Mucosa In Rat Model Research Articles

Mo1930 Beneficial Effect of Ursodeoxycholic Acid (UDCA) and Its Derivative UDCA-Cpa in Attenuation of Functional Impairment of Esophageal Mucosa in Rat Model of Barrett's Esophagus

Mo1930 Beneficial Effect of Ursodeoxycholic Acid (UDCA) and Its Derivative UDCA-Cpa in Attenuation of Functional Impairment of Esophageal Mucosa in Rat Model of Barrett's Esophagus

Microstructural changes of olfactory mucosa in rat model with acute rhinosinusitis leading to olfactory dysfunction

To observe the microstructural changes of olfactory mucosa in rat model with acute rhinosinusitis leading to olfactory dysfunction, and to provide foundation for further exploration of corresponding mechanism. On the basis of prior successfully established rat model of acute rhinosinusitis through inoculation with Streptococcus pneumoniae and with the help of merocel strips, one hundred healthy SD rats were randomly divided into experimental group (80) and control group (20). After inoculation, every 20 rats in the experimental groups were sacrificed in first week, second week, third week and fourth week respectively; and all rats in the control group were sacrificed in first week after the inoculation. Before the rats were sacrificed, the method called "buffed food pellet test, BFPT" was adopted, which was advanced by professor Nathan, to measure the rats' olfaction,and the time of every rat spending in finding out the food pellet was recorded and analyzed. BFPT showed that the rats in experimental group spent (402.9 ± 9.3), (453.7 ± 7.3), (351.9 ± 8.9), (278.7 ± 8.1) s respectively in searching the food pellet, which were more than the rats in the control group [(178.3 ± 6.6) s]. Then the olfactory mucosa was collected under anatomic microscope from all the rats to make frozen section and detect the changes of mature olfactory receptor neurons (ORN) and olfactory ensheathing cells (OEC) by immunofluorescence technique. The reduction of ORN in various degrees could be detected in the tissue samples of olfactory mucosa among all the rats in experimental group, with a tendency to become thinner in the thickness of epithelial lamina during the inflammation developing course. This kind of pathology was most marked in the second week and it gradually developed into the stage showing the lesion being the feeblest in the forth week following the beginning of modeling. Although the number of olfactory ensheathing cells appeared reduction in the first week following the beginning of modeling as well,it came to increase from the second week before olfactory receptor neurons and almost completely recovered to normal in the fourth week. In addition, some olfactory ensheathing cells could be detected in the tissue samples of olfactory mucosa among all the rats in experimental group. Both mature olfactory sensory neurons and olfactory ensheathing cells appeared to reduction when sinonasal mucosa taken place acute rhinosinusitis. But the number of olfactory ensheathing cells increased faster than olfactory sensory neurons. In addition, some olfactory ensheathing cells could be detected in the olfactory epithelium.

The effect of intranasal steroid on nasal mucosa in rat model of allergic rhinitis

To investigate the effects of intranasal steroid on nasal mucosa in rat model of allergic rhinitis (AR). Thirty-six SD rats were randomly divided into three groups (each included 12 rats): the intranasal steroid group(group A),the allergic rhinitis group(group B) and the normal control group(group C). AR model was reproduced by ovalbumin in group A and B. The control group was allergized by NS instead of ovalbumin. Group A was used steroid spray in nasal and Group B and C was used normal saline. Then ,after the last provocation,the allergic symptoms were evaluated,the number of eosinophil (EOS) of the rats' nasal mucosa was counted under hematoxylineosin staining and the goblet cells was counted under alcian blue-periodic acid-Schiff staining. The morphological changes of the rats' nasal mucosa were observed by scanning electron microscope. Compared with group B,the inhibition of nasal symptoms was found after used of steroid spray in group A. The EOS and the goblet cells in group A was significantly different with that in Group B (P < 0.05), but no significant differences compared with group C (P > 0.05). Observed by scanning electron microscope, the cilia of the epithelium in group B were significantly lodging, disoriented, winding and secreting,while the cilia of the epithelium in Group A and C had similar appearance. Intranasal steroid spray can relieve symptoms and inflammatory conditions of nasal mucosa in allergic rhinitis, and can repair injured nasal mucosa cilia.

Effects of paneth cells on restitution of intestinal mucosa in rat model of hemorrhagic shock

Objective To investigate the changes of Paneth cells during the restitution process of intestinal mucosa after hemorrhagic shock in rats. Methods A total of 42 Wistar male rats were randomized divided into control group ( n =7) and model group ( n =35 ). The rats in model group were further divided into five subgroups (n =7 in each group) according to different time points. Hemorrhagic shock model was established. The morphological changes of ileum mucosa and Paneth cells were observed during the restitution process after hemorrhagic shock, and the number of Paneth cells was counted under the light microscopy at different time points. Ultrastructures of Paneth cells were observed under different stages of resuscitation. Results After hemorrhagic shock, the mucosal epithelial injury was obvious in the upper villus and became more serious in 1 h up to 3 h, and mucosal restitution was found 6 h after resuscitation, almost established within 24 h. The number of Paneth cells in the intestinal mucosa was reduced significantly at 1 h after restitution (P ＜ 0. 05 ), reached the lowest at 3 h (P ＜ 0. 05 ), and gradually increased from 6 h after restitution (P ＞ 0. 05). Paneth cells showed ultrastructural features of intestinal endocrine cells.No apoptotic nuclear change was found. Conclusion Intestinal mucosa barrier was subjected to injury in the early stage of reperfusion after hemorrhagic shock, but it could recover rapidly. The synthesis and secretion of Paneth cell granules could be induced by ischemia-reperfusion injury of small intestine. Paneth cells played an important role in defensive mechanism of intestinal mucosa. Key words: Aneth cell; Intestinal mucosa; Morphology; Hemorrhagic shock

W1854 Treatment with Appetite Hormones Ghrelin and Leptin Exerts Beneficial Effect On Esophageal Mucosa in Rat Model of Reflux Esophagitis

W1854 Treatment with Appetite Hormones Ghrelin and Leptin Exerts Beneficial Effect On Esophageal Mucosa in Rat Model of Reflux Esophagitis

Fibrotic remodeling of nasal mucosa in rat model after radiation injury

To investigate the role of nasal mucosa fibrosis on radiation induced nasal mucosa injury. Seventy two male rats were randomly divided into two groups, control group and irradiation injured group (radiation dose were 40 Gy); the rats were killed 1 week, 2 weeks, 4 weeks, 8 weeks, 3 months and 6 months after the finish of radiation. The middle turbinates of the animals were removed. The pathological change of the nasal mucosa were observed with scanning electron microscope, transmission electron microscope, hematoxylin and eosin (HE), alcian blue-periodic acid-Schif (AB-PAS), and Masson Trichrome (MT). The Hyp content in nasal mucosa was measured with chemo-chromatometry. After radiation, the pathological characteristics in early stage (within 4 weeks) was acute inflammatory reaction. The repair of nasal mucosa started 4 weeks after radiation, lasted to 6 months. The deposition of collagen in nasal mucosa could be found 1 week after irradiation and increased gradually. Irradiation could induce a serials of pathological changes on nasal mucosa. The nasal mucosa fibrosis may be one of the reasons of persistent irradiation induced nasal mucosa injury.

Effects of cryptoporus polysaccharide on rat allergic rhinitis associated with inhibiting eotaxin mRNA expression

Aqueous extract from the fruiting body of Cryptoporus volvatus has been reported to present anti-tumor, anti-allergy, anti-inflammation and immunomodulatory activities. However, the effect mechanisms of anti-allergy and anti-inflammation are poorly understood. The aim of study is to evaluate whether Cryptoporus polysaccharides (CP) extracted from fruiting body of Cryptoporus volvatus decrease the development of nasal symptoms, airway hyperresponsiveness (AHR) to methacholine (MCh) and the infiltration of eosinophils in nasal mucosa in rat model of allergic rhinitis, and investigate a possible action mechanism of CP by detecting the expression of eotaxin mRNA in nasal mucosa and lung tissues. Rats were immunized with ovalbumin and consecutive topical antigen instillation was performed. Repeated intranasal ovalbumin challenge caused rhinitis symptom, AHR to MCh, eosinophil infiltration and histological alterations into the nasal mucosa and increase of eotaxin mRNA expression in nasal mucosa and lung tissue were examined. Pretreatment with CP 3, 9 and 27 mg kg −1 (ig) decreased the numbers of sneezing 27.4%, 38.4% and 44.3% and nasal rubbing 27.5%, 34.9% and 47.7% comparison with model group, respectively. CP caused a dose-related inhibition of MCh-induced AHR. CP 27 mg kg −1 decreased the expression of eotaxin mRNA in the nasal mucosa by 35%. These results suggest CP can relieve the symptom, eosinophil infiltration and injury of tissue in nasal mucosa and lung tissue and AHR of allergic rhinitis in rats. Its action mechanism may be associated with the decrease of eotaxin mRNA expression.