ObjectiveTo evaluate the impact of a multi-component school-based physical activity intervention (Fit-4-Fun) on health-related fitness and objectively measured physical activity in primary school children. MethodsFour Hunter primary schools were recruited in April, 2011 and randomized by school into treatment or control conditions. Participants included 213 children (mean age=10.72years±0.6; 52.2% female) with the treatment group (n=118) completing the 8-week Fit-4-Fun Program. Participants were assessed at baseline and 6-month follow-up, with a 91% retention rate. Cardio-respiratory fitness (CRF) (20m shuttle run) was the primary outcome, and secondary outcomes included body composition (BMI, BMIZ), muscular fitness (7-stage sit-up test, push-up test, basketball throw test, Standing Jump), flexibility (sit and reach) and physical activity (7days pedometry). ResultsAfter 6-months, significant treatment effects were found for CRF (adjusted mean difference, 1.14 levels, p<0.001), body composition (BMI mean, −0.96kg/m2, p<0.001 and BMI z-score mean −0.47 z-scores, p<0.001), flexibility (sit and reach mean, 1.52cm, p=0.0013), muscular fitness (sit-ups) (mean 0.62 stages, p=0.003) and physical activity (mean, 3253 steps/day, p<0.001). There were no group by time effects for the other muscular fitness measures. ConclusionsA primary school-based intervention focusing on fitness education significantly improved health-related fitness and physical activity levels in children.