Mouse Proximal Tubule Cells Research Articles

Acid activation of immediate early genes in renal epithelial cells.

These studies examined the effect of acidosis on immediate early (IE) gene expression in renal tubule cells. In MCT cells, an SV40 transformed mouse proximal tubule cell line, incubation in acid media led to transient increases in c-fos, c-jun, junB, and egr-1 mRNA abundance, peaking at 30 min to 1 h. In vivo metabolic acidosis caused more prolonged increases in these mRNA species in renal cortex. Nuclear runon studies demonstrated increased rates of transcription for these IE genes. In addition, pretreatment of cells with cycloheximide caused superinduction of these mRNA by acid incubation. These responses are similar to those elicited by growth factors. Inhibition of tyrosine kinase pathways prevented IE gene activation by acid, while inhibition of protein kinase C and/or increases in cell calcium had no effect. In 3T3 cells, acid activated IE genes by a different mechanism in that the increase in mRNA did not include c-jun, was more prolonged, and was blocked by cycloheximide. In summary, incubation of renal cells in acid media leads to activation of IE genes that is similar to growth factor-induced IE gene activation, and is likely mediated by tyrosine kinase pathways.

P-268 - Establishment of mouse proximal tubule cell line maintaining a wild character, ammoniagenesis

P-268 - Establishment of mouse proximal tubule cell line maintaining a wild character, ammoniagenesis

Hepatocyte growth factor stimulates phosphoinositide hydrolysis and mitogenesis in cultured renal epithelial cells

Hepatocyte growth factor (HGF), a novel heparin-binding peptide growth factor of MW 97-kDa, is a potent mitogen for parenchymal hepatocytes. HGF is present in normal serum and increases following liver injury or partial hepatectomy. In addition to liver, HGF mRNA has been detected in kidney. In cultured rabbit proximal tubule cells, recombinant human HGF (10 −10 M) increased DNA synthesis, measured as [ 3H] thymidine incorporation, from 1345±213 to 2931±636 cpm/10 6 cells; n=9; p<0.005). HGF was found to exert mitogenic effects at lower concentrations than epidermal growth factor (EGF), with half maximal effects seen at 6 × 10 −11 M compared to 7 ×10 −10 M for EGF. HGF was additive with EGF in stimulating [ 3H] thymidine incorporation. In addition to rabbit proximal tubule cells, HGF increased proliferation in a cultured mouse proximal tubule cell line, MCT, and in rat glomerular epithelial cells. In contrast, HGF did not stimulate proliferation of either rat mesangial cells or a rat aortic smooth muscle cell line, A 7r5. The HGF receptor is the product of the c-met proto-oncogene. C-met mRNA was detected in total kidney and in cultured proximal tubule cells but was not detected in cultured mesangial cells. In contrast, HGF mRNA was detected in mesangial cells but not in cultured proximal tubule cells. Preincubation of rabbit proximal tubule cells with the tyrosine kinase inhibitor, genistein (50 μM), prevented HGF-stimulation of [ 3H] thymidine incorporation. In LiCl pretreated rabbit proximal tubule cells loaded with [ 3H] myoinositol, HGF increased total inositol phosphate release, measured by anion exchange chromatography (control: 2181±414 vs HGF: 2609±478 cpm/1 6 cells; n=6; p<0.05). Although genistein did not affect baseline phosphoinositide hydrolysis, it inhibited the HGF stimulation. Thus, HGF is mitogenic for cultured proximal tubule cells as well as glomerular epithelial cells. Inhibition of proliferation and PI turnover by genistein suggests that HGF's actions are mediated in part by tyrosine kinase activity. In mammalian kidney, HGF relesased from mesangial cells may serve as a paracrine activator of the adjacent epithelial cells.

Adjustment to Pj depletion of the Na+—Pi co-transporter of mouse proximal tubule cells

Conference Article| June 01 1988 Adjustment to Pj depletion of the Na+—Pi co-transporter of mouse proximal tubule cells PETER J. BUTTERWORTH; PETER J. BUTTERWORTH 1Department of Biochemistry, King's College (London), Kensington Campus, Campden Hill Road, London W8 7AH, U.K. Search for other works by this author on: This Site PubMed Google Scholar MUSSARAT JAHAN MUSSARAT JAHAN 1Department of Biochemistry, King's College (London), Kensington Campus, Campden Hill Road, London W8 7AH, U.K. Search for other works by this author on: This Site PubMed Google Scholar Biochem Soc Trans (1988) 16 (3): 353. https://doi.org/10.1042/bst0160353 Article history Received: November 13 1987 Views Icon Views Article contents Figures & tables Video Audio Supplementary Data Peer Review Share Icon Share Facebook Twitter LinkedIn MailTo Cite Icon Cite Get Permissions Citation PETER J. BUTTERWORTH, MUSSARAT JAHAN; Adjustment to Pj depletion of the Na+—Pi co-transporter of mouse proximal tubule cells. Biochem Soc Trans 1 June 1988; 16 (3): 353. doi: https://doi.org/10.1042/bst0160353 Download citation file: Ris (Zotero) Reference Manager EasyBib Bookends Mendeley Papers EndNote RefWorks BibTex toolbar search Search Dropdown Menu toolbar search search input Search input auto suggest filter your search All ContentAll JournalsBiochemical Society Transactions Search Advanced Search This content is only available as a PDF. © 1988 Biochemical Society1988 Article PDF first page preview Close Modal You do not currently have access to this content.