Arrangement Of Motifs Research Articles

Evolutionary Analysis and Catalytic Function of LOG Proteins in Plants

Background: The plant hormone cytokinin is a conserved regulator of plant development. LONELY GUY (LOG) proteins are pivotal in cytokinin biosynthesis. However, their origin, evolutionary history, and enzymatic characteristics remain largely uncharacterized. Methods: To elucidate LOG family evolution history and protein motif composition, we conducted phylogenetic and motif analyses encompassing representative species across the whole green plant lineage. Catalytic activity and structure analysis were conducted to thoroughly characterize the LOG proteins. Results: Our phylogeny showed that LOG proteins could be divided into five groups and revealed three major duplication events giving rise to four distinct groups of vascular LOG proteins. LOG proteins share a conserved structure characterized by a canonical motif arrangement comprising motifs 1, 2, 3, 4, 5, 6, and 7. Two significant changes in LOG motif composition occurred during the transition to land plants: the emergence of motif 3 in charophyte LOG sequences and the subsequent acquisition of motif 8 at the C-terminus of LOG proteins. Enzymatic assays demonstrated that LOG proteins can be classified into two groups based on their enzyme activity. One group act as cytokinin riboside 5′-monophosphate phosphoribohydrolase and primarily convert iPRMP to iP, while the other group act as 5′-ribonucleotide phosphohydrolase, and preferentially produce iPR from the same substrates. TaLOG5-4A1, TaLOG5-4A2, TaLOG5-5B2, and TaLOG5-D1 shared conserved residues in the critical motif and were predicted to have similar protein structures, but displayed distinct enzymatic activities. Conclusions: Our findings provide a comprehensive overview of LOG protein phylogeny and lay a foundation for further investigations into their functional diversification.

Genome-Wide Identification and Expression Analysis of the REF Genes in 17 Species

Natural rubber production currently relies heavily on a single species, Hevea brasiliensis, underscoring the urgent need to identify alternative sources to alleviate the strain on natural rubber production. The rubber elongation factor (REF) and small rubber particle protein (SRPP), both members of the REF/SRPP gene family, are crucial for natural rubber biosynthesis. However, research on the REF gene has predominantly focused on H. brasiliensis and Taraxacum kok-saghyz. We conducted a comprehensive genome-wide identification and characterization of the REF gene, identifying 87 REF protein sequences across 17 plants species. We observed a significant increase in the copy numbers and expression of REF genes in rubber-producing plants. Notably, in H. brasiliensis, T. kok-saghyz, Eucommia ulmoides, Lactuca sativa, and other rubber-yielding species, the number of REF genes has markedly increased. Furthermore, some REF genes in H. brasiliensis form a distinct clade in phylogenetic analyses and exhibit differences in conserved motif arrangements and tertiary protein structures compared to other REF genes. These findings suggest that REF genes in rubber-producing plants may have undergone independent evolution, leading to changes in copy number and structure. These alterations could contribute to the production of natural rubber in these species. The results of this study provide a scientific basis for further research into the mechanisms of rubber production in plants and for identifying potential rubber-producing species.

Curved Nanointerface Controls the Chiral Effect on Peptide Fibrillation.

Nanostructures with varying functionalities have been engineered to modulate the fibrillation of amyloid-β (Aβ) peptides. Nevertheless, the chirality effect at the curved nanointerfaces is seldom dissected. In this study, we systematically explored the curvature-modulated chiral effect on the regulation of Aβ1-42 fibrillation by using l/d-penicillamine-gold nanoparticles (l/d-PGNPs). According to the microscopic and spectroscopic analyses, Aβ1-42 fibrillation can be effectively suppressed by more curved (0.2 nm-1, 1/r) d-nanointerface (d-PGNPs5) with notable chiral selectivity, even at a low inhibitor/peptide (I/P) molar ratio (1:100). A greatly alleviated cytotoxic effect of Aβ1-42 peptides after the inhibition process is also revealed. The highly curved nanointerface drives the formation of multiple hydrogen bonds and promotes electrostatic interactions with Aβ1-42. Importantly, the curved d-nanointerface controls well the spatial arrangement of Pen motifs, making it incompatible with the fibrillation direction of Aβ1-42 and thus gaining enhanced efficiency on amyloid fibrillar modulation. This study provides valuable insights into the interactions between chirality and peptide-nanointerface effects, which are crucial for the development of inhibitors in anti-β-amyloidosis.

Identification and Functional Characterization of Abiotic Stress Tolerance-Related PLATZ Transcription Factor Family in Barley (Hordeum vulgare L.).

Plant AT-rich sequence and zinc-binding proteins (PLATZs) are a novel category of plant-specific transcription factors involved in growth, development, and abiotic stress responses. However, the PLATZ gene family has not been identified in barley. In this study, a total of 11 HvPLATZs were identified in barley, and they were unevenly distributed on five of the seven chromosomes. The phylogenetic tree, incorporating PLATZs from Arabidopsis, rice, maize, wheat, and barley, could be classified into six clusters, in which HvPLATZs are absent in Cluster VI. HvPLATZs exhibited conserved motif arrangements with a characteristic PLATZ domain. Two segmental duplication events were observed among HvPLATZs. All HvPLATZs were core genes present in 20 genotypes of the barley pan-genome. The HvPLATZ5 coding sequences were conserved among 20 barley genotypes, whereas HvPLATZ4/9/10 exhibited synonymous single nucleotide polymorphisms (SNPs); the remaining ones showed nonsynonymous variations. The expression of HvPLATZ2/3/8 was ubiquitous in various tissues, whereas HvPLATZ7 appeared transcriptionally silent; the remaining genes displayed tissue-specific expression. The expression of HvPLATZs was modulated by salt stress, potassium deficiency, and osmotic stress, with response patterns being time-, tissue-, and stress type-dependent. The heterologous expression of HvPLATZ3/5/6/8/9/10/11 in yeast enhanced tolerance to salt and osmotic stress, whereas the expression of HvPLATZ2 compromised tolerance. These results advance our comprehension and facilitate further functional characterization of HvPLATZs.

Cell-type-directed design of synthetic enhancers.

Transcriptional enhancers act as docking stations for combinations of transcription factors and thereby regulate spatiotemporal activation of their target genes1. It has been a long-standing goal in the field to decode the regulatory logic of an enhancer and to understand the details of how spatiotemporal gene expression is encoded in an enhancer sequence. Here we show that deep learning models2-6, can be used to efficiently design synthetic, cell-type-specific enhancers, starting from random sequences, and that this optimization process allows detailed tracing of enhancer features at single-nucleotide resolution. We evaluate the function of fully synthetic enhancers to specifically target Kenyon cells or glial cells in the fruit fly brain using transgenic animals. We further exploit enhancer design to create 'dual-code' enhancers that target two cell types and minimal enhancers smaller than 50 base pairs that are fully functional. By examining the state space searches towards local optima, we characterize enhancer codes through the strength, combination and arrangement of transcription factor activator and transcription factor repressor motifs. Finally, we apply the same strategies to successfully design human enhancers, which adhere to enhancer rules similar to those of Drosophila enhancers. Enhancer design guided by deep learning leads to better understanding of how enhancers work and shows that their code can be exploited to manipulate cell states.

Media Development for Karawo Motif Design Using Genetic Algorithm Based on Gorontalo Regional Culture

The lack of karawo motif designs with a Gorontalo regional cultural philosophy causes karawo artisans to lack ideas to design karawo motifs in various design variations. One solution to overcome this is to create a media that can make several variations of the karawo motif design. The method used is an experimental method that consists of (1) preparing image data, (2) making geometric transformations, (3) developing a system using genetic algorithms, (4) implementation, (5) testing system, and Analyze media effectiveness. The drafting system is carried out using one image data, two image data, and three karawo motif image data. These data are then subjected to geometric transformations such as 450 rotation, horizontal flip, and vertical flip. Furthermore, the selection of transformation treatments is carried out by optimizing the fitness function in the genetic algorithm. Testing of the karawo motif arrangement system was carried out on one image data, two image data, and three image data. The N-Gain statistical analysis results from the initial trials and extended trials revealed percentages of 66.9% and 73%, respectively. These figures indicate the effectiveness of the developed media in enhancing the skills and knowledge of artisans in creating karawo motif designs.

Genome-wide identification of CaARR-Bs transcription factor gene family in pepper and their expression patterns under salinity stress

In plants, ARRs-B transcription factors play a crucial role in regulating cytokinin signal transduction, abiotic stress resistance, and plant development. A number of adverse environmental conditions have caused severe losses for the pepper (Capsicum annuum L.)—a significant and economically important vegetable. Among the transcription factors of the type B-ARRs family, multiple members have different functions. In pepper, only a few members of the ARRs-B family have been reported and characterized. The current study aimed to characterize ARRs-B transcription factors in C. annuum, including phylogenetic relationships, gene structures, protein motif arrangement, and RT-qPCR expression analyses and their role in salinity stress. In total, ten genes encode CaARRs-B transcription factors (CaARR1 to CaARR10) from the largest subfamily of type-B ARRs were identified in C. annum. The genome-wide analyses of the CaARRs-B family in C. annuum were performed based on the reported ARRs-B genes in Arabidopsis. An analysis of homologous alignments of candidate genes, including their phylogenetic relationships, gene structures, conserved domains, and qPCR expression profiles, was conducted. In comparison with other plant ARRs-B proteins, CaARRs-B proteins showed gene conservation and potentially specialized functions. In addition, tissue-specific expression profiles showed that CaARRs-B genes were differentially expressed, suggesting functionally divergent. CaARRs-B proteins had a typical conserved domain, including AAR-like (pfam: PF00072) and Myb DNA binding (pfam: PF00249) domains. Ten of the CaARRs-B genes were asymmetrically mapped on seven chromosomes in Pepper. Additionally, the phylogenetic tree of CaARRs-B genes from C. annuum and other plant species revealed that CaARRs-B genes were classified into four clusters, which may have evolved conservatively. Further, using quantitative real-time qRT-PCR, the study assessed the expression patterns of CaARRs-B genes in Capsicum annuum seedlings subjected to salt stress. The study used quantitative real-time qRT-PCR to examine CaARRs-B gene expression in Capsicum annuum seedlings under salt stress. Roots exhibited elevated expression of CaARR2 and CaARR9, while leaves showed decreased expression for CaARR3, CaARR4, CaARR7, and CaARR8. Notably, no amplification was observed for CaARR10. This research sheds light on the roles of CaARRs-B genes in pepper’s response to salinity stress. These findings enrich our comprehension of the functional implications of CaARRs-B genes in pepper, especially in responding to salinity stress, laying a solid groundwork for subsequent in-depth studies and applications in the growth and development of Capsicum annuum.

Discussion: “An Upper Palaeolithic Proto-writing System and Phenological Calendar” by Bennett Bacon et al. (2023)

A recent work by Bacon et al. (2023) proposes to interpret a large part of Palaeolithic art as an ethological calendar. They argue that by studying the association of certain signs (dots, lines, and Y-shapes) with an animal, it is possible to infer vital episodes such as reproduction, birth, and migration of the represented species. However, in the present article, we discuss some methodological errors made by the authors. For instance, they use a tracing to demonstrate the association between a mammoth and a series of lines at El Pindal, although this tracing is not faithful to the actual arrangement of the pictorial motifs in the cave. In Pair-non-Pair, Sotarriza, and Atxurra caves, the signs considered do not really exist. And in other cases, such as Altxerri, Covaciella, or Tito Bustillo, the signs have been misinterpreted. Important problems such as the lack of definition of “association” and various apriorisms and presentisms adopted by the authors are also exposed and discussed. In conclusion, this proposal lacks methodological support and it is not possible to conclude that an ethological calendar was present in Palaeolithic art.

Deciphering the Roles of Peanut (Arachis hypogaea L.) Type-One Protein Phosphatase (TOPP) Family in Abiotic Stress Tolerance

Dephosphorylation is one of the important mechanisms regulating signal transduction in plant growth and development and in response to abiotic stresses. Type-one protein phosphatases (TOPPs) catalyze a significant number of important dephosphorylation events in cells, and play essential roles in plant developmental regulations and multiple stress responses. Nevertheless, the knowledge regarding the peanut’s TOPP gene family remains extremely restricted. Thirteen TOPP genes (AhTOPP1-13) were discovered in the peanut genome database through the utilization of HMMER and BLASTP methods in this research. The thirteen AhTOPP genes were classed into three clades together with their Arabidopsis homologs based on phylogenetic tree, and mapped on nine of twenty chromosomes. The examination of gene compositions and protein patterns indicated resemblance in the structure of exons and introns, as well as the arrangement of motifs within the identical clade, which further reinforces the findings of phylogenetic analysis. All AhTOPP proteins possessed STPPase_N, Metallophos domains, and the core catalytic sites. Promoter analysis showed that the AhTOPP genes may be widely involved in peanut development, hormones, and stress response. The RNA-seq data revealed the presence of AhTOPP genes in twenty-two tissues, suggesting potential variations in the functionality of AhTOPP genes. Furthermore, drought and salt stresses induced the expression of multiple AhTOPP genes, including AhTOPP1, AhTOPP4, AhTOPP7, AhTOPP9, and AhTOPP13. It is worth mentioning that the AhTOPP genes’ expression could potentially be controlled by various transcription factors with different functions, including ERF, WRKY, MYB, and Dof. We will conduct specific functional studies on the peanut TOPP genes through transgenics in future research.

Structural similarity of human papillomavirus E4 and polyomaviral VP4 exhibited by genomic analysis of the common kestrel (Falco tinnunculus) polyomavirus

Polyomaviruses are widely distributed viruses of birds that may induce developmental deformities and internal organ disorders primarily in nestlings. In this study, polyomavirus sequence was detected in kidney and liver samples of a common kestrel (Falco tinnunculus) that succumbed at a rescue station in Hungary. The amplified 5025 nucleotide (nt) long genome contained the early (large and small T antigen, LTA and STA) and late (viral proteins, VP1, VP2, VP3) open reading frames (ORFs) typical for polyomaviruses. One of the additional putative ORFs (named VP4) showed identical localization with the VP4 and ORF-X of gammapolyomaviruses, but putative splicing sites could not be found in its sequence. Interestingly, the predicted 123 amino acid (aa) long protein sequence showed the highest similarity with human papillomavirus E4 early proteins in respect of the aa distribution and motif arrangement implying similar functions. The LTA of the kestrel polyomavirus shared <59.2% nt and aa pairwise identity with the LTA sequence of other polyomaviruses and formed a separated branch in the phylogenetic tree among gammapolyomaviruses. Accordingly, the kestrel polyomavirus may be the first member of a novel species within the Gammapolyomavirus genus, tentatively named Gammapolyomavirus faltin.

A STUDY ON TRADITIONAL MOTIFS AND TECHNIQUES OF DHABHLA AND ITS CHANGES OF KUTCH REGION

The primary purpose of the study is to evaluate the traditional motifs and techniques of Dhabhla and its change in the Kutch region. Kutch district of Gujarat is a religious centre for pilgrimage and is interesting for historical lovers. The weaving craft of Kutch has its origins in the barter system in the past time. Weavers were very dependent upon other communities to get work. Traditional weaving has been accepted from generation to generation; the passage of time has created newness in design to retain its interest. The Dhabhla was available in various traditional motifs based on the daily life of the weavers - mostly geometrical motifs. The weaving of Kutch is famous for its traditional intricate designs, arrangement of motifs, and rural-based handicrafts. Usually, the effect of delicacy, rich-vivid, and contrast color combinations are imparted in patterning. The Centre of weaving is Kutch and Sunder Nagar.

Alternative Splicing Outcomes Across an RNA-Binding Protein Concentration Gradient

Alternative splicing (AS) is a dynamic RNA processing step that produces multiple RNA isoforms from a single pre-mRNA transcript and contributes to the complexity of the cellular transcriptome and proteome. This process is regulated through a network of cis-regulatory sequence elements and trans-acting factors, most-notably RNA binding proteins (RBPs). The muscleblind-like (MBNL) and RNA binding fox-1 homolog (RBFOX) are two well characterized families of RBPs that regulate fetal to adult AS transitions critical for proper muscle, heart, and central nervous system development. To better understand how the concentration of these RBPs influences AS transcriptome wide, we engineered a MBNL1 and RBFOX1 inducible HEK-293 cell line. Modest induction of exogenous RBFOX1 in this cell line modulated MBNL1-dependent AS outcomes in 3 skipped exon events, despite significant levels of endogenous RBFOX1 and RBFOX2. Due to background RBFOX levels, we conducted a focused analysis of dose-dependent MBNL1 skipped exon AS outcomes and generated transcriptome wide dose–response curves. Analysis of this data demonstrates that MBNL1-regulated exclusion events may require higher concentrations of MBNL1 protein to properly regulate AS outcomes compared to inclusion events and that multiple arrangements of YGCY motifs can produce similar splicing outcomes. These results suggest that rather than a simple relationship between the organization of RBP binding sites and a specific splicing outcome, that complex interaction networks govern both AS inclusion and exclusion events across a RBP gradient.

Identification and characterization of Dof genes in Cerasus humilis.

Dof genes encode plant-specific transcription factors, which regulate various biological processes such as growth, development, and secondary metabolite accumulation. We conducted whole-genome analysis of Chinese dwarf cherry (Cerasus humilis) to identify ChDof genes and characterize the structure, motif composition, cis-acting elements, chromosomal distribution, and collinearity of these genes as well as the physical and chemical properties, amino acid sequences, and phylogenetic evolution of the encoded proteins. The results revealed the presence of 25 ChDof genes in C. humilis genome. All 25 ChDof genes could be divided into eight groups, and the members of the same group had similar motif arrangement and intron-exon structure. Promoter analysis showed that cis-acting elements responsive to abscisic acid, low temperature stress, and light were dominant. Transcriptome data revealed that most ChDof genes exhibited tissue-specific expression. Then, we performed by qRT-PCR to analyze the expression patterns of all 25 ChDof genes in fruit during storage. The results indicated that these genes exhibited different expression patterns, suggesting that they played an important role in fruit storage. The results of this study provide a basis for further investigation of the biological function of Dof genes in C. humilis fruit.

Characterization of the B-BOX gene family in pepper and the role of CaBBX14 in defense response against Phytophthora capsici infection

The B-box (BBX) transcription factors are widely implicated in plant growth, development, and response to various biotic and abiotic stresses. However, their roles in the response of pepper to Phytophthora capsici infection (PCI) remain largely unexplored. Here, we report a total of 25 CaBBX genes with an uneven distribution were identified in pepper genome, and their characteristics, phylogenetic relationships, gene structures, conserved domains, and expression profiles were validated. CaBBXs were classified into five major clades (I to V) based on their phylogenetic relationships and conserved domains (presence of one or two B­box domains and a CCT domain). Gene duplication analysis demonstrated that there are two segmental duplication events but no tandem duplication event within pepper genome. Conserved motif and gene structure analysis revealed that the CaBBXs in the same clade have relatively similar motif arrangements and exon-intron patterns. Expression analysis revealed that the CaBBX genes have different expression levels in various tissues, and some of which were significantly induced during PCI and exogenous salicylic acid (SA) treatment. Among them, CaBBX14 displayed remarkable changed expression during PCI and SA treatment. The silencing of CaBBX14 increases pepper susceptibility to PCI, and also decreases in SA content and expression of pathogenesis-related (PR) and SA-related genes compared with control plants. Together, these findings advance our knowledge base on biological functions of CaBBXs in pepper during PCI through the SA signaling pathway, and we provide an example demonstrating that the potential of CaBBX14 to improve pepper resistance to PCI.

From circle packings to constellation patterns

We describe a process to convert a circle packing into a dense arrangement of interconnected star motifs. The conversion process is a simple and robust point-joining algorithm, and the resulting family of geometric patterns shares properties with some Islamic star patterns. We demonstrate how some traditional patterns correspond to packings generated from equilateral tilings. We also apply the process to compact packings, which creates balanced versions of some traditional patterns, some new patterns, and visual effects not found in traditional Islamic patterns.

Deciphering the influence of genome architecture in minimized bacteria

Stochastic gene expression begins with the repeated transcription of DNA by RNA polymerase (RNAP) to create the various functional forms (rRNA, tRNA, mRNA, and sRNA) of the bacterial transcriptome. The initiation and termination of transcriptional events are associated with the genome architecture, the local arrangement of genetic features along the circular bacterial genome identified via sequence motifs (promoters, Shine-Dalgarno, gene open-reading frames, and transcription termination sites, etc.). The local arrangement of these sequence motifs form operon-like regions, known as transcriptional units, directly controlling the expression of encoded RNA species. The stochastic activity between RNAP and the genetic features of a transcription unit results in differential transcription forming RNA isoforms, various RNA transcripts from a similar region of different lengths. Locations of the genetic features that define the transcription unit boundaries were predicted using structural and bioinformatic analysis, and then integrated to predict transcriptional units within two minimized bacteria, JCVI-syn1.0 and JCVI-syn3A. The theoretical predictions agree well with observations of expression obtained using Oxford Nanopore Technologies Direct RNA sequencing for Syn1.0 the precursor of Syn3A. Comparisons of the computational and experimental results help to provide new insight into transcriptional activity within the organisms, resolve the distribution of RNA isoform complexity evolved from key genomic regions such as those involved in membrane transport and cell division, motivate the investigation into the post-transcriptional activity of RNA, and explain the impact on gene expression from the genome reduction of Syn1.0 to Syn3A.

De novo protein fold design through sequence-independent fragment assembly simulations

De novo protein design generally consists of two steps, including structure and sequence design. Many protein design studies have focused on sequence design with scaffolds adapted from native structures in the PDB, which renders novel areas of protein structure and function space unexplored. We developed FoldDesign to create novel protein folds from specific secondary structure (SS) assignments through sequence-independent replica-exchange Monte Carlo (REMC) simulations. The method was tested on 354 non-redundant topologies, where FoldDesign consistently created stable structural folds, while recapitulating on average 87.7% of the SS elements. Meanwhile, the FoldDesign scaffolds had well-formed structures with buried residues and solvent-exposed areas closely matching their native counterparts. Despite the high fidelity to the input SS restraints and local structural characteristics of native proteins, a large portion of the designed scaffolds possessed global folds completely different from natural proteins in the PDB, highlighting the ability of FoldDesign to explore novel areas of protein fold space. Detailed data analyses revealed that the major contributions to the successful structure design lay in the optimal energy force field, which contains a balanced set of SS packing terms, and REMC simulations, which were coupled with multiple auxiliary movements to efficiently search the conformational space. Additionally, the ability to recognize and assemble uncommon super-SS geometries, rather than the unique arrangement of common SS motifs, was the key to generating novel folds. These results demonstrate a strong potential to explore both structural and functional spaces through computational design simulations that natural proteins have not reached through evolution.

Genome-wide characterization and expression profiling of GASA gene family in Triticum turgidum ssp. durum (desf.) husn. (Durum wheat) unveils its involvement in environmental stress responses

Family members within the plant-specific gibberellic acid-stimulated Arabidopsis (GASA) gene serve a crucial role in plant growth and development, particularly in flower induction and seed development. Through a genome-wide analysis of Triticum turgidum ssp. Durum (durum wheat), we identified 19 GASA genes, designated as TdGASA1‒19. Moreover, the chromosomal locations, exon–intron distribution and the physiochemical properties of these genes were determined and the subcellular localization of their encoded proteins was estimated. Analyses of their domain structure, motif arrangements, and phylogeny revealed four distinct groups that share a conserved GASA domain. Additionally, a real-time q-PCR analysis revealed differential expression patterns of TdGASA genes in various tissues (including leaves, roots, stems, and seeds) and in response to salinity, osmotic stress, and treatment with exogenous phytohormones (abscisic and gibberellic acid), implying that these genes may play a role in the growth, development, and stress responses of Triticum turgidum. Heterologous expression of TdGASA1, TdGASA4, TdGASA14, and TdGASA19 in Saccharomyces cerevisiae improved its tolerance to salt, osmotic, oxidative, and heat stresses, which suggests the involvement of these genes in abiotic stress tolerance mechanisms. The present study is the first to identify and analyze the expression profile of T. turgidum GASA genes, therefore offering novel insights for their further functional characterization, which may serve as a novel resource for molecular breeding of durum wheat.

Research Methods of Theatrical Costume: Ukrainian Experience

The purpose of the article includes the theoretical context of theatrical costume research. After all, correctly selected and systematically organised methods provide a scientific basis, and therefore success in discourse. Classical methods of art history that arose in the 19th century were chosen in order to realise the outlined goal. Those methods to nowadays have not lost their relevance, namely, formal-stylistic, iconographic, and iconological since we were interested in the possibility of their application for the study of stage costume. The methods used in the article were primarily aimed to identify a common algorithm in the study of a separate narrow subject of research. By applying the historical-comparative and typological approaches to the above-mentioned methods, their universal possibilities were revealed, and thus the undiscovered potential in the process of performing the analysis of theatrical costume. The novelty of the article is evidenced by the theoretical elaboration of the content of each of the three specified methods, supported by historical facts-examples from the history of the costumes of performances staged in different years in Ukraine. The conclusions of the study are based on the step-by-step application of the three specified methods of classical art history for the analysis of theatrical costumes and the performance. The formal analysis gives a comprehensive description of the costume in its components, colour, silhouette, and architecture. Iconography opens up opportunities for classification as an elementary arrangement of concepts, motifs, and themes. Iconology allows to extend the classification scale to typological and take the next step through synchronic or diachronic contextuality.&#x0D; Key words: theatrical costume, art history, formal-stylistic method, iconography, iconology.

Genomic analysis of sugar transporter genes in peanut (Arachis hypogaea): Characteristic, evolution and expression profiles during development and stress

Peanut (Arachis hypogaea L.) is an economically significant crop with aerial cleistogamous flowers and subterranean geocarpic fruit (pods). The formation of peanut pod requires movement of the embryo from air to ground and then development in the soil, which is a complex biological process involving transport and accumulation of sugars. Sugar transport proteins (STP) mediate the transport of monosaccharides in various physiological processes, including fertilization, ovary formation, and seed development. In this study, a total of 36 AhSTP genes (AhSTP1–36) containing the conserved sugar_tr motif were identified in the A. hypogaea genome. Phylogenetic analysis revealed that AhSTP genes were classified into four clades, and the arrangement of motifs in AhSTP proteins was similar within clades. Synteny analysis revealed that segmental duplication events have played an important role in the expansion of STP genes in peanut, and chromosome rearrangements might have facilitated the exchange of STP genes between the A and B sub-genomes. Transcriptome analyses revealed that the expression patterns of AhSTP genes varied among tissues. Hormone and abiotic stress treatments could up-regulate or down-regulate the expression of AhSTP genes, and low temperature had a major effect on the expression of most AhSTP genes. Four AhSTP genes (AhSTP3, AhSTP9, AhSTP19, and AhSTP28) were specifically expressed in the pod, indicating that these genes might be involved in pod formation and development in peanut. The unique expression of these four genes during pod construction and development was confirmed in two different type cultivars using quantitative real-time PCR analysis. Our findings provide new insights into the STP gene family in peanut and will aid future functional studies of AhSTP genes.