The aim of this study was to evaluate and compare the varroacidal efficacy and mite mortality dynamic during autumn treatment of honey bee colonies situated at two exper-imental areas, treated with three available authorized veterinary medicinal substances. In the autumn of 2017 clinical studies were conducted on the efficiency of the three acari-cidal combinations (AC) - one, based on 3. 6 mg flumethrin/strip (AC-1) and two contents essential oils (first one with composition: 5 g thymol and 2 g peppermint oil/plate (AC-2), and second one with composition: 4 g thymol and 2 g peppermint oil/plаte (AC-3). For control treatment we used the product containing coumaphos and additive with oxalic acid. After 35 days of flumethrin-strips exposure, an efficiency of 94.5% and 87.82% were achieved in the apiaries in Boychinovtsi and Zlatiya, respectively. The efficiencies of the other tested combinations - 5 g thymol/ and 2 g peppermint oil plate and 4 g thymol and 2 g peppermint oil /plate for 45 days were also high in the apiary in Zlatiya (AC-2 - 97% and for AC-3 - 95%) and in Boychinovtsi were 91% for AC-2 and 80% for AC-3, respectively. The results of the experiments showed the absence of resistance to the tested substances.