Mouse Splenic Dendritic Cells Research Articles

IL-10 inhibits the primary allogeneic T cell response to human peripheral blood dendritic cells.

Most of the immunosuppressive properties of IL-10 are related to its action on antigen presenting cells (APC). Until now, several mechanisms have been described by which IL-10 inhibits the ability of monocytes/macrophages to induce T cell activation. Indeed, IL-10 down-regulates their expression of MHC class II molecules1 as well as of ICAM-1 and B7-1 accessory molecules2,3. Moreover, the inhibition by IL-10 of IL-12 and IL-1 synthesis by monocytes was shown to be responsible for the inhibition of IFN-y production by peripheral blood mononuclear cells (PBMC)4. As far as the effects of IL-10 on dendritic cells are concerned, IL-10 was shown to inhibit the ability of mouse splenic dendritic cells to induce interferon (IFN)-y production by antigen-specific TH1 clones or alloreactive splenic T cells5. In addition, human epidermal Langerhans cells preincubated with IL-10 were found to be deficient in the triggering of a mixed lymphocyte reaction6. As a first approach to study the effects of IL-10 on human peripheral blood dendritic cells (PBDC), we analyzed the influence of IL-10 on a primary allogeneic T cell response induced by those cells.