Aim: This study intends to focus on assessment of knowledge and attitude regarding vitamin A deficiency among mothers of under five children in selected Paediatric OPD, West Bengal. 100 participants were selected through purposive sampling technique. Materials and Methods: Data were selected through interviewing technique using structured interview schedule. Results: It revealed that most of the mothers (55%) had average knowledge, (28%) mothers had good knowledge, and (17%) mothers had poor knowledge. Knowledge score ranged from 1-12 with mean 7.1 and median 7.5 and calculated SD was 2.4. It also revealed that most of the mothers (60%) of under five children had good attitude, (34%) mothers had average attitude and (6%) mothers had poor attitude. Attitude score ranged from 1-10 with mean 7.6 and median 7.1 and calculated SD was 1.8. There was significant association with knowledge score and selected demographic variables, like- age of mothers, educational qualification, distance of health centre from home and any missed dose of baby. There was also significant association between attitude score with selected demographic variables like- educational qualification, habitant, distance of health centre and visited health centre along with children. The study findings have significant implications for nursing administration and research.