With the continuous improvement and maturity of mobile robot technology, a wheeled robot has been applied in many fields, such as military, industrial, service, transportation, etc., and has become an important tool to assist or replace people to complete specific tasks. In general, the working environment of a wheeled mobile robot is mostly outdoor, and the environment it faces is relatively complex, which restrains the autonomous movement and remote operation of the robot. Therefore, in this paper, an omnidirectional mobile robot is applied in a complex environment and a digital elevation model is introduced to assist autonomous movement and positioning of the robot. From the perspective of the application effect, the mobile robot integrated with the digital elevation model can operate and locate in the complex environment without GPS. While confirming the integration of the digital elevation model, the effect of autonomous operation and location of the mobile robot in the complex environment is also clarified. The purpose of this study is to improve the motion control and autonomous positioning ability of mobile robots in complex environments and promote the development of mobile robots.