Although converting an intersection into a roundabout has been shown to result in fewer injury accidents for both motor vehicle drivers and pedestrians, the effect on bicyclists’ safety is unclear or even negative. This study focuses on roundabouts without bicycle facilities (i.e., mixed traffic conditions) and makes use of semi-automated video observation software with the aim of analysing bicyclists’ behaviour and safety on roundabouts with different diameter. Four urban roundabouts in Belgium are observed. Interactions between bicyclists and other vehicles are analysed using speed, lateral position and five indicators to describe the closeness of interactions (TTCmin, PET, T2 min, lateral overtaking proximity and minimum distance headway). Additionally, the lateral position and riding speed of bicyclists that are in interaction with other vehicles is compared with the behaviour of bicyclists that are not in interaction with other vehicles.The behavioural analysis revealed that regardless of the type of condition (free-flow bicyclists or different interactions bicyclist-car), bicyclists always ride faster on roundabouts with big diameter and slower on roundabouts with small diameter. Moreover, bicyclists ride closer to the central island on roundabouts with big diameter compared to roundabouts with small diameter for all the conditions analysed.The analysis of surrogate safety indicators (TTCmin, PET, T2 min) revealed that close interactions between bicyclists and cars are relatively frequent at both small and big roundabouts. The percentages of close interactions are more or less equal for roundabouts with big diameter (7.86% of observed interactions) and roundabouts with small diameter (8.24%). The analysis of the indicators to describe the closeness of interactions also showed that the closest interactions at roundabouts are all situations where the bicyclist has a leading role. The analysis of the most common types of close interactions revealed indeed that the most common close interactions are interactions where the bicyclist is entering the roundabout. The analysis of lateral overtaking proximity showed that bicyclists who overtake a car take smaller lateral overtaking proximities compared to cars overtaking a bicyclist. The analysis of minimum distance headway finally revealed that bicyclists who ride behind a car take smaller distance headways compared to cars driving behind a bicyclist.