You have accessJournal of UrologyStem Cell Research1 Apr 2014PD8-08 A PORCINE MODEL OF TOTAL CYSTECTOMY AND URINARY REPLACEMENT BY TISSUE ENGINEERED URINARY DIVERSION USING A BIODEGRADABLE TUBULAR SCAFFOLD SEEDED WITH AUTOLOGOUS SMOOTH MUSCLE CELLS Trinity Bivalacqua, Elias Rivera, Gary Steinberg, Norm Smith, Manuel Jayo, Deepak Jane, Timothy Bertram, and Mark Schoenberg Trinity BivalacquaTrinity Bivalacqua More articles by this author , Elias RiveraElias Rivera More articles by this author , Gary SteinbergGary Steinberg More articles by this author , Norm SmithNorm Smith More articles by this author , Manuel JayoManuel Jayo More articles by this author , Deepak JaneDeepak Jane More articles by this author , Timothy BertramTimothy Bertram More articles by this author , and Mark SchoenbergMark Schoenberg More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2014.02.802AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES The aim of this study was to develop an incontinent urinary diversion (IUD) in a swine model of total cystectomy by surgically implanting biodegradable tubular scaffold seeded with autologous adipose derived smooth muscle cells from a product known as the Neo Urinary Conduit™ (NUC). METHODS Autologous smooth muscle cells (SMC) were isolated from adipose tissue obtained from Yorkshire pigs and seeded on biodegradable tubular scaffold (PLGA). Cystectomy was performed, ureters anastomosed to the NUC and peritoneum was used as blood supply to the NUC. In Group 1 (n=8), NUC was kept intra-abdominally and only the peritoneal wall was exteriorized to skin. In Group 2 (n=8), the peritoneum-wrapped NUC was exteriorized and carefully anastomosed to the skin to create a stoma. Repeated longitudinal measures of body weight, clinical chemistry, ultrasound imaging of kidneys/ureters, and histological examination using hematoxylin and eosin (H & E), Masson’s Trichrome, immunohistochemistry stains for AE1/AE3 (epithelium marker), CK7 (urothelium marker) and calponin 1 (smooth muscle marker) were performed 12 weeks post-implantation. RESULTS Peritoneum-wrapped NUC regenerated native-like urinary mucosa and muscularis layers in both groups. In Group 1, which used peritoneal tissue only to create the stoma an incomplete mucosal epithelium was formed and chronic inflammation developed around the stoma with narrowing at the skin. There was hydronephrosis of the kidneys and ureters. The left ureters were more severely affected. In Group 2, there was complete regeneration of an intact urothelium-to-epithelium transition characteristic of a native-like urethral meatus or muco-cutaneous junction. There was less hyronephrosis. Histological examination did not demonstrate ureteral narrowing at the NUC junction. In group 1, serum creatinine increased to 2.8 ± 0.9 from 1.2 ± 0.2 while in group 2, serum creatinine increased to 2.1 ± 0.4 from 1.3 ± 0.1. In both groups, there were distinct staining patterns in the NUC wall near the ureters and body which were positive for CK-7 and Calponin 1, indicating the presence of urothelium and smooth muscle bundles, respectively. CONCLUSIONS In a pre-clinical swine model, the NUC when seeded with autologous SMC regenerates into a native-like urinary conduit with an intact muco-cutaneous junction and full length uninterrupted urinary mucosal lining. Further investigations are necessary to determine durability of the regenerated urinary tissue and kidney function. © 2014FiguresReferencesRelatedDetails Volume 191Issue 4SApril 2014Page: e219-e220 Advertisement Copyright & Permissions© 2014MetricsAuthor Information Trinity Bivalacqua More articles by this author Elias Rivera More articles by this author Gary Steinberg More articles by this author Norm Smith More articles by this author Manuel Jayo More articles by this author Deepak Jane More articles by this author Timothy Bertram More articles by this author Mark Schoenberg More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...