Morus Alba Research Articles

Microclimatic effects of introduced tree plantings in urban environment

Ecological role of urban green spaces is increasing in order to provide citizens with comfortable and safe living conditions in the new climatic realities through the use of ecosystem services of tree and shrub stands in the setting of reduced forest areas, native forest decline and acceleration of urbanization processes. However, in Europe and, in particular, in Ukraine, deterioration of the environmental condition and loss of ecological functions of urban green spaces are observed. One of the reasons for the loss of ecological potential of tree and shrub plantings is unbalanced approach to the development of green infrastructure in cities, when socio-cultural ecosystem services (decorative, aesthetic) are preferred over environmental and regulatory ones, including over climate-regulating services. The active use of introduced tree species has always been driven by the development of green infrastructure in modern cities; the share of these species in dendroflora of urban ecosystems is steadily increasing in Europe. Introduced tree species comprise the basis of urban green infrastructure; therefore, the issue of determining the potential microclimatic effects of these species becomes relevant. The influence of tree species (Acer pseudoplatanus, Fraxinus pennsylvanica, Gleditsia triacanthos, Morus alba, Quercus rubra, Robinia pseudoacacia, Tilia platyphyllos) introduced in the Steppe zone of Ukraine on the formation of microclimatic parameters (light intensity, temperature and relative air humidity) in the space beneath the tree canopy within monodominant group plantings is analyzed in Botanical Garden of Oles Honchar Dnipro National University and park areas of Dnipro city. Beneficial effect of group plantings consisting of introduced tree species on the processes of optimizing the microclimatic conditions of the urban environment is revealed. Beneath the canopy of introduced tree species (compared to the reference open sites), light intensity decreased from 86.9% to 97.9% at midday in late July. The air temperature decreased from 3°C to 6.5°C, and the relative humidity increased by 2.5–9.8%, depending on the species of the tree. By increasing the microclimatic efficiency, the studied tree introducents were determined to form the following series: Gleditsia triacanthos – Robinia pseudoacacia – Fraxinus pennsylvanica – Quercus rubra – Tilia platyphyllos – Acer pseudoplatanus – Morus alba. Along with a significant climate-regulating effect, monodominant group plantings consisting of dense- and semi-dense crowned introduced tree species become "cooling islands" and play a significant role in optimizing the microclimatic conditions of urban ecosystems during the daytime and summer periods. At the same time, introduced tree species with naturally developed dense and semi-dense crowns can be prioritized for creating and reconstructing green spaces with recreational purposes in the cities situated in Steppe zone. The results obtained regarding the microclimatic effects of introduced tree species expand the scientific and theoretical base for the development of methods for optimizing the functionality of green infrastructure in cities by the construction of green spaces using tree species that have significant climate-regulating properties.

Genome-Wide Characterization of the Glycosyltransferase Family in Morus alba L. and Functional Characterization of the Flavonoid-Specific MaUGT89AS1

Genome-Wide Characterization of the Glycosyltransferase Family in Morus alba L. and Functional Characterization of the Flavonoid-Specific MaUGT89AS1

Pharmacological Properties of White Mulberry (Morus alba L.) Leaves: Suppressing Migratory and Invasive Activities Against A549 Lung Cancer Cells.

Morus alba known as a white mulberry is a medicinal plant that has been used in food ingredients and traditional medicine. M. alba leaves contain various bioactive phenolic compounds, in particular chlorogenic acid (CGA), which is a major bioactive ingredient. Their anticancer potency of M. alba leaf extracts derived from Soxhlet extraction was evaluated based on cytotoxicity and antimigratory and antiinvasive properties. The dichloromethane extract exhibited the highest nitric oxide radical scavenging activity with a half-maximal inhibitory concentration (IC50) value of 780μg/mL, promising cytotoxicity against HuCCA-1, MCF-7, and A-549 cells with IC50 values of 59.18, 62.20, and 103.25μg/mL, respectively. CGA selectively inhibited the growth of MCF-7 cells with an IC50 value of 26.75μg/mL and showed potent radical scavenging activity against DPPH radicals (IC50 = 18.85μg/mL). An ethanolic extract derived from the gradient Soxhlet extraction suppressed A549 lung cancer cell migration and invasion more effectively than CGA with no migratory inhibition effect on noncancerous HaCaT cells. Furthermore, the ethanolic extract and CGA accelerated HaCaT wound closure at 20µg/mL, which was the same as allantoin. Bioactive ingredients including triterpenes, steroids, phenolics, and flavonoids were mainly detected in all extracts. The highest content of CGA (52.23g/100g dry weight) was found in the ethanolic extract derived from the gradient Soxhlet extraction. These findings show the potency of the dichloromethane extract as a cytotoxic agent against various cancer types and the ethanolic extract as an antimetastatic agent by their antimigratory and antiinvasive activities.

Preharvest calcium chloride sprays improving physicochemical quality of mulberries (Morus alba L. ‘Chiang Mai 60’) during refrigeration

Preharvest calcium chloride sprays improving physicochemical quality of mulberries (<i>Morus alba</i> L. ‘Chiang Mai 60’) during refrigeration

Characteristic of species Morus alba L. and Morus nigra L. by some basic vegetative traits

Characteristic of species Morus alba L. and Morus nigra L. by some basic vegetative traits

Synthesis and study of the processes of biochemical action of the coordination compound of cobalate II-nitrate with quinazolin-4-one, 6-benzylaminopurine on the Morus alba variety of Moraceae plants

Synthesis and study of the processes of biochemical action of the coordination compound of cobalate II-nitrate with quinazolin-4-one, 6-benzylaminopurine on the Morus alba variety of Moraceae plants

Hair growth-promoting effects of Enz_MoriL on human dermal papilla cells through modulation of the Wnt/β-Catenin and JAK-STAT signaling pathways.

Enz_MoriL is a naturally occurring substance extracted from the leaves of Morus alba L. through enzymatic conversion. Historically, M. alba L. has been recognized for its potential to promote hair regrowth. However, the precise mechanism by which Enz_MoriL affects human hair follicle dermal papilla cells (hDPCs) remains unclear. The aim of this study was to investigate the molecular basis of Enz_MoriL's effect on hair growth in hDPCs. Interferon-gamma (IFN-γ) was used to examine the effects of Enz_MoriL on hDPCs during the anagen and catagen phases, as well as under conditions mimicking alopecia areata (AA). Enz_MoriL demonstrated the ability to promote cell proliferation in both anagen and catagen stages. It increased the levels of active β-catenin in the catagen stage induced by IFN-γ, leading to its nuclear translocation. This effect was achieved by increasing the phosphorylation of GSK3β and decreasing the expression of DKK-1. This stimulation induced proliferation in hDPCs and upregulated the expression of the Wnt family members 3a, 5a, and 7a at the transcript level. Additionally, Enz_MoriL suppressed JAK1 and STAT3 phosphorylation, contrasting with IFN-γ, which induced them in the catagen stage. In conclusion, Enz_MoriL directly induced signals for anagen re-entry into hDPCs by affecting the Wnt/β-catenin pathway and enhancing the production of growth factors. Furthermore, Enz_MoriL attenuated and reversed the interferon-induced AA-like environment by blocking the JAK-STAT pathway in hDPCs.

Analysis of particulate matter (PM) trapped by four different plant species in an urban forest: Quantification and characterization

Urban forests provide a series of ecosystem services, as recognized from the European 2030 Forestry Strategy; among them, they have the ability to capture particulate matter (PM) from the atmosphere, retaining it on leaves surface. This study analyses the efficiency in PM capturing of four broadleaved species: Celtis australis, Fraxinus ornus, Morus alba and Tilia cordata, set in an experimental urban forest in the city of Reggio Emilia (Italy). The airborne particles have been quantitatively and qualitatively analysed through SEM/x-EDS. Furthermore, the role played by leaf micromorphology in the efficiency of these four species to intercept PM has been assessed. Considering the total average number of particles trapped and collected by leaves for three years (from 2019 to 2021), F. ornus showed the highest capture-capacity (5.0 ± 3.1 × 103 particles/mm2), collecting 49 % more particles than Celtis australis, 86 % more than Tilia cordata and 135 % more than Morus alba. Qualitative analyses on PM have found that the majority (from 68 % to 74 %) of total particles show a diameter lower than 2,5 μm. Leaf micromorphology proved to be important, however it was no possible to identify a micromorphological trait more influential than others in determining the ability of the species to capture PM. The particles trapped by leaves were mainly rich in non-toxic or low-toxic elements, but some of them can be considered potentially dangerous for human health and the environment for the presence of Ni, Cr and Ba. The PCA highlights as the principal source of pollution in this study seems to be the erosion soil, followed by vehicle traffic. These results can give useful knowledge to understand what the contribution of different plants species in PMs retention ability is, also identified as a regulation ecosystem service.

Pharmacokinetic studies, molecular docking, and molecular dynamics simulations of phytochemicals from Morus alba: a multi receptor approach for potential therapeutic agents in colorectal cancer.

This study explores the therapeutic potential of phytochemicals derived from Morus alba for colorectal cancer (CRC) treatment. Colorectal cancer is a global health concern with increasing mortality rates, necessitating innovative strategies for prevention and therapy. Employing in silico analysis, molecular docking techniques (MDT), and molecular dynamics simulations (MDS), the study investigates the interactions between Morus alba-derived phytochemicals and key proteins (AKT1, Src, STAT3, EGFR) implicated in CRC progression. ADME/T analysis screens 78 phytochemicals for drug-like and pharmacokinetic properties. The study integrates Lipinski's Rule of Five and comprehensive bioactivity assessments, providing a nuanced understanding of Morus alba phytoconstituent's potential as CRC therapeutic agents. Notably, 14 phytochemicals out of 78 emerge as potential candidates, demonstrating oral bioavailability and favorable bioactivity scores. Autodock 1.5.7 is employed for energy minimization followed by molecular docking with the highest binding energy observed to be -11.7kcal/mol exhibited by Kuwanon A against AKT1. Molecular dynamics simulations and trajectory path analysis were conducted between Kuwanon A and AKT1 at the Pleckstrin homology (PH) domain region (TRP80), revealing minimal deviations. In comparison to the standard drug Capivasertib, the phytochemical Kuwanon A emerges as a standout candidate based on computational analysis. This suggests its potential as an alternative to mitigate the limitations associated with the standard drug. The research aims to provide insights for future experimental validations and to stimulate the development of Kuwanon A as a novel, effective therapeutic agent for managing colorectal cancer.

Neurite outgrowth promotion in PC12 cells by 24(R)-ethyllophenol from Morus alba.

We examined the mechanism by which 24(R)-ethyllophenol (MAB28) isolated from the branches of Morus alba caused neurite outgrowth in rat pheochromocytoma cells (PC12). MAB28 significantly promoted neurite outgrowth to a similar degree as the positive control, nerve growth factor (NGF). After incubation with MAB28 in PC12 cells, phosphorylation of extracellular signal-regulated kinase, p38 mitogen-activated protein kinase, and cyclic AMP response element-binding protein was detected, but the time course of phosphorylation was different from that induced by NGF. The expression of chloride intracellular channel protein 3 (CLIC3) was significantly decreased by MAB28. 5-Nitro-2-(3-phenylpropylamino)-benzoic acid (NPPB), an outward rectifying chloride channel inhibitor, significantly promoted neurite outgrowth in PC12 cells. These data suggested that MAB28 could induce neurite outgrowth by downregulating CLIC3 expression.

Morphological variation and abnormalities in two species of Typhlodromus Scheuten (Acari: Phytoseiidae)&lt;/p &gt;

Typhlodromus (Anthoseius) bagdasarjani Wainstein &amp; Arutunjan and T. (A.) khosrovensis Arutunjan have been frequently recorded from Iran. While investigating the population dynamics of these two predatory mite species on white mulberry in 2022 and 2023, in Tehran (Iran), our study unveiled intriguing morphological abnormalities and variations. Specifically, variations were observed in the number of dorsal shield solenostomes, insertion of ventrianal setae, shape of spermatheca and length of peritreme. In this context, detailed re-descriptions for both species are presented, accompanied by documentation of the observed abnormalities and variations.

Evaluation of Mediterranean Tree Leaves as Valuable Biomass of Digestive Enzymes and Bacterial Inhibitors in the Concept of Circular Bioeconomy

This study aspires to evaluate the antibacterial and inhibitory effects of carbohydrate digestive enzymes in tree leaves that are widely distributed in the Mediterranean region. Leaves were sequentially extracted with solvents of increasing polarity. The results demonstrated a wide range of phenolic (3.5–770.7 mg gallic acid equivalent g−1) and flavonoid (0.2–321.3 mg catechin equivalent g−1) contents in leaf extracts. The minimum inhibitory and bactericidal concentration of leaf extracts was determined for six bacteria using the broth microdilution method. The polar extracts of carob, lentisk, and white mulberry leaves exerted strong antibacterial potency against Gram-positive bacteria, while the susceptibility of Escherichia coli on relative apolar extracts of carob, fig, and olive leaves was also observed. In parallel, the inhibitory effects of leaf extracts on carbohydrate digestive enzymes were evaluated. A robust inhibition of α-glucosidase was found for carob and lentisk leaf extracts, followed by extracts produced by white mulberry and olive leaves. Carob and lentisk leaves also act as a-amylase inhibitors at high concentrations. Overall, this study provides valuable data for the nutraceutical value of the “forgotten” treasure of Mediterranean tree leaves and assesses these plants as potential sources of antibacterial and carbohydrate digestive enzyme inhibitory agents for drug discovery.

Effect of Mulberry Stalk Biochar on Soil Nutrients Status and Productivity of Mulberry (Morus alba L.)

Biochar as an organic amendment improves soil attributes, with a potentially significant effect on soil fertility. The main objective of this study was to quantify the effect of biochar addition on nutrients status in mulberry cultivated soil. A field experiment was conducted in the mulberry crop to know the effect of soil application of mulberry stalk biochar on nutrients build up in soil at farmer’s field at Sidlaghatta (TQ), Chikkaballapura District during 2020-2021 (rabi2020, summer and kharif-2021). A randomized block design was employed with eight treatments replicated thrice T1: Control (NPK375:140:140 kg ha-1 alone) T2: POP (FYM (25 t ha-1) + NPK 375:140:140 kg ha-1) T3: Soil application of biochar @ 5 t ha-1T4: Soil application of biochar @ 7.5 t ha-1 T5: Soil application of biochar @ 10 t ha-1T6: Soil application of biochar @ 5 t ha-1 + FYM @ 10 t ha-1T7: Soil application of biochar @7.5 t ha-1 + FYM @ 10 t ha-1T8: Soil application of biochar @ 10 t ha-1 + FYM @ 10 t ha-1NPK is common for all the treatments. Combined soil application of biochar @ 10 t ha-1 and FYM @ 10 t ha-1 (T8) increased the leaf yield (13.07 t ha-1) by 21 % over control (10.45 t ha-1 Significantly higher soil primary, secondary and micronutrients were recorded in T8 (soil application of biochar @ 10 t ha-1 + FYM @ 10 t ha-1) followed by treatment T7 which received soil application of biochar @ 7.5 t ha-1 + FYM @ 10 t ha-1. The lower soil primary, secondary and micronutrients were recorded in T1 which is devoid of biochar. The findings revealed that utilization of mulberry stalk as a biochar has positive effect on the improvement of nutrient status and it could partly replace chemical fertilizers and promote organic farming in a circular economy concept.

First report of the damaging pest Meloidogyne enterolobii parasitizing mulberry (Morus alba L.) in Vietnam.

Mulberry (Morus alba L.) is highly important crop in Vietnam, playing a key role in the country's economy through sericulture, food supply, pharmaceuticals, and beverage industries (Nguyen et al., 2018; Rohela et al., 2020). Recently, many mulberry-growing areas in Lam Dong, Vietnam have reported severe symptoms associated with nematode infection, including yellowing leaves, stunted growth, and severe root galling, leading to a significant decline in mulberry productivity. From April to December 2022, twenty soil and root samples from mulberry-growing areas in Lam Dong (Da Teh: 11°28'48.11"N; 107°28'23.74"E elevation: 133m; Lam Ha 11°48'25.13"N; 108°14'7.13"E elevation: 848m) were collected to uncover the presence of Meloidogyne enterolobii parasitizing mulberry in Vietnam. One nematode population was randomly selected for characterizing in this study among analyzed nematode populations. Females were extracted from heavily galled roots (Fig. S1) from a single mulberry tree in Lam Dong, Vietnam, using a needle and forceps (Subbotin et al., 2021). The perineal patterns of adult females (n = 10) have an oval shape, with clearly visible phasmids, along with a prominently high and squared dorsal arch. The striae are smooth and coarse, while the perivulval region remains devoid of striae. The lateral lines appear indistinct, and the tail tip is easily observable. Morphometric measurements were as follows: body length = 585 ± 78 (464-724) µm, body width = 367 ± 75 (271-529) µm, neck length = 221.5 ± 30.7 (167-269.6) µm, stylet length = 13.1 ± 1.2 (11.4-15.1) µm, vulva-slit length 16.3±2.3 (10.4-18) µm, vulva-anus distance = 16.8±3.0 (11.4-18) µm, anus-tail tip distance = 10.3±2.1 (6.9-14.2) µm, interphasmidial distance = 15.9 ± 3.7 (10.3-23.4) µm. The morphology of this nematode population is highly in agreement with the original description of M. enterolobii (Yang & Eisenback, 1983). This population was also identified using the D2-D3 of 28S rRNA and 18S rRNA (Powers et al., 2017; Subbotin et al., 2006) regions. The D2-D3 of 28S rRNA sequences from this study (accession numbers: OR889633) exhibited 99.5-99.8% similarity to the sequences of M. enterolobii from GenBank (accession numbers: OR214950 and ON496981). While the 18S rRNA sequences (accession numbers: OR896547) showed 99.2-99.3% similarity to the sequences of M. enterolobii from GenBank (accession numbers: MZ955995, MZ531901, and MW488150). To carry out Koch's postulates, 2000 J2s from collected M. enterolobii egg masses (initial population) were inoculated on two-month-old plantlets of mulberry (n = 6), planted on 2L pots within a screenhouse, non-inoculated plantlets (n=6) served as negative controls. After 90 days post-inoculation, nematode reproduction factors (RF = final density (nematodes were extracted from the whole root system and corresponding soil samples (Subbotin et al., 2021)) / initial population) and root damage symptoms were evaluated. The inoculated plantlets exhibited consistent yellowing leaves, stunting, and root galling symptoms (Fig. S1), mirroring observations from the field, with an average RF of 11.5. Control plants displayed no symptoms. Root-knot nematodes extracted from the roots were identified as M. enterolobii through molecular analyses of D2-D3 of 28S and 18S rRNA regions (GenBank accession numbers: OR889634 (D2-D3 of 28S) and OR896548 (18S)), thereby confirming that mulberry acts as a host for M. enterolobii. Currently, this nematode has been reported to be associated with two different host plants, including guava (Trinh et al., 2022) and pomelo (Le et al., 2023). Our discovery marks the first documented case of Meloidogyne enterolobii parasitizing mulberry in Vietnam. While the impact on mulberry productivity remains to be really important for sericulture food supply, pharmaceuticals, and beverage industries; the aggressive nature of M. enterolobii, as observed in the field and confirmed by the screenhouse tests, raises concerns about potential economic losses in mulberry production. Therefore, further investigations are needed to assess the extent of M. enterolobii infestation in mulberry orchards and to develop effective control measures to safeguard the sustainability of mulberry cultivation in Vietnam.

Network Pharmacology Prediction and Metabolomics Validation of the Novel Targets of Morus alba L. against High-Fat Diet-Induced Diabetes Mellitus in C57/6J Mice

Diabetes mellitus (DM) is an endocrine-metabolic disorder that has limited approaches to treat effectively. Morus alba L., also known as mulberry, is a well-known medicinal plant, and its branch bark has shown hypoglycemic activity. It is rich in antioxidant and anti-inflammatory ingredients. In this study, we used metabolomics combined with network pharmacology to investigate the molecular mechanism and potential key targets of mulberry branch bark powder (MBBP) for treating DM. Serum metabolomics was performed to analyze the differences in metabolites and enrich metabolic pathways. Network pharmacology, based on systems biology tools, was applied to generate the pathway-target-compound network. Integrated analyses were then used to screen for key targets. To verify the obtained key targets, we used a molecular docking method and experimental validation. Our findings revealed that thirty-five endogenous metabolites contributed to the therapeutic impact of MBBP against DM. The analysis of 10 hub genes in the compound-target network partially supported the enrichment of metabolic pathways. Further analysis focused on two compounds (eugenol and mulberrofuran A) and three key targets (NOS2, MAOA, and CYP1A1). This study explores the active compounds of MBBP against DM and provides a novel perspective for improving DM treatment based on key targets.

Characterisation of fruit trees in the EU: a tool for crop‐based survey of Union quarantine pests

Abstract This technical report was prepared in the context of the EFSA mandate on plant pest surveillance (M‐2020‐0114) at the request of the European Commission. Its purpose is to support Member States in planning and preparing crop‐based surveys of Union quarantine pests in line with Commission Implementing Regulation (EU) 2019/2072. In particular, the report includes a list of species reported as hosts of the regulated pests targeted by the call: GP/EFSA/PLANTS/2022/05 – 'Development of crop‐based survey tools for plant pests of fruit trees, conifers, and palms and ornamentals in the EU' – Lot 1. Additionally, a classification of their occurrence in the EU is provided. The report provides basic information regarding the cultivation in the EU of 19 species of fruit trees widely cultivated in the EU, hosting at least one pest of the call in the families of the Ebenaceae, i.e. persimmon (Diospyros kaki L. f.); Ericaceae, i.e. bilberry (Vaccinium myrtillus L.), high‐bush blueberry (Vaccinium corymbosum); Lythraceae, i.e. pomegranate (Punica granatum L.); Moraceae, i.e. silkworm mulberry (Morus alba), fig (Ficus carica L.); Rosaceae, i.e. apple (Malus domestica Borkh.), pear (Pyrus communis L.), quince (Cydonia oblonga Mill.), plum (Prunus domestica), almond (Prunus dulcis Miller D.A. Webb.), apricot (Prunus armeniaca L.), sour cherry (Prunus cerasus), peach (Prunus persica (L.) Batsch), sweet cherry (Prunus avium L.), Japanese plum (Prunus salicina Lindl.), loquat (Eriobotrya japonica (Thunb.) Lindl.); Rutaceae, i.e. bitter orange (Citrus × aurantium L.); and Vitaceae, i.e. grape (Vitis vinifera). For the selection of cultivated fruit‐tree species, information on the main phenological stages, management practices, agroecosystems, climate suitability and distribution, regulatory requirements and trade of key commodities are provided.

Application of Natural Surfactant from Morus alba, Soapnut, Sapindus rarak for Enhanced Oil Recovery – Critical Review

This literature discusses the importance of green surfactants in the oil and gas industry as one of the injection types in EOR. The use of green surfactants is good for protecting the environment and marine biodiversity from conventional chemical surfactants. The injection of green surfactants uses natural materials from Soapnut, Mulberry, and Sapindus rarak. These three types of plants are abundant in Indonesia and contain saponin as a material in making good surfactants. Saponin is an amphipathic glycoside that can produce foam when shaken vigorously in a solution. The foam produced by saponin can increase the sweep efficiency in oil wells to increase production. The writing method used in this article is based on scientific literature that discusses the characteristics and physical properties of Soapnut, Mulberry, and Sapindus rarak. The IFT calculation for Soapnut between water and oil reduced from 19 dyne/cm to 2.5 dyne/cm as the surfactant concentration increased from 0 to 12 (w/w%). Mulberry from 43.9 to 17.9 dyne/cm and Sapindus rarak from 3.07 mN/m to 0.96. These natural surfactants enhance water flooding efficiency by 19.6% OOIP, reduces shale contact angle from 52.44 to 19.66, emphasizing natural surfactants oil recovery potential with environmental benefits.

Molecular phylogeny and secondary structure analysis of hop stunt viroid (HSVd) associated with Mulberry (Morus alba) in India.

Hop stunt viroid (HSVd), a small, single stranded, circular, non-coding infectious RNA known to cause infection in various economically important crop plants. In the present investigation, a study was conducted in the southern part of Karnataka districts of India to detect the possible association of HSVd infection in mulberry plants. A total of 41 mulberry plants showing typical viroid-like symptoms along with asymptomatic samples were collected and screened using conventional Reverse Transcriptase Polymerase Chain Reaction (RT-PCR) using a specific set of HSVd-Fw/ HSVd-Re primers. Out of 41 samples, the study confirmed the presence of HSVd in six samples of mulberry collected from Ramanagara (1 sample), Chikkaballapur (3 samples) and Doddaballapura (2 samples) regions with an expected HSVd amplicon size of ∼ 290-300 nucleotides. The mechanical transmission of HSVd was also confirmed on cucumber (cv. Suyo) seedlings through bioassay, which was reconfirmed by RT-PCR. The amplicons were cloned, sequenced, and the representative nucleotide sequences were deposited in the NCBI GenBank. Subsequently, molecular phylogenetic analysis showed that HSVd mulberry isolates from this study were most closely related to grapevine isolates, indicating a common origin. On the other hand, it was shown to belong to a different group from mulberry isolates so far reported from Iran, Italy, Lebanon, and China. The secondary structure analysis of HSVd mulberry Indian isolates exhibited substitutions in the terminal left, pathogenicity, and variable regions compared to those of the Indian grapevine isolates. As far as this study is concerned, HSVd was detected exclusively in some mulberry plants with viral-like symptoms, but the pathogenesis and symptom expression needs to be further investigated to establish the relationship between HSVd and the disease symptoms in the mulberry plants.

Characterizations of White Mulberry, Sea-Buckthorn, Garlic, Lily of the Valley, Motherwort, and Hawthorn as Potential Candidates for Managing Cardiovascular Disease-In Vitro and Ex Vivo Animal Studies.

Cardiovascular diseases are a broadly understood concept focusing on vascular and heart dysfunction. Lack of physical exercise, type 2 diabetes, obesity, hypertension, dyslipidemia, thromboembolism, and kidney and lung diseases all contribute to the development of heart and blood vessel dysfunction. Although effective and important, traditional treatment with diuretics, statins, beta blockers, calcium inhibitors, ACE inhibitors, and anti-platelet drugs remains a second-line treatment after dietary interventions and lifestyle changes. Scientists worldwide are still looking for an herbal product that would be effective and free from side effects, either taken together with or before the standard pharmacological intervention. Such herbal-originated medication therapy may include Morus alba L. (white mulberry), Elaeagnus rhamnoides (L.) A. Nelson (sea-buckthorn), Allium sativum L. (garlic), Convallaria majalis L. (lily of the valley), Leonurus cardiaca L. (motherwort), and Crataegus spp. (hawthorn). Valuable herbal raw materials include leaves, fruits, seeds, and even thorns. This short review focuses on six herbs that can constitute an interesting and potential therapeutic option in the management of cardiovascular disorders.

Comparative Physio-Biochemical and Transcriptome Analyses Reveal Contrasting Responses to Magnesium Imbalances in Leaves of Mulberry (Morus alba L.) Plants.

Magnesium (Mg) deficiency is a major factor limiting the growth and development of plants. Mulberry (Morus alba L.) is an important fruit tree crop that requires Mg for optimal growth and yield, especially in acid soils. However, the molecular mechanism of Mg stress tolerance in mulberry plants remains unknown. In this study, we used next-generation sequencing technology and biochemical analysis to profile the transcriptome and physiological changes of mulberry leaves under different Mg treatments (deficiency: 0 mM, low: 1 mM, moderate low: 2 mM, sufficiency: 3 mM, toxicity: 6 mM, higher toxicity: 9 mM) as T1, T2, T3, CK, T4, T5 treatments, respectively, for 20 days. The results showed that Mg imbalance altered the antioxidant enzymatic activities, such as catalase (CAT), peroxidase (POD), and superoxide dismutase (SOD), and non-enzymatic, including soluble protein, soluble sugar, malondialdehyde (MDA), and proline (PRO), contents of the plant. The Mg imbalances disrupted the ultrastructures of the vital components of chloroplast and mitochondria relative to the control. The transcriptome data reveal that 11,030 genes were differentially expressed (DEGs). Genes related to the photosynthetic processes (CAB40, CAB7, CAB6A, CAB-151, CAP10A) and chlorophyll degradation (PAO, CHLASE1, SGR) were altered. Antioxidant genes such as PER42, PER21, and PER47 were downregulated, but DFR was upregulated. The carbohydrate metabolism pathway was significantly altered, while those involved in energy metabolism processes were perturbed under high Mg treatment compared with control. We also identified several candidate genes associated with magnesium homeostasis via RT-qPCR validation analysis, which provided valuable information for further functional characterization studies such as promoter activity assay or gene overexpression experiments using transient expression systems.