We observe ripening suppression in aligned InGaAs quantum dots (QDs) along multiatomic steps on a vicinal (100) GaAs substrate. By varying the growth interruption times, a study of QD morphologies by atomic force microscope reveals a clear Ostwald ripening suppression effect on QD formation. On the other hand, we observe a regular ripening for InGaAs QDs on an exact (100) substrate. In addition, n-QD chains aligned along multiatomic steps are observed. An inhomogeneously broadened photoluminescence (PL) spectrum with larger spectral width is obtained for a QD ensemble on a vicinal (100) substrate as compared to those on an exact (100) substrate although statistical analyses of QD size distributions show similar size dispersions. The spectral broadening is attributed to the lateral quantum coupling of aligned n-QD chains. PL spectra for QDs on vicinal (100) substrates show a larger rate of blueshift with increasing interruption times, which is interpreted as a lack of the PL compensation effect by size enlargement of the QDs during growth interruption. Thus the result supports the ripening suppression effect which is observed in the morphology study.