MPV Values Research Articles

Anorectal Pathologies in the Course of Acute Leukaemias; Predictive Parameters.

Patients with leukaemia are exposed to infections as long as they are neutropenic. During this period, anorectal pathologies are among the common foci of infection with high mortality. In this study, we aim to investigate the factors that may have a predictive effect on early diagnosis and rapid intervention in perianal complications occurring in neutropenic patients diagnosed with leukaemia. A total of 90 patients with acute leukaemia, including 45 patients with anorectal pathology and 45 patients without anorectal pathology, were analysed. Demographics, blood group, BMI, haemogram and biochemical parameters at the time of diagnosis, and types of perianal pathology were recorded. In the group of patients with anorectal pathology, WBC, lymphocytes, monocytes, and LDH were significantly (p<0.05) higher, and platelets, MPV, and PCT were significantly (p<0.05) lower. The multivariate model showed significant-independent (p<0.05) efficacy of WBC and MPV values in differentiating patients with and without anorectal pathology. A significant efficacy was observed at the WBC cut-off of 17000 [area under the curve 0.656 (0.542-0.770)] and the MPV cut-off of 10 [area under the curve 0.667 (0.554-0.780)] in differentiating patients with and without anorectal pathology. Anorectal pathologies are common foci of infection in patients with acute leukaemia. Having predictive parameters that may help for early intervention will help the clinician. This is the first study in the literature to compare a control group with a group with anorectal pathologies in leukaemia patients providing a cut-off for WBC.

Evaluation of platelet concentrates prepared using different methods after overnight holding (18-24 h) of whole blood at room temperature.

Regulatory requirement of fixed holding time (6 h) of whole blood (WB) at room temperature, that is, 22-24°C (RT) results in sub-optimal component separation. The aim was to evaluate the platelet concentrates (PC) prepared by both platelet rich plasma (PRP) and buffy coat (BC) methods after overnight hold (18-24 h) at RT. A prospective experimental study was performed. A total of 48 WB units collected were divided into four groups (12 each) control-1 (C1) and test-1 (T1) for PRP and control-2 (C2) and test-2 (T2) for the BC method. Control groups were processed within 6 h, and in test groups, components were prepared after overnight hold, followed by evaluation of quality parameters. Irrespective of the method used, all PCs had similar volume, platelet yield, swirling, no bacterial contamination, RBC contamination, PaO2 and PaCO2 levels. PCs in the T1 group had significant differences in glucose and MPV values on d1, which were resolved by d5 of storage. PCs in T2 has significant differences in pH, glucose, and MPV levels throughout storage. PRBC in test and control groups had similar quality parameters till d42 of storage. FFPs in all tests were noninferior to the concurrent control groups till 3 months of storage. Overnight holding of WB had no lasting deleterious changes. Though a few biochemical parameters in the test groups were significantly different, they can be accepted to improve the logistics of component separation. Overall PRP method seemed to have a better result than the BC method after an overnight hold.

Mean Platelet Volume as a Predictive Marker for GDM

Background: Gestational Diabetes Mellitus (GDM) is defined as first onset of any glucose intolerance diagnosed in pregnancy and certain proportion has risk of developing type 2 diabetes later in life, but risk of type 1 is also increased. Screening at the earliest and enhanced diagnostic tools can help reduce burden of GDM and further progression as it is majorly a lifestyle disease. One of the easiest and cost-effective inflammatory markers are thrombocyte indices. Objective: To determine the potential value of MPV, an inexpensive and easy tool obtained from routine complete blood picture used to study morphology of platelet and whether it can be an indicator in prediction of GDM. Methods: Hospital based comparative study was done on 100 antenatal women attending OPD during first trimester. Case group includes women with recently diagnosed GDM as per guidelines of American diabetes Association by one-step 2-hour 75g OGTT and the cut off values were considered as per IADPSG. MPV is assessed amongst cases and controls and its association was studied in both groups. Results: The present study observed that MPV values of the GDM patients were significantly higher than those compared to normal pregnancies. The current investigation reveals that MPV values in GDM patients significantly exceed those observed in normal pregnancies. Specifically, the median MPV in the GDM group is 8.6 (IQR: 6.9-11.2), while in the non-GDM group, it is 7.4 (IQR: 4.8-10.4). The Mann-Whitney U test yielded a statistic of 905, with a corresponding p-value of 0.0175, which falls below the significance threshold of 0.05. Hence, there exists a statistically significant disparity in MPV between the groups. Conclusion: GDM is found to have underlying chronic inflammatory background and varied insulin resistance which states that these parameters may exist even before clinically diagnosable glucose intolerance occurs. This may direct that GTT might not detect GDM if done early in pregnancy, but inflammatory markers might diagnose as they have already been existing. One such economical marker is the MPV as a part of routine CBC. Keywords: Mean Platelet Volume; Gestational Diabetes Mellitus; IADPSG; Exclusive Real World Evidence

Protective Effect of Royal Jelly on Some Hematologic Parameters Against Sodium Arsenite Toxicity: A Study in Rats

This study examined the effect of royal jelly on some hematological values against sodium arsenite (NaAsO2)-induced toxicity in rats. Royal jelly is a known functional bee product; therefore, its potential to protect against these effects is of great importance. In this study, the effects of royal jelly on hematologic changes were investigated. In the study, 35 healthy Wistar albino male rats weighing 250–300 g and 12–13 weeks old were used. Rats were divided into different groups by gavage daily for 14 days. WBC, LYM, MID, GRA, RBC, HGB, MCHC, MCH, MCV, HCT, PLT, MPV, and PDW values were measured in blood samples. When the measured values were analyzed, the MID, RBC, MCHC, MCH, MCV, and PDV values did not show statistical significance in all experimental groups (P&gt;0.005). However, significant decreases in %LYM, %MID, HCT, and PLT values and increases in WBC, GRA, and %GRA counts were observed in all groups (P &lt; 0.005). This review shows that royal jelly has a potential effect on the hematological and immune systems against toxicity caused by harmful heavy metals such as arsenic. In particular, further research is required to identify the most effective dosage of royal jelly. The results highlight the protective potential of royal jelly against toxicity, which requires further investigation.

Complete blood count changes in sudden sensorineural hearing loss caused by Streptococcus suis bacterial meningitis at Prof. Dr. I.G.N.G. Ngoerah Hospital Denpasar from January 2019 – May 2023

Introduction: Sudden sensorineural hearing loss (SSNHL) is an emergency case that requires immediate and comprehensive management. This condition can be caused by Streptococcus suis bacterial meningitis. Complete blood counts (CBC) examinations are often carried out in SSNHL patients that caused by infection. Some CBC components can be clinical indicators of the severity and prognosis of SSNHL. Objective: To find out the differences in CBC among S. suis bacterial meningitis patients with SSNHL and S. suis bacterial meningitis patients with normal hearing at Prof. Dr. I.G.N.G. Ngoerah Hospital Denpasar from January 2019 – May 2023. Methods: This study used an analytical research design using a retrospective case control method to compare CBC results (hematocrit, NLR, PLR, MLR, RDW and MPV) in 40 cases patients with SSNHL due to S. suis bacterial meningitis and 40 controls patients with S. suis bacterial meningitis patients with normal hearing. Result: In this study, it was found that there is no significant difference between case group compared to control group in terms of CBC results. The mean hematocrit value was 40.67 (± 4.45) in cases and 40.87 (± 4.08) in controls, with p-value 0.842. The mean MPV value was 9.82 (± 1.58) in cases and 9.33 (± 1.78) in controls, with p-value 0.200. The mean NLR value in the case group was 24.46, while in the control group it was 28.79, with p-value 0.124. The mean PLR value in the case group was 200.01, while in the control group it was 247.80, with p-value 0.126. The mean MLR value was 1.33 in cases and 1.19 in controls, with p-value 0.303. The mean RDW value was 12.98 in cases and 12.81 in controls, with p-value 0.950. Conclusion: From 40 people suffering SSNHL due to S. suis bacterial meningitis it was found that most of research subjects are men (75%) and the average age of subjects is 53 years. This study found that there were no significant differences between the results of mean hematocrit, NLR, PLR, MLR, RDW and MPV in patients with SSNHL due to S. suis bacterial meningitis compared to controls.

Immunophenotyping of peripheral blood in NSCLC patients discriminates responders to immune checkpoint inhibitors

PurposeImmune checkpoint inhibitors (ICIs) dramatically changed the prognosis of patients with NSCLC. Unfortunately, a reliable predictive biomarker is still missing. Commonly used biomarkers, such as PD-L1, MSI, or TMB, are not quite accurate in predicting ICI efficacy.MethodsIn this prospective observational cohort study, we investigated the predictive role of erythrocytes, thrombocytes, innate and adaptive immune cells, complement proteins (C3, C4), and cytokines from peripheral blood of 224 patients with stage III/IV NSCLC treated with ICI alone (pembrolizumab, nivolumab, and atezolizumab) or in combination (nivolumab + ipilimumab) with chemotherapy. These values were analyzed for associations with the response to the treatment and survival endpoints.ResultsHigher baseline Tregs, MPV, hemoglobin, and lower monocyte levels were associated with favorable PFS and OS. Moreover, increased baseline basophils and lower levels of C3 predicted significantly improved PFS. The levels of the baseline immature granulocytes, C3, and monocytes were significantly associated with the occurrence of partial regression at the first restaging. Multiple studied parameters (n = 9) were related to PFS benefit at the time of first restaging as compared to baseline values. In addition, PFS nonbenefit group showed a decrease in lymphocyte count after three months of therapy. The OS benefit was associated with higher levels of lymphocytes, erythrocytes, hemoglobin, MCV, and MPV, and a lower value of NLR after three months of treatment.ConclusionOur work suggests that parameters from peripheral venous blood may be potential biomarkers in NSCLC patients on ICI. The baseline values of Tregs, C3, monocytes, and MPV are especially recommended for further investigation.

Effect of a Dietary Essential Oil Blend in Dairy Cows during the Dry and Transition Period on Blood and Metabolic Parameters of Dams and Their Calves.

Cows face severe challenges of immunosuppression and negative energy balance during transition periods. The current study was designed to investigate the effects of essential oil blend supplementation in dairy cow's ration during dry periods on the health of the cow and calf. In the current study, 45 dry Holstein dairy cows were divided into three groups, each consisting of 15 animals. The control group was without any supplementation; the positive control group was only injected with 0.2 mg/kg levamisole (intramuscular) at 2 months before parturition and 1 month before parturition; and the treatment group was supplemented with 3 g/day for each cow essential oil blend mixed in total mixed ration (TMR). A mixed vaccine against E. coli, Rotavirus, and Coronavirus was administered to all cows before 42 days and after 21 days of calving. The day of the calving of the animal was accepted as day 0. Blood samples were collected from the coccygeal vein of all cows at -60, -45, -30, -15, -7, 0, 1 and 2 days, and the colostrum samples were taken on days 0, 1, and 2. Blood samples were also collected from the jugular vein (V. Jugularis) of the newborn calves on days 0, 1, and 2. The results of the hematological parameters revealed no difference in the total red blood cell count, hemoglobin amount, hematocrit, MCV, MCH, MCHC, RDW, PLT, MPV, and PCT values in both prenatal and postnatal blood of the cows (p > 0.05). In terms of immunological parameters, the total IgG level was significantly higher in the experimental group on the 7th day before birth compared to the other two groups, while the total leukocyte count, lymphocyte count, monocyte count and granulocyte counts were found to be lower after birth (p < 0005). Blood NEFA and BHBA levels were also lower in the experimental group compared to the other two groups (p < 0.005), and the blood glucose level was not different (p > 0.05). In calves, no difference was found between the groups in any of the parameters examined (p > 0.05). It is concluded that the dietary addition of an essential oil blend during the transition period enhanced the immune status and energy metabolism of cows without any effect on the health status of newborn calves.

Relationship of intensive care scoring systems with neutrophil/lymphocyte ratio, platelet/lymphocyte ratio, and mean platelet volume values.

We evaluated the relationship between NLR, PLR, and MPV values and scoring systems frequently used in intensive care units in our study. In our retrospective study, patients aged 18 years and over who received treatment in the intensive care unit for at least 48 hours were included. Demographic data, such as age, gender, APACHE II, SOFA and GCS scores, expected mortality, and 30-day and 1-year mortality rates were recorded. There was asignificant positive correlation between MPV values and APACHE, SOFA, and expected mortality rates, and asignificant negative correlation between GCS values. It was also found to be significant that as the P/L ratio increased, APACHE, SOFA scores, and expected mortality rates decreased and GCS increased. In 30-day and 1-year mortalities, MPV values and CRP/albumin ratios were higher, and calcium values were significantly lower. The N/L ratios were also significantly higher in 1-year mortality. In our study, asignificant correlation was found between APACHE, GCS, SOFA, expected death rates and MPV and P/L rates. In conclusion, we suggest that in addition to intensive care scoring systems, the N/L ratio, P/L ratio, MPV, and CRP/albumin ratios can be used in the prognosis of patients (Tab. 5, Fig. 2, Ref. 18).

NLR、PLR、MLR、MPV在评估痛风性关节炎疾病活动度中的临床价值

NLR、PLR、MLR、MPV在评估痛风性关节炎疾病活动度中的临床价值

Hematological Profile in Dairy Cattle with Foot and Mouth Diseases in Lembang, West Bandung

Foot and mouth disease (FMD) is a widespread infectious disease that significantly affects the dairy farming sector in Indonesia. In reducing the impact caused by FMD, proper treatment performed by veterinarians is necessary. Hematological profile can be used as a data reference to prioritize dairy cattle infected with FMD. This study aimed to describe the hematological profile in dairy cattle with FMD infection. This study was collected data from 15 dairy cattle with FMD infection at Koperasi Peternak Sapi Bandung Utara (KPSBU), Lembang, West Bandung, West Java, Indonesia. Parameters observed in the hematology analyzer were the numbers of erythrocytes, leukocytes, lymphocytes, neutrophils, platelets, hemoglobin, hematocrit, MCV, MCH, MCHC, RDW, and MPV. The results of the examination of hematological profile showed various numbers. It found lymphopenia, neutrophilia, and high MPV values. It was suggested for veterinarians to refer to this study's results to determine an effective and efficient treatment.

Correlation of Tinnitus Severity Index and Tinnitus Handicap Inventory with Hematological Parameters in Patients with Subjective Tinnitus.

Introduction Tinnitus etiopathogenesis is still unclear and treatment options are controversial despite current advances in medicine. Objectives To analyze the correlation between patients' symptom scores, systemic inflammation, and trombosis biomarkers. Methods In this prospective study, we evaluated the degree of complaints of subjective tinnitus patients with the tinnitus severity index (TSI) and tinnitus handicap inventory (THI), and correlated these symptom scores with hematological parameters such as the neutrophil/lymphocyte ratio (NLR), platelet/lymphocyte ratio (PLR), mean thrombocyte volume (MPV), and platelet distribution width (PDW). Results A total of 44 patients with subjective tinnitus, 25 (56.8%) men and 19 (43.2%) women, were included in this study. The mean age of the patients was 42.3 ± 14.8 years. When the correlation between TSI and NLR, PLR, PDW, and MPV values of the patients were analyzed, no statistically significant correlation was found between TSI, NLR, and PLR ( p > 0.05). However, there was a statistically weak positive correlation between TSI, MPV, and PDW.( p < 0.05). When the correlation between THI and NLR, PLR, PDW, and MPV values of the patients were analyzed, no statistically significant correlation was found between THI, NLR, and PLR ( p > 0.05). There was a statistically weak positive correlation between THI, MPV, and PDW ( p < 0.05). Conclusion We were unable to detect any relationship between systemic inflammation markers (NLR and PLR) and symptom scores, but a weakly positive correlation was observed between thrombosis markers (MPV and PDW) and symptom scores, and as the subclinical thrombosis markers elevated, so did the symptom scores.

Mean Platelet Volume as a Prognostic Marker in Sepsis in ICU

Abstract Background Sepsis is defined as a systemic inflammatory response to confirmed or suspected infection. It is a major cause of morbidity and mortality, and the incidence is rising, probably due to the growing elderly population, antibiotic resistance, immunosuppressive medication and, invasive surgery. Pneumonia is the most common infection leading to sepsis, followed by urinary tract infections and abdominal infections. These infections are usually localized and controlled by the immune system, but they can sometimes spread and cause sepsis. Objective To correlate between the mean platelet volume in patients with sepsis with C-reactive protein as a prognostic markers. Patients and Methods This is a prospective study conducted on 80 adult critically ill patients of both sexes with sepsis and sever sepsis who admitted to the units of Critical Care Medicine Ain Shams University Hospitals. The samples were collected during the period from 1st October 2019 to 31st March 2020, MPV results were correlated to CRP, SOFA score and APACHE score. Results The results of the present study revealed: 55.8 % were male and 44.2 % were females. Sex did not affect mortality, no statistical significance found. In this study, MPV was a significant parameter in septic patients. The cut off value for MPV was 10.5 fl which was found to be significant and of a magnitude considered to be clinically useful, with sensitivity 57.1%, specificity 80.8%, p value (0.003) and accuracy 72.5% in predicting mortality. A correlation was found between high MPV, CRP, SOFA and APACHE scores. Therefore, MPV may be used as an auxiliary test in the prognosis of sepsis. Conclusion Initial values of MPV were significantly correlated with CRP, APACHE and SOFA scores and with the estimated rate of mortality.

Platelet and erythrocyte parameters in type 2 diabetic moroccan patients: A cross-sectional study

Objectives: Complete blood count parameters have gained renewed interest as predictors of endothelial dysfunction in type 2 diabetes mellitus (T2DM). Thus, the aim of this study was to observe the changes in platelet and erythrocyte parameters between diabetics and nondiabetics and to evaluate these changes in relation to glycemic control. Materials and Methods: This cross-sectional study was performed in Mohammed VI University Hospital, Marrakech, Morocco, from January 2020 to July 2021. A total of 307 Moroccan patients were enrolled in this study, which included 222 diabetic patients and 85 nondiabetics. The diabetic patients were divided into two groups A (hemoglobin A1c [HbA1c] ≤ 6.5%) and B (HbA1c &lt; 6.5%) according to their glycated hemoglobin levels (HbA1c). Biological parameters were processed as per the standard technique. Data analysis was performed using SPSS statistical software. Statistical Analysis: Data were analyzed using SPSS 23 software (Statistical Package for Scientific Studies; IBM Corp. Armonk, New York, United States), and it was described as mean and standard deviation. Results: A significant difference was mentioned regarding mean corpuscular volume (MCV) (p &lt; 0.001), red blood cell distribution width (RDW) (p &lt; 0.001), and volume/platelet count (p ¼ 0.023) between diabetics and nondiabetics. MCV and RDW were lower in group B than group A (p ¼ 0.004; p ¼ 0.04), while MPV was higher (p ¼ 0.342). RDW significantly and negatively correlated with HbA1c in diabetic population (r ¼ –0.182; p ¼ 0.006). On the other hand, a significant and positive correlation between MPV and HbA1c was mentioned (r ¼ þ0.184; p ¼ 0.006). Conclusion: Our study showed that RDW and MPV correlate with HbA1c in T2DM and can be used as a reproducible and inexpensive means to ensure proper control of glycemic status. Further studies are warranted to define specific RDW and MPV values predictive of complication risk in the diabetic population.

Role of C reactive protein, hyperbilirubinemia and mean platelet volume in diagnosis of acute appendicitis and its correlation post operatively with histo-pathological examination

Background: Acute appendicitis is one of the most common abdominal emergencies requiring emergency surgery. The diagnosis based on the onset of symptoms and physical examination. Therefore, inaccuracies caused by subjective interpretation are frequent. This raises the need for tests to help diagnose acute appendicitis and predict the severity of the condition. aim of the study to investigate whether mean platelet volume, C-reactive protein, and hyperbilirubinemia can be used as inflammatory markers for the diagnosis of acute appendicitis and its correlation with histopathological examination. Methods: we prospectively analyzed 60 patients who were full fulling the inclusion criteria patients analysed for CBC, LFT and CRP and underwent for appendicectomy (Laparoscopic/open). Per-operative diagnosis was confirmed by histopathological examination from November 2020 to November 2022. Results: patients with CRP level &gt; 6 and hyperbilirubinemia patients most likely to have acute appendicitis. Mean CRP level was 13.21±4.0 and mean bilirubin level 1.04±0.29 in case of acute appendicitis. CRP, hyperbilirubinemia levels were significantly higher in cases of acute appendicitis (p&lt;0.01) while MPV value was not found significant. All patients was confirmed with histopathology examination. Conclusions: Serum bilirubin level and CRP estimation, which is a simple, cheap, and easily available laboratory test, can be added to the routine investigations in the diagnosis and severity of acute appendicitis and help in decreasing negative appendicectomy rates, especially in low modified Alvarado score.

#5319 ACCURACY OF PLATELET INDICES AS PREDICTOR OF IN-HOSPITAL MORTALITY IN PATIENTS WITH ACUTE KIDNEY INJURY REQUIRING RENAL REPLACEMENT THERAPY

Abstract Background and Aims Acute kidney injury (AKI) is a major medical complication associated with a markedly increased risk of death, particularly in patients admitted to the ICU where in-hospital mortality exceeds 50%1. This study aimed to determine the accuracy of MPV, platelet count, and PDW as predictors of adverse outcomes in patients with AKI who received initiation renal replacement therapy (RRT). Method A retrospective cohort single-center study was done in a local private tertiary hospital in Cebu. Four hundred ninety-one patients with AKI who underwent RRT between January 2018 and December 2021 were enrolled. A retrieval of data on demographic and clinical parameters during the initiation of RRT was done. The impact of mortality-related factors were identified using univariate and multivariate logistic regression analysis. Determination of optimal cut-off values of platelet indices for in-hospital mortality was done. Results This study showed that the in-hospital mortality of patients was 58.45%, with a mean age of 68.6 ± 16.28 years among non-survivors. Among the platelet indices, platelet count and PDW were good predictors of in-hospital mortality in patients who received initiation renal replacement therapy. The optimal cut-off value of platelet count was 173 × 103/uL (sensitivity 56.45%, specificity 62.25%, PPV 67.78%, NPV 50.40%, AUC 0.604). The optimal cut-off value of PDW was 16.45% (sensitivity 50.87%, specificity 71.57%, PPV 71.57%, NPV 50.87%, AUC 0.611). Conclusion Platelet indices are feasible parameters that can be used as prognostic markers for mortality in patients with AKI requiring RRT. The in-hospital mortality of patients with AKI requiring initiation RRT is high (58.45%). Low platelet counts, high MPV values, and high PDW values are associated with poorer outcomes and higher mortality risk as compared to patients with normal indices.

#5304 ACCURACY OF PLATELET INDICES AS PREDICTOR OF IN-HOSPITAL MORTALITY IN PATIENTS WITH ACUTE KIDNEY INJURY REQUIRING RENAL REPLACEMENT THERAPY

Abstract Background and Aims Acute kidney injury (AKI) is a major medical complication associated with a markedly increased risk of death, particularly in patients admitted to the ICU where in-hospital mortality exceeds 50%1. This study aimed to determine the accuracy of MPV, platelet count, and PDW as predictors of adverse outcomes in patients with AKI who received initiation renal replacement therapy (RRT). Method A retrospective cohort single-center study was done in a local private tertiary hospital in Cebu. Four hundred ninety-one patients with AKI who underwent RRT between January 2018 and December 2021 were enrolled. A retrieval of data on demographic and clinical parameters during the initiation of RRT was done. The impact of mortality-related factors were identified using univariate and multivariate logistic regression analysis. Determination of optimal cut-off values of platelet indices for in-hospital mortality was done. Results This study showed that the in-hospital mortality of patients was 58.45%, with a mean age of 68.6 ± 16.28 years among non-survivors. Among the platelet indices, platelet count and PDW were good predictors of in-hospital mortality in patients who received initiation renal replacement therapy. The optimal cut-off value of platelet count was 173 x 103/uL (sensitivity 56.45%, specificity 62.25%, PPV 67.78%, NPV 50.40%, AUC 0.604). The optimal cut-off value of PDW was 16.45% (sensitivity 50.87%, specificity 71.57%, PPV 71.57%, NPV 50.87%, AUC 0.611). Conclusion Platelet indices are feasible parameters that can be used as prognostic markers for mortality in patients with AKI requiring RRT. The in-hospital mortality of patients with AKI requiring initiation RRT is high (58.45%). Low platelet counts, high MPV values, and high PDW values are associated with poorer outcomes and higher mortality risk as compared to patients with normal indices.

Diagnostic performance of routine blood parameters in periodic fever, aphthous stomatitis, pharyngitis, and adenitis syndrome.

We aimed to investigate the difference between PFAPA and streptococcal tonsillitis (Strep Pharyngitis) by using blood parameters. We want to evaluate the relationship between periodic fever, aphthous stomatitis, pharyngitis, adenitis (PFAPA) syndrome, and tonsillitis by using NLR. The data of 141 pediatric patients who had applied to our clinic between October 2016 and March 2019 and were diagnosed with PFAPA syndrome and tonsillitis were reviewed from hospital records. The demographic data of the study group were recorded, as were their WBC, neutrophil, and lymphocyte counts, NLR, and MPV values, which are obtained by proportioning these two counts. CRP and ESR values were significantly higher in the PFAPA group (p = 0.026 and p < 0.001, respectively). No significant difference was determined between the groups in terms of platelet count or lymphocyte count. Receiver operating curve analyses were calculated. The AUC was 0.713 ± 0.04 according to age, and the CRP was 0.607 ± 0.04 (95% confidence interval). Using a cutoff point of >49 months for age, the sensitivity was 0.71 and the specificity was 0.67. With simple laboratory parameters, PFAPA syndrome can be differentiated from a diagnosis of tonsillitis. This may reduce the costs associated with unnecessary antibiotic use. However, these findings still need to be confirmed by other future studies.

Does complete blood count have a role in the prediction of deep vein thrombosis?

It was planned as a retrospective case-control study in which 1,527 patients between January 2022 and September 2022 were evaluated. After the eligibility criteria, systematic sampling was performed and analyzed in the case group (103) and the control group (179) patients. The predictive significance of Hb, NLR, PLR, MPV, PLT, MPV/PLT, monocytes, lymphocytes, eosinophils, RDW, LMR, and PDW parameters for the development of DVT was investigated. Then, logistic regression analysis was performed with these parameters to analyze the predictive value. The cutoff point was determined by performing ROC analysis for the statistically significant parameters. Neutrophil, RDW, PDW, NLR, and MPV/platelet values were statistically higher in the DVT group than the control group. Lymphocyte, PLT, and LMR values were statistically lower in the DVT group than the control group. There was no statistical difference between the two groups in terms of neutrophils, monocytes, eosinophils, Hb, MPV, and PLR values. RDW and PDW values were statistically significant for DVT prediction (p < 0.001, OR = 1.183 and p < 0.001, OR = 1.304, respectively). According to ROC analysis, 45.5fL for RDW and 14.3fL for PDW were determined as the cutoff points for DVT prediction. We found RDW and PDW to be significant in terms of DVT prediction in our study. We found the NLR and MPV/PLT to be higher in the DVT group, and the LMR to be lower in the DVT group, but we found that there was no statistically significant predictive value. CBC can be used as an inexpensive and easily accessible test that has predictive significance for DVT. In addition, these findings need to be supported by prospective studies in the future.

A Rare Immunodeficiency As a Cause of Inflammatory Bowel Disease; ARPC1B Deficiency

Actin-related protein 2/3 complex subunit 1B (ARPC1B) is involved in the control of actin polymerization. Homozygous loss-of-function mutations in ARPC1B cause immunodeficiency 71 classified as the syndromic combined immunodeficiency with congenital thrombocytopenia. Here we describe two siblings presented with homozygous mutations in the ARPC1B gene. Patient 1, a 12-year-old male, presented with bloody diarrhea, vomiting, fever and eczema starting at the age of five months. GIS endoscopic examination was compatible with Chron’s Disease. He had repeated hospitalizations due to fever, bloody diarrhea, vomiting, pneumonia, asthma, and bronchiolitis. Physical examination was normal except for marked growth retardation. Complete blood count (CBC) revealed thrombocytopenia with normal MPV values, lymphopenia as well as eosinophilia. He had high Ig A, low Ig M, and normal Ig G and Ig E levels. In the lymphocyte subgroup analysis, CD3+, CD8+ T, CD 19+ B cell and RTE percentages were low, and CD21+38+ low B cell percentages were high. Lymphocyte proliferation and Treg cell analysis were normal compared to healthy controls. Patient 2, Patient 1’s three-year-old brother started to complain of vomiting, bloody diarrhea, and eczema when he was four days old. Colonoscopy was revelaed Crohn’s Disease. He had episodes of wheezing and repeated hospitalizations for bronchiolitis. Physical examination revealed growth retardation and severe eczema. CBC was normal except for intermittent leukocytosis and eosinophilia. He had high Ig A and Ig E, low Ig M, and normal Ig G levels. In lymphocyte subgroup analysis, CD3 + T cell, RTE, naive, and CSM B cell percentages were low, CD21+38+ low B and Exhausted Cd8+ T cell percentages were high. T cell proliferative response was significantly lower than that in healthy controls. Treg cell analysis was also found to be significantly lower.Homozygous splicing mutation in the ARPC1B gene was detected in the WGS analysis of the patients. They are preparing for hematopoietic stem cell transplantation.IBD is an important finding of immune dysregulation. Advances in next-generation gene sequencing are revealing the underlying immunodeficiencies of IBDs. ARPC1B identified in our patients is an example of this. Diagnosis of patients reduces mortality and morbidity by providing new treatment options.

Hematological and biochemical markers in determining the diagnosis and stage prediction of endometrial cancer.

To establish whether there is a statistically significant difference in hematological and biochemical parameters between the patients with premalignant changes of the uterine mucosa and those with malignant changes. The aim is to establish whether hematological and biochemical parameters may be useful in predicting the stages of endometrial malignancy and in differentiating premalignant and malignant endometrial changes. A retrospective study included 100 patients (70 with endometrial carcinoma diagnosis and 30 with atypical hyperplasia). We compared hematological and biochemical parameters in both groups. CRP, granulocytes, platelets, neutrophil-lymphocyte ratio (NLR), platelet-lymphocyte ratio (PLR) are statistically significantly higher in patients with malignant changes. Lymphocyte count is statistically significantly lower in patients with malignant changes. Platelet count is statistically significantly lower in patients with stages I and II in comparison to patients with higher disease stage. NLR and PLR have good discriminatory power for carcinoma presence. Patients with advanced changes have statistically significantly higher CRP values, higher granulocyte and platelet count, as well as higher values of NLR and PLR, and statistically significantly lower values of lymphocytes and MPV in comparison to benign changes. There is a possibility of using hematological and biochemical parameters in the assessment of endometrial changes as well as in the prediction of stages, in confirmed malignant changes of the endometrium.