustered with L. concretionis Ko07T. Whole genome sequencing revealed that the genomes of both strains were approximately 2.9 Mb in size. The DNA G + C contents of the H21R20T and H23M41T strains were 67.3 and 66.6%, respectively. The two strains showed 80.1-81.7% average nucleotide identity with L. concretionis Ko07T. Strain H21R20T grew optimally at 30°C and pH 8.0 and in the presence of 0.5-3% (wt/vol) NaCl, while strain H23M41T grew optimally at 30°C and pH 7.0-8.0 and in the presence of 0-3% (wt/vol) NaCl. Both strains possessed iso-C15:0, iso-C16:0 and summed feature 9 (iso-C17:1ω9c and/or C16:010-methyl) as the major cellular fatty acids, ubiquinone Q-8 as a predominant quinone, and diphosphatidylglycerol, phosphatidylglycerol, and phosphatidylethanolamine as the major polar lipids. A multifaceted investigation demonstrated that strains H21R20T and H23M41T represent novel species of the genus Lysobacter, for which we propose the names Lysobacter ciconiae sp. nov. and Lysobacter avium sp. nov. for strains H21R20T (= KCTC 82316T = JCM 34832T) and H23M41T (= KCTC 62676T = JCM 33223T), respectively.