ABSTRACT Objective To examine the validity of the Motor Functional Development Scale for Young Children (DF-mot) to predict motor developmental delays in preterm infants. Method This retrospective cohort study includes 67 preterm infants who were assessed at 3–5 months by the DF-mot and the Alberta Infant Motor Scale (AIMS); and at 22–25 months by the Bayley Scales of Infant-Toddler Development (Bayley-III). The properties of the DF-mot and the AIMS were examined based on their ability to predict motor delays on the Bayley-III. Results The DF-mot gross motor subscale −2 SD and the AIMS 10th centile showed best balance between sensitivity and specificity (respectively Se = 57.1%, Sp = 71.7% and Se = 50%, Sp = 73.5%). Overall, the DF-mot fine motor subscale fails to predict motor delays. Conclusion The DF-mot shows a lack of sensitivity and of positive predictive value to accurately predict motor outcome at 2 years in preterm infants. Abbreviations CA, Corrected age; AIMS, Alberta Infant Motor Scale; DF-mot/PML, Motor Functional Development Scale for Young Children postural motor locomotor; DF-mot/EHGC, Motor Functional Development Scale for Young Children eye-hand grip coordination; Bayley-III/GM, Gross motor subscale of the Bayley Scales of Infant-Toddler Development Third Edition; Bayley-III/FM, Fine motor subscale of the Bayley Scales of Infant-Toddler Development Third Edition.