Bitemark analysis of humans and domestic animals is by far the most demanding and complicated part of forensic dentistry. This study compared and analyzed the bite mark impression patterns of 34 domestic animals and 16 humans. Bitemark comparison included comparing the bitemark of the suspected biter's distinctive dental features to determine whether a link exists between the two sets of evidence. Oval or elliptical arch shape of humans, circular to oval shape of dog, circular arch shape of cat was noted. The incisor morphology for humans was rectangular shape, oval to rectangular for dogs, circular for cats was observed. The inter canine distance of humans measured on dental cast ranged from 22–40 mm with a standard deviation of 5.0 and mean of 33.2, 32–50 mm with a standard deviation of 4.11 and mean of 38.44 for dogs, 11.80 to 18.24 mm with a standard deviation of 2.44 and mean of 16.14 for cats. Intercanine distance, arch shape are few, simple reliable parameter to differentiate between bite marks that are produced by humans and domestic animals. The outcome of the study serves and helps the forensic fraternity of different expertise such as forensic odontologist, forensic pathologists, veterinarians, biologists, crime investigators, and others.