This paper investigates the dynamic characteristics of a multi-degree-of-freedom (multi-DOF) acoustic resonant system involving highly viscous fluids. First, a fluid-structure interaction (FSI) model is established to describe the interaction between the multi-DOF acoustic resonant system and the two-phase fluids, and then the effects of various excitation parameters on the dynamic response of the coupled system are studied. Under the influence of vertical acoustic vibration, the fluid in the container undergoes violent and irregular motions, which leads to a decrease and fluctuation in the equivalent mass of the fluid. The reduction in fluid equivalent mass causes the excitation frequency to deviate from the natural frequency of the multi-DOF acoustic resonant system, thus significantly reducing the dynamic response of the coupled system. Furthermore, the fluctuation of the fluid equivalent mass induces a quasi-periodic motion pattern of the acoustic resonant system. Although increasing the excitation amplitude can effectively increase the dynamic response of the coupled system, it can also increase the fluctuation level of the dynamic response to a certain extent. By appropriately increasing the excitation frequency, the coupled system can operate at a new resonant frequency, thereby reducing the influence of fluid motion on the dynamic response of the system.