Mulberry Plants Research Articles

Recent advances on bioactive polysaccharides from mulberry.

Mulberry (Moraceae family), commonly considered as a folk remedy, has a long history of usage in many regions of the world. Polysaccharides regarded as one of the major components in mulberry plants, and they possess antioxidant, antidiabetic, hepatoprotective, prebiotic, immunomodulatory and antitumor properties, among others. In recent decades, mulberry polysaccharides have been widely studied for their multiple health benefits and potential economic value. However, there are few reviews providing updated information on polysaccharides from mulberry. In this review, recent advances in the study of isolation, purification, structural characterization, biological activity and the structure-activity relationship of mulberry polysaccharides are summarized and discussed. Furthermore, a thorough analysis of the current trends and perspectives on mulberry polysaccharides is also proposed. Hopefully, these findings can provide a useful reference value for the development and application of natural polysaccharides in the field of functional food and medicine in the future.

Ukrainian and introduced mullberry (Morus L.) fruit varieties

Aim. To compare the best mulberry collection accessions by resistance to diseases (cylindrosporium leaf spot, bacteriosis and wilt) and infructescence yield as well as adaptation to climatic conditions in Northeastern Ukraine. Results and Discussion. The collection of the mulberry gene pool of the Laboratory of Sericulture and Technical Entomology of the National Research Center "Institute of Experimental and Clinical Veterinary Medicine" of NAAS, which comprises 104 accessions, was studied. The article presents the results of studying features of the most promising fruit varieties of mulberry bred in Ukraine and introduced from different countries. The study showed that the Ukrainian mulberry fruit varieties from the gene pool collection were resistant to cylindrosporium leaf spot, bacteriosis and wilt. The introduced fruit varieties were weakly affected by cylindrosporium (8.0%) and bacteria (3.7%); varieties Bulgaria 24 and Kimsan were resistant to bacteriosis. Varieties Ukrainskaya 5, Ukrainskaya 6, Ukrainskaya 7, Ukrainskaya 107, Nadezhda, Kharkovskaya 14, Plodovaya 1, Yuzhnaya 6, Kimsan, Jasan, Kokuso 21, Kokuso 25, and Shimo maru were wilt-resistant. Mulberry plants of these varieties are adapted to climatic conditions of Northeastern Ukraine. The study showed that varieties Ukrainskaya 5, Ukrainskaya 6, Ukrainskaya 7, Nadezhda, Kharkovskaya 14, Kimsan, Jasan, Kokuso 21, and Shimo maru were valuable as donors of resistance to cylindrosporium leaf spot, bacteriosis and wilt. Varieties Ukrainskaya 6, Ukrainskaya 7, Ukrainskaya 107, Nadezhda, Kharkovskaya 14, Plodovaya 1, Plodovaya 3, Yuzhnaya 6, Podkumskaya, Ussuriyskaya 24, Bulgaria 59, Kimsan, Jasan, Kokuso 25, and Shimo maru consistently gave high yields of infructescences with high-quality fruits. Over the last 5 years, surveys of these mulberry varieties demonstrated that winterkill of shoots in the experimental location did not exceed 5.0%. Conclusions. Analysis of the results of studying the fruit varieties of Ukrainian and introduced mulberry from the collection of the Sericulture Laboratory showed that varieties Ukrainskaya 5, Ukrainskaya 6, Ukrainskaya 7, Nadezhda, Kharkovskaya 14, Kimsan, Jasan, Kokuso 2, and Shimo maru could be used as donors of resistance to cylindrosporium leaf spot, bacteriosis and wilt. Varieties Ukrainskaya 6, Ukrainskaya 7, Ukrainskaya 107, Nadezhda, Kharkovskaya 14, Plodovaya 1, Plodovaya 3, Yuzhnaya 6, Podkumskaya, Ussuriyskaya 24, Bulgaria 59, Kimsan, Jasan, and Shimo maru were highly adapted to the climate of Ukraine and gave stable and high yields of infructescences.

Effect of Bio-formulations on Qualitative and Quantitative Traits of Mulberry (Morus sp.) under Different Seasons

The experiments were conducted during, Kharif and Rabi (2018 - 2020) to investigate the effect of various bio-formulations on the quality and productivity of mulberry leaves. Among all the treatments, the maximum values of quantitative attributes such as shoot length (145.93 cm), internodal length (5.33 cm), number of leaves per shoot (35.04), fresh leaf weight per plant (719.05 g), dry leaf weight (241.28 g) and shoot yield (11.57 MT/ha/ harvest) were recorded in mulberry plants treated with EM @ 1 per cent in Kharif season. Besides, the biochemical constituents viz., total chlorophyll (2.80 mg g-1), carotenoids (0.824 mg g-1) crude protein (27.45 %), moisture (77.11 %), and moisture retention capacity (66.32 %) were notably higher in the EM treated mulberry leaves compared to control in Kharif season. The present study indicated that spraying EM @ 1 per cent on mulberry three times at 15 days interval during Kharif season helped improve the quality and yield of mulberry leaves compared to control.

Gamma Irradiation Enhanced Leaf Bioactive Components and Bioassay Parameters in M<sub>5</sub> Mulberry (<i>Morus</i> Sp.) Mutant

Gamma radiation is an effective tool for inducing genetic variation in plant characters. In the present experiment, M5 mulberry variety juvenile twigs were subjected to source Co60 gamma irradiation (1 kR - 10 kR) and mutants grown in triplicates in randomized block design to raise M1 and M2 generation. In M2 generation plants were subjected to phytochemical and bioassay tests. Silkworm rearing parameters and commercial characters of cocoons were recorded by feeding cross breed silkworms. Results show that M5 mutant leaves revealed significant variations in phytochemical constituents and moisture content. Bioassay tests recorded significant differences compared to control in M2 generation. Commercial characters like cocoon weight (1.41 g), shell weight (0.24 g), shell percentage (16.29 %), filament length (821.00 mts), renditta (8.2), denier (2.24) and effective rate of rearing (92.14 %) were increased. It is concluded that, gamma rays treatment enhances the mulberry plants leaf bioactive components, silkworm rearing and cocoon parameters and shows beneficial variants in M2 generation.

Microbial Changes in the Rhizosphere of Paper Mulberry (<i>Broussonetia papyrifera</i>) Mutant with a High Concentration of Crude Protein

Recently, many studies involving plant-microorganism relationships in the rhizosphere of multitudinous important economic crops revealed a clear signature of the host plant in shaping its rhizosphere microbial composition and structure. The nutrient preference of host plant was suggested to be one important factor determining the structure and assembly of the rhizosphere microbiome, but the proof for this hypothesis is still not enough. In this study, soil microbiomes in the rhizosphere of two Paper mulberry varieties with different nitrogen absorption and utilization efficiency were investigated using a short term pot experiment in controlled greenhouse, and the physicochemical properties were also determined. The results showed that, compared to the control plants, the mutated Paper mulberry variety with high N demand reduced the microbial growth significantly and changed the bacterial and the fungal composition in the rhizosphere soils, and alkaline nitrogen was identified to be the most significant factor affecting soil microbial community. Moreover, the effects of excessive consumption of soil nutrient during Paper mulberry cultivation on the microbiome was revealed, and it could be employed in field water and fertilizer management of Paper mulberry planting. This study further confirmed that the soil nutrient status resulting from the plant nutrient preference drives the development of a plant-specific microbiome.

Effects of Plant Extracts and Disinfectant Huva-San TR 50 on the Quality of Carrot (Daucus carota L.) Seeds

Abstract The aim of the study was to determine the effect of Aloe vera (L.) Burm.f. (Aloe) and Morinda citrifolia L. (Indian mulberry) plant extracts and disinfectant Huva-San TR 50 (hydrogen peroxide stabilized with silver ions) on carrot seed quality. The seeds of two carrot cultivars Amsterdam (sample I) and Berlikumer 2 (sample II), were soaked in solutions of Aloe and Indian mulberry extracts and Huva-San TR 50 at concentrations of 0.025 %, 0.05 % and 0.1 % for 30 min. Untreated seeds and seeds soaked in distilled water for 30 min were used as controls. Seed germination was assessed after 7 and 14 days of incubation. The speed and uniformity of seed germination (vigour) were evaluated. Mycological analysis was performed using a deep-freeze blotter test. Sample I was characterized by lower germination at the first and the final counts than sample II and higher seed infestation with Alternaria radicina. Treating seeds of sample I with Morinda citrifolia plant extract at concentrations of 0.025 and 0.05 % reduced their infestation with Alternaria alternata and A. radicina, increased germination at the first and the final counts, and did not affect seed vigour. The effects of Aloe extract and disinfectant Huva-San TR 50 on seed quality parameters varied depending on their concentration. Hence, further studies are necessary to establish the optimal conditions for carrot seed treatment.

Effects of Plant Growth Regulators on Fruit Set and Yield of Taiwan Morus macroura

In recent years, it is a common phenomenon that Taiwan Morus macroura has low fruit set in different mulberry planting areas that occurred popularly in China. This study aims to study the effects of plant growth regulators on fruit set and quality of Taiwan Morus macroura. Field experiments were carried out to investigate ten plant growth regulators, treated with 3 concentration gradients. The result shows that six plant growth regulators had better fruit preservation effect, the yield of mulberry which treated with plant growth regulators were significantly higher than that of control groups. To reserve fruit in fruit mulberry production, 85% 2,4-DNa SP with 400000~600000 dilution, 99.9% Boron fertilizer SL with 1500~2500 dilution, Amino acid soluble fertilizer SP with 35000 dilution, 80% DA-6 SL with 50000 dilution, 0.03% NAA SL with 50000 dilution and 8% Chlorophenoxyacetic acid sodium salt SP with 3000~6000 dilution are the best from the standpoint of food safety and economics.

Isolation and Characterization of Serratiopeptidase Producing Bacteria from Mulberry Phyllosphere.

Serratiopeptidase (EC 3.4.24.40), a proteolytic enzyme, is one of the most promising enzymes being used in biopharmaceutical industry. Mulberry phyllosphere, being an unexplored niche for exploration of protease production, was chosen for the present study. Protease producing bacteria were isolated from the tissues of mulberry plant as well as its rhizospheric soil. Two protease producing bacteria belonging to Serratia genus were found to be potential serratiopeptidase producers. Among them, the endophyte, i.e., Serratia marcescens MES-4 presented 95 Units/mL activity, while the soil isolate i.e., Serratia marcescens MRS-11 presented 156 Units/mL activity.

Potential of Mulberry Leaf Biomass and Its Flavonoids to Improve Production and Health in Ruminants: Mechanistic Insights and Prospects.

Simple SummaryThe economics of livestock production depends upon the feasible feeding resources as feed costs constitute more than 70% of the total expenses of a livestock enterprise. In this regard, searching for alternative and cheaper feeding resources is imperative for economical and sustainable livestock production. Mulberry leaves are an important resource available for feeding livestock, as they possess quite high protein and energy contents as compared to other tree leaves and conventional forages. Moreover, polyphenolic compounds (mainly flavonoids) present in mulberry leaf (ML) possess excellent antioxidant and antimicrobial potential that can beneficially impact animal health and production. Mulberry leaves and its flavonoids have been shown to increase the feed digestibility and milk production in ruminants, while reducing methane emission. Moreover, mulberry flavonoids can positively influence body metabolism and alleviate oxidative stress in animals. This review highlights the importance of this unique feeding resource for ruminants to increase their performance while reducing methane emissions.Leaf biomass from the mulberry plant (genus Morus and family Moraceae) is considered a potential resource for livestock feeding. Mulberry leaves (MLs) contain high protein (14.0–34.2%) and metabolizable energy (1130–2240 kcal/kg) with high dry matter (DM) digestibility (75–85%) and palatability. Flavonoid contents of MLs confer unique antioxidant properties and can potentially help alleviate oxidative stress in animals during stressful periods, such as neonatal, weaning, and periparturient periods. In addition, mulberry leaf flavonoids (MLFs) possess antimicrobial properties and can effectively decrease the population of ruminal methanogens and protozoa to reduce enteric methane (CH4) production. Owing to its rich flavonoid content, feeding MLs increases fiber digestion and utilization leading to enhanced milk production in ruminants. Dietary supplementation with MLFs alters ruminal fermentation kinetics by increasing total volatile fatty acids, propionate, and ammonia concentrations. Furthermore, they can substantially increase the population of specific cellulolytic bacteria in the rumen. Owing to their structural homology with steroid hormones, the MLFs can potentially modulate different metabolic pathways particularly those linked with energy homeostasis. This review aims to highlight the potential of ML and its flavonoids to modulate the ruminal microbiome, fermentation, and metabolic status to enhance productive performance and health in ruminants while reducing CH4 emission.

The dynamics in rhizosphere microbial communities under bacterial wilt resistance by mulberry genotypes.

The contribution of crops and soil microbial community structure and functional diversity in soil-borne diseases control mulberry plant production is still inadequately understood. In this work, a comparative study was undertaken on the microbial abundance, community structure, and functional diversity in the soil rhizosphere between the resistant (Kangqing 10) and the susceptible (Guisang 12) mulberry genotypes. The study deployed the use of dilution plate method, micro-ecology technology, and polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) techniques. The study aimed at developing better crop management methods for mulberry cultivation as well as preventing and controlling the occurrence and impacts of bacterial wilt on mulberry productivity. The results indicated that the soil rhizosphere microorganisms were more abundant in the normal resistant mulberry genotype than in the normal susceptible mulberry genotype. Carbon source utilization was better in the normal susceptible mulberry genotype. These properties were lower in the sickly resistant mulberry genotype than in the susceptible sickly mulberry genotype. Through the PCR-DGGE, it was shown that the bacterial and fungal community structures of the resistant genotypes were more stable than those of the susceptible genotypes. Through correlation regression analysis, it was shown that the mulberry bacterial wilt significantly contributes to the loss of soil nutrients, particularly organic matter and nitrogen, a possible cause to disrupted balance between the soil microbial community and the loss of soil organic matter. Resistant genotype plants displayed more resistance to bacterial wilt. Therefore, this study recommends the need to promote the cultivation of resistant genotype mulberry for increased yield.

Novel chemical degumming process and its effect on structure and properties of mulberry fibres

A novel chemical degumming (Fibre-N) method has been used to extract fibres from the bark of mulberry plant. Fibres obtained by a new method (Fibre-N) are characterized by the X-ray diffraction, scanning electron microscopy, Fourier transform Infrared spectroscopy, residual gum content, fibre yield, and mechanical property measurement. Fibre-N method exhibits 17.71% lower residual gum content, 37.18% higher breaking strength, 18.36% higher breaking elongation and 10.81% higher fibre yield. Fibre-N process used to extract fibres from mulberry plant is more economical and efficient, which could be used as a promising method for producing textile yarns, nonwovens, and composites.

The Effect of Morus Leaf Quality on Bombyx mori L. Nutrition Index

The effect of quality mulberry leaf Morus cathayana on nutrition index of silkworm Bombyx mori L. have been conducted . This research was Completely Randomized Design (CRD) by two treatments. The first treatment was mulberry plant that cultivated on soil with Urea, TSP and NPK fertilizers and the second was the plants were not fertilized l. Each treatment was replicate 20 times. The results showed that the addition of Urea, TSP and NPK fertilizer on mulberry has effect (p<0,05) on increasing of the growth rate (GR) of instar III, the consumption rate (CR) of instars III and V, and efficiency of conversion of ingested food (ECI) of instar III and V. In addition, approximate digestibility (AD) of instar III, IV were 20%, 7%, 24%, 40% and then V was 51%, 15%, 21% and 70% respectively.

The EFFECT OF THE QUALITY OF Morus cathayana LEAVES AGAINST NUTRITION INDEX Bombyx mori L.

The effect of quality mulberry leaf Morus cathayana on nutrition index of silkworm Bombyx mori L. have been conducted . This research was Completely Randomized Design (CRD) by two treatments. The first treatment was mulberry plant that cultivated on soil with Urea, TSP and NPK fertilizers and the second was the plants were not fertilized l. Each treatment was replicate 20 times. The results showed that the addition of Urea, TSP and NPK fertilizer on mulberry has effect (p<0,05) on increasing of the growth rate (GR) of instar III, the consumption rate (CR) of instars III and V, and efficiency of conversion of ingested food (ECI) of instar III and V. In addition, approximate digestibility (AD) of instar III, IV were 20%, 7%, 24%, 40% and then V was 51%, 15%, 21% and 70% respectively.

Impact of Bio-Accumulated Cadmium in Bombyx mori L. Reared on Mulberry Plants Grown in Heavy Metal-Impregnated Soils in Faisalabad, Pakistan

Industries in Faisalabad are discharging their wastewater containing different kinds of heavy metals without treatment into sewerages. This wastewater is used to irrigate different crops and trees. This study was planned to observe the entry of Cd from inorganic to organic source and its effects on the lifecycle of Bombyx mori (L.). Mulberry plants were irrigated with Cd-containing synthetic effluents of different concentrations (25 to 200 mg/l) at pH 5, and different levels of pH (3 to 5) at 100 mg/l, to check against different instars of silkworm. The amount of Cd in the samples of soil, mulberry leaves, silkworm larvae, silkworm faeces and silk glands were determined by using atomic absorption spectrophotometry (AAS). The results showed that B. mori can be used as an indicator of Cd pollutants because its body weight, body length and mortality rate was directly related to Cd concentration. Greater toxicity to silkworms was observed at higher accumulation of Cd in mulberry leaves. Maximum accumulation of Cd in soil, mulberry plants, silkworm larvae, silk glands and silkworm faeces was observed at 200 mg/l Cd and pH 5. The maximum accumulations observed were 99.89 mg/kg, 95.67 mg/kg, 61.32 mg/kg, 58.97 mg/kg and 6.59 mg/kg, respectively. Silkworms can be successfully used as an indicator of heavy metal pollutants and their role can further be marked with changes in the development.

Inhibitory effects of oxyresveratrol on ERK and Smad1/2 phosphorylation and HSC activation in preventing carbon tetrachloride-induced rat liver fibrosis

Oxyresveratrol (ORes, trans-2,4,3′,5′-tetrahydroxy stilbene) naturally exists in mulberry, grapes, peanuts and other plants. It belongs to stilbene polyphenolic family and has an extra hydroxyl group at 2-position comparing with resveratrol (Res). Hence, ORes has stronger antioxidant activity than resveratrol. In present study, we employed a rat hepatic fibrosis model induced by carbon tetrachloride (CCl4) and administrated ORes via gavage feeding to study the protective effects and potential mechanisms of ORes against hepatic fibrosis. We demonstrated that rat liver oxidative damage induced by CCl4 was significantly alleviated after ORes feeding. Furthermore, the mRNA transcription levels of α-smooth muscle actinn (α-SMA), desmin, and two MMPs (MMP2 and MMP9) were reduced and the expression levels of transforming growth factor β1 (TGF-β1), p- small mother against decapen-taplegic protein (Smad)1/2 and p-extracellular signal-regulated kinases (ERK)1/2 in the liver tissue down-regulated dramatically. In a parallel study with Res, ORes showed more efficacious protective effect than Res against rat liver fibrosis, which is attributed to extended conjugation system due to the extra hydroxyl group at 2-position on ORes making it more electron-rich and susceptible to oxidation than Res. Therefore, dietary consumption of mulberry and other fruits containing ORes may be beneficial in the prevention of liver fibrosis.

Physiological and transcriptomic analyses of mulberry (Morus atropurpurea) response to cadmium stress

Mulberry (Morus atropurpurea) is an economically important woody tree and has great potential for the remediation of heavy metals. To investigate how cadmium accumulates and its detoxification in mulberry, we assessed the physiological and transcriptomic effects of cadmium contamination and as well as its chemical forms and subcellular distribution. Cadmium significantly inhibited mulberry plant growth and primarily accumulated in mulberry roots. Antioxidant enzymes were induced by cadmium in all tissues of mulberry. Subcellular fractionation analyses of cadmium indicated that the majority was compartmentalized in soluble fraction in roots while it mainly located in cell wall in leaves and stems. The greatest amount of the cadmium was integrated with proteins and pectates in all mulberry tissues. RNA-seq transcriptomic analyses of mulberry roots revealed that various metabolic pathways involved in cadmium stress response such as RNA regulation, hormone metabolism, and response to stress, secondary metabolism, as well as signaling, protein metabolism, transport, and cell-wall metabolism. These results will increase our understanding of the molecular mechanisms of cadmium detoxification in mulberry and provide new insights into engineering woody plants for phytoremediation.

Characterization and Pulping of Crops Residue: Eggplant, Cassava, Okra and Mulberry Plants

In this paper, four crops residues such as eggplant, cassava, okra and mulberry plants were assessed as papermaking raw materials. These crops residues had lower α-cellulose and holocellulose and moderate lignin content with syringyl:guaiacyl range of 1.05–1.63. The fiber length of eggplant, cassava, and mulberry plants were shorter (0.58–0.65 mm), while the same for okra plant was medium (1.14 mm). The Runkle ratios of these plant fibers were low (0.208–0.678), and flexibility coefficient was low to medium (55–79). All these non-wood plants were difficult to cook except eggplant plant. Eggplant plant produced screened pulp yield of 33.21 with kappa number of 22.9 at the conditions 3 h cooking at 170 °C with 20 alkali charge. Cassava plant showed the lowest screened pulp yield of 17.7% with kappa number 30.3 at the conditions 2 h cooking at 170 °C with 20 alkali charge. The okra plant pulp showed the best papermaking properties among these crops residues. Eggplant and okra plants can be used for brown packaging grade pulp.

Identification and Characterization of Two Novel Geminiviruses Associated with Paper Mulberry (Broussonetia papyrifera) Leaf Curl Disease.

Paper mulberry (Broussonetia papyrifera) is a perennial woody plant used as source material for Cai Lun paper making, in traditional Chinese medicine, and as livestock feed. To identify the presence of viruses in paper mulberry plants affected by a disease with leaf curl symptoms, high-throughput sequencing of total RNA was performed. Analysis of transcriptome libraries allowed the reconstruction of two geminivirus-like genomes. Rolling-circle amplification and PCR with back-to-back primers confirmed the presence of two geminiviruses with monopartite genomes in these plants, with the names paper mulberry leaf curl virus 1 and 2 (PMLCV-1 and PMLCV-2) proposed. The genomes of PMLCV-1 (3,056 nt) and PMLCV-2 (3,757 to 3,763 nt) encode six proteins, with the V4 protein of PMLCV-1 and the V3 proteins of both viruses having low similarities to any known protein in databases. Alternative splicing of an intron, akin to that of mastre-, becurto-, capula-, and grabloviruses, was identified by small RNA (sRNA)-seq and RNA-seq reads mapping to PMLCV-1 and PMLCV-2 antisense transcripts. Phylogenetic analyses and pairwise comparisons showed that PMLCV-1 and PMLCV-2 are most closely related to, but distinct from, two unassigned geminiviruses, citrus chlorotic dwarf associated virus and mulberry mosaic dwarf associated virus, suggesting that they are two new members of the family Geminiviridae. Field investigation confirmed the close association of the two viruses with leaf curl symptoms in paper mulberry plants and that coinfection can aggravate the symptoms.

Utilization of wood vinegar as plant based insecticide in mulberry (Morus sp)

Successful maintenance of silkworms (Bombyx mori) depends on the supply of mulberry leaf feed (Morus sp). In practice, mulberry plants are not safe from pests and diseases. In the early rainy season, mulberry plants are easily attacked by whitefly (Trialeuroides vaporiorum and Bemissia tabaci). Mulberry leaves that are attacked by whitefly can no longer be given to silkworms because the nutrients are reduced. The use of plant-based insecticides in the resolvent of whitefly pests has not been widely practiced. Wood vinegar is an organic material derived from wood waste that can be used as a plant-based insecticide. The objectives of this study are to determine the percentage of whitefly attacks on mulberry plants and the application of plant-based insecticides on mulberry plants towards whitefly attacks reduction. The study was employed by field observation for 12 weeks. The results showed that the percentage of whitefly attack was 33.34%. Application of wood vinegar as insecticide with a dose of 2.5% (v/v) increased the pest resistance and induced the growth of mulberry plants.

Nutrition importance and health benefits of mulberry leaf extract: A review

Mulberry (Morus) belongs to the Moraceae plant family, native of China. The leaves of mulberry plant are the major food source for the silkworm. The leaf consists of minerals, vitamins, dietary fibre, amino acids, phytosterols, flavonoids and other functional components, mainly 1-DNJ (1-Deoxynojirimycin) which acts as an anti-diabetic drug. In countries like China, Korea and Japan the preparations from mulberry leaves are used as medicine for increasing the immunity of the human body, cough relief and to lower the blood sugar and blood pressure. The high nutritional value of leaves is used as a functional food, mostly in the form of herbal tea, smoothie, yoghurt, salads, nutritional masala biscuits, capsules, dry powder, oil, dietary supplement etc. This manuscript mainly focuses on the nutritional importance and health benefits of mulberry leaves extract. The leaves extract may help to lower blood sugar (diabetes), inflammation levels, cholesterol and fighting against heart disease.