Moth Bean Research Articles

Summer pulses as sources of green manure and soil cover in the U.S. Southern Great Plains

Winter wheat (Triticum aestivum L.) – summer fallow rotations in the Southern Great Plains (SGP) have numerous sustainability issues, such as low precipitation use efficiencies and increased soil erosion. Replacing summer fallow with a legume grown as a green manure would help reduce soil erosion, improve precipitation use efficiency, and add nitrogen (N) credits for the subsequent wheat crop. A two-year field experiment evaluated the capabilities of three summer pulses: soybean [Glycine max (L.) Merr.], moth bean [Vigna aconitifolia (Jacq.) Marechal], and tepary bean [Phaseolus acutifolius (A.) Gray] to serve as green manures and soil cover. We examined their performance across two row spacings (38 and 76 ​cm) and two moisture regimes (rainfed and irrigated). Narrow row spacing (38 ​cm) provided greater canopy cover, aboveground biomass, and N accumulation than the broad row spacing (76 ​cm) during the early growing season. Among species, tepary bean demonstrated consistent and higher canopy cover early in the season. Soybean produced the highest aboveground biomass (5,327–8,855 ​kg ​ha−1) and N accumulation (115–269 ​kg ​ha−1) among the three pulses. Multilinear regression (MLR) models suggested that canopy height and canopy cover could estimate both aboveground biomass (R2 ​= ​0.62) and N accumulation (R2 ​= ​0.55) for tested pulses. Based on results, soybean was the most promising choice among tested pulses, considering its higher aboveground biomass and N accumulation. Modelling studies to simulate growth of these crops with long-term weather scenarios are encouraged to identify the most reliable cover crop for different areas of the SGP.

ELUCIDATING THE HOST PREFERENCE BY THE PULSE BEETLE <i>CALLOSOBRUCHUS CHINENSIS</i> (L)

Callosobruchus chinensis (Bruchidae: Coleoptera) known as pulse beetle is a serious pest of pulses causing economic losses. The present work reports the effect of pulses on life cycle parameters (oviposition, emergence, longevity, total development period) of Callosobruchus chinensis (L.), and also on the nutritional loss incurred by it. Grains of seven pulses viz. Vigna radiata (green gram), Vigna aconitifolia (moth bean), Cicer arietinum (desi chickpea), Vigna unguiculata (cowpea), Cajanus cajan (pigeon pea), Vigna mungo (black gram) and Pisum sativum (yellow pea) were used as host with three replications. The results exhibit alterations in the developmental period as it was recorded least in green gram (23± 0.58 days) and maximum in peas (42± 1 days). The number of eggs were maximum on pea (310± 2) and least in moth (180± 2). Maximum longevity was recorded on green grams (16 days) and least on pea (9± 1 days). A significant correlation between weight loss and adult emergence was observed. Loss of nutritional content like protein and carbohydrate was significant.

Yield and economics of moth bean [Vigna aconitifolia (Jacq.) Marechal] as influence by the levels of phosphorus, spacing and manures

Yield and economics of moth bean [Vigna aconitifolia (Jacq.) Marechal] as influence by the levels of phosphorus, spacing and manures

Spatio-Temporal Changes in Cropping Pattern in the State of Rajasthan, India

This study was carried out in the state of Rajasthan, India. Four major crop groups viz; cereals (pearlmillet, maize and wheat), pulses (moong bean, moth bean, kharif pulses and gram), oilseeds (soyabean and rapeseed and mustard) and others (cotton and clusterbean) were selected for analysis. All crops in the above groups with 5% or more area under cultivation in a district were chosen. The districts were selected on the basis of last five years average (2005-06 to 2009-10 Secondary data were collected from published records and reports of the Directorate of Economics and Statistics, Government of Rajasthan, Jaipur and official website of Rajasthan Agriculture Department www.krishi.rajasthan.gov.in.
 The study period encompassed a duration of 58 years starting from; 1956-57 to 2014-15. The data were also analyzed for twelve different periods by using Markov chain analysis technique for studying the change in cropping pattern.
 The study results revealed that the bajra crop was almost stable and cotton crop (except during-2000 to 2005) highly unstable with no retention of their previous area over the periods in the state of Rajasthan, but acreage of the crops was continuously shifting from one crop to another crop throughout the period. However, crops like kharif pulses, wheat, clusterbean, soyabean and mustard had more retention as compared to gram, cotton and maize during-1956- 57 to 2014-15 indicating its stability in the state.

Purification, molecular docking and in vivo analyses of novel angiotensin-converting enzyme inhibitory peptides from protein hydrolysate of moth bean (Vigna aconitifolia (Jacq.) Màrechal) seeds

The moth bean is a high-protein food legume. Enzymatic hydrolysates of food proteins demostrate health benefits. Search for diet related food protein hydrolysates is therefore within the scope of functional foods. Present study asertains to produce, screen and identify natural ACE-I inhibitory peptides derived from moth bean seed protein hydrolysates. The extracted protein was hydrolysed using alcalase, chymotrypsin, flavourzyme, papain, pepsin and trypsin respectively. Alcalase achieved the greatest degree of hydrolysis and ACE inhibition. The highest ACE-I inhibitory activity was exhibited by the peptide with the lowest molecular weight i.e. <3 kDa (IC50 11.19 ± 0.15 μg/mL). This was further separated by FPLC, followed by mass spectrometry. Molecular docking analysis showed the peptides IAWDFR and ADLPGLK bind to active sites whereas DKPWWPK and AVIPNAPNLR to non-active sites of the ACE molecule. In vivo administration of MBP hydrolysate to dexamethasone-induced hypertensive rats reduced their systolic blood pressure (125 ± 0.76 mmHg) compared with positive control (155 ± 3.13 mmHg). Moth bean protein peptides exhibit functional nutraceutical properties with adequate antihypertensive activity.

Comparative transcriptomic analysis reveals genes encoding polygalacturonase inhibitors and lectins as promising candidates conferring bruchid (Callosobruchus chinensis) resistance in moth bean (Vigna aconitifolia)

Comparative transcriptomic analysis reveals genes encoding polygalacturonase inhibitors and lectins as promising candidates conferring bruchid (Callosobruchus chinensis) resistance in moth bean (Vigna aconitifolia)

Studies on Pesticide Resistant Phosphate Solubilizing Bacteria from Grape Yard Soil

Dimethoate, a widely used organophosphate insecticide and acaricide to grape plants, is readily absorbed and distributed throughout plant tissues. The present work was based on isolation and studies on pesticide resistant phosphate solubilizing bacteria from grape yard soil. The Minimum inhibitory concentration [MIC] of normal garden microflora was observed to be 0.8% of dimethoate. The total of 6 pesticide resistant phosphate solubilizing bacteria [PP1, PP2, PP3, PP4, PP5, and PP6] were isolated from grape yard soil on Katznelson’s and Bose agar. Colony characteristics, morphological characteristics, motility, sporulation and capsulation of isolates were studied. Biochemical characteristics including carbohydrate utilization, H2S production and enzyme activities were studied. Agricultural importance of the isolates with respect to indole acetic acid production, nitrogen fixation, cellulose degradation, antagonistic effects on Azotobacter spp. and Rhizobium spp. were also studied. The pesticide tolerance was checked and seed germination test were performed. The effect of each isolate and mixed cultures on plant growth of moth bean seeds was studied. From the results, the isolate PP3 was found to be most important agriculturally producing indole acetic acid. The mixed culture also improved shoot and root growth. Further studies in this context are in process.

Identification and characterization of CAT1 gene during drought stress in moth bean [Vigna aconitifolia (Jacq.) Marechal

Moth bean [Vigna aconitifolia (Jacq.) Marechal] is a drought hardy orphan pulse crop. However, the information related to the identification and characterization of drought stress tolerance genes is very limited. Therefore, the present investigation was formulated to identified and characterized drought tolerant gene(s) from moth bean. Five genes were selected from available expression studies of moth bean and their expression pattern was assessed during time course experiment of drought stress in moth bean. During the time course experiment of drought stress in moth bean, the catalase1 (CAT1) gene was exponentially expressed in up-regulated manner. Thus, CAT1 gene of moth bean was identified as potential candidate gene and validated through Sanger’s sequencing. The genomic sequence CAT1 gene was named as VacoCAT1 and was further characterized using various bioinformatics tools. The VacoCAT1 showed an ORF with incomplete length of 213 bp which encoded 71 amino acids. The coding sequence of VacoCAT1 gene was shown a single exon due to incomplete nature of genomic sequences. The multiple sequence alignment of VacoCAT1 revealed the highly conserved region at 3’ site of the gene as compared to CAT1 gene of other crop species including legumes. The phylogenetic analysis of VacoCAT1 and CAT1 gene of other crop species including legumes revealed three clusters. The cluster VacoCAT1 gene showed close proximity with V. radiata CAT1 in cluster one of phylogenetic tree. The identified and characterized VacoCAT1 gene can be utilized as a genomic resource for enhance drought tolerance in susceptible pulses as well as other crops.

Development and Organoleptic Evaluation of Chakli Prepared from Green Gram Flour (Vignaradiata L. Wildzek.) and Moth Bean Flour (Vignaacontifolia)

The present study was undertaken with the objectives of evolving chakli containing green gram flour and moth bean flour to find out their acceptability and nutritive value. Chakli were prepared by using refined flour, green gram flour, moth bean flour, salt, red chilli powder and refined oil by substituting refined flour with green gram flour and moth bean flour. The different samples prepared were Control , Sample 1, Sample 2 and Sample 3 in the ratios of (refinedflour:green gram flour: moth bean flour) 100, 50:25:25, 50:45:5, 50:5:45 respectively. The developed chakli were sensory evaluated using nine point hedonic scale. Results showed that overall acceptability for Sample 3 (7.85±0.81) Chakli were lying in between the category of ‘like very much and like extremely whereas Control (7.6±1.53) were lying in the category of ‘like moderately and like very much’ by panelists.Highest energy, protein, carbohydrate and fat content were observed in Sample2 Chakli (520.8 kilocalories), (17.5 gram), (65.3 gram) and (22.2gram) respectively. Likewise fiber, calcium and iron content were observed in Sample 3 Chakli (2.4 gram) (108.6 milligram) and (5.84 milligram) respectively. Chakli (Sample 3) was most acceptable and analysed for proximate and mineral content along with control sample. Result shows that chakli prepared with green gram flour and moth bean flour (Sample 3) was found to be higher in protein (15.8 gram), fibre (1.9 gram), ash (2.5%), moisture (5.2%), calcium (19 milligram) and iron (1.1 milligram) than control chakli. Thus replacement of traditional food like refined flour with green gram flour and moth bean flour for preparing chakli is feasible and beneficial too and also were very accepted.

Unlocking the hidden variation from wild repository for accelerating genetic gain in legumes.

The fluctuating climates, rising human population, and deteriorating arable lands necessitate sustainable crops to fulfil global food requirements. In the countryside, legumes with intriguing but enigmatic nitrogen-fixing abilities and thriving in harsh climatic conditions promise future food security. However, breaking the yield plateau and achieving higher genetic gain are the unsolved problems of legume improvement. Present study gives emphasis on 15 important legume crops, i.e., chickpea, pigeonpea, soybean, groundnut, lentil, common bean, faba bean, cowpea, lupin, pea, green gram, back gram, horse gram, moth bean, rice bean, and some forage legumes. We have given an overview of the world and India's area, production, and productivity trends for all legume crops from 1961 to 2020. Our review article investigates the importance of gene pools and wild relatives in broadening the genetic base of legumes through pre-breeding and alien gene introgression. We have also discussed the importance of integrating genomics, phenomics, speed breeding, genetic engineering and genome editing tools in legume improvement programmes. Overall, legume breeding may undergo a paradigm shift once genomics and conventional breeding are integrated in the near future.

Vignette of Vigna domestication: From archives to genomics.

The genus Vigna comprises fast-growing, diploid legumes, cultivated in tropical and subtropical parts of the world. It comprises more than 200 species among which Vigna angularis, Vigna radiata, Vigna mungo, Vigna aconitifolia, Vigna umbellata, Vigna unguiculata, and Vigna vexillata are of enormous agronomic importance. Human selection along with natural variability within these species encompasses a vital source for developing new varieties. The present review convokes the early domestication history of Vigna species based on archeological pieces of evidence and domestication-related traits (DRTs) together with genetics of domestication. Traces of early domestication of Vigna have been evidenced to spread across several temperate and tropical regions of Africa, Eastern Asia, and few parts of Europe. Several DRTs of Vigna species, such as pod shattering, pod and seed size, dormancy, seed coat, seed color, maturity, and pod dehiscence, can clearly differentiate wild species from their domesticates. With the advancement in next-generation high-throughput sequencing techniques, exploration of genetic variability using recently released reference genomes along with de novo sequencing of Vigna species have provided a framework to perform genome-wide association and functional studies to figure out different genes related to DRTs. In this review, genes and quantitative trait loci (QTLs) related to DRTs of different Vigna species have also been summarized. Information provided in this review will enhance the in-depth understanding of the selective pressures that causes crop domestication along with nature of evolutionary selection made in unexplored Vigna species. Furthermore, correlated archeological and domestication-related genetic evidence will facilitate Vigna species to be considered as suitable model plants.

Moth Bean, Gelatin, and Murraya Koenigii Leaves Extract-Based Film and Coating: Effect of Coating on Shelf and Quality of Solanum Melongena

Moth bean starch (MS), gelatin (GA), and Murraya koenigii leaves extract (ME) are blended at different compositions to prepare film and coating according to casting and dipping approaches. Different MS, GA, and ME compositions were used to synthesize films and coating. The film compositions (MS : GA: ME: 60 : 20 : 20 and MS : GA: ME:20 : 60 : 20) were represented in terms of F3 and F4, respectively. The results showed that F3 exhibited better physicochemical properties than other films. In addition, SEM images showed that all components of the films were uniformly mixed and formed smooth surface morphology without cracks and bubbles. FTIR results indicate that ME in the films induces interactions between the film components, causing an improvement in compactness. Moreover, an optimized film-forming solution was tested as a coating. Parameters such as skin tightness, weight loss, pH, titratable acidity, and sensory analysis were considered to check the quality of coated Solanum melongena during storage. The results show that the formulation effectively maintains the quality parameters during storage. Furthermore, it also notices that coating extends the shelf life of Solanum melongena by one week.

DISEASES OF MOTH BEAN [VIGNA ACONITIFOLIA (JACQ.) MARECHAL

Pulses are obtained from leguminous plants that have many bioactive components and good nutritional value, being rich in protein, vitamins, and abundant macro- and micronutrients and thus contribute to global food security. Moth bean is compatible with the climatic conditions of Rajasthan because it is a drought-resistant crop though it is affected by many pathogenic microorganisms as fungi, bacteria, and viruses. Infection of these microorganisms causes damage to plants which results in loss of productivity. In the current context, a review on fungal, bacterial, and viral diseases of moth bean has been dealt with. The major diseases of moth bean reported are leaf blight, leaf spots, seedling stem and root rot, pod rot, web blight, common bacterial blight, and mung bean yellow mosaic virus (MYMV) causing yield loss.

​Effect of Inter and Sequential Cropping of Pulses in Little Millet (Panicum sumatranse L.) based Cropping System

Background: Small millets and pulses are world’s renowned crops for their nutritional significance. Being an underutilized species; rainfed farmers solely depend on them for their livelihood; which would be enhanced in a planned and efficient cropping system. The current study aimed to the identify suitable inter and sequential crops for rainfed little millet. Methods: In this study, four cropping patterns with two different row proportions for intercropping [viz., (4:1), (6:1)] were used with little millet as a main crop. The cropping systems include - little millet + pigeonpea [(4:1), (6:1)] followed by a sequential horsegram; little millet + pigeonpea followed by moth bean and little millet + lablab [(4:1), (6:1)] followed by Horsegram and little millet + lablab followed by mothbean. Result: Among them; a higher grain equivalent yield was observed in little millet + pigeonpea (6:1); followed by horsegram. However, higher dry matter, grain weight and higher individual crop yield was obtained in little millet + pigeonpea (6:1) followed by mothbean. Although individual mono cropping yields of these crops were higher; the pattern with little millet - pigeonpea (6:1) followed by horsegram could be recommended as a methodology to aid higher economic returns in shorter period.

Bionomics of Callosobruchus analis (F.) in ten common food legumes

Range of edible legumes suffer huge post-harvest deterioration due to infestation by Callosobruchus spp. (Coleoptera: Chrysomelidae). Among the bruchid species, despite wider distribution in India and its destructive potential, Callosobruchus analis (F.) has been largely overlooked. Hence, we report comparative bionomics of C. analis, influence of seed traits and seed losses in ten commonly cultivated food legumes namely, mungbean, urdbean, chickpea-desi, chickpea-kabuli, cowpea, field pea, pigeonpea, rajmash, moth bean and lentil. Oviposition behaviour, developmental rate of insect and seed losses varied significantly under the influence of seed physico-chemical traits under free-choice and no-choice conditions. Cowpea, pigeonpea and rajmash harboured higher number of eggs when choice for legume was offered, however, none of the tested legume was completely avoided for egg laying. The survivability was found negligible on rajmash and field pea. Developmental time was 1.3–1.5 times longer on urdbean (45.97 days) in comparison to other legumes, indicating the role of antibiosis factors. More progenies were produced on pigeonpea, cowpea, moth bean, chickpea-desi and mungbean leading to high seed losses. Seed properties namely, seed surface area, hardness, seed coat thickness, 100-seed weight, seed moisture and phenol contents significantly influenced oviposition behaviour and growth of the insects. Further studies are needed to elucidate exact mode of action of seed traits and their interaction on the insect bionomics. The legumes those suffered considerable seed losses by C. analis warrant adequate preventative measures to avoid cross-infestation under common storage conditions and reduce post-harvest deterioration.

Tandemly duplicated genes encoding polygalacturonase inhibitors are associated with bruchid (Callosobruchus chinensis) resistance in moth bean (Vigna aconitifolia)

Bruchids are stored-grain insect pests responsible for serious seed loss in legume crops. A previous study using an F2 population (F2OA) derived from a cross between wild moth-bean (Vigna aconitifolia [Jacq.] Maréchal) accession TN67 (resistant) and cultivated moth-bean accession ICPMO056 (susceptible) revealed that resistance to the azuki bean weevil (Callosobruchus chinensis L.) in TN67 was regulated by a single gene located in the major quantitative trait locus—qVacBrc2.1. In this study, qVacBrc2.1 was finely mapped and candidate genes in this locus were identified using F2OA and another large F2 population (F2NB) derived from the cross mentioned previously. In contrast to the previous study, segregation analysis in the F2NB population revealed that resistance against this pest was controlled by two genes. Furthermore, the addition of novel markers to qVacBrc2.1 and reanalysis of the QTL in the F2OA population demonstrated that qVacBrc2.1 constituted two linked QTLs—qVacBrc2.1-A and qVacBrc2.1-B. The presence of qVacBrc2.1-B was verified using the population F2NB. Comparative genomics using three Vigna spp. strongly suggested the presence of two tandemly duplicated genes, VacPGIP1 and VacPGIP2, which encoded polygalacturonase inhibitors (polygalacturonase-inhibiting proteins) as the candidates for conferring resistance, but only VacPGIP1 could be successfully cloned and sequenced. The alignment of VacPGIP1 coding sequences of TN67 and ICPMO056 revealed eight single nucleotide polymorphisms, three of which altered the amino-acid sequence of the predicted domains of polygalacturonase inhibitors in ICPMO056. Overall, these findings indicate that VacPGIP1 and VacPGIP2 regulated C. chinensis resistance in TN67.

Unraveling Origin, History, Genetics, and Strategies for Accelerated Domestication and Diversification of Food Legumes.

Domestication is a dynamic and ongoing process of transforming wild species into cultivated species by selecting desirable agricultural plant features to meet human needs such as taste, yield, storage, and cultivation practices. Human plant domestication began in the Fertile Crescent around 12,000 years ago and spread throughout the world, including China, Mesoamerica, the Andes and Near Oceania, Sub-Saharan Africa, and eastern North America. Indus valley civilizations have played a great role in the domestication of grain legumes. Crops, such as pigeon pea, black gram, green gram, lablab bean, moth bean, and horse gram, originated in the Indian subcontinent, and Neolithic archaeological records indicate that these crops were first domesticated by early civilizations in the region. The domestication and evolution of wild ancestors into today’s elite cultivars are important contributors to global food supply and agricultural crop improvement. In addition, food legumes contribute to food security by protecting human health and minimize climate change impacts. During the domestication process, legume crop species have undergone a severe genetic diversity loss, and only a very narrow range of variability is retained in the cultivars. Further reduction in genetic diversity occurred during seed dispersal and movement across the continents. In general, only a few traits, such as shattering resistance, seed dormancy loss, stem growth behavior, flowering–maturity period, and yield traits, have prominence in the domestication process across the species. Thus, identification and knowledge of domestication responsive loci were often useful in accelerating new species’ domestication. The genes and metabolic pathways responsible for the significant alterations that occurred as an outcome of domestication might aid in the quick domestication of novel crops. Further, recent advances in “omics” sciences, gene-editing technologies, and functional analysis will accelerate the domestication and crop improvement of new crop species without losing much genetic diversity. In this review, we have discussed about the origin, center of diversity, and seed movement of major food legumes, which will be useful in the exploration and utilization of genetic diversity in crop improvement. Further, we have discussed about the major genes/QTLs associated with the domestication syndrome in pulse crops and the future strategies to improve the food legume crops.

The Effect of Paddy and Coffee Husk as the Organic Manure on the Vegetative and Yield Parameters of Moth Bean [Vigna aconitifolia (Jacq) Marechal

Background: Amidst of emerging world with the new technologies we are in the dangerous condition regarding the disposal of agro industrial wastes which creates a serious problem. Organic manures can prevent and can enhance the productivity in the plants as well as can reduce the environmental issues. Methods: The study was conducted in the month of September to November 2019 at St. Joseph’s College Campus, Visakhapatnam, A.P. with the bio composting process of paddy and coffee husk along with Pleurotus eous, Pleurotus florida, Trichoderma asperelloides and Eisenia fetida, with 6 treatments. The plants were analyzed for root length, shoot length, number of leaves, number of nodules, fresh weight and dry weight of the plant on 20, 40 and 60 DAS and the yield parameters were analyzed on 90th day of the plant. Result: The results showed a great increase in the treatment 6 (Pre decomposed coffee husk, consortium of Pleurotus eous, P. florida, Trichoderma asperelloides and Eisenia fetida) in root length (8.5, 12.9 and 16.1 cm) shoot length (15.2, 30.3 and 43.2 cm) number of leaves (11, 18 and 49) number of nodules (12, 20 and 9) fresh weight (4.83, 8.98 and 15.47 g) and dry weight (1.11, 3.56 and 4.37 g) when compared with the other treatments and control. The yield parameters also showed significant results in number of pods (27), length of the pod (5.7 cm), number of seeds/pod (6), weight of the seeds/pod (2.89 g), fresh weight of the pod (1.653 g) and dry weight of the pod (0.986 g).

Identification of 'Start of Season' in Major Rainfed Crops of Tamil Nadu, India Using Remote Sensing Technology

Cropping seasons are the seasons in which a particular crop is grown. Identification of cropping seasons is crucial for successful cropping in rainfed areas. This research study focuses on the identification of major rainfed crop growing seasons in rainfed areas of Tamil Nadu using remote sensing technology. The MODIS (Moderate-Resolution Image Spectroradiometer) satellite product MOD13Q1 is utilized to produce NDVI (Normalized Difference Vegetation Index) of the study region. Then the NDVI values for each rainfed ground truth point were extracted and the value was used to produce a line graph representing the crop growing period. Likewise, multiple lines were produced for major rainfed crops: Rice, Maize, Pearl millet, Sorghum, Groundnut, Moth bean, Cotton and Chilli. The result reveals that the Maize and Cotton growing season starts in June-July, mostly single crop per year. Rice, Pearl millet, Sorghum and Chilli are also grown as a single crop per year, sowing by the month of October-November. Besides, there was a double-cropping area of Groundnut- Black gram and Groundnut-Moth Bean raised during June-July and October-November respectively.

Isolation and Screening of Efficient Rhizobial Strains and Evaluation of Their Efficiency in Moth Bean (Vigna aconitifolia Jacq)

Background: Moth bean (Vigna aconitifolia Jacq.) is a legume possess a higher nutritional value with 22–24 per cent protein and also used as a pasture legume. Fertilizer applications to moth bean are uncommon in India and mostly grown with minimum inputs and care. The main objective is to increase the production and productivity in moth bean crop, using crop or location specific, native rhizobial strains. Methods: In vitro studies on isolation and characterization of Rhizobium from moth bean root nodules was carried out and further its efficiency was tested under pot culture conditions. Result: Among the thirty isolates obtained, 11 showed positive reactions for poor growth on glucose peptone agar medium, Keto-lactose and catalase activities, citrate utilization in in vitro studies and were tentatively identified as Rhizobium. By 16S rRNA sequencing and culture number MB-1 was identified as Rhizobium skierniewicense. Biometric observations revealed that MB-1 inoculated plants significantly recorded the highest plant height of 34 cm; root length of 14 cm; 24 numbers of leaves per plant having the maximum nodule number of 27 and maximum number of pods of 39 pods plant-1 and its corresponding single plant yield of seeds 18.6 g followed by COG15 inoculated plants which recorded 15.5 g of seeds in a plant. The native isolates Rhizobium skierniewicense, (MB-1) proven to be a effective strain resulted in higher and bigger nodules, increased biometrics and yield attributes.