Mosquito Borne Research Articles

Study of Mosquito Borne Deasease Found in Yavatmal Region

Abstract: Mosquito-borne diseases are a significant public health concern in the Yavatmal region of Maharashtra, India. Malaria, dengue fever, and Japanese encephalitis are among the prominent diseases transmitted by mosquitoes in this region. Malaria, caused by the Plasmodium parasite, is endemic in Yavatmal, with transmission occurring mainly during the monsoon and post-monsoon seasons. Dengue fever, caused by the dengue virus and transmitted by Aedes mosquitoes, has seen periodic outbreaks in the region, with increased incidence during the rainy season. Japanese encephalitis, caused by the Japanese encephalitis virus and transmitted by Culex mosquitoes, poses a threat to the population, particularly in rural areas. Several factors contribute to the spread of mosquito-borne diseases in the Yavatmal region, including inadequate sanitation, stagnant water bodies, deforestation, and climate change. Socioeconomic factors such as poverty and limited access to healthcare further exacerbate the problem. Control and prevention efforts in the region primarily focus on vector control measures, including insecticide spraying, larval source management, and the use of bed nets. Community education and awareness campaigns play a crucial role in promoting preventive behaviors such as the use of insect repellents and proper waste disposal. Challenges in addressing mosquito-borne diseases in Yavatmal include inadequate healthcare infrastructure, insufficient funding, and the emergence of insecticide resistance among mosquito populations. Collaborative efforts involving government agencies, healthcare providers, researchers, and community organizations are essential to effectively combat these diseases. Strengthening surveillance systems, improving access to diagnostic and treatment facilities, and implementing integrated vector management strategies are necessary steps towards reducing the burden of mosquito-borne diseases in the Yavatmal region and improving public health outcomes.

Commonly Reported Mosquito-Borne Viruses in the United States: A Primer for Pharmacists.

Mosquito-borne diseases are a public health concern. Pharmacists are often a patient's first stop for health information and may be asked questions regarding transmission, symptoms, and treatment of mosquito borne viruses (MBVs). The objective of this paper is to review transmission, geographic location, symptoms, diagnosis and treatment of MBVs. We discuss the following viruses with cases in the US in recent years: Dengue, West Nile, Chikungunya, LaCrosse Encephalitis, Eastern Equine Encephalitis Virus, and Zika. Prevention, including vaccines, and the impact of climate change are also discussed.

Knowledge, attitude and practices regarding dengue and its vector among medical practitioners in Malakand region, Pakistan.

Dengue fever (DF) is increasingly recognized as one of the world's major mosquito borne diseases and causes significant morbidity and mortality in tropical and subtropical countries. Dengue fever is endemic in most part of Pakistan and continues to be a public health concern. Knowledge, attitude and practices can play an important role in management of the disease. Current study was aimed to determine the level of knowledge, attitude and practices regarding dengue fever among health practitioners, to study the level of knowledge and attitude with preventive practices for dengue fever. A cross sectional study was carried out in medical practitioners of the four districts of Malakand region during October to November 2019. A pre-structured questionnaire was used to collect data from medical practitioners. Data was analyzed using Graph Pad version 5. Significant value was considered when less than 0.05 (at 95% confidence of interval). The results revealed that most of participants have seen dengue vector (62%), the media being the most quoted source of information. Nearly 81.2% participants were aware from transmission of dengue fever is by mosquito bite. Practices based upon preventive measures were found to be predominantly focused towards prevention of mosquito bites rather than elimination of breeding places. Although the knowledge regarding DF and mosquito control measure was quite high among the medical practitioners but this knowledge was not put into practice. Further studies are required to aware the people about dengue and its vector in order to get prevention and control.

A Study on Mosquito Density and Trend of Larval Indices from a Hospital Campus of Rural Area, Thrissur District Kerala

Introduction: Dengue is one of the most important arboviral disease in Kerala. Development of the control measures mainly depends upon the knowledge regarding the bionomics of mosquito. The larval indices are one of the methods of entomological surveillance, which can predict the outbreak of mosquito borne diseases. The hospital premises need to be mosquito free and need to be safe place for staff and patients. Hence, the present study was done to calculate the standardized larval indices, trends and to determine major breeding source in hospital campus of, Thrissur district, Kerala.

Effect of hot water leaf and bark extract of Swietenia mahagoni against the Culex quinquefasciatus and Aedes aegypti larvae

Mosquito larvae control is essential to prevent mosquito borne diseases such as dengue, Zika, filariasis and chikungunya. Plant extract has the larvicidal properties with less toxicity to other living creatures and no resistance compared to synthetic chemical pesticides. Effect of hot water leaf and bark extract of a forest tree species (Swietenia mahagoni) against the 3rd instar larvae of Culex quinquefasciatus and Aedes aegypti was studied in vitro using five different concentrations (1-5%) of hot water leaf and bark extracts of Swietenia mahagoni. It was observed that, 5% concentrated hot water leaf extract was the most toxic killing 70% larvae of Culex quiquefasciatus and 50% larvae of Aedesa egypti after 24 hours exposure. Similarly, 5% hot water bark extracts killed 65% and 55% of Culex quiquefasciatus and Aedes aegypti larvae respectively. The minimum LC50, LC90 and LC95 values of hot water leaf extract of Swietenia mahagoni against Culex quinquefasciatus Say and Aedes aegypti (L.) with 95% confidence limits were 2.662, 6.968, 8.70 and 2.128, 4.964, 6.054 respectively after 72 hours of exposure. In hot water bark extract of Swietenia mahagoni against Culex quinquefasciatus Say and Aedes aegypti (L.) with 95% confidence limits after 72 hours of exposure minimum LC50, LC90 and LC95values were 1.780, 6.540, 9.458 and 1.370, 4.570, 6.430. The present investigation revealed the possible utilization of hot water leaf and bark extract of Swietenia mahagoni to control mosquito borne disease. Further study is needed to know the active ingredients of the leaf and bark extract of the experimented plant. Asian J. Med. Biol. Res. 2022, 8 (2), 94-102

Knowledge, attitude, and practice regarding mosquito-borne diseases among migrant laborers from a migrant settlement in Ponekkara, Ernakulam Kerala.

Mosquito-borne diseases (MBDs) such as Malaria, Dengue, Chikungunya, lymphatic filariasis, and Japanese Encephalitis are important public health problems in India. Ernakulam in Kerala being a hub of construction activities has a large influx of migrants from Odisha, West Bengal, Bihar, Assam, U. P., Jharkhand, T. N., and Karnataka. Hence, the objective of this study was to assess the knowledge, attitude, and practice related to MBDs and the associated factors among the migrant laborers from a migrant settlement in Ponekkara, Ernakulam Kerala. A cross-sectional study was done among 179 migrant laborers from a migrant settlement in Ponekkara, Kerala, from September 2021 to November 2021 using a pretested semi-structured questionnaire to collect information regarding socio-demographic details and their knowledge, attitude, and practice regarding mosquito borne diseases. After taking verbal consent, the questionnaire was administered by the investigator. Descriptive and univariate analysis was done using SPSS Version 20. It was found that 58.4% of the migrant laborers had poor knowledge, 55.9% had poor attitude, and 61.5% of them had poor practice regarding MBDs. On univariate analysis, a statistically significant association was observed between attitude score and the level of education. The findings showed that migrant laborers had an overall poor knowledge, attitude, and practices regarding MBDs. Consequently, there is a need to plan an awareness program among the migrant settlements regarding MBDs.

Culex Mosquitoes at Stormwater Control Measures and Combined Sewer Overflow Outfalls after Heavy Rainfall

Mosquito borne diseases are increasingly problematic as climate change continues to alter patterns of precipitation, flooding, and temperatures that may favor mosquito habitats. Stormwater control measures (SCMs), ecologically sustainable methods of stormwater management, may have varying impacts on Culex mosquitoes, such as in areas with combined sewer overflows (CSOs). We studied spatial and temporal associations of SCMs and Culex mosquito counts surrounding the SCMs, stratifying our examination amongst those that do/do not use pooling and/or vegetation, as well as surrounding CSO outfalls after heavy rainfall (≥95th percentile) during summer 2018. Results indicate Culex mosquito counts after heavy rainfall were not significantly different at SCMs that use vegetation and/or ponding from at those that do not. We also found a 35.5% reduction in the increase of Culex mosquitoes the day of, and 77.0% reduction 7–8 days after, heavy rainfall at CSO outfalls treated with medium SCM density compared to those without SCMs. Our results suggest that SCMs may be associated with a reduction in the increase of Culex mosquitoes at the CSO outfalls after heavy rainfall. More research is needed to study how the impacts of SCMs on mosquito populations may affect human health.

Use of intravenous N-acetylcysteine in acute severe hepatitis due to severe dengue infection: a case series

BackgroundDengue fever is a common mosquito borne viral infection. Severe dengue fever associated severe hepatitis carries high mortality. Based on the beneficial effect of N-acetylcysteine (NAC) in paracetamol poisoning and non-acetaminophen induced liver failure, it is used in dengue fever associated hepatitis in clinical practice. We aim to study the reversal of liver enzymes with NAC in the setting of severe hepatitis due to severe dengue infection.MethodsA retrospective analysis was conducted on hospitalized 30 adults with severe dengue fever with severe hepatitis. These 30 patients had aspartate transaminase (AST) and alanine transaminases (ALT) more than 500 U/L and/or PT INR (prothrombin time and international normalized ratio) more than 1.5. They were treated with NAC infusion of 100 mg/h for 3 to 5 days.ResultsThe mean age of the group was 49.9 ± 11.46 years and 18 (60%) patients were males. Nineteen patients (63%) developed dengue shock. Of them 12 patients (40%) developed hepatic encephalopathy. Median AST on the day of administration of NAC was 1125 U/L interquartile range (IQR) 1653.25 while median ALT was 752 (IQR 459.25). There was a statistically significant reduction of both ALT (p = 0.034) and AST (p = 0.049) from day 1 to 4 after NAC infusion. Rise of platelet count between day 1 and day 4 also showed statistically significant difference (p = 0.011) but the reduction of prothrombin time and international normalized ratio (PT/INR) from 1 to day 4 did not show statistical significance difference. Mean duration of treatment with NAC was 3.61 ± 0.75 days while mean length of hospital stay was 6.2 ± 1.27 days. Only one patient died (3.3%). None of the patients reported adverse drug reaction due to NAC.ConclusionMajority of patients demonstrated marked clinical and biochemical improvements and they recovered fully. We observed faster and significant recovery of liver enzymes following administration of NAC. Based on the above findings, this study provides preliminary evidence for the beneficial effect of NAC in severe hepatitis in dengue infection with greater survival benefits.

Dengue Infection: Frequently Asked Questions by People in the Province of Aklan, Philippines

Dengue virus is the most common mosquito borne viral disease in humans, and poses a major challenge to global public health services. Infection can be caused by any of the 4 DENV serotypes, transmitted by female Aedes aegypti mosquitoes. Presenting features may vary from a mild self-limiting febrile illness to life-threatening symptoms of bleeding, organ impairment, and plasma leakage leading to shock. Early diagnosis and monitoring are critical to reduce mortality, especially in the context of the COVID-19 pandemic. Laboratory tests, such as the serological detection of either antigen or antibodies are useful in the diagnosis. Currently, although a vaccine for DENV is available, it remains a challenge to develop an effective vaccine against 4 discrete serotypes and antiviral drugs effective in reducing morbidity or improving disease outcome.

Mosquito species identification using convolutional neural networks with a multitiered ensemble model for novel species detection

With over 3500 mosquito species described, accurate species identification of the few implicated in disease transmission is critical to mosquito borne disease mitigation. Yet this task is hindered by limited global taxonomic expertise and specimen damage consistent across common capture methods. Convolutional neural networks (CNNs) are promising with limited sets of species, but image database requirements restrict practical implementation. Using an image database of 2696 specimens from 67 mosquito species, we address the practical open-set problem with a detection algorithm for novel species. Closed-set classification of 16 known species achieved 97.04 ± 0.87% accuracy independently, and 89.07 ± 5.58% when cascaded with novelty detection. Closed-set classification of 39 species produces a macro F1-score of 86.07 ± 1.81%. This demonstrates an accurate, scalable, and practical computer vision solution to identify wild-caught mosquitoes for implementation in biosurveillance and targeted vector control programs, without the need for extensive image database development for each new target region.

Management of dengue with co-infections: an updated narrative review.

Dengue is a life-threatening mosquito borne viral disease. We are still in the era of supportive treatment where morbidity and mortality are a major concern. Dengue infection in presence of other co-infections makes this scenario rather worse. Timely recognition and raising alarm to be intensive is the need of the hour for primary care physicians practicing in the community and indoors. This review provides a comprehensive knowledge about the recent trends of coinfection in dengue as well as their management consideration which will be particularly helpful for physicians practicing in rural and remote areas of India.

English

BACKGROUND Dengue is a major public health concern in tropical Asian countries and a most rapidly spreading mosquito borne viral disease with a 30 fold increase in global incidence over the last five decades. Dengue is among the ten leading causes of death in children ranging from 1 to 15 years of age in tropical Asian countries. Complications of dengue fever are common in children. But very limited studies are available on paediatric populations. The virus or non-structural protein (NS1) interacts with platelets and coagulation factors. A combined effect of mild disseminated intravascular coagulation, liver damage and platelet dysfunction results in bleeding in dengue fever. With this scientific knowledge background, the present study was undertaken to identify and document the changing pattern of dengue infection in paediatric age group with a special emphasis on laboratory findings of dengue cases in paediatric age group. METHODS The present descriptive study was conducted for two years in a state run paediatric referral hospital. The sample was purposive by design and consisted of patients who have been diagnosed as having dengue fever with warning signs and admitted. RESULTS Fever was typically high grade (> 103 F) and most patients presented with warning signs within 5 days of illness (64.2 % in year 2016 and 76.6 % in year 2017). Persistent vomiting and abdominal pain were the most common warning signs in both the years. Thrombocytopenia was the commonest laboratory finding. Bleeding in study subjects lead to < 50000 / cu mm platelet count. Most common bleeding was gastrointestinal bleeding (39.2 % of study subjects with platelet count < 50000 / cu mm presented with G. I bleeding). CONCLUSIONS Dengue illness comes with varied presentation. Atypical presentations can delay the diagnosis. Early recognition of warning signs keeping the atypical presentation in mind is important. KEYWORDS Dengue Fever, Paediatric Age Group, Non-Structural Protein (NS1), Thrombocytopenia

How Flower Power Helped Save Millions of Lives

How the development of synthetic pyrethroids from natural pyrethrum led to new insecticides with high efficacy against the vectors of mosquito borne diseases such as malaria, dengue, yellow fever, Zika, elephantiasis, West Nile virus and chikungunya with opportunities for use in crop protection an added bonus.

Pattern of Mosquito Borne Parasitic Infection in the Night Blood Samples of Patients with Elevated TNF-α of> 5.0 pg/ml

Study Background: Plasmodium spp., (Protozoan) and Wuchereria bancrofti (Nematode) are transmitted by mosquitos to cause insect borne diseases known as malaria and Lymphatic filariasis/Elephantiasis. Apart from the social implication of these parasitic infections the infections can also elicit immune responses. Aim and Objective: This work was therefore designed to determine the pattern of mosquito borne parasitic infection in the night blood samples of patients with elevated TNF-α of > 5.0 pg/ml. Materials and Methods: Seventy (70; aged 31 – 76 years; Male- 35; Female-35) volunteers with plasma TNFα of 5.8 ±0.7 pg/including age-matched control participants ( n= 50 ; TNFα of 2.2 ± 0.3 pg/ml). All participants were negative to Acid Fast Bacilli, ant-HCV, HBsAg and HIV tests were recruited for the study. Night blood samples and sputum samples were obtained from the participants. Blood sample was used for determination of TNFα, HIVp24ag-Ab, anti-HCV, HBsAg by ELISA and identification of Plasmodium and Wuchereria by Geimsha thick blood film staining while sputum samples were used for the demonstration of Acid Fast Bacilli by Ziehl Neelsen staining. Results: The results showed a frequency of Plasmodium spp., in individuals with plasma TNF-α of 5.8 ±0.7 pg/ml as 31.4%(22) as against a frequency of 18%(9) in subjects with plasma TNF-α of 2.2 ± 0.3 pg/ml.. The results also showed a frequency of 5.71%(4) and2%(1) Wuchereria bancrofti in subjects with plasma TNF-α of 5.8 ±0.7 pg/ml and TNF-α of 2.2 ± 0.3 pg/ml. respectively. The overall frequency of parasitic infection obtained in both test and control subjects include: 33.3% (40)Plasmodium spp., and 4.2%(5) Wuchereria bancrofti.The overall results from both test and control subjects also showed a gender distribution of 20%(24) and 13.3%(16) Plasmodium spp.,in female and males respectively while a distribution of 1.7%(2) and 2.5%(3) Wuchereria bancrofti in females and males respectively. Conclusion: This work revealed increase in the frequency of Plasmodium spp. and Wuchereria bancrofti infections with increase in plasma TNF-α while the overall frequency of parasitic infection obtained in both test and control subjects was found to be 33.3% (40)Plasmodium spp., and 4.2%(5) Wuchereria bancrofti with possible variations in regional and gender distributions. Mosquito borne parasitic infection of Plasmodium spp., was found to be more prevalent in patients with elevated TNF-α of> 5.0 pg/ml.

Natural Anophelin mosquito repellents and phytochemical analysis of ethanol and hexane leaf extracts from four plant species

Currently mosquitoes control is the cornerstone to minimize the rising number of mosquito borne diseases. There is an urgent need looking for alternatives to the current reliance on synthetic insecticides for the vectors control. Application of active toxic agents from plant extracts as an alternative control strategy was available from ancient times. These are non-toxic, easily available at affordable prices, biodegradable and show broad-spectrum target-specific activities against different species of vectors. Natural products with repellency properties are urgently needed. An insect repellent works by masking human scent, and a number of natural and synthetic mosquito repellents were studied. The main aim of this study was to identify the phytochemicals and compare their potentials as mosquito repellents from the ethanol (Et. OH) and hexane (hex) leaf extracts (LEs) of Ocimum basilicum, Coleus forskohlii, Eucalyptus camaldulensis and Cymbopogon flexuosus under laboratory conditions at 50 and 100% concentrations. Phytochemical analysis showed that alkaloids, saponins, flavonoids, terpenes, tannins and terpenoids were present/or absent in the 4 plant-LEs. At 50% concentration of Et. OH extracts, C. forskohlii exhibited higher repellency potential on Anopheles gambiae with protection time of 137.3 min, while O. basilicum, E. camaldulensis and Cy. flexuosus registered protection time of 30.6, 15.3 and 19 min, respectively. At 100% concentration of ethanol- extracts, C. forskohlii caused the highest protection time against the vector with protection time of 182 min. O. basilicum, E. camaldulensis and Cy. flexuosus were equally less potent against the insect, with protection time 42.6, 32.6 and 28 min, respectively. Regarding hex-extract, at 50% concentration of C. forskohlii, the highest repellency potential , with protection time of 174 was registered, while O. basilicum, E. camaldulensis and Cy. flexuosus registered protection times of 44, 18 and 28.6 min, respectively. However, the 100% concentration of Et. OH-extracts of, C. forskohlii and O. basilicum exhibited the highest protection time, with protection times of 228 and 116min, respectively. E. camaldulensis and Cy. flexuosus were less potent, with protection times 28.6 and 54.6 min, respectively. The EOs of C. forskohlii and O. basilicum L EOs proved to have potentials as repellency agents against Anopheles. GC-MS analysis of the extracts identified the compounds of both solvents extracts and the terpens were the major compounds. According to the results, further studies are required, especially on the extracts of basil and coleus since they proved to be effective.

Community Perception Regarding Mosquito-Borne Diseases in Some Selected Areas of Ganjam District of Odisha State, India.

Mosquito-borne diseases are a major public health concern. Because of absence of effective therapy and vaccination to treat and prevent such vector-borne diseases, these initial cases have triggered strong concerns within medical communities, turning a bad dream into reality. To date, reducing the mosquito vector population remains the main strategy to control the transmission of mosquito borne diseases. In this connection, community-based perception recognized as an essential tool for long-term vector control management. In this study, a community perception survey was carried out in six selected study areas of Ganjam district of Odisha. A total of 3257 inhabitants from randomly chosen 600 no. of houses from 06 selected study areas were interviewed using a pre-tested structured questionnaire about various mosquito vector-borne diseases. As an outcome, we found that only 36% of people have basic knowledge and idea about vector-borne diseases, out of which only 14% know Anopheles as a causative agent whereas Aedes and Culex were very least concern. Regarding the source of knowledge, electronic media was the principal source of information, while the involvements of health personnel were least mentioned. Results of the current study indicates the necessity to plan health education program to bring important perceptions regarding vector-borne diseases at school and college level because the infrequent source of knowledge is the main cause of an increase in the disease incidence.

Co-infection of a mosquito borne arbovirus and parasite in dogs in Kaduna and Zaria metropolis, Kaduna state, Nigeria: A Case Report

During a survey in Kaduna and Zaria metropolis for West Nile virus and Dirofilaria in dogs, a single case of co-infection of the two organisms was observed in a dog. These organisms are both mosquito borne and are of great zoonotic importance.Running title: Co-infection of West Nile virus and Dirofilaria in dogs.
 Keywords: West Nile virus, dogs, Dirofilaria, co-infection

Species composition and land use pattern of mosquito vector species in Sonitpur district, Assam

The risk of mosquito-borne diseases has put world under threat for long years. Many dependant variables, particularly physical and biological parameters of the breeding site directly influence the species richness and abundance patterns of mosquito species. The present study is an attempt to map the mosquito vector distribution in Sonitpur district of Assam, an endemic district in terms of mosquito borne diseases. Mosquito vectors are collected using CDC light traps from 21 different habitats along with their geographic coordinates. Geospatial technologies have been used for better interpretation of the mosquito distribution pattern. This study reveals that, the Anopheles and Culex mosquitoes are the dominant species. Malaria vectors are dominant in the northern part of the district due to the high extents of forest patches. Culex species is mainly distributed in the wetlands associated area and Mansonia species does not show affinity towards a particular habitat. Result of the study will be a significant endeavour in improving the scientific understanding and effective controlling the mosquito-borne diseases.

DEVELOPMENT OF NANOEMULSION AND ITS CHARACTERIZATION FOR ANTI BIOFILM AND LARVICIDAL ACTIVITY

Dengue is a mosquito borne viral disease and the main root cause vector is Aedes aegypti. Hence in this work a rapid measure was taken to eradicate the vector using nanoemulsion. Plant essential oil based nanoemulsions was formulated by ultrasonic emulsification method. In the current study, nanoemulsion (O/W) was developed using oil extracted from the leaves of a Tulsi, Neem and Nochi. The emulsions were prepared in different ratios of oil and surfactant (Tween 80) and evaluated for their mechanical and thermal stability. After the confirmation of stable nanoemulsion formation, it was evaluated for its antibiofilm activity. Results revealed that the formulated mixed oil nanoemulsion exhibited enhanced antibiofilm activity against isolated microorganisms from contaminated wall samples. Further the biofilm forming capability of bacterial culture was indirectly assessed by staining with 1% crystal violet and measured absorbance using ethanol as a destaining solution. The mean biofilm production of S3 bacterial isolates significantly greater than that observed for S1 & S2. In addition, antibiofilm activity of the emulsion NE3 was higher against bacterial culture S3. Different concentration of mixed oil nanoemulsiom (NE3) treated group was subjected to evaluate the larvicidal activity against Aedes aegypti between 1 to 12 hours respectively. It was observed that an increase in the nanoemulsion concentration (NE3) to 25 ppm and 50 ppm resulted in complete loss of larval viability in 5 hr and 6 hr respectively. There is no significant difference in larval viability after 6 hr and 12 hr in all the treatment groups. Hence the above findings suggest the applicative potential of mixed nanoemulsion formulation for various applications including mosquito larval control and antibiofilm activity.

A systematic study to identify the awareness, severity and burden of mosquito-borne diseases in Maharashtra, India

Background: Mosquito-borne diseases are a rising concern. There are six mosquito-borne diseases in Maharashtra, the Indian state, which include Malaria, Dengue, Chikungunya, Japanese Encephalitis, West Nile Virus, and Lymphatic Filariasis. The status and trends of the five malaria, dengue, chikungunya, yellow fever and Zika diseases was reviewed in this study. The world's mosquito species and India's mosquito species were studied. The goal of this review is to identify awareness, seriousness and to find burden of mosquito-borne diseases in Maharashtra from 2016 to 2020. Materials and Methods: The present study was conducted in Maharashtra's urban cities.We carried out a survey using a questionnaire to assess the real burden of mosquito-borne diseases, and we also collected data to validate our findings from the National Vector Borne Diseases Control Program Directorate (NVBDCP) in Delhi. Results: We have got the 38% respondent suffered from Malaria, 18% suffered from Dengue, 31% suffered from Chikungunya, 4.1% suffered from Yellow Fever. It was also found that 60% of respondent were not suffered from any mosquito-borne disease in Maharashtra, India. We found that 64% people need hospitalization to cure mosquito borne diseases. Conclusion: To promote better awareness and to be conscious of mosquito-borne diseases, steps must be taken. The burden of malaria is higher than all other diseases transmitted by mosquitoes. Dengue patients are also found to be less than Chikungunya. There are records of death in Malaria and Dengue, but there are no recorded cases of death in Chikungunya.