The electric field and space charge dynamics characteristics in oil under impulse voltage are key factors to the transformer insulation design, which are mainly obtained through simulation, falling short of experimental verifications. Applying the Kerr electro-optic method, the present paper carried out an actual measurement for the electric field characteristics in transformer oil under impulse voltage and managed to obtain their correlations with an electrode material, voltage amplitude, and wave-front time, respectively. First, it was found that, under impulse voltage and in pure transformer oil, the aluminum electrode had the largest amount of charge injection, followed by stainless steel and then copper, which was attributed to the different work functions of metal elements in electrodes. Second, with the increase of voltage amplitude, the effect of the space charge on the intermediate electric field of the oil gap was first enhanced, then weakened, and eventually enhanced. Third, as the wave-front time was prolonged, the peak of electric field in transformer oil showed a downward trend. For instance, the peak value of the electric field was reduced by 17.6 % when the wave-front time was increased from 0.5 μs to 40.0 μs. The reason is that when the wave-front time is longer, the amount of injected charge grows larger and the weakening effect becomes stronger. Fourth, a charge dynamic motion model in transformer oil under impulse voltage was established to demonstrate its spatial-temporal effects on the electric field. Besides, this paper has clarified the source of the negative and positive charges in the transformer oil under impulse voltage by using the theories of field emission and field ionization. Moreover, the variation of electron mobility over-voltage amplitude, wave-front time, and spatial distribution was worked out to present the quantitative results of its motion process.