Neuromuscular Research Articles

A Narrative Review on Magnesium Sulfate as a Game Changer in Reducing ICU Stays in Organophosphate Poisoning Cases.

Organophosphate (OP) poisoning is a critical public health issue, particularly in agricultural regions where these compounds are extensively used as pesticides. The toxic effects of OP compounds arise from their inhibition of acetylcholinesterase, leading to an accumulation of acetylcholine and a subsequent cholinergic crisis, which can be fatal if not promptly treated. Traditional management of OP poisoning includes the administration of atropine and pralidoxime; however, these treatments often fall short of reducing the high morbidity and mortality associated with severe cases. Recent research has highlighted the potential of magnesium sulfate as an adjunctive treatment for OP poisoning. Magnesium sulfate exerts its beneficial effects through mechanisms such as calcium channel blockade and stabilization of neuromuscular junctions, which help mitigate the cholinergic hyperactivity induced by OP compounds. Clinical studies have shown that magnesium sulfate can significantly reduce the duration of intensive care unit (ICU) stays and improve overall patient outcomes. This narrative review aims to comprehensively analyze current insights into using magnesium sulfate to manage OP poisoning. It discusses the pathophysiology of OP poisoning, the pharmacological action of magnesium sulfate, and the clinical evidence supporting its use. Furthermore, the review will address the safety profile of magnesium sulfate and its potential role in current treatment guidelines. By synthesizing available evidence, this review seeks to establish magnesium sulfate as a game-changer in the management of OP poisoning, ultimately contributing to better clinical practices and patient outcomes.

Augmentation of functional recovery via ROCK/PI3K/AKT pathway by Fasudil Hydrochloride in a rat sciatic nerve transection model

BackgroundsThe functional recovery after peripheral nerve injury remains unsatisfactory. This study aims to perform a comprehensive evaluation of the efficacy of Fasudil Hydrochloride at treating the sciatic nerve transection injury in rats and the mechanism involved. Materials and methodsIn animal experiments, 75 Sprague Dawley rats that underwent transection and repair of the right sciatic nerve were divided into a control, Fasudil, and Fas + LY group, receiving daily intraperitoneal injection of saline, Fasudil Hydrochloride (10 mg/kg), and Fasudil Hydrochloride plus LY294002 (5 mg/kg), respectively. At day 3 after surgery, the expression of ROCK2, p-PI3K, and p-AKT in L4-5 DRG and the lumbosacral enlargement was determined using Western blotting. At day 7 and 14, axon density in the distal stump was evaluated with immunostaining using the anti-Neurofilament-200 antibody. At day 30, retrograde tracing by injecting Fluoro-gold in the distal stump was performed. Three months after surgery, remyelination was analyzed with immostaining using the anti-MPZ antibody and the transmission electron microscope; Moreover, Motion-Evoked Potential, and recovery of sensorimotor functions was evaluated with a neuromonitor, Footprint, Hot Plate and Von Frey Filaments, respectively. Moveover, the Gastrocnemius muscles were weighed, and then underwent H＆E staining, and staining of the neuromuscular junction using α-Bungarotoxin to evaluate the extent of atrophy and degeneration of the endplates in the Gastrocnemius. In vitro, spinal motor neurons (SMNs) and dorsal root ganglia (DRG) were cultured to examine the impact of Fasudil Hydrochloride and LY294002 on the axon outgrowth. ResultsThree days after injury, the expression of ROCK2 increased significantly (P＜0.01), and Fasudil application significantly increased the expression of p-PI3K and p-AKT in L4-6 DRG and the lumbosacral enlargement (P < 0.05). At day 7 and 14 after surgery, a higher axon density could be observed in the Fasudil group(P < 0.05). At day 30 after surgery, a larger number of motor and sensory neurons absorbing Fluoro-gold could be observed in the Fasudil group (P < 0.01) Three months after surgery, a greater thickness of myelin sheath could be observed in the Fasudil group (P < 0.05). The electrophysiological test showed that a larger amplitude of motion-evoked potential could be triggered in the Fasudil group (P < 0.01). Behavioral tests showed that a higher sciatic function index and a lower threshold for reacting to heat and mechanical stimuli could be measured in the Fasudil group. (P < 0.01). The wet weight ratio of the Gastrocnemius muscles and the area of the cross section of its myofibrils were greater in the Fasudil group (P < 0.01), which also demonstrated a higer ratio of axon-endplate connection and a larger size of endplates (P < 0.05). And there were no significant differences for the abovementioned parameters between the control and Fas + LY groups (P＞0.05). In vitro studies showed that Fasudil could significantly promote axon growth in DRG and SMNs, and increase the expression of p-PI3K and p-AKT, which could be abolished by LY294002 (P < 0.05). ConclusionsFasudil can augment axon regeneration and remyelination, and functional recovery after sciatic nerve injury by activating the PI3K/AKT pathway. The translational potential of this articleThe translation potential of this article is that we report for the first time that Fasudil Hydrochloride has a remarkable efficacy at improving axon regeneration and remyelination following a transection injury of the right sciatic nerve in rats through the ROCK/PI3K/AKT pathway, which has a translational potential to be used clinically to treat peripheral nerve injury.

Ocular versus generalized myasthenia gravis: a continuum associated with acetylcholine receptor antibody titers

The aim of this study was to evaluate clinical and serological differences between the ocular myasthenia gravis (oMG) and generalized MG (gMG). This study is a retrospective chart review, in which data was collected from patients fulfilling 2 of 3 diagnostic MG criteria (positive antibodies, evidence of neuromuscular transmission defect on neurophysiological examination, positive effect of pyridostigmine treatment). 350 patients were included and data concerning demographics and MG medical history were collected. Patients with oMG accounted for 15.7 % of the included patients. The two subgroups differed significantly in oMG having a later age at onset, lower AChR antibody-titers, longer doctor-to-diagnosis delay and less intensive MG treatment. Additionally, patients with oMG were faster at reaching a well-controlled disease state. Thymus pathology, number of antibody-positive (95.9 % of gMG and 94.5 % of oMG), sex, number of other autoimmune diseases and delay before drug stability did not differ between oMG and gMG. In conclusion, oMG is presumably a milder form of gMG characterized by lower AChR antibody-titers, a milder phenotype, and a quicker response to a less aggressive treatment. But otherwise, oMG and gMG show very similar characteristics, including the same frequency of positive AChR antibodies, which seems new compared to previous reports.

Mechanical Basis of Lumbar Intervertebral Disk Degeneration

Abstract The etiology of degenerative disk disease (DDD) is multifactorial. Among the various factors, mechanical processes contributing to endplate or discal injuries have been discussed as the initiating events in the degenerative cascade. DDD encompasses the multitudinous changes undergone by the different structures of the spinal segment, namely intervertebral disk (IVD), facet joints, vertebral end plate (VEP), adjoining marrow (Modic changes), and vertebral body. It has been etiologically linked to a complex interplay of diverse mechanisms. Mechanically, two different mechanisms have been proposed for intervertebral disk degeneration (IVDD): endplate-driven, especially in upper lumbar levels, and annulus-driven degeneration. VEP is the weakest link of the lumbar spine, and fatigue damage can be inflicted upon them under physiological loads, leading to the initiation of DDD. Disk calcification has been put forth as another initiator of inflammation, stiffening, and abnormal stresses across the IVD. The initial mechanical disruption leads to secondary IVDD through unfavorable loading of the nucleus pulposus and annulus fibrosis. The final degenerative cascade is then propagated through a combination of biological, inflammatory, autoimmune, or metabolic pathways (impaired transport of metabolites or nutrients). Abnormal spinopelvic alignment, especially pelvic incidence, also significantly impacts the degenerative process. Hence, the etiology of DDD is multifactorial. Mechanical pathways, including VEP injuries, increased disk stiffness, and abnormal spinopelvic alignment, play a significant role in the initiation of IVDD.

Simplified Formula for Nominal Force at Critical Welds in the Lower Chord Node of a Novel Bracket-Crane Truss Structure

In the realm of practical engineering, engineers commonly employ rod system models for modeling and analysis, which consequently precludes them from calculating the nominal forces exerted on welds at intricate nodes. This paper addresses the design challenges of the innovative bracket-crane truss structure by proposing a simplified nominal force calculation formula for critical welds of the integrated node. This study commences with the establishment of the frame model and ABAQUS multiscale models, utilizing engineering project drawings and data, followed by a verification of the similarities between the two simulation methods. This similarity of outcomes provides a foundation for directly using the computational results from the frame model in future calculations of the forces at the weld locations. From a mechanical standpoint, this paper derives nominal force calculation formulas for horizontal and vertical welds at critical locations for three node types. Additionally, a formula for calculating nominal shear forces in vertical welds at the end plate of support nodes is introduced. The applicability of these derived formulas is subsequently validated, ensuring their efficacy in accurately capturing relevant forces at critical locations. The presented nominal force calculation formula serves as a valuable tool for optimizing the design and guaranteeing the structural integrity of the integrated node within this distinctive engineering context.

Preliminary Study of Motion Preservation Following Posterior Dynamic Distraction Device in Adolescent Idiopathic Scoliosis Patients.

Motion-sparing scoliosis surgeries such as the posterior dynamic distraction device (PDDD) are slowly increasing in use. However, there is limited clinical data documenting postoperative motion across the PDDD construct. With this cohort study, we aim to measure sagittal and coronal motion following PDDD. We hypothesize coronal and sagittal spinal motion will be partially preserved across the construct. Retrospective review of prospectively collected data. Preoperative and minimum 1-year postoperative coronal range of motion across the instrumented levels was compared. Available flexion/extension radiographs were evaluated postoperatively to assess sagittal arc of motion. Radiographs from latest follow-up were used. At a mean of 1.9 years (1 to 5y), flexibility radiographs were available on 29 patients treated with PDDD (17 thoracic, 12 lumbar). Mean age at surgery was 16 years (12 to 25). Postoperative coronal arc of motion in PDDD patients was 11 degrees (3 to 19 degrees) in the thoracic spine and 10 degrees (0 to 28 degrees) in the lumbar spine. Compared with preoperative motion, the thoracic arc of motion was maintained by 33% (35 to 11 degrees) and lumbar motion was maintained by 30% (34 to 10 degrees). Flexion-extension radiographs were available on 7 patients. Sagittal arc for the upper instrumented vertebral end plate to the lower instrumented vertebral endplate of the cohort was 10 degrees in the thoracic spine (6 to 18) and 14 degrees in the lumbar spine (5 to 21). Sagittal measurements for the changes in the arc of the upper and lower screws on the construct were 4 degrees in the thoracic group (2 to 8) and 9 degrees in the lumbar group (2 to 17). By latest follow-up 11 patients (38%) underwent reoperation, with most cases due to implant breakage (N=4, 14%), extender misalignment (N=2, 7%), and screw misplacement (N=2, 7%). At mean 1.9 years postoperatively, PDDD preserves measurable spinal motion over the construct both in the coronal and the sagittal plane without evidence for autofusion. Coronal arc of motion averages 10 to 12 degrees and sagittal arc of motion ranged from 4 to 14 degrees, although this varies by patient. This study confirms that PDDD for pediatric scoliosis preserves a measurable degree of postoperative flexibility both in the sagittal and coronal planes. Level II-therapeutic study.

Hybrid isogeometric-based analysis and experimental investigation on the dynamic response characteristics of a clamped circular plate partially in contact with fluid

The aim of this study was to analyze the effect of fluid presence on the dynamic response characteristics of the thin circular plate and the distortions associated with the wet mode shapes. To this end, series of numerical calculations and experimental measurements were performed to investigate the free vibration characteristics of clamped circular plate under in-vacuo conditions and when it is partially in contact with fluid. In the experimental studies, the clamped boundary conditions are imposed on the circular end plate of the horizontal rigid cylindrical tank by closely-spaced bolts, and measurements were performed based on roving hammer impact technique. The proposed numerical approach was divided into two parts based on linear hydroelasticity theory. In the first stage, the thin circular plate is considered to be under in-vacuo conditions, and the mathematical model for this problem is developed based on the isogeometric finite element method (IGAFEM). The fluid environment is introduced in the second stage of the study in which the generalized in-vacuo modal displacements constitute the boundary conditions of the potential flow problem. The influence of fluid medium is incorporated in the system of equations in the form of fluid added mass, and the corresponding fluid forces are calculated by the isogeometric boundary element method (IGABEM). It is observed that the fluid presence has significant effects on the dynamic response characteristics of the test structure, and the specifically, the distortions of wet mode shapes were noticeable due to presence of free surface of the water. Overall, it is found that the natural frequencies and corresponding mode shapes obtained by conducted experiments and adapted numerical framework are in favorable agreement.

Effects of combined anodal transcranial direct current stimulation and motor control exercise on cortical topography and muscle activation in individuals with chronic low back pain: A randomized controlled study.

Aberrant movement in chronic low back pain (CLBP) is associated with a deficit in the lumbar multifidus (LM) and changes in cortical topography. Anodal transcranial direct current stimulation (a-tDCS) can be used to enhance cortical excitability by priming the neuromuscular system for motor control exercise (MCE), thereby enhancing LM activation and movement control. This study aimed to determine the effects of a 6-week MCE program combined with a-tDCS on cortical topography, LM activation, movement patterns, and clinical outcomes in individuals with CLBP. Twenty-two individuals with CLBP were randomly allocated to the a-tDCS group (a-tDCS; n=12) or sham-tDCS group (s-tDCS; n=10). Both groups received 20min of tDCS followed by 30min of MCE. The LM and erector spinae (ES) cortical topography, LM activation, movement control battery tests, and clinical outcomes (disability and quality of life) were measured pre- and post-intervention. Significant interaction (group×time; p<0.01) was found in the distance between LM and ES cortical locations. The a-tDCS group demonstrated significantly fewer discrete peaks (p<0.05) in both ES and LM and significant improvements (p<0.05) in clinical outcomes post-intervention. The s-tDCS group demonstrated a significant increase (p<0.05) in the number of discrete peaks in the LM cortical topography. No significant changes (p>0.05) in LM activation were observed in either group; however, both groups demonstrated improved movement patterns. Our findings suggest that combined a-tDCS with MCE can separate LM and ES locations over time while s-tDCS (MCE alone) reduces the distance. Our study did not find superior benefits of adding a-tDCS before MCE for LM activation, movement patterns, or clinical outcomes.

A Study of the Effects of Motor Experience on Neuromuscular Control Strategies During Sprint Starts.

Much of the current research on sprint start has attempted to analyze the biomechanical characteristics of elite athletes to provide guidance on the training of sprint technique, with less attention paid to the effects of motor experience gained from long-term training on neuromuscular control characteristics. The present study attempted to investigate the effect of motor experience on the modular organization of the neuromuscular system during starting, based on he clarification of the characteristics of muscle synergies during starting. It was found that exercise experience did not promote an increase in the number of synergies but rather a more focused timing of the activation of each synergy, allowing athletes to quickly complete the postural transition from crouching to running during the starting.

Experimental and three-dimensional performance analysis of a bio-inspired fish-ridge vertical axis wind rotor

The savonius vertical axis wind rotor has a simple structure, making it a suitable choice for power generation in small-scale, low wind speed environments. However, its wind energy conversion efficiency is low. To improve the conversion efficiency of the wind rotor using the proposed fish-bridge structure, the wind tunnel tests are conducted to collect actual wind and rotational speeds. In addition, Computational Fluid Dynamics (CFD) simulation is conducted to reveal potential operating mechanisms, with special emphasis on analyzing the effects of aspect ratio and end plate on the performance of the fish-ridge wind rotor. The CFD results show that the wind power coefficient of this wind rotor presents an increasing trend with the increase of the aspect ratio and the area of the end plate, and that with round end plates is improved by 24.26 % compared to that of the wind rotor without end plates, and that with an aspect ratio of 3.5 is improved by 32.58 % compared to that of the wind rotor with an aspect ratio of 1. By increasing the aspect ratio and the end plate area of the wind rotor, the contact airflow is raised and concentrated, which improves the aerodynamic performance of the wind rotor.

Finite Element Analysis of Seismic Performance of RCS Hybrid Joints of Steel Hoop Structure

Reinforced concrete column-steel beam (RCS) composite structures are widely used in super highrise buildings due to their excellent performance. In this paper, the numerical simulation analysis of the RCS hybrid joints of “beam through” and “column through” RCS constructed with steel hoop structure are carried out, and the influence of five different parameters, i. e. , flange width-thickness ratio, steel yield strength, axial compression ratio, concrete strength and end plate thickness, on the seismic performance and failure mode of RCS hybrid joints is investigated. The results show that the ABAQUS finite element model can simulate the performance of RCS mixing under static load through reasonable parameter settings, which is in good agreement with the experimental results. Under the mechanism of “strong column and weak beam”, the combined node firstly undergoes beam-hinge damage, and the change of the axial compression ratio and the concrete strength parameter of the joint have little effect on the ultimate bearing capacity and deformation performance of the joint. Increasing the flange width-thickness ratio reduces the bearing capacity of the joint by up to 11%, and increases the yield strength of the steel, and increases the bearing capacity of the joint by 36. 4%. The thickness of the end plate of the column through specimen is increased, and the bearing capacity and deformation performance of the joint are improved to a certain extent. The changes of each parameter have no significant effect on the final plastic hinge failure mode of the joint. The research in this paper provides theoretical support for the design and research of this type of node in the future.

Identification of adrenergic presynaptic and postsynaptic protein locations at neuromuscular junctions, their decrease during aging, and recovery by nicotinamide mononucleotide administration.

Neuromuscular junctions are innervated by motor and sympathetic nerves. The sympathetic modulation of motor innervation shows functional decline during aging, but the cellular and molecular mechanism of this change is not fully known. This study aimed to evaluate the effect of aging on sympathetic nerves and synaptic proteins at mouse neuromuscular junctions. Sympathetic nerves, presynaptic, and postsynaptic proteins of sympathetic nerves at neuromuscular junctions were visualized using immunohistochemistry, and aging-related changes were compared between adult-, aged-, and nicotinamide mononucleotide (NMN) administered aged mice. Sympathetic nerves were detected by anti-tyrosine hydroxylase antibody, and presynaptic protein vesicular monoamine transporter 2 colocalized with the sympathetic nerves. These two signals surrounded motor nerve terminals and acetylcholine receptor clusters. Postsynaptic neurotransmitter receptor β2-adrenergic receptors colocalized with motor nerve terminals and resided in reduced density at extrasynaptic sarcolemma. The signal intensity of the sympathetic nerve marker did not show a significant difference at neuromuscular junctions between 8.5-month-old adult mice and 25-month-old aged mice. However, the signal intensity of vesicular monoamine transporter 2 and β2-adrenergic receptors showed age-related decline at neuromuscular junctions. Interestingly, both age-related declines reverted to the adult level after 1 month of oral administration of NMN by drinking water. In contrast, NMN administration did not alter the expression level of sympathetic marker tyrosine hydroxylase at neuromuscular junctions. The results suggest a functional decline of sympathetic nerves at aged neuromuscular junctions due to decreases in presynaptic and postsynaptic proteins, which can be reverted to the adult level by NMN administration.

A Case Report and Literature Review of New-Onset Myasthenia Gravis after COVID-19 Infection

Myasthenia gravis (MG) is an autoimmune disorder affecting the neuromuscular junction caused by a B-cell-mediated, T-cell-dependent immunologic attack at the end plate of the postsynaptic membrane. Attack on muscle acetylcholine receptors (AChR) of the postsynaptic membrane due to the AChR, muscle-specific tyrosine kinase, or lipoprotein receptor-related peptide 4 antibodies lead to symptoms of painless, fluctuating weakness of muscle groups and often begins with ocular signs and symptoms. Coronavirus disease 2019 (COVID-19) is an acute respiratory disease caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), a novel coronavirus closely related to SARS-CoV. Serious neurologic complications are infrequent and diverse with reported cases of stroke, encephalitis/meningitis, Guillain-Barré syndrome, acute disseminated encephalomyelitis, ataxia, and unspecified limb weakness. MG is a rarely reported sequela of COVID-19 infection. To date, there are 15 reported cases of post-COVID-19 MG. In this article, we present a case of post-COVID-19 MG and a concise review of other reported cases. An 83-year-old Caucasian male with a medical history of atrial fibrillation status post-ablation and non-ischemic cardiomyopathy was initially admitted for COVID-19 pneumonia. He was treated with remdesivir, convalescent plasma, and supplemental oxygen therapy but did not require invasive mechanical intubation. One month after discharge, he started experiencing fatigue with muscle weakness and progressive dyspnea. He progressed to develop dysphonia, especially at the end of the day. After extensive workup, he was diagnosed with MG with a positive antibody against the AChR. The chronological events of developing slowly worsening muscular weakness after recovering from COVID-19 infection and positive AChR antibody led to the diagnosis of post-COVID-19 new-onset MG. Post-COVID-19 fatigue, long-term use of steroids, and intensive care unit-related physical deconditioning ....

Comparative electrophysiological characterization of ammodytoxin A, a β-neurotoxin from the nose-horned viper venom, and its enzymatically inactive mutant

Presynaptic- or β-neurotoxicity of secreted phospholipases A2 (sPLA2) is a complex process. For full expression of β-neurotoxicity, the enzymatic activity of the toxin is essential. However, it has been shown that not all toxic effects of a β-neurotoxin depend on its enzymatic activity, for example, the inhibition of mitochondrial cytochrome c oxidase. The main objective of this study was to verify whether it is possible to observe and study the phospholipase-independent actions of β-neurotoxins by a standard ex vivo twitch-tension experimental approach. To this end, we compared the effects of a potent snake venom β-neurotoxin, ammodytoxin A (AtxA), and its enzymatically inactive mutant AtxA(D49S) on muscle contraction of the mouse phrenic nerve-hemidiaphragm preparation. While AtxA significantly affected the amplitude of the indirectly evoked isometric muscle contraction, the resting tension of the neuromuscular (NM) preparation, the amplitude of the end-plate potential (EPP), the EPP half decay time and the resting membrane potential, AtxA(D49S) without enzymatic activity did not. From this, we can conclude that the effects of AtxA independent of enzymatic activity cannot be studied with classical electrophysiological measurements on the isolated NM preparation. Our results also suggest that the inhibition of cytochrome c oxidase activity by AtxA is not involved in the rapid NM blockade by this β-neurotoxin, but that its pathological consequences are rather long-term. Interestingly, in our experimental setup, AtxA upon direct stimulation reduced the amplitude of muscle contraction and induced contracture of the hemidiaphragm, effects that could be interpreted as myotoxic.

Gap-junction-mediated bioelectric signaling required for slow muscle development and function in zebrafish

Bioelectric signaling, intercellular communication facilitated by membrane potential and electrochemical coupling, is emerging as a key regulator of animal development. Gap junction (GJ) channels can mediate bioelectric signaling by creating a fast, direct pathway between cells for the movement of ions and other small molecules. In vertebrates, GJ channels are formed by a highly conserved transmembrane protein family called the connexins. The connexin gene family is large and complex, creating challenges in identifying specific connexins that create channels within developing and mature tissues. Using the embryonic zebrafish neuromuscular system as a model, we identify a connexin conserved across vertebrate lineages, gjd4, which encodes the Cx46.8 protein, that mediates bioelectric signaling required for slow muscle development and function. Through mutant analysis and invivo imaging, we show that gjd4/Cx46.8 creates GJ channels specifically in developing slow muscle cells. Using genetics, pharmacology, and calcium imaging, we find that spinal-cord-generated neural activity is transmitted to developing slow muscle cells, and synchronized activity spreads via gjd4/Cx46.8 GJ channels. Finally, we show that bioelectrical signal propagation within the developing neuromuscular system is required for appropriate myofiber organization and that disruption leads to defects in behavior. Our work reveals a molecular basis for GJ communication among developing muscle cells and reveals how perturbations to bioelectric signaling in the neuromuscular system may contribute to developmental myopathies. Moreover, this work underscores a critical motif of signal propagation between organ systems and highlights the pivotal role of GJ communication in coordinating bioelectric signaling during development.

Spinal motor neuron development and metabolism are transcriptionally regulated by Nuclear Factor IA.

Neural circuits governing all motor behaviors in vertebrates rely on the proper development of motor neurons and their precise targeting of limb muscles. Transcription factors are essential for motor neuron development, regulating their specification, migration, and axonal targeting. While transcriptional regulation of the early stages of motor neuron specification is well-established, much less is known about the role of transcription factors in the later stages of maturation and terminal arborization. Defining the molecular mechanisms of these later stages is critical for elucidating how motor circuits are constructed. Here, we demonstrate that the transcription factor Nuclear Factor-IA (NFIA) is required for motor neuron positioning, axonal branching, and neuromuscular junction formation. Moreover, we find that NFIA is required for proper mitochondrial function and ATP production, providing a new and important link between transcription factors and metabolism during motor neuron development. Together, these findings underscore the critical role of NFIA in instructing the assembly of spinal circuits for movement.

Intelligent Soft Quasi‐Organism Equipped with Sensor‐Driven Integrated Tentacles

AbstractIntegration of soft electronic system components is critical to create a closed‐loop system that seamlessly integrates sensing, driving, processing, and autonomous control capabilities. However, the existence of these components, particularly sensors and actuators, in isolated or discrete forms complicates the endeavor to achieve seamless interface matching and in situ integration, no more than the autonomic system. Here, an intelligent soft quasi‐organism (SQO) equipped with sensor‐driven integrated tentacles is demonstrated, featuring real‐time state recognition and autonomous object recognition inspired by the sea anemones. By employing a heterogeneous mechanisms homotopic integration (HMHI) strategy, the tentacles of the SQO possess the unique ability to simultaneously perceive state changes and flexibly drive motions, utilizing the same material and structure. The sea anemone‐shaped SQO exhibits real‐time autonomous state identification capabilities through the integration of machine learning and customized circuits, attaining 100% recognition accuracy across sixteen states. With a neuromuscular system that facilitates active autonomous perception, the anemone‐shaped SQO can recognize and intelligently grasp static objects with an accuracy of 80.7%, surpassing that of a human hand (74.7%). The SQO provides a promising approach for realizing artificial neuromuscular systems, with great potential for applications in crucial areas such as intelligent soft robotics and in vivo therapy.

Myo-optogenetics: optogenetic stimulation and electrical recording in skeletal muscles.

Complex movements involve highly coordinated control of local muscle elements. Highly controlled perturbations of motor outputs can reveal insights into the neural control of movements. Here we introduce an optogenetic method, compatible with electromyography (EMG) recordings, to perturb muscles in transgenic mice. By expressing channelrhodopsin in muscle fibers, we achieved noninvasive, focal activation of orofacial muscles, enabling detailed examination of the mechanical properties of optogenetically evoked jaw muscle contractions. We demonstrated simultaneous EMG recording and optical stimulation, revealing the electrophysiological characteristics of optogenetically triggered muscle activity. Additionally, we applied optogenetic activation of muscles in physiologically and behaviorally relevant settings, mapping precise muscle actions and perturbing active behaviors. Our findings highlight the potential of muscle optogenetics to precisely manipulate muscle activity, offering a powerful tool for probing neuromuscular control systems and advancing our understanding of motor control.

Nesfatin-1 ameliorates pathological abnormalities in Drosophila hTau model of Alzheimer's disease

In human Alzheimer's disease (AD), the aggregation of tau protein is considered a significant hallmark, along with amyloid-beta. The formation of neurofibrillary tangles due to aberrant phosphorylation of tau disrupts microtubule stability, leading to neuronal toxicity, dysfunction, and subsequent cell death. Nesfatin-1 is a neuropeptide primarily known for regulating appetite and energy homeostasis. However, the function of Nesfatin-1 in a neuroprotective role has not been investigated. In this study, we aimed to elucidate the effect of Nesfatin-1 on tau pathology using the Drosophila model system. Our findings demonstrate that Nesfatin-1 effectively mitigates the pathological phenotypes observed in Drosophila human Tau overexpression models. Nesfatin-1 overexpression rescued the neurodegenerative phenotypes in the adult fly's eye and bristle. Additionally, Nesfatin-1 improved locomotive behavior, neuromuscular junction formation, and lifespan in the hTau AD model. Moreover, Nesfatin-1 controls tauopathy by reducing the protein level of hTau. Overall, this research highlights the potential therapeutic applications of Nesfatin-1 in ameliorating the pathological features associated with Alzheimer's disease.

An innovative electrolytic cation exchange reactor system for cleaner generation of hydrogen gas using ocean water

The study presents a novel integrated three-compartment electrochemical continuous electrodeionization reactor that was constructed in a laboratory environment. In order to extract large amounts of carbon dioxide from naturally occurring oceanwater in the form of bicarbonate and carbonate, it develops a innovative electrolytic cation exchange process. At the same time, it produces hydrogen gas for possible hydrocarbon synthesis. The study employs the Box-Behnken experimental design approach implemented through the Design Expert software to investigate and optimize the performance of electrochemical hydrogen extraction from Ocean water from an integrated reactor. The integrated electrochemical continuous-type electrodeionization reactor comprises two cation-permeable membranes that operate as boundaries between three compartments: a central compartment and electrode compartments that house the cathode and anode, each of which may reverse polarity. These membranes are made of gel polystyrene cross-linked with divinylbenzene, which has acidic properties that allow cation transport while inhibiting anions. The integrated reactor's electrode chambers are enclosed in plexiglass end plates and contain distinct Plexiglass electrode compartments. These compartments have mesh and solid steel anode and cathode electrodes, with an electrode active area of 176.71 cm2. The influence of various factors, such as voltage and electrolyte amount, on the production of hydrogen synthesis is examined. The study results demonstrate a maximum hydrogen production rate of 2.2 mg/min and a hydrogen production efficiency of 7.82%. The lab testing was carried out using experimental equipment to determine viability. The tests included 35-min runs that measured hydrogen generation under various parameters, such as applied voltage, electrolyte concentration, and pH levels. The ocean salt solutions of 0.5M, 1M, and 2M were initially prepared and evaluated for hydrogen generation. Each concentration is assessed at a voltage range of 4–15V, with matching solution pH values ranging from 2 to 7. Optimization studies were conducted to enhance the hydrogen production rate. The results indicate that hydrogen production rises proportionally with applied voltage and electrolyte concentration but falls with pH growth. Within the Design Expert software, the statistical methods for response surface analysis and optimization are utilized accordingly.