Introduction. The era of the industrial revolution 4.0 is a challenge for administrators and lecturers in higher education. Changes and developments in technology and types of work occur quickly, forcing educational institutions to change according to these changes. Vocational higher education managed by the government has specificities in organising and managing their education. Aim. This research aims to determine the relationship and effect between organisational culture on leadership, between leadership on lecturer motivation, and between organisational culture on lecturer motivation in the institutions of Government Vocational Higher Education (GVHE). Methodology and research methods. This is explanatory research, with samples taken from lecturers of GVHEs and the information collected through the questionnaires. The partial least squares (PLS) method was used to measure relationships and effects between the variables, such as organisational culture on leadership, leadership on lecturer motivation, and organisational culture on lecturer motivation. Results and scientific novelty. The results showed that organisational culture and leadership had a significant relationship, and organisational culture had a moderate effect on leadership. Leadership has a significant relationship to lecturer motivation. Organisational culture has an insignificant relationship with lecturer motivation. However, organisational culture and leadership have a considerable influence on lecturer motivation. Practical significance. This research gives recommendations to leaders of GVHEs to adapt to the current challenge, be more flexible in their organisational culture, and have good leadership to improve the lecturer motivation, which impacts their performance.