The mechanical reinforcement of rubber by carbon black (CB) depends strongly on its the size and topography of CB clusters. However, the underlying mechanisms remain largely unexplored. This study uses atomic force microscopy (AFM) to probe interfacial properties at the nanoscale to elucidate the influence of the CB topological structure on macroscopic mechanical properties. A substantial amount of high-modulus bound rubber is found inside the CB aggregates, particularly in highly branched ones. This phenomenon plays a critical role in reinforcement, as corroborated by quantitative AFM nanomechanics, chain segment motion results and theoretical calculations. A quantitative analysis of the filler network reveals that the branched chain structure effectively reduces the packing spacing and improves the stress transfer efficiency.