Mucosal Irritation Research Articles

Role of Bioactive Ingredients from Medicine Food Homology Species in the Regulation of Chronic Gastritis

Backgrouds Chronic gastritis is a chronic inflammation of the gastric mucosa caused by various etiologies. Many studies in recent years have shown that certain Chinese medicines and their bioactive components possess potential therapeutic effects on chronic gastritis. Methods This review focused on promising medicine food homology species used in China for the prevention and management of chronic gastritis, and their bioactive ingredients and underlying mechanisms were summarized and discussed. Results Finally, flavonoids, saponins, terpenoids, alkaloids and polysaccharides were found to be effective in preventing and/ or managing chronic gastritis by combating Helicobacter pylori infection, reducing the inflammatory response and gastric mucosal irritation, and regulating the intestinal microbiota. Conclusions The review is valuable to future studies and the development of medicine food homology species for the management of chronic gastritis.

Ears, Nose, and Throat in Leukemias and Lymphomas

Leukemias and lymphomas, encompassing a spectrum of hematologic malignancies, often exhibit manifestations in various tissues and organs, including the ears, nose, and throat (ENT) region, extending beyond the typical sites of bone marrow and lymph nodes. This manuscript explores these interactions, considering disease-related symptoms and treatment effects. ENT symptoms, such as otalgia, hearing loss, and nasal obstruction, may arise from direct infiltration or treatment complications, with chemotherapy-induced ototoxicity being particularly characteristic. Furthermore, immunotherapy complications, including cytokine release syndrome and mucosal irritation, can also contribute to ENT symptoms. Additionally, targeted therapy and radiotherapy can lead to mucosal dryness, dysphonia, and radiation-induced otitis media. Patients with hematologic malignancies are especially vulnerable to various ENT infections, including bacterial, viral, and fungal infections, due to compromised immunity resulting from both the disease and its treatments. Conditions such as rhinosinusitis, otitis media, and pharyngitis pose significant management challenges. Moreover, patients undergoing hematopoietic stem cell transplantation (HSCT) face unique ENT considerations, including mucositis, opportunistic infections, and graft-versus-host disease in cases of allogeneic HSCT. These patients require specialized pre-transplant evaluations, meticulous post-transplant surveillance, and tailored assistance to mitigate complications. This manuscript underscores the importance of a multidisciplinary approach that integrates diagnostics, pharmacological interventions, and supportive care to address both disease-related and treatment-induced ENT manifestations. Further research is needed to refine management strategies and improve outcomes in this complex clinical population.

The Macedo ileum-based reservoir for the management of bladder involvement in epidermolysis bullosa.

Epidermolysis bullosa (EB) can severely affect the urinary tract, leading to strictures and urine outflow obstruction, which pose significant risks to kidney function. Procedures involving the urinary mucosa often exacerbate these issues, making safe bladder emptying a major challenge. This study reviews surgical methods for managing urological complications in EB patients, with a focus on the Macedo procedure, which offers a promising alternative that avoids further bladder mucosa irritation and prevents disease exacerbation. We conducted a systematic review of urological surgeries in EB patients using PubMed, Web of Science, and Google Scholar, adhering to PRISMA guidelines. We propose the Macedo procedure as a novel, safe, and effective technique for managing these patients' urological issues. The review revealed that most existing urological procedures for EB-related urethral obstruction often result in suboptimal outcomes, leading to permanent suprapubic catheters or vesicostomies. The Macedo ileum-based reservoir, with its continent stoma, significantly enhances the quality of life. By ensuring that catheters contact only the ileal mucosa, the procedure prevents exacerbation of EB lesions while maintaining efficient urine outflow and bladder drainage. We applied this technique to our EB patient with complete urethral obstruction following unsuccessful urethral dilations and a vesicostomy. The Macedo reservoir with a continent catheterizable stoma markedly improved the patient's quality of life, with promising initial outcomes despite limited follow-up. The Macedo procedure represents a valuable surgical option for catheterization in EB patients, reducing urothelial irritation and preventing disease progression, and has the potential to improve patients' quality of life.

Advantages of Nasal Drug Delivery System

The nasal drug delivery system has received a lot of interest as a non-invasive and effective way to provide medications. Its distinct benefits include quick absorption due to the nasal mucosa's extensive vascularization, bypassing first-pass metabolism, and increased bioavailability when compared to oral administration. This approach is especially useful for administering medications that have little gastrointestinal stability or are destroyed by hepatic enzymes. The nasal route offers an alternative to systemic medication administration, particularly for individuals who have difficulties swallowing pills or injections. Furthermore, the nasal cavity's closeness to the brain makes it an appealing alternative for treating central nervous system problems, as medications can traverse the blood-brain barrier via the olfactory and trigeminal pathways. Nasal administration is also simple, patient-friendly, and inexpensive, with minimum pain. Advances in formulation technology, such as nanoparticles and mucoadhesive compounds, have improved medication retention and effectiveness. Despite its potential, issues including mucosal irritation and low medication volume must be addressed. Overall, the nasal medication delivery system is a potential tool for increasing treatment results and patient compliance.

The use of glucocorticoids in Behçet’s disease

Behçet’s disease (BD) is a chronic, multifactorial inflammatory disorder characterized by episodic involvement of multiple systems, including mucocutaneous, ocular, vascular, gastrointestinal, joint, and neurological domains. Glucocorticoids (GC) play a pivotal role in managing BD, especially during acute flares and severe organ involvement. This review highlights the tailored use of GC across various manifestations of BD. For mucocutaneous lesions, topical GC are effective, where as short-term low-dose systemic GC are reserved for colchicine-resistantcases. In ocular BD, systemic GC are in dispensable for sight-threatening conditions, often combined with disease-modifying anti-rheumatic drugs (DMARDs) or biologics to minimize GC dependency. In vascular involvement, particularly pulmonary artery aneurysms, high-dose or pulse GC are essential to control vessel wall inflammation, often alongside immunosuppressive agents like cyclophosphamide. Neurological BD necessitate surgent high-dose GC therapy, complemented by DMARDs for sustained control. Joint involvement can be managed with intraarticular GC, reducing systemic exposure. In gastrointestinal BD, GC use is limited due to potential mucosal irritation, with biologics and DMARDs serving as adjunctive options. Across all manifestations, GC tapering is prioritized to mitigate adverse effects, while combination therapy with DMARDs or biologics ensures comprehensive disease control. This comprehensive review underscores the critical role of GC in BD management, advocating for individualized treatment strategies to balance efficacy and safety.

Protective effect of Dulaglutide, a GLP1 agonist, on acetic acid-induced ulcerative colitis in rats: involvement of GLP-1, TFF-3, and TGF-β/PI3K/NF-κB signaling pathway.

A chronic inflammatory condition of the colon called ulcerative colitis (UC) is characterized by mucosal surface irritation that extends from the rectum to the near proximal colon portions. The rationale of this work was to conclude if dulaglutide (Dula) could protect rats from developing colitis caused by exposure to acetic acid (AA). Rats were randomly divided into seven groups (each with eight rats): Normal control, Dula control, AA (received 2 milliliters of 3% v/v AA through the rectum), Sulfasalazine (SLZ); given SLZ (100 mg/kg) orally from day 11 to day 21 then AA intrarectally on day 22 and Dula groups ( pretreated with 50, 100 or 150 μg/kg subcutaneous injection of Dula - once weekly for three weeks and AA on day 22 to induce ulcerative colitis, colon tissues and blood samples were taken on day 23. By generating colonic histological deviations such as inflammatory processes, goblet cell death, glandular hyperplasia, and mucosa ulcers, Dula dropped AA-induced colitis. Additionally, these modifications diminished blood lactate dehydrogenase (LDH), C-reactive protein (CRP), colon weight, and the weight/length ratio of the colon. In addition, Dula decreased the oxidative stress biomarker malondialdehyde (MDA) and increased the antioxidant enzymes (total antioxidant capacity (TAC), reduced glutathione (GSH), and superoxide dismutase (SOD) concentrations). Dula also significantly reduced the expression of transforming growth factor-1 (TGF-β1), phosphatidylinositol-3-kinase (PI3K), protein kinase B (AKT) signaling pathway, and the inflammatory cytokines: nuclear factor kappa B (NF-κB), interleukin-6 (IL-6), and interferon-γ (IFN-γ) in colonic cellular structures. In addition, Dula enforced the levels of glucagon-like peptide-1 (GLP-1) and trefoil factor-3 (TFF-3) that were crucial to intestinal mucosa regeneration and healing of wounds. By modulating TGF-β1 in conjunction with other inflammatory pathways like PI3K/AKT and NF-κB, regulating the oxidant/antioxidant balance, and improving the integrity of the intestinal barrier, Dula prevented AA-induced colitis in rats.

Arginine Hydrochloride Reduce Rectal Mucosal Irritation of Sodium Aescinate: Molecular Docking, Physical Properties, Anti-Hemorrhoidal Activity, Safety and Topical Gel Formulations Investigation.

Background/Objectives: Sodium aescinate (SA) is commonly used topically due to its anti-inflammatory, anti-edematous, and anti-swelling properties. However, the clinical application of SA is limited by strong irritation, and cannot be used on the damaged skin and mucous membrane. This study aimed to investigate whether arginine hydrochloride (Arg·HCl) could reduce the rectal mucosal irritation of SA through the formation of a gel. Methods: Molecular docking was first used to explore potential interactions between SA and Arg·HCl. Gels for rectal administration were then formulated by combining SA with various ratios of Arg·HCl (from 1:0 to 1:10). In vitro tests, including pH, centrifuge stability, viscosity, and spreadability analysis, were conducted. The optimal gel formulation was determined based on rectal mucosal irritation tests and anti-inflammatory experiments. Additionally, the anti-hemorrhoidal characteristics and safety of the optimal gel in terms of acute toxicity and dermal sensitivity were evaluated. Results: The optimal SA to Arg·HCl ratio of 1:6 (F5-SA gel) was identified, significantly reducing rectal mucosal irritation while enhancing anti-inflammatory activity. The F5-SA gel demonstrated high efficacy against hemorrhoids, notably promoting anal ulcer healing. When administered rectally to rabbits at a dose of 132 mg·kg-1·d-1 (198 times the recommended therapeutic dose), no other obvious side effects were observed except a significant reduction in food intake on the day of administration. In addition, the gel did not induce dermal sensitivity. Conclusions: The F5-SA gel is a promising formulation that can reduce irritation and toxic side effects, and enhance the therapeutic effect to some extent, ultimately achieving a safer and more effective rectal delivery system for SA.

IMPACTO DA MUCOSITE ORAL EM PACIENTES TRANSPLANTADOS DE MEDULA ÓSSEA AUTÓLOGO E O PAPEL DO CIRURGIÃO DENTISTA

The Haematopoietic Stem Cell Transplantation (HSCT) is a form of treatment used for some types of hematological cancers in which the patient is both a donor and recipient. The most relevant side effect of this therapy for dentistry is oral mucositis, which in addition to causing mucosal irritation, delay and interruption of the chemotherapy cycle, can be a gateway to opportunistic infections and lead to a death outcome. As a dental surgeon increasingly active in the multidisciplinary team, the importance of dental care in the trans-HSCT phase has been widely reported in the literature. Thus, the general objective of the present work was to carry out a literature review on the impacts of oral mucositis in autologous bone marrow transplant patients, as well as the specific objective of highlighting the importance of the dental surgeon in the care of these patients. Thus, for the design of the research, the descriptors Stomatitis, Hospital Dental Team, Hematopoietic Stem Cell Transplantation, Autologous Transplantation, through DeSC; PubMed, Scielo and Lilacs databases and the Boolean operators AND, OR and NOT were used as a search strategy in the time period from 2004 to 2022. Concluding that the impacts of oral mucositis in patients undergoing auto-HSCT are pathophysiological, of morbidity, mortality and financial loss. However, the presence of the dental surgeon in the multidisciplinary team brought clinical relevance to reduce the impacts caused by oral mucositis, acting, even in a preventive way, with qualitative and quantitative reduction of the condition.

Synthesis of 2-methyl-6-methoxy-4-quinolinecarboxylic acid N-hydroxysuccinimide ester (MMQC-OSu) for streamlined and effective HPLC-based fluorescence detection of aliphatic amines in environmental samples

Aliphatic amines are widely distributed in the environment and food sources, posing potential health risks through skin and mucosal irritation. Consequently, their quantitative detection is crucial for assessing environmental health. Despite high reactivity and fluorescence properties of succinimidyl ester-based derivatization reagents, their application in the aliphatic amines detection is hampered by challenges such as limited detection sensitivity and fluorescence interference. We established an innovative synthetic approach to produce a series of succinimidyl esters with the desirable substituents. This advancement enabled the creation of efficient and highly sensitive reagents for the detection of aliphatic amines. Among them, 2-methyl-6-methoxy-4-quinolinecarboxylic acid N-hydroxysuccinimide ester (MMQC-OSu) exhibited the best detection performance. MMQC-OSu reacted with aliphatic amines at 40 °C in pH 8.0 buffer for 20 min, which was subsequently separated in a C18 chromatographic column with the fluorescence detection wavelength of 336/432 nm. This detection approach featured a rapid and mild reaction process, minimal interference from corresponding hydrolysis products, and impressive sensitivity (0.05 nM). These characteristics indicate that MMQC-OSu significantly surpassing commercial aliphatic amines detection reagents. Finally, the detection strategy of MMQC-OSu was successfully applied in environmental sample analysis, with recovery rate of 93 %–108 % and RSDs between 1.4 % and 6.5 %.

Removable dental prostheses for patients with Sjögren's syndrome

Sjögren's syndrome (SS) is a chronic autoimmune condition primarily affecting the salivary and lacrimal glands, leading to pronounced dry mouth (xerostomia) and dry eyes. These symptoms create unique challenges for SS patients requiring removable dental prostheses, as the lack of saliva significantly impacts the retention, comfort, and overall effectiveness of these devices. Without adequate saliva, prosthetic retention diminishes, and patients frequently experience issues such as mucosal irritation and increased susceptibility to infections. To address these issues, tailored material choices are critical in designing effective prosthetic solutions. Soft liners, moisture-retentive acrylics, and antimicrobial coatings are among the innovations improving both retention and comfort for SS patients. The aesthetic and functional outcomes of prosthetic solutions are also essential considerations. Enhanced retention mechanisms, such as implant-supported overdentures, not only improve masticatory performance but also aid in preserving the patient’s facial profile and lip support. Advanced materials offering translucency and color stability contribute to a more natural appearance, which is critical for patient satisfaction. The maintenance of these prostheses requires specific care routines to mitigate microbial buildup and tissue irritation. Specialized cleaning agents and salivary substitutes help maintain hygiene and reduce discomfort, while regular follow-up visits enable timely adjustments to accommodate tissue changes and extend the prosthesis lifespan. Implementing a comprehensive approach that considers material selection, functional and aesthetic outcomes, and maintenance strategies can greatly enhance the quality of life for SS patients. By understanding and addressing the specific needs associated with xerostomic conditions, dental professionals can provide prosthetic solutions that offer both reliability and comfort in this challenging context. Regular patient education and diligent care routines remain essential to ensure the long-term success and durability of removable dental prostheses for individuals with SS.

TEMPORARY REMOVAL: Exploring the potential of reconstructed human epithelial tissue models for safety assessment of intraoral medical devices

Medical devices are integral to a wide array of medical interventions and are increasingly utilized in both clinical and home settings. Within the oral cavity, intraoral medical devices are employed for various applications, to improve quality of life and maintain oral health and hygiene. However, the dynamic and complex environment of the oral cavity, characterized by the influence of factors, such as saliva composition, fluctuating pH, and microbial flora presents a challenge to ensure the safety of end-users. In this paper, we investigate the feasibility of utilization of 3D reconstructed human tissue models for the assessment of biocompatibility of intraoral medical devices. Building upon experiences drawn from the development and validation of ISO 10993-23 and from the development of a protocol for ocular irritation and photo-irritation, we suggest a new protocol for buccal mucosa irritation testing. The methodology is based on the viability assessment and analysis of cytokine release into media. By addressing intraoral medical devices biocompatibility testing, we aim to contribute to the advancement of biocompatibility assessment methodologies and increase the applicability of ISO 10993-23.

Determining the Effects of Nasopharyngeal Suction with Negative and Positive Pressure: Randomised Controlled Trial

Objective: The study aims to determine the effects of nasopharyngeal suction with negative and positive pressure on pain level, respiratory parameters, and mucosal irritation in infants. Methods: This study was conducted as a posttest randomized controlled experimental research. The data was collected at Göztepe Training and Research Hospital between January and November 2020. While the positive pressure suction method was applied to the experimental group, the negative pressure suction method was applied to the control group. Results: It is determined that the pain level mean scores at the 1st and 5th minutes after the suction procedure are lower in the experimental group than in the control group (p

STUDIES ON CHITOSAN GELS WITH POLYVINYL ALCOHOL TO PROTECT THE OESOPHAGEAL MUCOSA

Young children have an incompletely developed digestive tract and they are susceptible to numerous disorders due to the return of acid and alkaline content to the oesophagus. The aim of the study was to analyse the ability of hydrogels containing chitosan and polyvinyl alcohol to prevent irritation of the oesophageal mucosa in children. The addition of chitosan to the tested gels increased their pH, which could be useful to neutralise gastric reflux, and dynamic viscosity. The texture tests revealed that chitosan and polyvinyl alcohol significantly increased the work of adhesion work.

PROTECTION OF THE OESOPHAGEAL MUCOSA WITH CHITOSAN GELS

In developed countries, gastro-oesophageal reflux is one of the most frequently diagnosed diseases. Although acid reflux is more well known, alkaline reflux is equally troublesome. In either of the two cases, the oesophageal mucosa is destroyed. The aim of this study was to analyse the possibility of eliminating the problem by using hydrogels containing dextran and chitosan to prevent irritation of the oesophageal mucosa. The addition of chitosan to all tested gels increased their dynamic viscosity, enabling better adhesion and, consequently, better protection of the mucous membrane. The addition of dextran reduced the pH of the tested gels, which allowed for the neutralisation of alkaline reflux. Based on the texture tests, chitosan and dextran increased work of adhesion.

Acute toxicity of antiparasitic selamectin-based drug

The purpose of the research is to evaluate some toxicological parameters of antiparasitic selamectin-based drug.Materials and methods. The study drug is a solution for external use, 1 mL of which contains the active component selamectin (120 mg), as well as additives. The experiments used a total of 66 white outbred male rats, 42 white outbred male mice and 3 guinea pigs. The parameters of acute oral toxicity (in mice and rats), acute dermal toxicity (in rats), and dermal and mucous membrane irritation (in rats and guinea pigs) of antiparasitic selamectin-based drug were evaluated. LD50 was calculated and the irritation of the drug was evaluated using established procedures in toxicology.Results and discussion. The maximum tolerated dose of the selamectin-based drug was 2550 mg/kg in the mice and 4250 mg/kg in the rats. The absolutely lethal dose for the mice and the rats was 6800 and 8500 mg/kg, respectively. It was established that the drug belonged to hazard class 3 (moderately hazardous substances) when administered intragastrically to the animals: LD50 for the mice was 4816.7 mg/kg, and LD50 for the rats was 6091.7 mg/kg. In determining acute dermal toxicity in the rats, the drug was classified as hazard class 4 (low-hazardous substances): LD50 more than 8500 mg/kg. The drug irritant effect on the skin of the rats at doses of 4250, 6375 and 8500 mg/kg was not established. The drug had a mild effect on the mucous membrane of the eyes of the guinea pigs with its recovery to normal within 24 hours.

Biological, Antifungal, and Physical Efficacy of a Denture Cleanser Formulated with Cnidium officinale Extracts.

We aimed to assess the antifungal efficacy and impact of a denture cleanser containing Cnidium officinale extract on the surface characteristics of denture base materials, as well as its physical and biological properties. The experimental denture cleansers were formulated with C. officinale at concentrations of 100 and 150 μg/mL, combined with 1% cocamidopropyl betaine as a natural surfactant. Antifungal efficacy was evaluated using zone-of-inhibition assays against Candida albicans, revealing inhibition zones of 20 ± 1.8 mm for the 100 μg/mL concentration and 23.6 ± 1.6 mm for the 150 μg/mL concentration. Surface property assessments-including hardness, roughness, color stability, and solubility measurements-demonstrated no significant differences compared to the control group. Biological evaluations included the quantification of polyphenol and flavonoid content. The C. officinale-based cleanser showed significant antifungal activity without affecting the hardness, roughness, color stability, or solubility of denture base materials. Biological tests revealed no cytotoxicity and minimal mucosal irritation. Polyphenol and flavonoid contents were quantitatively measured, revealing higher concentrations in the experimental groups, which were correlated with significant antifungal activity. These compounds are known for their roles in disrupting microbial processes and enhancing antimicrobial effects. These findings suggest that the C. officinale-based denture cleanser effectively inhibits C. albicans while preserving the physical properties of denture base materials. This study highlights the potential of C. officinale in denture cleanser formulations, promoting denture hygiene and oral health. Future research should prioritize long-term clinical evaluations and formulation optimization.

Sedative-Hypnotic Effect and Mechanism of Carbon Nanofiber Loaded with Essential Oils of Ligusticum chuanxiong (Ligusticum chuanxiong Hort.) and Finger Citron (Citrus medica L. var. sarcodactylis) on Mice Models of Insomnia.

(1) Background: Insomnia is a neurological illness that poses a significant threat to both physical and mental health. It results in the activation of neuroglial cells, heightened neuroinflammation, oxidative stress, and disruptions in the Hypothalamic-Pituitary-Adrenal (HPA) axis. Ligusticum Chuanxiong (CX) and Finger citron (FC) are frequently utilized botanicals for addressing sleeplessness. Both herbs possess notable anti-inflammatory properties in their volatile oils. However, their effectiveness is hindered by the nasal mucosal irritation and instability they exhibit. (2) Methods: This study involved the preparation of a nanofiber composite system using carbon nanofiber (CNF) suspensions containing essential oils of Ligusticum chuanxiong-Finger citron (CXEO-FCEO-CNF). The effects and mechanisms of these essential oils in improving insomnia were investigated using an insomnia mouse model after encapsulation. (3) Results: The CXEO-FCEO-CNF had an average particle size of 103.19 ± 1.64 nm. The encapsulation rates of essential oils of Ligusticum chuanxiong (CXEO) and essential oils of Finger citron (FCEO) were 44.50% and 46.15%, respectively. This resulted in a considerable improvement in the stability of the essential oils over a period of 30 days. The essential oils effectively decreased the irritation of the nasal mucosa following encapsulation. Furthermore, CXEO-FCEO-CNF enhanced voluntary activity and sleep in mice with insomnia, notably boosted the activity of superoxide dismutase (SOD), reduced the concentration of lipoxidized malondialdehyde (MDA), decreased the levels of hormones associated with the HPA axis, and regulated the levels of neurotransmitters, resulting in a beneficial therapeutic outcome. CXEO-FCEO-CNF contains a total of 23 active ingredients, such as alpha-Asarone, (E)-methyl isoeugenol, and Senkyunolide. These ingredients primarily work by modulating the Janus kinase-signal transducer and activator of transcription (JAK-STAT) signaling system to decrease oxidative stress and inflammatory reactions. (4) Conclusions: This study presented initial evidence that the combination of CXEO and FCEO in nanofiber formulations effectively reduces the nasal mucosal irritation and instability of essential oils. Furthermore, it demonstrated the potential anti-neuroinflammatory and therapeutic effects of these formulations in treating insomnia. Overall, this study provides a theoretical foundation for developing new essential oil formulations derived from herbs.

Effectiveness of silver diamine fluoride (SDF) in arresting coronal dental caries in children and adolescents: a systematic review.

Dental caries remains a significant public health issue for children globally, leading to adverse effects on health and development. Silver diamine fluoride (SDF) is a potential preventive agent that can prevent caries progression in children. This systematic review examined the effectiveness of silver diamine fluoride in arresting caries compared to other fluorides. An electronic search of MEDLINE, PubMed, EMBASE, Cochrane, Web of Science, Scopus databases was carried out examining articles in English from 2001 to 2023. Studies included in the analysis examined the application of SDF in children and adolescents with coronal caries lesions on primary teeth or permanent first molars. Fifteen studies, involving a total of 7895 children, were incorporated. The application regimen varied across studies. Most studies in this review consistently suggested that SDF is effective in arresting caries. An annual application of SDF effectively reduced Streptococcus mutans count. Adverse effects were primarily tooth staining and less commonly, oral mucosal irritation. A majority of studies showed a high risk of bias due to methodological insufficiencies. Overall, the evidence suggests that SDF is effective in arresting dental caries in children. It offers a viable, cost-effective, and minimally invasive treatment option, particularly suitable for use in low-resource settings. However, the aesthetic concern of tooth staining with SDF use remains a challenge. Further well-designed clinical trials may provide a fuller picture of SDF which can shape public health policy and shift towards a minimally invasive treatment approach.

Development of an In-situ Nasal Gel Formulation of Bepotastine Besilate for the Treatment of Allergic Rhinitis

The objective of this research was to formulate and evaluate mucoadhesive in situ nasal gels as a novel delivery system for Bepotastine Besilate. Aiming to provide sustained drug release directly at the site of action, thereby reducing the need for frequent dosing due to the drug's rapid absorption and short half-life. A series of eight formulations were developed using varying ratios of Poloxamer 407 and Poloxamer 188. The formulations exhibited a visually clear sol phase, indicating uniform dispersion of ingredients, with gelling temperatures ranging from 33.3±0.41 to 36.7±0.73°C. The gelling time, an important parameter for user convenience and efficacy, ranged from 15.7±01.52 to 43.3±20.80 s, meeting practical application requirements. Furthermore, all formulations consistently achieved a drug content of over 95%, ensuring dosage uniformity and efficacy. The pH values of the formulations were within the acceptable range of 6.13±0.10 to 6.91±0.02, minimizing the risk of mucosal irritation upon application. Importantly, the mucoadhesive strength ranged from 1056±0.32 to 6456± 045 dyne/cm², indicating robust adhesion to nasal mucosa, which is critical for prolonged drug retention and sustained release. In vitro drug release studies demonstrated sustained release profiles exceeding 4 hours for all formulations, following Higuchi kinetics, suggesting controlled drug release from the gel matrix. Accelerated stability studies corroborated the formulations' stability over the test period, indicating their potential for long-term storage and commercial viability. Additionally, FTIR analysis revealed no evidence of drug-polymer interactions, confirming the compatibility of Bepotastine Besilate with the selected polymer matrix. These comprehensive findings support the potential of in situ nasal gels as an effective and promising strategy for enhancing the therapeutic efficacy of Bepotastine Besilate, thereby improving patient outcomes.

Clinical Study of Godhuma Yusha (Triticum Aestivum Linn.) in Amlapitta (Gastritis)

Gastritis is a condition that results from inflammation of the gastric mucosal layer characterized by swelling, pain and mucosal membrane irritation of the stomach. Gastritis in Ayurveda can be compared to Amlapitta and consumption of Godhuma in Ayurvedic classics is shown to be very effective. Objectives: To evaluate the study on Godhuma yusha in Amlapitta (gastritis) and assess its effects on Amlapitta (gastritis) Study Design: This was a randomized controlled study with 30 patients divided into two groups. Group A and Group B. Intervention: Group A included 15 patients and was administered 15ml of Godhuma yusha twice daily for a period of 3months. Group B also had 15 patients and was administered placebo 5gm twice daily. All the patients were followed at an interval of 15 days up to 3 months. Results: All the parameters such as Amlodgara, Hritkantadaha, Gourava, Utklesha, Avipaka and Agnimandya showed significant results in both the groups; Group A and Group B after the treatment compared to before treatment with p<0.0001 with more significant results in Group A compared to Group B with statistically significant results (p<0.001). Conclusion: Godhuma Yusha consumption was more effective compared to placebo in the management of Amlapitta.