In this work a microelectrode array was used to develop a multianalyte sensor. Glucose, L-lactate and uric acid were chosen as model analytes. The array consists of ten groups of 40 microelectrodes connected in parallel, each of a size of 36 × 36 μm. Thus the array showed a large overall current response retaining typical microelectrode features. Single rows of electrodes were modified with enzymes entrapped in a polymer matrix. As the three chosen oxidases were used as enzymes hydrogen peroxide generated by the enzymatic reaction was detected by anodic oxidation at + 800 mV vs. Ag/AgCl/3 M KCl. The elimination of interfering substances was accomplished by the application of a NAFION membrane between the electrode surface and the enzyme layer.