Objectives: Drop jump (DJ) is a typical exercise of plyometric training in which the state before takeoff (pre-set phase) influences the force exertion of the lower limbs during takeoff, as well as performance variables. This study aimed to examine the effects of watching an instructional movie during the pre-set phase on the performance and lower limb kinematic and kinetic variables during plyometric training.Methods: Fourteen participants (age, 21.9 ± 2.1 years; height, 175.6 ± 2.6 cm; weight, 70.7 ± 4.9 kg) were enrolled in this study. Seven participants with a high rebound jump (RJ) index under normal conditions were classified into the high RJ-index group. Seven participants with a low RJ-index were classified into the low RJ-index group. DJs were performed under normal conditions and under the movie condition (DJ immediately after watching the instructional movie during the pre-set phase). Performance and kinematic and kinetic variables of the lower limb joints were measured.Results: Compared with the normal condition, the movie condition was associated with a significantly high RJ-index, lesser degree of knee flexion (p < 0.011), and significantly larger concentric torque (p < 0.018) of the ankle. An interaction effect was observed for the eccentric torque (p < 0.025) and positive power (p < 0.004) of the ankle, which were significantly greater in the high RJ-index group under the movie condition.Conclusion: Watching an instructional movie during the pre-set phase improves the movement and force production of the ankle and knee joint, which, in turn, improves the DJ performance. However, the effects may be more pronounced in participants with a high RJ-index.