Motor Coordination Ability Research Articles

Heterozygous KCNJ10 Variants Affecting Kir4.1 Channel Cause Paroxysmal Kinesigenic Dyskinesia.

More than 60% of paroxysmal kinesigenic dyskinesia (PKD) cases are of uncertain variants. The aim was to elucidate novel genetic contribution to PKD. A total of 476 probands with uncertain genetic causes were enrolled for whole-exome sequencing. A method of case-control analysis was applied to identify the candidate genes. Whole-cell patch-clamp recording was applied to verify the electrophysiological impact of the identified variants. A mouse model with cerebellar heterozygous knockout of the candidate gene was developed via adeno-associated virus injection, and dystonia-like phenotype inducement and rotarod tests were performed. In vivo multiunit electrical recording was applied to investigate the change in neural excitability in knockout mice. Heterozygous variants of potassium inwardly rectifying channel subfamily J member 10 (KCNJ10) clustered in PKD patients were compared with those in the control groups. Fifteen variants were detected in 16 of 522 probands (frequency = 3.07%). Patients with KCNJ10 variants tended to have a milder manifestation compared to those with PRRT2 (proline-rich transmembrane protein 2) variants. KCNJ10 variants partially altered the transmembrane location of inwardly rectifying potassium channel 4.1 (Kir4.1). The Kcnj10 expression is consistent with the natural course of PKD. Variants resulted in different degrees of reduction in cell Kir4.1 currents, and mice with heterozygous conditional knockout of Kcnj10 in the cerebellum presented dystonic posture, together with poor motor coordination and motor learning ability in rotarod tests. The firing rate of deep cerebellar nuclei was significantly elevated in Kcnj10-cKO mice. We identified heterozygous variants of KCNJ10 in PKD. Impaired function of Kir4.1 might lead to abnormal neuronal excitability, which attributed to PKD. © 2024 International Parkinson and Movement Disorder Society.

The impact of dexmedetomidine on ketamine-induced neurotoxicity and cognitive impairment in young mice.

The potential neuroprotective effects of dexmedetomidine against ketamine-induced neurotoxicity remain inconclusive. This study aims to investigate the influence of dexmedetomidine on ketamine-induced neuronal apoptosis and neurodevelopmental toxicity. In vitro experiments employed concentrations of 0.1uM for dexmedetomidine and 50 uM for ketamine individually as well as their combination. Changes in apoptotic proteins and dendritic development in neurons were assessed after a 6-h exposure to the drugs with evaluations conducted 24hs' post-treatment. In vivo experiments entailed intraperitoneal administration starting from postnatal Day 7 (P7) continuously for 3days (P7-P9) using dosages of 100 mg/kg for ketamine and 1mg/kg for dexmedetomidine alone or combined. Learning, memory and motor coordination abilities were evaluated via rotary rod tests and shuttle box experiments at P30 and P60, respectively. Dexmedetomidine effectively mitigated ketamine-induced apoptosis in hippocampal neurons but did not alleviate associated dendritic developmental abnormalities. Although causing reduced motor coordination in mice, no improvement was observed with regard to this effect or reaction speed when treated with dexmedetomidine alongside ketamine. This study demonstrates that while dexmedetomidine can mitigate ketamine-induced neuronal apoptosis, it has limited impact on its associated neurodevelopmental toxicities.

A Survey Study on the Maturity of Schooling of Young Children in Rural Sichuan Province:Taking the Rural Area of Meishan City,Sichuan Province as An Example

The admission maturity level of preschoolers can predict their performance after entering schools to a certain extent. Therefore, how to prepare for children’s admission to primary school has become a key issue of social concern. In order to improve the admission maturity level of rural preschoolers in Sichuan Province, it is necessary to understand the admission maturity level of 5-6-year-old children in Sichuan Province, so as to provide theoretical and practical basis for taking targeted measures. In this study, the measurement method was used to investigate the admission maturity level of children in rural areas of Meishan City, Sichuan Province as the research sample. It was found that rural children's motor coordination ability did not differ significantly from the national average, and their scores in visual perception ability, auditory perception ability, motor coordination ability, perceptual conversion ability, mathematical preparation ability, verbal communication ability, social adaptation ability and learning quality were significantly lower than the national average, but in auditory perceptual ability, rural children's scores were significantly higher than the national average. The differences in eight abilities between males and females were compared. Except for motor coordination, perceptual conversion ability, and verbal communication ability, there were significant differences in all other aspects, and girls scored higher than boys in all abilities. In addition to this, there is also some discussion on mother's occupation and young children's maturity in school. Based on the findings, targeted improvement measures were proposed to provide a reference for home and school to improve children's admission to primary school.

The Role of Virtual Reality in Enhancing Motor Skills in Children: A Systematic Review

This research aims to analyze how virtual reality (VR) can enhance movement skills in children. We employed a systematic review model, searching for relevant articles in databases such as PubMed, Web of Science, and ScienceDirect. The search keywords included "virtual reality," "child," "social," and "motor skills." This study's inclusion criteria were articles focused on "virtual reality," "children with motor disorders," and "motor skills," published within the last five years (as of 2019). Articles from less well-known journals were excluded. We identified eight articles that met the inclusion criteria and were suitable for systematic review according to the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) standards. The findings indicate that VR plays a significant role in improving motor coordination abilities in children. Responses to VR interventions were overwhelmingly positive, showing reductions in physical, cognitive, and emotional stress. Therefore, VR proves to be a valuable tool for physiotherapists and physicians treating children with motor delays. However, some considerations need to be addressed. A few parents have raised concerns about the high costs and the substantial space requirements associated with VR setups. Keywords: virtual reality, motion disorders, motor, technology, children

NEAT1 Promotes Valproic Acid-Induced Autism Spectrum Disorder by Recruiting YY1 to Regulate UBE3A Transcription.

Evidence suggests that long non-coding RNAs (lncRNAs) play a significant role in autism. Herein, we explored the functional role and possible molecular mechanisms of NEAT1 in valproic acid (VPA)-induced autism spectrum disorder (ASD). A VPA-induced ASD rat model was constructed, and a series of behavioral tests were performed to examine motor coordination and learning-memory abilities. qRT-PCR and western blot assays were used to evaluate target gene expression levels. Loss-and-gain-of-function assays were conducted to explore the functional role of NEAT1 in ASD development. Furthermore, a combination of mechanistic experiments and bioinformatic tools was used to assess the relationship and regulatory role of the NEAT1-YY1-UBE3A axis in ASD cellular processes. Results showed that VPA exposure induced autism-like developmental delays and behavioral abnormalities in the VPA-induced ASD rat model. We found that NEAT1 was elevated in rat hippocampal tissues after VPA exposure. NEAT1 promoted VPA-induced autism-like behaviors and mitigated apoptosis, oxidative stress, and inflammation in VPA-induced ASD rats. Notably, NEAT1 knockdown improved autism-related behaviors and ameliorated hippocampal neuronal damage. Mechanistically, it was observed that NEAT1 recruited the transcription factor YY1 to regulate UBE3A expression. Additionally, in vitro experiments further confirmed that NEAT1 knockdown mitigated hippocampal neuronal damage, oxidative stress, and inflammation through the YY1/UBE3A axis. In conclusion, our study demonstrates that NEAT1 is highly expressed in ASD, and its inhibition prominently suppresses hippocampal neuronal injury and oxidative stress through the YY1/UBE3A axis, thereby alleviating ASD development. This provides a new direction for ASD-targeted therapy.

Safranal exerts a neuroprotective effect on Parkinson's disease with suppression of NLRP3 inflammation activation.

Parkinson's disease (PD) is a common central nervous system neurodegenerative disease. Neuroinflammation is one of the significant neuropathological hallmarks. As a traditional Chinese medicine, Safranal exerts anti-inflammatory effects in various diseases, however, whether it plays a similar effect on PD is still unclear. The study was to investigate the effects and mechanism of Safranal on PD. The PD mouse model was established by 1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine MPTP firstly. Next, the degree of muscle stiffness, neuromuscular function, motor retardation and motor coordination ability were examined by observing and testing mouse movement behavior. Immunofluorescence staining was used to observe the expression of tyrosine hydroxylase (TH). The dopamine (DA) content of the striatum was detected by High-performance liquid chromatography (HPLC). The expression of TH and NLRP3 inflammasome-related markers NLRP3, IL-1β, and Capase-1 were detected by Real-time Polymerase Chain Reaction (qRT-PCR) and western blotting (WB) respectively. Through behavioral testing, Parkinson's mouse showed a higher muscle stiffness and neuromuscular tension, a more motor retardation and activity disorders, together with a worse motor coordination compared with sham group. Simultaneously, DA content and TH expression in the striatum were decreased. However, after using Safranal treatment, the above pathological symptoms of Parkinson's mouse all improved compared with Safranal untreated group, the DA content and TH expression were also increased to varying degrees. Surprisingly, it observed a suppression of NLRP3 inflammation in the striatum of Parkinson's mouse. Safranal played a neuroprotective effect on the Parkinson's disease and its mechanism was related to the inhibition of NLRP3 inflammasome activation.

Biological prerequisites of schoolchildren's movement culture

The paper is devoted to determining biological (anatomical and physiological) prerequisites for the formation of schoolchildren's movement culture. The motor activity of schoolchildren and their physical performance is a component of the concept of health and is determined by the anatomical and physiological features of the body, its anthropometric indicators, the state of the musculoskeletal system, the state of the cardiorespiratory system, the degree of maturity of the nervous system, and the mental state. Purpose: to substantiate the dependence of pupils' movement culture formation on anatomical and physiological features of pupils' organism. Research methods: theoretical - analysis and generalization of literary sources. It has been determined that motor culture is a component of the general culture of the personality, its mental activity and behavior and is unique as it can reveal the personal potential of the child. Movement culture is formed as a complex of components: motor (physical qualities and coordination abilities); aesthetic (expressiveness, artistry, plasticity, correct posture); personal (temperament, attention, memory). It is scientifically proven that the mental and physical health of schoolchildren depends on the formation of skills for a healthy physically active lifestyle, education of personal physical culture, culture of physical activity, sports culture with the obligatory consideration of age and individual characteristics of schoolchildren; properly organized physical education and sports, taking into account the individual anatomical and physiological characteristics of the child, should have a positive impact on the growing body of schoolchildren, promote harmonization of physical and mental development.

Analysis of motor coordination abilities of students: Comparative study of students in urban and rural areas

This study aims to analyze the differences in coordination skills of children who live in urban and rural areas. This study used a cross-sectional design, and the research subjects were elementary school students aged between 7 - 9 years (N=640; male=320 and female=320) spread across urban and rural areas in West Sumatra Province, Indonesia. Samples were collected through a purposive sampling technique. Coordination data were measured using the Körperkoordinations Test für Kinder (KTK): balance beam, moving sideways, jumping sideways and eye-hand coordination. The data were analyzed using the IBM SPSS statistical program version 25. The analysis results revealed that Urban students had a superior motor coordination average score compared to rural students. Based on the t-test, there was a significant difference in general coordination abilities between urban and rural students (p value< 0.05). The motor coordination skills of students in urban settings are superior to those of students in rural settings, both male and female. It is believed that by having adequate facilities and equipment, schools in Urban have more opportunities to practice coordination both structured and independently. It is recommended to optimize movement coordination training for students in a structured manner in physical education and extracurricular learning at school.

Rat Model of Closed-Head Mild Traumatic Injury and its Validation.

Animal models are crucial for advancing our understanding of mild traumatic brain injury (mTBI) and guiding clinical research. To achieve meaningful insights, developing a stable and reproducible animal model is essential. In this study, we report a detailed description of a closed-head mTBI model and a representative validation method using Sprague-Dawley rats to verify the modeling effect. The model involves dropping a 550 g mass weight from a height of 100 cm directly onto the head of a rat on a destructible surface, followed by a 180-degree turn. To assess the injury, rats underwent a series of neurobehavioral assessments 10 min post-injury, including time of loss of consciousness, first seeking-behavior time, escape ability, and beam balance ability test. During the acute and subacute stages following the injury, behavioral tests were conducted to assess motor coordination ability (Beam task), anxiety (Open Field test), and learning and memory abilities (Morris Water Maze test). The closed-head mTBI model produced a consistent injury response with minimal mortality and replicated real-life situations. The validation method effectively verified the model development and ensured the stability and consistency of the model.

The effect of rhythm training on the motor coordination abilities of 8-12-year-old freestyle swimmers.

This study aims to evaluate the effect of rhythm training in the early coordination development and to find out its role in the transfer of specific performance in young swimmers. Eighty young swimmers aged eight to twelve were divided randomly into a control group (CON, n=40, M/F=17/23) or an experimental group (EXP, n=40, M/F=19/21). A total of 72 swimmers completed all the training programs and tests included in this study (CON, n=35, M/F=15/20; EXP, n=37, M/F=16/21). The training programme lasted 12 weeks. During the study period, all groups participated in the same swimming training program with the control group participating six times a week, and the experimental group participating four times a week with an additional two rhythm trainings each week. The height, weight, walking backwards ability, jumping sideways ability, and 25 m freestyle were measured for each participant and an index of coordination was analyzed from recordings of the participant's 25 m freestyle. The results indicated that participation in rhythm training resulted in improved general motor and swimming-specific coordination among the swimmers, but the results varied by number of years of sport-specific training experience. The swimmers with more training experience improved more in swimming-specific coordination but less in general motor coordination. This study strengthens the evidence for the effectiveness of early rhythm training in swimmers, indicating that it is feasible to design programs to address general and sport-specific coordination in young athletes.

Motor Coordination in Children: A Comparison between Children Engaged in Multisport Activities and Swimming.

Motor coordination has a crucial role in various physical activities and sports, highlighting its significance in overall movement proficiency and performance. This study aimed to compare motor coordination in children engaged in multisport versus swimming activities. The participants of this study included 180 boys and girls (girls = 87) aged 8.25 years ± 0.89. A total of three groups were included: group 1 consisted of inactive children, group 2 included children participating in swimming, and group 3 included children enrolled in multisport. Motor coordination was assessed using the Kiphard-Schilling body coordination test, evaluated by motor quotient (MQ): walking backwards, hopping for height, jumping sideways, and moving sideways. Additionally, a total motor quotient (Total MQ) was calculated based on the performance in all four tests. ANOVA revealed a significant difference in Total MQ and all subtests between the groups (p < 0.01). A significant difference in Total MQ was found not only between the inactive and multisport groups (Diff = 19.8000; 95%CI = 13.1848 to 26.4152; p = 0.001) but also between the multisport and swimming groups (Diff = 12.8000; 95%CI = 6.3456 to 19.2544; p = 0.001). In conclusion, the results revealed that children involved in multisport activities exhibited significantly better motor coordination compared to both the swimming group and the inactive group. Therefore, to enhance the growth of motor coordination abilities, it is crucial that parents, instructors, and coaches encourage kids to engage in multisport physical activities on a daily basis.

Pattern of Visual-Motor Integration, Visual Perception, and Fine Motor Coordination Abilities in Children Being Assessed for Fetal Alcohol Spectrum Disorder.

Motor skill assessment is part of the fetal alcohol spectrum disorder (FASD) multidisciplinary assessment. Some clinicians opt to exclude assessment of the subcomponents of visual-motor integration (visual perception and motor coordination), on the assumption that challenges will be revealed based on the assessment of visual-motor integration. The objective is to describe the visual-motor integration, visual perception, and fine motor coordination pattern of abilities in children with confirmed prenatal alcohol exposure being assessed for fetal alcohol spectrum disorder. This cross-sectional study included 91 children (65 males; mean age: 10 years, 6 months SD = 2 years, 10 months) undergoing assessment for FASD. Friedman and Wilcoxon statistics were used to compare mean visual-motor integration, visual perception, and fine motor coordination percentiles from the Beery-Buktenica Developmental Test of Visual-Motor Integration, Sixth Edition (Beery-6). Children being assessed for FASD (n = 91) had the highest normative scores in visual perception, followed by visual-motor integration and fine motor coordination (mean percentiles (SD): 35.9 (24.9), 20.6 (18.3), and 13.8 (15.5), respectively) (χ 2 distribution = 46.909, p ≤ 0.001). Children being assessed for FASD experience more challenges with fine motor coordination compared with visual-motor integration and visual perception tasks. This pattern differs from the pattern established for the general population in which tasks that require visual-motor integration are more challenging than tasks that isolate visual perception and fine motor coordination. These results suggest that fine motor coordination should be included in FASD diagnostic assessments and considered as an area for intervention.

Methods of correction of psychomotor development of preschool children with autism spectrum disorders

The article is based on the study of scientific and methodological literature, pedagogical observations and own experience of working with autistic children, theoretical analysis and generalization of scientific and methodological approaches and methods of correction of psychomotor development of preschool children with autism spectrum disorders, which manifest themselves in early childhood and are considered one of the most severe disorders of psychophysical development of this category of children, are carried out.&#x0D; It is noted that the activities aimed at correcting psychomotor development are quite specific and diverse, have a certain evidence base and affect the process of socialization of autistic children. On this basis, the article reveals the relationship between the motor activity of autistic children and other types of activity - intellectual, speech and emotional; systematizes the means and methods of correction of motor activity and behavior of this contingent of children.&#x0D; The necessity and importance of solving the problems of correction and development of motor activity of autistic children, taking into account the peculiarities of their psychophysical development, are theoretically substantiated. Based on the experience of researchers on this issue, the approaches, means and methods that should be taken into account when developing correctional programs for this contingent of children are identified.&#x0D; It has been established that successful correction of psychomotor development and socialization of autistic children requires gradualness, perseverance, cooperation with parents, and the use of a comprehensive, correctional and developmental, sensory and perceptual, eclectic, behavioral, variable and individual approach to each autistic child. It has been found that the main means of correcting the psychomotor development of children with autism spectrum disorders include: outdoor and sensory games, imitation and gymnastic exercises, exercise therapy exercises aimed at developing gross and fine motor skills, coordination and strength abilities, as well as exercises for intercortical interaction, swimming and massage; the main methods of correcting psychomotor development are the method of early intervention, the method of imitation, the tandem method and the game method.

The ethanol extract of Scutellaria baicalensis Georgi attenuates complete Freund's adjuvant (CFA)-induced inflammatory pain by suppression of P2X3 receptor

Ethnopharmacological relevanceScutellaria baicalensis Georgi (SBG) is a perennial herb with anti-inflammatory, antibacterial, and antioxidant activities, which is traditionally used to treat inflammation of respiratory tract and gastrointestinal tract, abdominal cramps, bacterial and viral infections. Clinically, it is often used to treat inflammatory-related diseases. Research has shown that the ethanol extract of Scutellaria baicalensis Georgi (SGE) has anti-inflammatory effect, and its main components baicalin and baicalein have analgesic effects. However, the mechanism of SGE in relieving inflammatory pain has not been deeply studied. Aim of the studyThis study aimed to evaluate the analgesic effect of SGE on complete Freund's adjuvant (CFA)-induced inflammatory pain rats, and to investigate whether its effect on relieving inflammatory pain is associated with regulation of P2X3 receptor. Materials and methodsThe analgesic effects of SGE on CFA-induced inflammatory pain rats were evaluated by measuring mechanical pain threshold, thermal pain threshold, and motor coordination ability. The mechanisms of SGE in relieving inflammatory pain were explored by detecting inflammatory factors levels, NF-κB, COX-2 and P2X3 expression, and were further verified by addition of P2X3 receptor agonist (α, β me-ATP). ResultsOur results revealed that SGE can notably increase the mechanical pain threshold and thermal pain threshold of CFA-induced inflammatory pain rats, and markedly alleviate the pathological damage in DRG. SGE could suppress the release of inflammatory factors including IL-1β, IL-6, TNF-α and restrain the expression of NF-κB, COX-2 and P2X3. Moreover, α, β me-ATP further exacerbated the inflammatory pain of CFA-induced rats, while SGE could markedly raise the pain thresholds and relieve inflammatory pain. SGE could attenuate the pathological damage, inhibit P2X3 expression, inhibit the elevation of inflammatory factors caused by α, β me-ATP. SGE can also inhibit NF-κB and ERK1/2 activation caused by α, β me-ATP, and inhibit the mRNA expression of P2X3, COX-2, NF-κB, IL-1β, IL-6 and TNF-α in DRG of rats induced by CFA coupled with α, β me-ATP. ConclusionsIn summary, our research indicated that SGE could alleviate CFA-induced inflammatory pain by suppression of P2X3 receptor.

Effects of RNA M6A demethylase ALKBH5 gene deficiency on morphology and function of cerebellum in aged mice

Objective: To investigate the effects of RNA m6A demethylase ALKBH5 gene deficiency on cerebellar morphology and function in the aged mice, and to explore the role of ALKBH5 in cerebellar degeneration. Methods: Western blot was performed to detect the protein level of ALKBH5 in the cerebellum of wild-type mice of various ages. The expression of NeuN, Calbindin-D28K, MAP2, GFAP and other proteins in the cerebella of middle-aged (12-month-old) and aged (18-month-old) wild-type mice and ALKBH5-/- mice was examined using immunohistochemistry. The balance beam test and gait analysis were performed to test the balance ability and motor coordination of the mice. Results: With aging of the mice, the expression of ALKBH5 in the cerebellum increased gradually in an age-dependent manner. In the aged mice, but not middle-aged mice, the body weight, whole brain weight and cerebellum weight of ALKBH5-/- mice decreased by 15%, 10% and 21%, respectively (P<0.05). The expression of ALKBH5 in the Purkinje cells was much higher than that in other types of neural cells. Correspondingly, ALKBH5-deficiency caused 40% reduction in the number of Purkinje cells, as well as the length and density of neuronal dendrites in the aged mice (P<0.01). In addition, the time for the aged ALKBH5-/- mice to pass the balance beam was 70% longer than that of the wild type mice of the same age, with unstable gaits (P<0.01). Conclusions: Gene deficiency of RNA m6A demethylase ALKBH5 causes cerebellar atrophy, Purkinje neuron loss and damage in the aged mice. These changes eventually affect mice's motor coordination and balance ability. These results suggest that imbalanced RNA m6A methylation may lead to neurodegenerative lesions in the cerebellum of mice.

Excitatory and Inhibitory Descending Commissural Interneurons Differentially Integrate Supraspinal and Segmental Sensory Signals.

The limited information about how descending inputs from the brain and sensory inputs from the periphery use spinal cord interneurons (INs) is a major barrier to understanding how these inputs may contribute to motor functions under normal and pathologic conditions. Commissural interneurons (CINs) are a heterogeneous population of spinal INs that has been implicated in crossed motor responses and bilateral motor coordination (ability to use the right and left side of the body in a coordinated manner) and, therefore, are likely involved in many types of movement (e.g., dynamic posture stabilization, jumping, kicking, walking). In this study, we incorporate mouse genetics, anatomy, electrophysiology, and single-cell calcium imaging to investigate how a subset of CINs, those with descending axons called dCINs, are recruited by descending reticulospinal and segmental sensory signals independently and in combination. We focus on two groups of dCINs set apart by their principal neurotransmitter (glutamate and GABA) and identified as VGluT2+ dCINs and GAD2+ dCINs. We show that VGluT2+ and GAD2+ dCINs are both extensively recruited by reticulospinal and sensory input alone but that VGluT2+ and GAD2+ dCINs integrate these inputs differently. Critically, we find that when recruitment depends on the combined action of reticulospinal and sensory inputs (subthreshold inputs), VGluT2+ dCINs, but not GAD2+ dCINs, are recruited. This difference in the integrative capacity of VGluT2+ and GAD2+ dCINs represents a circuit mechanism that the reticulospinal and segmental sensory systems may avail themselves of to regulate motor behaviors both normally and after injury.SIGNIFICANCE STATEMENT The way supraspinal and peripheral sensory inputs use spinal cord interneurons is fundamental to defining how motor functions are supported both in health and disease. This study, which focuses on dCINs, a heterogeneous population of spinal interneurons critical for crossed motor responses and bilateral motor coordination, shows that both glutamatergic (excitatory) and GABAergic (inhibitory) dCINs can be recruited by supraspinal (reticulospinal) or peripheral sensory inputs. Additionally, the study demonstrates that in conditions where the recruitment of dCINs depends on the combined action of reticulospinal and sensory inputs, only excitatory dCINs are recruited. The study uncovers a circuit mechanism that the reticulospinal and segmental sensory systems may avail themselves of to regulate motor behaviors both normally and after injury.

Effect of 40 Hz light flicker on behaviors of adult C57BL/6J mice

40 Hz light flicker can activate multiple brain regions of wild-type mice. However, there are no systematic studies on the behavioral effects of 40 Hz light flicker on wild-type mice. Adult wild-type C57BL/6J mice were treated with 40 Hz light flicker (200 lx, 40 Hz, 1 h/day for 3 weeks) to evaluate its effects on several behaviors, including mood, locomotor activity, memory, social interaction, mechanical pain, and sense of smell. In the open field test, the elevated zero-maze test, forced swimming test, and tail suspension test, 40 Hz mice showed no anxiety and depression-like behaviors. In the rotarod test, no differences were found between the anti-fatigue ability and motor coordination ability. In memory-related tests, 40 Hz mice showed the short-term cognitive enhancement in the novel object recognition test. Interestingly, 40 Hz mice showed no enhanced the long-term memory performance in the contextual fear conditioning test, and tone-cued fear conditioning test. Besides, 40 Hz mice increased their exploration of social cues that were unfamiliar to them and differed significantly from their own experiences. In terms of sensory abilities, 40 Hz mice had unchanged pain sensitivity in the von Frey fiber test and significant enhancement in the olfactory ability in the food-seeking test. In conclusion, this 40 Hz light stimulation paradigm has high safety and can improve the specific behavioral ability, which provides a theoretical basis for the future use of 40 Hz light flicker as a disease prevention or treatment method.

Ogt Deficiency Induces Abnormal Cerebellar Function and Behavioral Deficits of Adult Mice through Modulating RhoA/ROCK Signaling.

Previous studies have shown the essential roles of O-GlcNAc transferase (Ogt) and O-GlcNAcylation in neuronal development, function and neurologic diseases. However, the function of Ogt and O-GlcNAcylation in the adult cerebellum has not been well elucidated. Here, we have found that cerebellum has the highest level of O-GlcNAcylation relative to cortex and hippocampus of adult male mice. Specific deletion of Ogt in granule neuron precursors (GNPs) induces abnormal morphology and decreased size of the cerebellum in adult male Ogt deficient [conditional knock-out (cKO)] mice. Adult male cKO mice show the reduced density and aberrant distribution of cerebellar granule cells (CGCs), the disrupted arrangement of Bergman glia (BG) and Purkinje cells. In addition, adult male cKO mice exhibit aberrant synaptic connection, impaired motor coordination, and learning and memory abilities. Mechanistically, we have identified G-protein subunit α12 (Gα12) is modified by Ogt-mediated O-GlcNAcylation. O-GlcNAcylation of Gα12 facilitates its binding to Rho guanine nucleotide exchange factor 12 (Arhgef12) and consequently activates RhoA/ROCK signaling. RhoA/ROCK pathway activator LPA can rescue the developmental deficits of Ogt deficient CGCs. Therefore, our study has revealed the critical function and related mechanisms of Ogt and O-GlcNAcylation in the cerebellum of adult male mice.SIGNIFICANCE STATEMENT Cerebellar function are regulated by diverse mechanisms. To unveil novel mechanisms is critical for understanding the cerebellar function and the clinical therapy of cerebellum-related diseases. In the present study, we have shown that O-GlcNAc transferase gene (Ogt) deletion induces abnormal cerebellar morphology, synaptic connection, and behavioral deficits of adult male mice. Mechanistically, Ogt catalyzes O-GlcNAcylation of Gα12, which promotes the binding to Arhgef12, and regulates RhoA/ROCK signaling pathway. Our study has uncovered the important roles of Ogt and O-GlcNAcylation in regulating cerebellar function and cerebellum-related behavior. Our results suggest that Ogt and O-GlcNAcylation could be potential targets for some cerebellum-related diseases.

Optimizing Fine Motor Coordination, Selective Attention and Reaction Time in Children: Effect of Combined Accuracy Exercises and Visual Art Activities.

Optimizing fine motor coordination and cognitive abilities in middle childhood through new intervention programs remains one of the most essential orientations in motor development and skills proficiency. The present study aims to identify the effect of a structure-based program intervention, combining motor accuracy exercises with visual art activities (ceramics, pottery, and creations using raw materials), on fine motor coordination, selective attention, and reaction time in middle childhood. Sixty, right-handed typically developed male schoolchildren (age = 8.29 ± 0.74 years; body height = 130.25 ± 0.05 cm and body mass = 29.83 ± 4.68 kg; mean ± SD) volunteered to participate in the study. They were randomly assigned to a combined group intervention (MG) receiving a mixed program integrating visual art activities and motor accuracy exercises; an accuracy group intervention (AG) receiving oriented motor accuracy exercises; and a control group (CG). Selective attention, reaction time, and fine motor coordination skills (accuracy: darts throw; manual dexterity: grooved pegboard test) were measured. Repeated measure ANOVA, one-way ANOVA, and Kruskal-Wallis ANOVA were performed for analysis. The results showed higher changes in MG compared to AG in manual dexterity (p < 0.001), in motor accuracy (p < 0.001), and in reaction time (p < 0.01), but not in selective attention (p = 0.379). In addition, higher changes were recorded in AG compared to CG in motor accuracy (p < 0.05), reaction time (p < 0.05), and in selective attention (p < 0.01), but not in manual dexterity (p = 0.082). The structured combined program best assists accuracy, manual dexterity, reaction time, and selective attention. Therefore, instructors in sports academies and teachers at schools are advised to use the combined program in the training sessions and in the non-curricular activities, respectively, to improve fine motor coordination, selective attention, and reaction time in middle childhood.

Effective motor learning and coordination abilities of girls and boys aged 9-10

Introduction&#x0D; Learning is a complex and multi-dimensional process that depends on cognitive and emotional processes and the development of functional systems of the human body. One type of learning is motor learning. Its course and effects depend on many factors. One of the factors may be coordination abilities.&#x0D; Purpose&#x0D; The cognitive aim of the study is to assessment of the effectiveness (speed, efficiency) of learning complex motor activity in relation to the selected aspect of physical development (coordination abilities) of a selected group of girls and boys.&#x0D; Materials and methods&#x0D; The study was conducted among 73 children (44 boys and 29 girls) aged 9 - 10 years. Authors used the pedagogical experiment method and the research tool was the program for learning how to juggle three tennis balls. To assess the selected aspects of physical development we chose the direct categorized observation method. As research tools Authors used the Vienna Test System (VTS) and 2hand test.&#x0D; Results&#x0D; Boys achieved a higher average level of eye-hand coordination (2-hand test), but the differences between them and girls were not statistically significant. Girls achieved, on average, higher results than boys in terms of precise hand movements (MLS test). Statistically significant differences in their favor occurred in most of the tests performed.&#x0D; Conclusion&#x0D; There are functions between the speed and efficiency of learning a complex motor activity and coordination abilities. They are more pronounced than boys. It is important to develop coordination abilities in the didactic process to support motor learning.&#x0D; &#x0D;